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Diversity of vir Genes in Plasmodium vivax from Endemic Regions in the Republic of Korea: an Initial Evaluation

  • Son, Ui-han;Dinzouna-Boutamba, Sylvatrie-Danne;Lee, Sanghyun;Yun, Hae Soo;Kim, Jung-Yeon;Joo, So-Young;Jeong, Sookwan;Rhee, Man Hee;Hong, Yeonchul;Chung, Dong-Il;Kwak, Dongmi;Goo, Youn-Kyoung
    • Parasites, Hosts and Diseases
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    • v.55 no.2
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    • pp.149-158
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    • 2017
  • Variant surface antigens (VSAs) encoded by pir families are considered to be the key proteins used by many Plasmodium spp. to escape the host immune system by antigenic variation. This attribute of VSAs is a critical issue in the development of a novel vaccine. In this regard, a population genetic study of vir genes from Plasmodium vivax was performed in the Republic of Korea (ROK). Eighty-five venous blood samples and 4 of the vir genes, namely vir 27, vir 21, vir 12, and vir 4, were selected for study. The number of segregating sites (S), number of haplotypes (H), haplotype diversity (Hd), DNA diversity (${\pi}$ and ${\Theta}_w$), and Tajima's D test value were conducted. Phylogenetic trees of each gene were constructed. The vir 21 (S=143, H=22, Hd=0.827) was the most genetically diverse gene, and the vir 4 (S=6, H=4, Hd=0.556) was the opposite one. Tajima's D values for vir 27 (1.08530, P>0.1), vir 12 (2.89007, P<0.01), and vir 21 (0.40782, P>0.1) were positive, and that of vir 4 (-1.32162, P>0.1) was negative. All phylogenetic trees showed 2 clades with no particular branching according to the geographical differences and cluster. This study is the first survey on the vir genes in ROK, providing information on the genetic level. The sample sequences from vir 4 showed a clear difference to the Sal-1 reference gene sequence, whereas they were very similar to those from Indian isolates.

Motion Estimation and Mode Decision Algorithm for Very Low-complexity H.264/AVC Video Encoder (초저복잡도 H.264 부호기의 움직임 추정 및 모드 결정 알고리즘)

  • Yoo Youngil;Kim Yong Tae;Lee Seung-Jun;Kang Dong Wook;Kim Ki-Doo
    • Journal of Broadcast Engineering
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    • v.10 no.4 s.29
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    • pp.528-539
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    • 2005
  • The H.264 has been adopted as the video codec for various multimedia services such as DMB and next-generation DVD because of its superior coding performance. However, the reference codec of the standard, the joint model (JM) contains quite a few algorithms which are too complex to be used for the resource-constraint embedded environment. This paper introduces very low-complexity H.264 encoding algorithm which is applicable for the embedded environment. The proposed algorithm was realized by restricting some coding tools on the basis that it should not cause too severe degradation of RD-performance and adding a few early termination and bypass conditions during the motion estimation and mode decision process. In case of encoding of 7.5fps QCIF sequence with 64kbpswith the proposed algorithm, the encoder yields worse PSNRs by 0.4 dB than the standard JM, but requires only $15\%$ of computational complexity and lowers the required memory and power consumption drastically. By porting the proposed H.264 codec into the PDA with Intel PXA255 Processor, we verified the feasibility of the H.264 based MMS(Multimedia Messaging Service) on PDA.

Comparative assessment of age, growth and food habit of the black-chinned tilapia, Sarotherodon melanotheron (Rüppell, 1852), from a closed and open lagoon, Ghana

  • Zuh, Cephas Kwesi;Abobi, Seth Mensah;Campion, Benjamin Betey
    • Fisheries and Aquatic Sciences
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    • v.22 no.12
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    • pp.31.1-31.12
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    • 2019
  • Background: The black-chinned tilapia, Sarotherodon melanotheron, is the most abundant fish species in the Nakwa (an open lagoon) and Brenu (a closed lagoon) in the Central Region of Ghana. Aspects of the life history characteristics and the ecology of the fish populations in both lagoons were studied to assess the bio-ecological status of this important resource. Methods: Fish samples were obtained from fishermen that fish on the Nakwa and Brenu lagoons using cast, drag and gill nets. The age of the fish was assessed from otoliths analysis and its growth modelled following the von Bertalanffy growth function. Morphometric characteristics of the fish populations were analysed using power regression and ANOVA for parameters comparisons, and Student's t test to determine whether species grew isometrically. The percentage occurrence method was used to analyse the stomach contents of the fish. Results: A total of 382 fish samples from both lagoons were measured, comprising 209 from Nakwa lagoon and 176 from Brenu lagoon. The size and weight of fish samples ranged between 3.9-11.5 cm total length and 1.0-27.3 g for Nakwa Lagoon and 5.6-12.8 cm total length and 3.2-29.8 g for the Brenu Lagoon. The estimated von Bertalanffy growth parameters were L∞ = 12.04 cm and K = 2.76/year for the Nakwa Lagoon samples and L∞ = 13.44 cm and K = 3.27/year for Brenu Lagoon samples. Daily otolith incremental rate ranged from 0.01-0.03 mm per day to 0.01-0.02 mm per day for Nakwa and Brenu lagoons, respectively. Stomach content analysis of the fish samples revealed that the species are planktivorous and the range of food varied between the lagoons. Green algae were the most prevalent food item in the stomachs of the fish samples from Nakwa with the frequency of 69% whilst diatoms (80.5%) were most prevalent phytoplanktonic food item for the fish in Brenu lagoon. Conclusions: The estimates of asymptotic length for the species in both lagoons are close to known values of the species length at first sexual maturity and points to intensive fishing pressure. As a consequence, a comprehensive sample-based survey is required in both lagoons to derive estimates of management reference points. The results of the stomach content analysis are beneficial to the construction of diet matrix for ecosystem models of the two systems.

Genome-wide Copy Number Variation in a Korean Native Chicken Breed (한국 토종닭의 전장 유전체 복제수변이(CNV) 발굴)

  • Cho, Eun-Seok;Chung, Won-Hyong;Choi, Jung-Woo;Jang, Hyun-Jun;Park, Mi-Na;Kim, Namshin;Kim, Tae-Hun;Lee, Kyung-Tai
    • Korean Journal of Poultry Science
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    • v.41 no.4
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    • pp.305-311
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    • 2014
  • Copy number variation (CNV) is a form of structural variation that shows various numbers of copies in segments of the DNA. It has been shown to account for phenotypic variations in human diseases and agricultural production traits. Currently, most of chicken breeds in the poultry industry are based on European-origin breeds that have been mostly provided from several international breeding companies. Therefore, National Institute of Animal Science, RDA has been trying to restore and improve Korean native chicken breeds (12 lines of 5 breeds) for about 20 years. Thanks to the recent advance of sequencing technologies, genome-wide CNV can be accessed in the higher resolution throughout the genome of species of interest. However, there is no systematic study available to dissect the CNV in the native chicken breed in Korea. Here, we report genome-wide copy number variations identified from a genome of Korean native chicken (Line L) by comparing between the chicken reference sequence assembly (Gallus gallus) and a de novo sequencing assembly of the Korean native chicken (Line L). Throughout all twenty eight chicken autosomes, we identified a total of 501 CNVs; defined as gain and loss of duplication and deletion respectively. Furthermore, we performed gene ontology (GO) analysis for the putative CNVs using DAVID, leading to 68 GO terms clustered independently. Of the clustered GO terms, genes related to transcription and gene regulation were mainly detected. This study provides useful genomic resource to investigate potential biological implications of CNVs with traits of interest in the Korean native chicken.

Analysis of the Factors Influencing the Mesopore Ratio on the Soil Surface to Investigate the Site Factors in a Forest Stand(I) - With a Special Reference to Coniferous Stands - (산림(山林)의 입지환경인자(立地環境因子)가 표층토양(表層土壤)의 조공극률(粗孔極律)에 미치는 영향인자(影響因子) 분석(分析)(I) - 침엽수림(針葉樹林)을 중심(中心)으로 -)

  • Jeong, Yongho;Park, Jae Hyeon;Kim, Kyong Ha;Youn, Ho Joong
    • Journal of Korean Society of Forest Science
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    • v.90 no.3
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    • pp.314-323
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    • 2001
  • This study aimed to clarify the influencing factors of mesopore ratio on a pore geometry of surface soil in coniferous stands as an index of the water retention capacity. Twenty three factors including site conditions and soil properties were analyzed by spss/pc + for the data collected during March to October of 1993. The factors influencing the mesopore ratio(pF2.7) on the surface soil were as follows; macropore ratio(pF1.6), slope, crown-cover rates, thickness of F layer, organic matter contents, and the growing stock. And influencing factor on the ratio of mesopore in the soil surface was correlated with percentage of amount of clay, soil surface, A and B horizon soil hardness shows high negative significance. Also, multiple regression equations for mesopore ratios of surface soil and surface soil hardness, clear length, growing stock, B horizon of soil hardness, organic matter contents show high significance($R^2$; 0.80). In coniferous stands, it is effective in promoting development on the ratio of mesopore that forest practice for enhancing of the water resource retention capacity should be carried out when the crown-cover rates of stands are more than 80 percentages.

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A Study on Economic Evaluation of Beneficiary Pays Principle in Water Resource Management - The Case of Namyangju in Korea - (수자원관리 부문에서 수혜자부담원칙 경제적 평가에 관한 연구 - 남양주시 사례분석 -)

  • Yoon, Jaehyun
    • Journal of Environmental Impact Assessment
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    • v.23 no.5
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    • pp.323-336
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    • 2014
  • Using hedonic price method, this paper analyzes the impact of restriction for water quality protection on property value with the officially announced price of reference land in the city of Namyangju in 2012 to evaluate Water Use Fee, based on beneficiary pays principle, levied on the downstream area of the Han River in Korea. The results from the regression analyses of the models used show that the double-log model is the preferred model in the case of Namyangju. Using the double-log model, the total compensation for the city of Namyangju is estimated to be 8.6 trillion won with 95% confidence interval between 4.4 trillion and 12.4 trillion won. Under the perpetuity compensation scheme at the discount rate of 10%, the estimated annual compensation is 0.9 trillion won with 95% confidence interval between 0.4 trillion and 1.2 trillion won. This is more than Water Use Fee collected in 2012 for the Han River, which is approximately 0.5 trillion won. Considering the size of the restricted area of the Paldang area, which is more than 18 times of that of Namyangju, the rate of Water Use Fee, which is based on beneficiary pays principle and imposed on the residents of the downstream area, needs to be increased to sufficiently compensate the economic loss caused to the upstream areas of the Han River in Korea.

Study on Surface Temperature Change of PV Module Installed on Green Roof System and Non-green Roof System (옥상녹화와 비 옥상녹화 평지붕에 설치 된 PV모듈의 표면온도 변화 고찰)

  • Yoo, Dong-Chul;Lee, Eung-Jik;Lee, Doo-Ho
    • 한국태양에너지학회:학술대회논문집
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    • 2011.11a
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    • pp.214-219
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    • 2011
  • Today, various activities to save energy are being conducted around the world. Even in our country, carbon reduction policy is being conducted for low carbon green growth and with this movement, effort to replace energy sources by recognizing the problems on environment pollution and resource exhaustion due to the indiscrete usage of fossil fuel is being made. Therefore, active study on renewable energy is in progress as part of effort to replace the energy supply through fossil fuel and solar ray industry has rapidly developed receiving big strength of renewable energy policies. The conclusion of this study measuring the surface temperature change of single crystal and polycrystalline PV module in green roof system and non-green roof system aspect are as follows. There was approximately $4^{\circ}C$ difference in PV module temperature in green roof system and non-green roof system aspect and this has the characteristic to decrease 0.5% when the temperature rises by $1^{\circ}C$ when the front side of the module is $20^{\circ}C$ higher than the surrounding air temperature following the characteristic of solar cells. It can be concluded that PV efficiency will be come better when it is $4^{\circ}C$ lower. Also, in result of temperature measurement of the module back side, there was $5^{\circ}C$ difference of PV module installed on the PV module back side and green roof system side on the 5th, $3^{\circ}C$ on the 4th, $2^{\circ}C$ on the 5th to show decreasing temperature difference as the air temperature dropped, but is judged that there will be higher temperature difference due to the evapotranspiration latent heat effect of green roof system floor side as the temperature rises. Based on this data, it is intended to be used as basic reference to maximize efficiency by applying green roof system and PV system when building non-green roof system flat roof.

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Comparative Study on the Impact Factors in Job Stress in Occupational Therapists Working in Korean: A Systematic Review and Meta-Analysis (국내 작업치료사의 직무스트레스 요인 비교 연구: 체계적 고찰과 메타분석)

  • Cha, Yu-Jin
    • The Journal of the Korea Contents Association
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    • v.12 no.6
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    • pp.380-389
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    • 2012
  • In this study, Systematic review and meta-analysis using the correlation coefficients was carried out to integrate precedent studies on factors affecting domestic occupational therapists' job stress. It aims at providing basic information resources of preventing and reducing stress of occupational therapists and effective counter-measurement to improve quality of occupational therapy and to establish efficient human resource management policy. Systematic review and meta-analysis were performed on eight thesis proven relevant to selection criteria in order to figure out correlation coefficients value by total, and factors correlation coefficients value. Also homogeneity test and publication bias test was performed too. The total correlation coefficients value of occupational therapists was .30 which was statistically significant. As to job stress factors, the organization related factor showed the highest correlation of coefficiency, followed by factors other than the organization related, physical environment, job related factor and personal factor. This research result can be used as a reference to prevent and reduce job stress of occupational therapists and to develop an effective measurement scheme.

Three dimensional cone-beam CT study of upper airway change after mandibular setback surgery for skeletal Class III malocclusion patients (Cone-beam CT를 이용한 골격성 III급 부정교합자의 하악골 후퇴술 후 상기도 변화에 관한 연구)

  • Kim, Na-Ri;Kim, Yong-Il;Park, Soo-Byung;Hwang, Dae-Seok
    • The korean journal of orthodontics
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    • v.40 no.3
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    • pp.145-155
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    • 2010
  • Objective: Lateral cephalometric radiographs have been the main form of resource for assessing two dimensional anteroposterior airway changes. The purpose of this study was to evaluate the three dimensional volumetric change in the upper airway space in Class III malocclusion patients who underwent mandibular setback surgery. Methods: Three dimensional cone-beam computed tomographs (CBCT) and their three dimensional reconstruction images were analyzed. The samples consisted of 20 adult patients (12 males and 8 females) who were diagnosed as skeletal Class III and underwent mandibular setback surgery. CBCTs were taken at 3 stages - Baseline (1.8 weeks before surgery), T1 (2.3 months after surgery), and T2 (1 year after surgery). Pharyngeal airway was separated according to the reference planes and reconstructed into the nasopharynx, the oropharynx and the hypopharynx. Measurements at Baseline, T1, and T2 were compared between groups. Results: The result showed the volume of the pharyngeal airway decreased significantly 2.3 months after surgery (p < 0.001) and the diminished airway did not recover after 1 year post-surgery. The oropharynx was the most decreased area. Conclusions: These findings suggest that mandibular setback surgery causes both short-term and long-term decrease in the upper airway space.

The Uneven Distribution of Mating Type Genes in Natural and Cultivated Truffle Orchards Contributes to the Fructification of Tuber indicum

  • Li, Qiang;Fu, Yu;Sun, Qun;Sourzat, Pierre;Yang, Mei;Liu, Chengyi;Tan, Hao;Ye, Lei;Zou, Jie;Wu, Chenguang;Zhang, Bo;Li, Xiaolin
    • Mycobiology
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    • v.46 no.1
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    • pp.64-71
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    • 2018
  • The aim of this study was to investigate the pattern of distribution of mating type (MAT) genes of Tuber indicum in ectomycorhizosphere soils from natural T. indicum-producing areas and cultivated truffle orchards and ascocarp samples from different regions. Quantitative real-time PCR and multiplex PCR were used to weight the copy numbers of MAT1-1-1 and MAT1-2-1 in natural truffle soils and cultivated orchard soils. The effect of limestone on the pattern of truffle MAT genes and the correlation between soil properties and the proportion of MAT genes were also assessed. These results indicated that an uneven and nonrandom distribution of MAT genes was common in truffle-producing areas, cultivated truffle orchards, and ascocarps gleba. The competition between the two mating type genes and the expansion of unbalanced distribution was found to be closely related to truffle fructification. Limestone treatments failed to alter the proportion of the two mating type genes in the soil. The content of available phosphorus in soil was significantly correlated with the value of MAT1-1-1/MAT1-2-1 in cultivated and natural ectomycorhizosphere soils. The application of real-time quantitative PCR can provide reference for monitoring the dynamic changes of mating type genes in soil. This study investigates the distributional pattern of T. indicum MAT genes in the ectomycorhizosphere soil and ascocarp gleba from different regions, which may provide a foundation for the cultivation of T. indicum.