• Journal of Periodontal and Implant Science
• /
• 제28권2호
• /
• pp.281-291
• /
• 1998

Chitosan is a biodegradable and non-toxic material with a molecular weight of 800-1,500Kd which can be obtained in various forms with extraordinary chemical structures and biological characteristics of which enables it to be used in many fields as a biomaterial. Ion irradiation is a useful tool to modify chemical structures and physical properties of high molecular weight polymers. The basic hypothesis of this study is that when surface properties of chitosan in a sponge form are modified with ion beam-irradiation and cell adhesion properties of chitosan would improve and thereby increase the regenerative ability of the damaged bone. The purpose of this study was to illuminate the changes in the cytocompatibility of chitosan sponges after ion beam-irradiation as a preliminary research. Argon($Ar^+$) ions were irradiated at doses of $5{\times}10^{13}$, $5{\times}10^{15}$ at 35 keV on surfaces of each sponges. Cell adhesion and activity of alkaline phosphatases were studied using rat fetal osteoblasts. The results of this study show hat ion beam-irradiation at optimal doses($5{\times}10^^{13}\;Ar^+\;ion/cm^2$) is a useful method to improve cytocompatibility without sacrificing cell viability and any changing cell phenotypes. These results show that ion beam-irradiated chitosan sponges can be further applied as carriers in tissue engineering and as bone filling materials.

• Restorative Dentistry and Endodontics
• /
• 제39권1호
• /
• pp.51-55
• /
• 2014

The term "endo-perio" lesion has been proposed to describe the destructive lesion resulting from inflammatory products found in varying degrees in both the periodontium and the pulpal tissues. In most of the cases, clinical symptoms disappear following successful endodontic therapy. However failure after conventional root canal treatment calls for surgical intervention. A 35 year old male patient with endo-perio lesion in right maxillary lateral incisor was treated with platelet rich fibrin (PRF) and alloplastic bone substitute after conventional endodontic therapy. At the end of 6 months there was gain in clinical attachment, increased radiographic bone fill and reduction in probing depth which was maintained till 18 month follow-up. Present case report aims to evaluate the efficacy of PRF and alloplastic bone substitute in the management of intrabony defect associated with endo-perio lesion in maxillary lateral incisor because the healing potential of PRF and bone graft has not been widely studied in endodontics. The use of PRF allows the clinician to optimize tissue remodelling, wound healing and angiogenesis by the local delivery of growth factors and proteins. The novel technique described here enables the clinician to be benefited from the full regenerative capacity of this autologous biologic material.

• 한국조경학회지
• /
• 제28권6호
• /
• pp.29-51
• /
• 2001

The purpose of this research is to analyze of biotope structure focused on the evaluation for the species and biotope preservation in the case of Soosung District in Daegu metropolitan city. We analyzed the structure of urban biotope, and investigated the disposition of space and several characteristics through the material collection relating to the existing urban ecological material and the field investigation. The results of this study were as follows; 1) The result of biotope type classification was divided into 17 biotope type groups and 90 biotope types belonging to them. 2) The biotope type of mixed forest(QD) has the highest rate in occupation area, 1.764ha and the biotope type of cultivation areas by vinyle-hothouse(KC) has the lowest rate, 0.3ha. 3) In the result of the first evaluation for the species and biotope preservation, biotope types such as QB, QI, PA, PB, NB and NC etc. possess great value. Particulary, these biotope types come from forest areas and transition zone. They have not appeared in the inner of cities. 4) The biotope types such as CA, NA, NB, ND and PB are proved to possess high value in the result of rarity and riskiness evaluation. There appear EE, MA, NB, QC and QD in IV grade which is impossible to have them regenerative. They usually have appeared in forest areas. 5) There emerge 3a biotope type that has the highest value in the result of the second evaluation such as CC, EE, KF, LD, MB, NC, PB, QB and QI etc. Most of them were presented into the large area of site size and were distributed in the forest areas intensively. So, it is certain that the space for the species and biotope preservation connected with residential district is scarce. 6) The detailed biotope plan must be made out specially continuously. It is about biotope spaces that are especially important for the species and biotope preservation from the result of this research. Also, the study on the detailed index settlement of the urban landscape plan based on the biotope map must be continued.

• 대한의생명과학회지
• /
• 제19권1호
• /
• pp.41-47
• /
• 2013

Pluripotent stem cells (PSCs) have lots of potential in biomedical sciences owing to its potential to differentiate into any kind of cells in the body. However, it is still a challenge to culture PSCs on a large scale for application to regenerative medicine. Herein, we introduce a synthetic polymer that enables large-scale suspension culture of human PSCs. By employing suspension culture, it became unnecessary to use conventional substrata such as mouse embryonic fibroblast (MEF) or Matrigel$^{TM}$, which are believed to be main causative sources of xenogeneic contamination in cultured human PSCs in vitro. Human PSCs were cultured in the medium in which elastin-like polypeptide (ELP) dissolved. The ELP in the medium became harden as temperature increases by transforming the medium into a semi-solid gel that supported growth of human PSCs in suspension. Gel-sol transition temperature of ELP can be adjusted by modifying the peptide sequence in which 5 amino acids, Val-Pro-Gly-Xaa-Gly, repeated sequentially. We constructed 3D suspension media having transition temperature around $33{\sim}35^{\circ}C$ using an ELP consisted of 40, 60, or 80 repeats of a monomer, which was Val-Pro-Gly-Val-Gly. Among the ELPs, ELP80 was chosen as the best ELP to support growth of human PSCs in suspension culture. This result suggests that the ELP80 can be a medium component for culturing human PSCs in large-scale.

• Archives of Plastic Surgery
• /
• 제49권2호
• /
• pp.266-274
• /
• 2022

Background Many authors have researched ways to optimize fat grafting by looking for a technique that offers safe and long-term fat survival rate. To date, there is no standardized protocol. We designed a "hydraulic system technique" optimizing the relationship among the quantity of injected fat, operative time, and material cost to establish fat volume cutoffs for a single procedure. Methods Thirty-six patients underwent fat grafting surgery and were organized into three groups according to material used: standard, "1-track," and "2-tracks" systems. The amount of harvested and grafted fat as well as material used for each procedure was collected. Operating times were recorded and statistical analysis was performed to establish the relationship with the amount of treated fat. Results In 15 cases the standard system was used (mean treated fat 72 [30-100] mL, mean cost 4.23 ± 0.27 euros), in 11 cases the "1-track" system (mean treated fat 183.3 [120-280] mL, mean cost 7.63 ± 0.6 euros), and in 10 cases the "2-tracks" one (mean treated fat 311[220-550] mL, mean cost 12.47 ± 1 euros). The mean time difference between the standard system and the "1-track" system is statistically significant starting from three fat syringes (90 mL) in 17.66 versus 6.87 minutes. The difference between the "1-track" system and "2-tracks" system becomes statistically significant from 240 mL of fat in 15 minutes ("1-track") versus 9.3 minutes for the "2-tracks" system. Conclusion Data analysis would indicate the use of the standard system, "1-track," and "2-tracks" to treat an amount of fat < 90 mL of fat, 90 ÷ 240 mL of fat, and ≥ 240 mL of fat, respectively.

• Journal of Periodontal and Implant Science
• /
• 제40권1호
• /
• pp.11-18
• /
• 2010

Purpose: Significant interest has emerged in the design of cell scaffolds that incorporate peptide sequences that correspond to known signaling domains in extracellular matrix and bone morphogenetic protein. The purpose of this study was to evaluate the bone regenerative effects of the synthetic peptide in a critical-size rat calvarial defect model. Methods: Eight millimeter diameter standardized, circular, transosseus defects created on the cranium of forty rats were implanted with synthetic peptide, collagen, or both synthetic peptide and collagen. No material was was implanted the control group. The healing of each group was evaluated histologically and histomorphometrically after 2- and 8-week healing intervals. Results: Surgical implantation of the synthetic peptide and collagen resulted in enhanced local bone formation at both 2 and 8 weeks compared to the control group. When the experimental groups were compared to each other, they showed a similar pattern of bone formation. The defect closure and new bone area were significantly different in synthetic peptide and collagen group at 8 weeks. Conclusions: Concerning the advantages of biomaterials, synthetic peptide can be an effective biomaterial for damaged periodontal regeneration.

• Transactions on Electrical and Electronic Materials
• /
• 제17권2호
• /
• pp.57-68
• /
• 2016

In this study, we present the modern hybrid system based power generation for electric vehicle applications. We describe the hybrid structure of modified current source based DC - DC converters used to extract the maximum power from Photovoltaic (PV) and Fuel Cell system. Due to reduced dc-link capacitor requirement and higher reliability, the current source inverters (CSI) better compared to the voltage source based inverter. The novel control strategy includes Distributed Maximum Power Point Tracking (DMPPT) for photovoltaic (PV) and fuel cell power generation system. The proposed DC - DC converters have been analyzed in both buck and boost mode of operation under duty cycle 0.5>d, 0.5<d<1 and 0.5<d for capable electric vehicle applications. The proposed topology benefits include one common DC-AC inverter that interposes the generated power to supply the charge for the sharing of load in a system of hybrid supply with photovoltaic panels and fuel cell PEM. An improved control of Direct Torque and Flux Control (DTFC) based induction motor fed by current source converters for electric vehicle.In order to achieve better performance in terms of speed, power and miles per gallon for the expert, to accepting high regenerative braking current as well as persistent high dynamics driving performance is required. A simulation model for the hybrid power generation system based electric vehicle has been developed by using MATLAB/Simulink. The Direct Torque and Flux Control (DTFC) is planned using Xilinx ISE software tool in addition to a Modelsim 6.3 software tool that is used for simulation purposes. The FPGA based pulse generation is used to control the induction motor for electric vehicle applications. FPGA has been implemented, in order to verify the minimal error between the simulation results of MATLAB/Simulink and experimental results.

• 콘크리트학회논문집
• /
• 제26권5호
• /
• pp.643-650
• /
• 2014

일반적으로, 철근콘크리트 슬래브는 재료의 열적특성에 의해 높은 수준의 화재 저항 성능을 보유하고 있다. 그러나, 중공슬래브의 경우 일반 철근콘크리트 슬래브와는 다른 온도분포 및 화재 저항 성능을 보유하고 있을 것으로 예상된다. 중공슬래브는 콘크리트의 축열 효과를 발생시키는 공기층을 만들어내는 중공을 보유하고 있기 때문이다. 이에 연구에서는 중공슬래브의 화재 저항 성능을 평가하는 방법을 Wickstorm이 제안한 방법을 사용하여 제안하였다. 이 연구에서는 본 연구의 연구자가 제안한 도넛형 중공을 보유한 중공슬래브의 화재저항성능에 대한 단순화된 평가 방안을 제시하였다. 또한, 제안된 방법은 중공슬래브에 대한 화재실험을 통해 검증하였다. 이 연구의 결과를 통해 제안된 방법은 화재 발생 이후 중공슬래브의 잔존강도 추정이 가능함을 확인하였으며, 슬래브에 분포되는 온도의 특성을 정확하게 파악할 수 있는 것을 확인할 수 있었다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• 제41권3호
• /
• pp.119-123
• /
• 2015

Objectives: Dentin is composed of many minerals and growth factors. Based on this composition, we studied its effect as a possible regenerative material for alveolar healing. Materials and Methods: This study was conducted using four 2.5-year-old mongrel dogs (male; weight, 25 to 30 kg). The third mandibular premolars were carefully mobilized with a dental elevator and then removed using forceps. The crown portions of the extracted teeth were removed with cutters, and the root portions of the remaining teeth were collectively trimmed as closely as possible to 350 to $500{\mu}m$. Dentin and cementum (DC) chips harvested from the extracted teeth were soaked in blood and packed into the fresh sockets (autograft). Biopsies were performed at the ends of day 14 and day 56 following implantation. Data were expressed as $mean{\pm}standard$ deviation and compared with t-test results. Results: The ratio of $S_A$(bone) to total area of each probe was determined and was $170{\pm}16{\mu}m^2$ for the control group and $71{\pm}14{\mu}m^2$ for the DC group, a significant difference (P <0.05). Conclusion: DC particulate grafts offered no improvement in bone regeneration in alveolar extraction sockets.

• Journal of Periodontal and Implant Science
• /
• 제25권2호
• /
• pp.341-356
• /
• 1995

The purpose of this study was to evaluate the effect of demineralized freeze dried bone and demineralized bone gel with guided tissue regeneration treatment around titanium implants with dehisced bony defects and also evaluate space maintaining capacity of demineralized bone gel type and DFDB powder type under e-PTFE membrane. In 3 Beagle dogs, mandibular premolar was extracted and four peri-implant osteotomies were formed for dehiscence. After insertion of implants, the four peri-implant defects were treated as follows. 1) In control group. no graft material and barrier membrane were applied. 2) In experimental group.1, the site was covered only with the e-PTFE membrane. 3) In experimental group 2,received DFDB powder and covered by the e-PTFE membrane. 4) In experimental group 3, demineralized bone gel and e-PTFE membrane were used. By random selection, animals were sacrificed at 4, 8, 12 weeks. The block sectioned specimens were prepared for decalcified histologic evaluation(hematoxylin and eosin staining) and undecalcified histologic evahiation(Von Kossa's and toluidine blue staining) with light microscopy. The results of this study were as follows. 1) In control group, there was a little new bone formation and connective tissue was completely filled in the defect area. 2) Experimental group 1 showed lesser quantity of bone formation as compared to the bone grafted group. Thin vertical growth of new bone formation around implant fixture was shown. 3) Experimental group 2 showed thick bucco-lingual growth of new bone formation and grafted bone particles were almost resorbed in 12 week group. 4) In experimental group 3, most grafted bone particles were not resorbed in 12 week group and thick bucco-lingual bone formation was shown in dehisced defect base area. 5) There was no remarkable differences in space making capacity and new bone formation procedure between demineralized freeze-dried bone powder type and demineralized bone gel type.