• 구강회복응용과학지
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• 제28권1호
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• pp.37-46
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• 2012

치주치료의 궁극적 목표는 치주질환으로 상실된 치주조직의 재생이라고 볼 수 있다. 치주재생술식 후 회복을 평가하는 단계에서, 탐침과정이 초기 회복기의 신생조직에 위해한 영향을 줄 수 있다. 치아동요도는 치주조직이 치아를 지지하고 있는 상태를 평가하는 중요한 지표로 사용된다. 이에 본 연구에서는 치아동요도와 다른 임상지표간의 상관관계를 조사하고, 치아동요도를 측정하는 Periotest$^{(R)}$가 재생술식 후 초기 회복기에 탐침의 필요성을 배제할 수 있는지를 조사하고자 하는 것이다. 치주재생수술을 받은 25명의 환자, 29개의 치아를 대상으로 하였다. 부착수준, 치주낭 심도, 치아동요도 (Periotest$^{(R)}$ value PTV)등의 임상지표를 연구시작단계, 시술 후 6개월, 12개월에 측정하였다. 재생수술 후 모든 임상지표들이 개선되었고 (p<0.05), 각각의 시기에 부착수준, 탐침깊이, 치은퇴축과 PTV 사이의 통계적으로 유의한 상관관계가 관찰되었다. 또한 각 시기 사이의 임상지표와 PTV변화량의 상관관계에서 치주낭깊이와 부착수준이 상관관계가 있는 것으로 관찰되었다. 이상의 결과들로 치주재생술식 이후 임상지표들의 개선을 기대할 수 있으며, 재생술식 이후 회복기에 임상지표의 측정에 PTV가 보조적 역할을 할 수 있을 것으로 사료된다.

• 한국표면공학회:학술대회논문집
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• 한국표면공학회 2017년도 춘계학술대회 논문집
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• pp.134.1-134.1
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• 2017

In this study, we propose a new scaffold fabrication method, "direct electro-hydrodynamic jet process," using the initial jet of an electrospinning process and ethanol media as a target. The fabricated threedimensional (3D) fibrous structure was configured with multilayered microsized struts consisting of randomly entangled micro/nanofibrous architecture, similar to that of native extracellular matrixes. The fabrication of the structure was highly dependent on various processing parameters, such as the surface tension of the target media, and the flow rate and weight fraction of the polymer solution. As a tissue regenerative material, the 3D fibrous scaffold was cultured with preosteoblasts to observe the initial cellular activities in comparison with a solid-freeform fabricated 3D scaffold sharing a similar structural geometry. The cell-culture results showed that the newly developed scaffold provided outstanding microcellular environmental conditions to the seeded cells (about 3.5-fold better initial cell attachment and 2.1-fold better cell proliferation).

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2005년도 제24회 춘계학술대회논문집
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• pp.134-138
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• 2005

재생냉각형 액체로켓 연소기 챔버의 냉각 채널부의 상온상태의 구조설계와 검증시험을 수행하였다. 재생냉각 연소기 냉각 채널부에 사용하는 크롬동 합금의 상온 인장시험을 수행하여 재료의 탄소성 물성치를 확보하였으며 냉각 채널의 탄소성해석은 이 물성치를 이용하여 수행하였다. 해석결과의 검증을 위해서 평판형태의 냉각채널 시편을 제작하여 강도시험을 수행하였다. 탄소성 구조해석과 결과와 시편의 수압시험 데이터를 비교한 결과 구조해석과 시험이 약간의 차이는 있지만 비교적 잘 일치하였으며 채널의 단면 두께가 작기 때문에 제작상의 가공오차가 채널의 구조적인 안정성에 큰 영향을 미치는 것을 확인하였다.

• 한국재료학회지
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• 제24권12호
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• pp.704-709
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• 2014

In this study, we fabricated a novel micro porous hybrid scaffold of biphasic calcium phosphate (BCP) and a polylectrolyte complex (PEC) of chitosan (CS) and hyaluronic acid (HA). The fabrication process included loading of CS-HA PEC in a bare BCP scaffold followed by lypophilization. SEM observation and porosimetry revealed that the scaffold was full of micro and macro pores with total porosity of more than 60 % and pore size in the range of $20{\sim}200{\mu}m$. The composite scaffold was mechanically stronger than the bare BCP scaffold and was significantly stronger than the CS-HA PEC polymer scaffold. Bone morphogenetic growth factor (BMP-2) was immobilized in CS-HA PEC in order to integrate the osteoinductive potentiality required for osteogenesis. The BCP frame, prepared by sponge replica, worked as a physical barrier that prolonged the BMP-2 release significantly. The preliminary biocompatibility data show improved biological performance of the BMP-2 immobilized hybrid scaffold in the presence of rabbit bone marrow stem cells (rBMSC).

• 한국수소및신에너지학회논문집
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• 제26권3호
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• pp.278-286
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• 2015

This paper presents the analytical results of the thermodynamic performance characteristics for a cogeneration system using regenerative organic Rankine cycle (ORC) driven by low-grade heat source. The combined heat and power cogeneration system consists of a regenerative superheated ORC and an additional process heater in a series circuit. Eight working fluids of R134a, R152a, propane, isobutane, butane, R245fa, R123, and isopentane are considered for the analysis. Special attention is paid to the effect of turbine inlet pressure on the system performance such as thermal input, net power and useful heat productions, electrical, thermal, and system efficiencies. The results show a significant effect of the turbine inlet pressure and selection of working fluid on the thermodynamic performance of the system.

• 한국추진공학회지
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• 제8권4호
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• pp.36-42
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• 2004

고성능 액체로켓엔진 연소기 개발을 위해 연소기 챔버 재료로 사용되는 크롬동 소재에 대해서 인장시험을 수행하고 재료 물성치를 확보하였다. 확보한 크롬동 재료 물성치를 이용하여 구조해석을 수행해서 재생냉각형 연소기 시편을 설계하였다. 설계한 시편을 연소기 챔버 제작 공정과 동일한 공정으로 제작 후 강도 시험을 수행하여 시편의 항복압력과 파손 압력을 얻을 수 있었다. 크롬동 소재는 브레이징 후에는 상당한 기계적 물성 저하가 있었고 구조 해석을 통해 예측한 항복 압력이 실제 시험한 결과와 거의 같게 나타났다. 강도 시험 결과에서는 유로 연결부가 유로부보다 먼저 항복과 파손이 일어났다.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2004년도 추계학술대회논문집
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• pp.1067-1071
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• 2004

In this paper, the characteristic of the regenerative pump is reviewed by the measurement and the analysis. The dominant noise sources are harmonic components of the rotating impeller frequency. The acoustic characteristics and the noise source position at the dump are identified. In order to reduce the high-level peak noise, the interior flow of the pump chamber is analyzed by CFD (Computational Fluid Dynamics). Acoustic pressure is calculated with Ffowscs Williams and Hawkings equation. As the result of the analysis new design of the pump chamber is recommended. The recommended pump is compared with original pump by evaluating the RMS value of a interior static pressure and the sound pressure level. The new pump chamber recommended by analysis results is proved by a process of the measurement. The overall SPL of a recommended pump is reduced about 3 dBA.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 1994년도 추계학술대회 논문집
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• pp.49-54
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• 1994

A dynamic model for the cutting process in the end milling process is developed. This model, which describes the dynamic response of the end mill, the chip load geometry including tool runout, the dependence of the cutting forces on the chip load, is used to predict the dynamic cutting force during the end milling process. In order to predict accurately cutting forces and tool vibration, the model, which uses instantaneous specific cutting force, includes both regenerative effect and penetration effect. The model is verified through comparisons of model predicted cutting force with measured cutting forces obtained from machining experiments.

• Molecules and Cells
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• 제43권8호
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• pp.739-748
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• 2020

Stringent regulation of the chondrocyte cell cycle is required for endochondral bone formation. During the longitudinal growth of long bones, mesenchymal stem cells condense and differentiate into chondrocytes. Epiphyseal chondrocytes sequentially differentiate to form growth-plate cartilage, which is subsequently replaced with bone. Although the importance of nuclear factor 1C (Nfic) in hard tissue formation has been extensively studied, knowledge regarding its biological roles and molecular mechanisms in this process remains insufficient. Herein, we demonstrated that Nfic deficiency affects femoral growth-plate formation. Chondrocyte proliferation was downregulated and the number of apoptotic cell was increased in the growth plates of Nfic^-/- mice. Further, the expression of the cell cycle inhibitor p21 was upregulated in the primary chondrocytes of Nfic^-/- mice, whereas that of cyclin D1 was downregulated. Our findings suggest that Nfic may contribute to postnatal chondrocyte proliferation by inhibiting p21 expression and by increasing the stability of cyclin D1 protein.

• International Journal of Stem Cells
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• 제16권1호
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• pp.93-107
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• 2023

Background and Objectives: Chronic periodontitis can lead to alveolar bone resorption and eventually tooth loss. Stem cells from exfoliated deciduous teeth (SHED) are appropriate bone regeneration seed cells. To track the survival, migration, and differentiation of the transplanted SHED, we used super paramagnetic iron oxide particles (SPIO) Molday ION Rhodamine-B (MIRB) to label and monitor the transplanted cells while repairing periodontal bone defects. Methods and Results: We determined an appropriate dose of MIRB for labeling SHED by examining the growth and osteogenic differentiation of labeled SHED. Finally, SHED was labeled with 25 ㎍ Fe/ml MIRB before being transplanted into rats. Magnetic resonance imaging was used to track SHED survival and migration in vivo due to a low-intensity signal artifact caused by MIRB. HE and immunohistochemical analyses revealed that both MIRB-labeled and unlabeled SHED could promote periodontal bone regeneration. The colocalization of hNUC and MIRB demonstrated that SHED transplanted into rats could survive in vivo. Furthermore, some MIRB-positive cells expressed the osteoblast and osteocyte markers OCN and DMP1, respectively. Enzyme-linked immunosorbent assay revealed that SHED could secrete protein factors, such as IGF-1, OCN, ALP, IL-4, VEGF, and bFGF, which promote bone regeneration. Immunofluorescence staining revealed that the transplanted SHED was surrounded by a large number of host-derived Runx2- and Col II-positive cells that played important roles in the bone healing process. Conclusions: SHED could promote periodontal bone regeneration in rats, and the survival of SHED could be tracked in vivo by labeling them with MIRB. SHED are likely to promote bone healing through both direct differentiation and paracrine mechanisms.