• International Journal of Oral Biology
• /
• v.36 no.3
• /
• pp.117-122
• /
• 2011

Endothelial cells are a vital constituent of most mammalian organs and are required to maintain the integrity of these tissues. These cells also play a major role in angiogenesis, inflammatory reactions, and in the regulation of thrombosis. Angiogenesis facilitates pulp formation and produces the vessels which are essential for the maintenance of tooth homeostasis. These vessels can also be used in bone and tissue regeneration, and in surgical procedures to place implants or to remove cancerous tissue. Furthermore, endothelial cell regeneration is the most critical component of the tooth generation process. The aim of the present study was to stimulate endothelial regeneration at a site of acute cyclophosphamide (CP)-induced endothelial injury by treatment with human umbilical cord-derived endothelial/mesenchymal stem cells (hEPCs). We randomly assigned 16 to 20-week-old female NOD/SCID mice into three separate groups, a hEPC ($1{\times}10^5$ cells) transplanted, 300mg/kg CP treated and saline (control) group. The mice were sacrificed on days 5 and 10 and blood was collected via the abdominal aorta for analysis. The alanine transaminase (ALT), aspartate aminotransferase (AST), serum alkaline phosphatase (s-ALP), and albumin (ALB) levels were then evaluated. Tissue sections from the livers and kidneys were stained with hematoxylin and eosin (HE) for microscopic analysis and were subjected to immunohistochemistry to evaluate any changes in the endothelial layer. CP treatment caused a weight reduction after one day. The kidney/body weight ratio increased in the hEPC treated animals compared with the CP only group at 10 days. Moreover, hEPC treatment resulted in reduced s-ALP, AST, ALT levels compared with the CP only group at 10 days. The CP only animals further showed endothelial injuries at five days which were recovered by hEPC treatment at 10 days. The number of CD31-positive cells was increased by hEPC treatment at both 5 and 10 days. In conclusion, the CP-induced disruption of endothelial cells is recovered by hEPC treatment, indicating that hEPC transplantation has potential benefits in the treatment of endothelial damage.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2011.06a
• /
• pp.555-556
• /
• 2011

• Transactions of the Korean Society of Mechanical Engineers
• /
• v.10 no.4
• /
• pp.463-470
• /
• 1986

본 연구에서는 절삭공정 중 발생하는 공구의 접촉이탈 현상에 의하여 공작 물의 절삭된 표면중에 한회전 및 그 이전 회전에 절삭된 절삭면의 형상이 현재의 절삭 깊이에 영향을 미치는 다중재생효과(multiple regenerative effect)가 존재하는 선삭 작업에서 공구의 착탈현상을 고려한 비선형채터(nonlinear chatter)를 공작기계의 생 산성의 관점에서 절삭공정의 특성을 고려하여 해석하였으며, 수동제어기의 일종인 임 펙트댐퍼를 절삭공정에 응용하여 절삭작업중에 공작기계의 안정성향상 뿐만 아니라 생 산성의 증가효과를 규명하였다.아울러 댐퍼자체의 설계변수에 따른 채터 억제효과 를 고려하여 최적의 댐퍼를 설계하는 방법을 제시하였다.

• The Transactions of The Korean Institute of Electrical Engineers
• /
• v.59 no.12
• /
• pp.2306-2313
• /
• 2010

This paper describes a model based fault detection algorithm for an Advanced ESC System which consists of Hydraulic Control Unit (HCU) with built-in wheel pressure sensors. Advanced ESC System can be used for various value-added functions such as Stop & Go Function and Regenerative Brake Function. Therefore, HCU must have a reliable fault detection. Due to the huge amount of sensor signals, existing specific sensor based fault detection of HCU cannot guarantee the safety of vehicle. However, proposed algorithm dose not require the sensors. When model based fault detection algorithm detects severe failures of the HCU, it warns the driver in advance to prevent accidents due to the failures. For this purpose, a mathematical model is developed and validated in comparison to actual data. Simulation results and data acquired from an actual system are compared with each other to obtain the information needed for the fault detection process.

• Proceedings of the Korean Society of Machine Tool Engineers Conference
• /
• 2002.10a
• /
• pp.440-445
• /
• 2002

The characteristic equation for regenerative chatter loop including a delay element replaced by a rational function is presented by a linear differential-difference equation, accounting for the dynamics of the AMB controllers, the uncut chip thickness equation and the cutting process as well as the rigid spindle dynamics itself. The chatter stability analysis of a rigid milling spindle suspended by 5-axes active magnetic bearings(AMBs) is also performed to investigate the influences of the damping and stiffness coefficients of AMBs on the chatter free cutting conditions, as they are allowed to vary within the stable region formed by the AMB control gains. Several cutting tests varying the derivative gains of the AMB were performed to investigate the regenerative chatter vibrations, and it was concluded that the theoretical analysis results are in good consistency with the test results.

• Animal cells and systems
• /
• v.16 no.6
• /
• pp.462-468
• /
• 2012

Urodeles including newt and salamander have remarkable regenerative capacity during the postembryonic life span. Some of the unique features are the formation of the well-developed wound epidermis and the active dedifferentiation process in the early phase of regeneration. These are regarded as key events for the successful regeneration since no further regenerative activity is possible without them. In this study, we investigated the role of retinoic acid (RA) in salamander limb regeneration by blocking RA synthesis using disulfiram, an inhibitor of aldehyde dehydrogenase that oxidizes retinal to RA. Disulfiram treatment resulted in delaying the limb regeneration processes via inhibition of wound epidermis formation and dedifferentiation process. When RA was administered after disulfiram treatment, the inhibitory effect of disulfiram was rescued. In addition, disulfiram treatment after the dedifferentiation stage resulted in the mild retardation of limb regeneration, suggesting that RA might also be involved in the blastema outgrowth. Furthermore, salamander MMP-9 gene expression was also inhibited by disulfiram treatment. Collectively, our findings indicate that endogenous RA may play an important role(s) in the early phase of limb regeneration by regulating the expression of molecules responsible for the modification of intracellular and extracellular environment during salamander limb regeneration.

• Journal of the Korean Society of Propulsion Engineers
• /
• v.18 no.6
• /
• pp.88-95
• /
• 2014

Spinning process to inner wall has been applied for reducing the weight of regenerative cooling chamber of liquid propellent rocket engine. The fractures of the blanks of cylinder part and nozzle throat part have been observed during spinning processes. In order to overcome the problem, the mandrel and the blank shape have been modified, and the inner wall was successfully manufactured through the modifications. The manufactured spinning prototype of nozzle throat part was successfully bulged without cracking and necking, and it was confirmed to secure sufficient formability necessary for fabricating thrust chamber.

• International Journal of Oral Biology
• /
• v.42 no.2
• /
• pp.71-78
• /
• 2017

BMP-2 is a well-known TGF-beta related growth factor, having a significant role in bone and cartilage formation. It has been employed to promote bone formation in some clinical trials, and to differentiate mesenchymal stem cells into osteoblasts. However, it is difficult to obtain this protein in its soluble and active form. hBMP-2 is expressed as an inclusion body in the bacterial system. To continuously supply hBMP-2 for research, we optimized the refolding of recombinant hBMP-2 expressed in E. coli, and established an efficient method by using detergent and alkali. Using a heparin column, the recombinant hBMP-2 was purified with the correct refolding. Although combinatorial refolding remarkably enhanced the solubility of the inclusion body, a higher yield of active dimer form of hBMP-2 was obtained from one-step refolding with detergent. The refolded recombinant hBMP-2 induced alkaline phosphatase activity in mouse myoblasts, at $ED_{50}$ of 300-480ng/ml. Furthermore, the expressions of osteogenic markers were upregulated in hPDLSCs and hDPSCs. Therefore, using the process described in this study, the refolded hBMP-2 might be cost-effectively useful for various differentiation experiments in a laboratory.

• Journal of Periodontal and Implant Science
• /
• v.44 no.1
• /
• pp.39-47
• /
• 2014

Purpose: Peri-implantitis, a clinical term describing the inflammatory process that affects the soft and hard tissues around an osseointegrated implant, may lead to peri-implant pocket formation and loss of supporting bone. However, this imprecise definition has resulted in a wide variation of the reported prevalence; ${\geq}10%$ of implants and 20% of patients over a 5- to 10-year period after implantation has been reported. The individual reporting of bone loss, bleeding on probing, pocket probing depth and inconsistent recording of results has led to this variation in the prevalence. Thus, a specific definition of peri-implantitis is needed. This paper describes the vast variation existing in the definition of peri-implantitis and suggests a logical way to record the degree and prevalence of the condition. The evaluation of bone loss must be made within the concept of natural physiological bony remodelling according to the initial peri-implant hard and soft tissue damage and actual definitive load of the implant. Therefore, the reason for bone loss must be determined as either a result of the individual osseous remodelling process or a response to infection. Methods: The most current Papers and Consensus of Opinion describing peri-implantitis are presented to illustrate the dilemma that periodontologists and implant surgeons are faced with when diagnosing the degree of the disease process and the necessary treatment regime that will be required. Results: The treatment of peri-implantitis should be determined by its severity. A case of advanced peri-implantitis is at risk of extreme implant exposure that results in a loss of soft tissue morphology and keratinized gingival tissue. Conclusions: Loss of bone at the implant surface may lead to loss of bone at any adjacent natural teeth or implants. Thus, if early detection of peri-implantitis has not occurred and the disease process progresses to advanced peri-implantitis, the compromised hard and soft tissues will require extensive, skill-sensitive regenerative procedures, including implantotomy, established periodontal regenerative techniques and alternative osteotomy sites.

• Journal of the Optical Society of Korea
• /
• v.7 no.3
• /
• pp.160-164
• /
• 2003

Materials processing by ultrashort pulsed laser is actively being applied to micromachining technology due to its advantages with regard to non-thermal machining. In this study, materials processing with ultrashort pulses was studied by using the high repetition rate of a 800 nm Ti:sapphire regenerative amplifier. This revealed that the highly precise micromachining of metallic thin film and bulk glass with a minimal heat affected zone (HAZ) could be obtained by using near damage threshold energy. Grooves with diffraction limited sub-micrometer width were obtained with widths of 620 nm on Cr thin film and 800 nm on a soda-lime glass substrate. The machined patterns were investigated through SEM images. We also phenomenologically examined the influence of variations of parameters and proposed the optimal process conditions for microfabrication.