• Journal of Korea Port Economic Association
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• v.32 no.1
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• pp.17-28
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• 2016

This study examines the cruise course network structure in the Asian regions and the centrality of ports using social network analysis (SNA). For network analysis of Asian cruise courses, a data network of cruise courses was constructed using data on courses of cruise ships operating in Asian ports collected from the reports of the Cruise Lines International Associations.There are 249 nodes or ports of ship companies that provide cruise courses to Asia between from October 2015 to June 2016, and these nodes connect 545 ports. Density analysis based on ports where cruise ship companies operated cruise ships showed that, from October 2015 to June 2016, the density was 0.009, which was lower than the average of global port network density (2006 to 2011) and railroad network density. In addition, was calculated to be, which means that connection with all ports was possible through 2,180 steps. In the analysis of the Asian cruise course network centrality, Singapore ranked first in both out-degree and in-degree in connection centrality, followed by Hong Kong, Shanghai, Ho Chi Minh, and Keelung. Singapore also ranked first in the result betweenness centrality analysis, followed by Penang, Dubai, and Hong Kong. From October 2015 to June 2016, the port with the highest Eigenvector centrality was Hong Kong, followed by Ho Chi Minh, Singapore, Shanghai, and Danang. In the case of the domestic ports Incheon, Busan, and Jeju, connection centrality, betweenness centrality, and Eigenvector centrality all ranked lower than their competitor Chinese ports.

• Korean Journal of Human Ecology
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• v.9 no.4
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• pp.75-81
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• 2006

In this study on the recipe for Yakgwa in Jeonbuk province, a survey of the ingredients, the quantity and the recipe for Yakgwa were conducted by an interview with the notable maker of it in 9 regions ; Jeonju, Imshil, Jangsu, Jeongup, Namwon(Samaemyeon & Dukgwamyeon), Wanju, Gochang, Iksan, away from each other. The hardness testing and the sensory evaluation of Yakgwa were carried out to compare with the control group and Jeonbuk Yakgwa. The results of this study are as follows ; 1. Common ingredients of Yakgwa examined in 9 regions were flour, sesame oil or soybean oil, honey or sugar, ginger juice, alcoholic drinks and etc. The quantity of oil (sesame or soybean) per kilogram of flour was as follows between one-half and one cup of oil, between one and three cups of honey or sugar, between one-half and one cup of ginger juice and between one-half and one cup of alcoholic drinks. Making of Yakgwa in Jeonju and Imshil area, knead flour with the liquid ingredients which are mixed together before. In other areas, after sifting the mixed flour with sesame oil, and then knead the remaining with the sifted. 2. The result of the hardness testing of Yakgwa was that it is higher in this order, Iksan, Imshil, the control group, Jeongup, Jeonju. With while the difference of syrup used in each of them, the result of hardness testing in the same order shows that the component of Yakgwa has more great effect on the hardness than a kind of syrup on which the result by using syrup before&after cooking. And in the sensory evaluation, the surface color of Iksan Yakgwa was the most bright of all, and the shininess was of Imshil and the control group, the cracked level, the softness, the sweet taste and the oily taste of Jeonju Yakgwa was better than other Also, the overall preference, though less meaningful, was same result.

• Korean Journal of Heritage: History & Science
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• v.47 no.3
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• pp.60-85
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• 2014

The study is for tracing the mutational process, considering the transformational process of wooden chamber tombs in Gyoungju region during 2~6 century. In addition, class features and the meaning of tombs were studied. First of all, the wooden chamber tombs were classified into 10 different styles(1A~IVC), based on the filling materials, the ways of structure, and the structure of burial chamber. There were only one or two tombs that belongs to the highest classes 'Ga'~'Da' in each period, which has the numeral features. Furthermore, the styles of the central wooden chamber tombs according to the period and the region have distinctive features and have changed so far. It shows that there are the rank among the tombs in the regions. In other words, regarding the ranks of the class 'Ga'~'Cha', there were strict rules and the limitations on the size of the tombs and the number and the quality of the burial relics. It means that the inner-outer size or the structure of the tombs and the number, quality of the burial goods depended on the buried person's birth or social classes, or achievements. Based on Shila social classes, the class 'Ga' tombs were for the highest classes when considered the size, burial relics, and the quality. The class 'Na' and 'Da' tombs were for the higher classes, 'Ma' for middle classes, 'Ba', 'Sa' for lower classes. In addition, 'Ah', 'Ja' tombs were for higher commoner, 'Cha' for lower commoner. Thus, class 'Ga'~'Sa' is the leading class of Shila.

• Molecules and Cells
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• v.38 no.3
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• pp.210-220
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• 2015

Athletic performance is an important criteria used for the selection of superior horses. However, little is known about exercise-related epigenetic processes in the horse. DNA methylation is a key mechanism for regulating gene expression in response to environmental changes. We carried out comparative genomic analysis of genome-wide DNA methylation profiles in the blood samples of two different thoroughbred horses before and after exercise by methylated-DNA immunoprecipitation sequencing (MeDIP-Seq). Differentially methylated regions (DMRs) in the pre-and post-exercise blood samples of superior and inferior horses were identified. Exercise altered the methylation patterns. After 30 min of exercise, 596 genes were hypomethy-lated and 715 genes were hypermethylated in the superior horse, whereas in the inferior horse, 868 genes were hypomethylated and 794 genes were hypermethylated. These genes were analyzed based on gene ontology (GO) annotations and the exercise-related pathway patterns in the two horses were compared. After exercise, gene regions related to cell division and adhesion were hypermethylated in the superior horse, whereas regions related to cell signaling and transport were hypermethylated in the inferior horse. Analysis of the distribution of methylated CpG islands confirmed the hypomethylation in the gene-body methylation regions after exercise. The methylation patterns of transposable elements also changed after exercise. Long interspersed nuclear elements (LINEs) showed abundance of DMRs. Collectively, our results serve as a basis to study exercise-based reprogramming of epigenetic traits.

• Molecules and Cells
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• v.24 no.3
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• pp.364-371
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• 2007

The tumor suppressor gene Ras association domain family 1A (RASSF1A) is highly methylated in a wide range of human sporadic tumors. The current study investigated the hypermethylation of RASSF1A, the expression of RASSF1A protein, and the correlation between these and the clinicopathological features of gallbladder (GB) cancer in Korean patients. Formalin-fixed, paraffin-embedded tumors and non-neoplastic GB tissues (22 carcinomas, 8 adenomas, 26 normal epithelia) were collected from patients who had undergone surgical resection. The methylation status of two regions of the RASSF1A CpG island was determined by methylation-specific PCR (MSP), and the expression of RASSF1A protein was examined by immunohistochemistry using tissue microarrays. The K-RAS mutation was analyzed by direct sequencing. Methylation of the RASSF1A promoter (region 1) was detected in 22.7% (5/22) of carcinomas, 12.5% (1/8) of adenomas, and 0% (0/26) of normal gallbladder epithelia (P = 0.025). Methylation of the first exon (region 2) was found in 36.4% (8/22) of carcinomas, 25.0% (2/8) of adenomas, and 8.0% (2/26) of normal gallbladder epithelia (P = 0.038). K-RAS mutations were present in 4.5% (1/22) of carcinomas and 25% (2/8) of adenomas. RASSF1A methylaton was not associated with clinicopathological factors or K-ras mutation. Reduction or loss of RASSF1A expression was observed in most methylated adenocarcinomas. Three RASSF1A-expressing human biliary tract cancer cell lines examined contained unmethylated promoters and exons 1. These results suggest that downregulation of RASSF1A expression by DNA hypermethylation may be involved in GB carcinogenesis.

• Journal of Microbiology and Biotechnology
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• v.18 no.3
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• pp.404-409
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• 2008

The brown-rot basidiomycete Fomitopsis palustris is known to degrade crystalline cellulose (Avicel) and produce three major cellulases, exoglucanases, endoglucanases, and ${\beta}$-glucosidases. A gene encoding endoglucanase, designated as cel12, was cloned from total RNA prepared from F. palustris grown at the expense of Avicel. The gene encoding Cel12 has an open reading frame of 732 bp, encoding a putative protein of 244 amino acid residues with a putative signal peptide residing at the first 18 amino acid residues of the N-terminus of the protein. Sequence analysis of Cel12 identified three consensus regions, which are highly conserved among fungal cellulases belonging to GH family 12. However, a cellulose-binding domain was not found in Cel12, like other GH family 12 fungal cellulases. Northern blot analysis showed a dramatic increase of cel12 mRNA levels in F. palustris cells cultivated on Avicel from the early to late stages of growth and the maintenance of a high level of expression in the late stage, suggesting that Cel12 takes a significant part in endoglucanase activity throughout the growth of F. palustris. Adventitious expression of cel12 in the yeast Pichia pastoris successfully produced the recombinant protein that exhibited endoglucanase activity with carboxymethyl cellulose, but not with crystalline cellulose, suggesting that the enzyme is not a processive endoglucanase unlike two other endoglucanases previously identified in F. palustris.

• The Plant Pathology Journal
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• v.31 no.3
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• pp.212-218
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• 2015

In this study, we developed a species-specific PCR assay for rapid and accurate detection of three Xanthomonas species, X. axonopodis pv. poinsettiicola (XAP), X. hyacinthi (XH) and X. campestris pv. zantedeschiae (XCZ), based on their draft genome sequences. XAP, XH and XCZ genomes consist of single chromosomes that contain 5,221, 4,395 and 7,986 protein coding genes, respectively. Species-specific primers were designed from variable regions of the draft genome sequence data and assessed by a PCR-based detection method. These primers were also tested for specificity against 17 allied Xanthomonas species as well as against the host DNA and the microbial community of the host surface. Three primer sets were found to be very specific and no amplification product was obtained with the host DNA and the microbial community of the host surface. In addition, a detection limit of $1pg/{\mu}l$ per PCR reaction was detected when these primer sets were used to amplify corresponding bacterial DNAs. Therefore, these primer sets and the developed species-specific PCR assay represent a valuable, sensitive, and rapid diagnostic tool that can be used to detect three specific pathogens at early stages of infection and may help control diseases.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.20 no.3
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• pp.214-216
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• 1998

In the treatment of maxillary lesion including a maxillary sinuses, most of oral and maxillofacial surgeons have used the vestibular incision or the Weber-Fergusson incision. However, the vestibular incision has disadvantage which it provides a rather limited exposure and the Weber-Fergusson incision leaves visible scar in the midface. Furthermore, because the scar is confined on unilateral side only, the technique is hesitated to apply especially in children, younger patients and women. Since Casson first introduced midfacial degloving technique, this approach has been used frequently to treat the lesion on nasal cavity, nasopharynx, skull base and paranasal sinuses by ENT surgeons. But, we think this technique can be used usefully in oral and maxillofacial regions. So, we experienced favorable results which it provided a proper exposure, no visible facial scar and it could be used on bilateral midfacial lesions.

• Journal of the Earthquake Engineering Society of Korea
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• v.14 no.3
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• pp.39-48
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• 2010

Improved seismic performances of RC bridges can be attained by sufficient ductilities of piers, which can be obtained by providing sufficient lateral confinements to the plastic hinge regions of piers. The cross sectional shape and the amount of lateral reinforcements are key parameters in the determination of effective confinements. Even though identical amounts of lateral reinforcement are provided, the effective confinement differs due to different spacing, arrangements, hook details and so on. Unlike circular sections in which confinement is exerted by mere hoop reinforcements, cross-ties are arranged in square or rectangular sections to enhance the effective confinements. The stress-strain relationship of confined concrete is varied by how to consider these cross-ties. In this study, the stress-strain relationships of confined concrete with cross-ties are investigated experimentally and their mechanical characteristics are estimated by comparison with other empirical equations.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.36 no.6
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• pp.543-547
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• 2010

Plasmacytoma is a rare malignant neoplasm in the head and neck region and comprises approximately 3% of all plasma cell tumors. This lesion is a unifocal, monoclonal, neoplastic proliferation of plasma cells that usually arises within the bone. Infrequently, it is observed in soft tissue, in which case, the term extramedullary plasmacytoma is used. Approximately 80-90% of extramedullary plasmacytomas involve the mucos-Associated-Lymphoid Tissue of the upper airways with 75% of these involving the nasal and paranasal regions. The plasmacytoma is usually detected in adult males, with an average age at diagnosis of 55 years. The male-to-female ratio is 3:1.Radiographically, the lesion may be seen as a well-defined, unilocularradioluceny with no evidence of a sclerotic border. Some investigators believe that this lesion represents the least aggressive part of the spectrum of plasma cell neoplasms that extend to multiple myeloma.Therefore, plasma cytoma is believed to have clinical importance. We report a case of extramedullary plasmacytoma in the right maxillary sinus of a 59-year-old male with review of the relevant literature.