• Korean Journal of Plant Taxonomy
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• v.49 no.4
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• pp.374-379
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• 2019

In this study, we report a new record of the clustered bur-reed Sparganium glomeratum (Laest. ex Beurl.) Beurl., from Yongneup Moor of Daeamsan Mt. in Gangwon-do, Korea. This species is distributed in the cool temperate and circumboreal regions of the Northern Hemisphere. As we recorded this species in a high-altitude wetland, we named it "Du-me-heuk-sam-neung" after the Korean word that translates to "Sparganium found in deep mountains." We provide descriptions of the morphological characteristics, photographs, and detailed illustrations of S. glomeratum, as well as a key to allied taxa in Korea.

• Journal of The Korean Society of Grassland and Forage Science
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• v.6 no.1
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• pp.24-30
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• 1986

This experiment was carried out in order to study the effect of fertilizer application and seasonal changes on the productivity of the native reed (Phragmites communis Trinius) on the reclaimed tidal flate in Chonnam province. The samples of reed were taken at about 30-days interval from May to October, 1982 and evaluated plant height, blade & sheath-stem ratio, grass yield, the feed compositions and in vitro dry matter digestibility (IVD). The results are summarized as follows: 1. Plant height, grass yield, crude protein content and in vitro dry matter digestibility of the reed were significantly increased by fertilizer application, and ADF content was significantly decreased. 2. According to the significance test of coefficience, there was a significant negative correlation (P<0.05) between in vitro dry matter digestibility and plant height, grass yield, and the content of crude fiber and ADF, but a positive correlation (P<0.05) between in vitro dry matter digestibility and blade & sheath-stem ratio, and the content of crude protein and crude fat.

• Journal of Wetlands Research
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• v.15 no.1
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• pp.35-41
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• 2013

In this study, we tried to overcome a limit in cutting timing of reed culm by applying a plant hormone, indole-acetic acid (IAA) as a growth regulator with various contents ($10^{-3}$ M, $10^{-6}$ M, $10^{-9}$ M, $10^{-12}$ M). 19 shoots emerged from 240 segments of hardened reed culm from montane fen and eight out of the 19 shoots emerged by $10^{-6}$ M IAA treatment as the most in $5^{th}$ Sep. 2012. 50 shoots emerged from 60 segments of non-hardened reed culm from a population in Seoul National University transplanted from Mt. Odae by $10^{-6}$ M IAA treatment despite the cutting was performed about two weeks later ($19^{th}$ Sep.). Via third cutting experiment performed about 40 days later ($29^{th}$ Oct.), only two shoots out of 60 segments were observed by the same experimental condition except atmospheric temperature. It seemed likely that it was too low temperature in third experiment ($10^{\circ}C$) than the former experiments (about $20^{\circ}C$) to form adventitious buds from culm segment. We recommend to utilize the thick reed culm in culm cutting as possible because the thicker culm segment we used, the thicker emerged shoot we could observe (i.e., diameters of emerged shoots were about 20% of the planted segment's diameters).

• Korean Journal of Organic Agriculture
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• v.6 no.1
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• pp.51-61
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• 1997

This experiment was carried out to investigate the growth response of 3 grasses to seed coating with chitosan solution and the attempt was made to estimate adequate seed coating concentrations of chitosan solution in each grass for the growth to be stimulated. Three species used in this experiment were orchardgrass, tall fescue and reed canarygrass. Six different seed coating concentrations of chitosan solution were applied as 0%(control), 0.01%, 0.05%, 0.1% and 1.0%, respectively. the results were obtained as follows; 1. Dry weight of tiller(WT), leaf area(LA), dry weight of leaf(LW), dry weight of stem(SW), dry weight of shoot(SHW), biological yield(BY) and C/F ratio were significantly different between species. 2. Number of tillers per plant(NT), dry weight of tiller(WT), dry weight of leaf(LW), dry weight of root(RW), dry weight of shoot(SHW), biological yield(BY) and T/R ration were significantly different between seed coating concentrations of chitosan solution. 3. The adequate seed coating concentrations of chitosan solution for the growth stimulating effect were different between species. The highest values of yield components and dry weight of plant parts were obtained at 1% in orchardgrass and tall fescue, and 0.05% in reed canarygrass, respectively. 4. Growth stimulating effect of seed coating in each species were different. The highest values were obtained in leaf area(LA), dry weight of leaf(LW), dry weight of root(RW), dry weight of shoot(SHW) and dry weight of biological yield(BY) in orchardgrass. The values of dry weight of stem(SW) and C/F ration were highest in reed canarygrass. 5. An increase in number of tillers per plant(NT), dry weight of leaf(LW), dry weight of stem(SW) and dry weight of root(RW) according to seed coating was attributed to the increase in dry weight of shoot(SHW). Among the aboved increasing factors, the dry weight of leaf(LW) was a main factor for the increase in dry weight of shoot(SHW). 6. An increase in dry weight of leaf(LW), dry weight of stem(SW) and dry weight of root(RW) according to seed coating was attributed to the increase in biological yield(BY). Both the dry weight of leaf(LW) and dry weight of root(RW) were main factors for the increase in biological yield(BY).

• Journal of The Korean Society of Grassland and Forage Science
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• v.20 no.1
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• pp.55-60
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• 2000

This experiment was carried out to determine the effect of cutting frequency(3rd and 5th cut) and level of nitrogen fertilization(l50 + liquid manure, 300 and 450kgha) on growth characteristics, dry matter yield and nutritive vaule of reed canarygrass(Pha1aris arundinacea L.), at the forage experimental field, College of Agriculture and Life Sciences, SNU, Suweon. Plant height of 3rd cutting(79.2cm) was significantly higher than that of 5th cutting(58.0cm). And dry mattter yield of cutting frequency was observed in 3rd cutting(16,902kgPna) and 5th cutting(l7,833kg/ha) As the nitrogen fertilization was increased, DM yield was significantly increased(p<0.05). When the nitrogen fertilization was applied at the level 150 + liquid manure, 300 and 450kg, dry matter yields were 16,5 12, 17,123 and 18,368kg/ha, respectively. The contents of neutral detergent fiber(NDF), acid detergent fiber(ADF) and in vitro dry matter digestibility(1VDMD) were similar among cutting frequency. However, slightly higher NDF, ADF and IVDMD contents were found in 5th cutting, but there was not affected by nitrigen fertilization. Based on the results of this experiment, it were indicated that to 5th cutting and 450kg nitrogen fertilization could be obtained from the increase of dry matter yield and nutritive value (Key words : Cutting frequency, Nitrogen fertilization, Dry matter yield, Nutritive Value, Reed canarygrass)

• Journal of Soil and Groundwater Environment
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• v.20 no.6
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• pp.73-84
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• 2015

Nine plant species were selected through vegetation survey at three military shooting ranges at northern Gyeonggi Province. Plants were germinated in normal soil and three seedlings were transplanted to a bottom sealed pot containing sandy loam soils contaminated with either RDX (291 mg/kg) or TNT (207 mg/kg). Planted, blank (without plant), and control (without explosive compound) pots were grown in triplicate at a green house for 134 days. During cultivation, transplanted plants exhibited chlorosis and necrosis in flower and leaf by explosive toxicity and stress. Only three plants, Wild soybean, Amur silver grass, Reed canary grass, survived in TNT treated pot, while seven plant species except for field penny cress and jimson weed, thrived in RDX treated pot. Appreciable amount of TNT (61.6~241.2 mg/g-D.W.) was detected only in plant roots. Up to 763.3 mg/g-D.W. along with 4-amino-2,6-dinitrotoluene, an intermediate of TNT, accumulated in the root of wild soybean. In addition, azoxy compounds, abiotic intermediates of TNT, were detected in TNT treated soils. RDX absorbed average 1,839.95 mg/kg in shoot and 204.83 mg/kg in root. Most of TNT in plant was accumulated in underground part whereas RDX was localized in aerial part. Material balance calculation showed that more than 95% of the initial TNT was removed in the planted pots whereas only 60% was removed in the blank pot. The amount of RDX removed from soil was in the order of Amur Silver Grass (51%) > Chickweed (43%) > Evening primrose (38%). Based on the results of pot cultures, Amur silver grass and Reed canary grass are selected as tolerant remedial plants for explosive toxicity.

• Journal of The Korean Society of Grassland and Forage Science
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• v.15 no.2
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• pp.112-117
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• 1995

A field experiment was canied out to determine the effects of harvest time (grazing, soiling and hay stage) on the grass growth, dry matter (DM) yield, nutritive value, and weed development in reed canarygrass (Phalaris arundinacea L.) pasture. The cultivars of reed canarygrass used in this study were Palaton, Venture, Castor, and Frontier (control). The grass was harvested 3 times at hay stage, 4 times at soiling stage, and 6 times at grazing stage in 1992. The average plant height was 31-41cm at grazing, 47-59cm at soiling, and 76-86cm at hay stage. Similar DM yield was observed in Palaton(12,567 kglha), Venture(l2,435kg), and Frontier(12,762kg), and the yields of 3 cultivars were significantly higher than that of Castor(ll,025kg) (P<0.05). Total yields affected by harvest time were lO,O6 1, 1 1,832, and 14,699 kg/ha at grazing, soiling, and hay stage, respectively(P<0.05). The content of crude protein (CP) was not significantly different among cultivars. CP contents by harvest time were 21.6% at grazing, 19.3% at soiling, and 18.8% at hay stage, regardless of cultivm(P<0.05). The contents of neutral detergent fiber (NDF) and acid detergent fiber (ADF) were similar among cultivars and harvest time. However, slightly higher NDF and ADF contents were found in Frontier. The contents of Ca, P, K, and Mg were not affected by cultivars and harvest time. The appearance of weeds was high in Castor, and low in Frontier. The weeds at grazing stage(33.3-60.0%) were remarkably higher than those at soiling and hay astage (ca 10%). In conclusion, it is suggested that 3-4 times (soiling and hay stage) of harvest was recommended for grass production, nutritive value, and weed control in reed canarygrass pasture, regradless of cultivars of Palation, Venture and Frontier, except Castor.

• Journal of Korean Society of Environmental Engineers
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• v.36 no.4
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• pp.237-245
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• 2014

Amur silver grass (Miscanthus sacchariflorus) and reed canary grass (Phalaris arundinacea) were selected for RDX removal experiments in hydroponic culture conditions based on vegetation survey at three shooting ranges in northern Kyunggi province. Seedling of two plants were grown in 1/4 strength Hoagland solution in quadruplicates containing 10, 20, 30, 40 mg/L RDX for 15 days along with control and blank treatments. During the 15 days of incubation, pH and RDX concentration in medium were routinely analyzed and RDX contents in the shoot and the root were determined after solvent extraction at the end of the experiments. Both plant species showed no symptoms of RDX phyto-toxicity. The pseudo first order RDX-removal constants for amur silver grass and reed canary grass were in the range of $0.0143{\sim}0.0484day^{-1}$ and $0.0971{\sim}0.1853^{-1}$, respectively. Plant biomass normalized RDX removal rates, which decreased with the increase of initial RDX concentration, were in the range of $0.27{\sim}1.01mL{\cdot}g^{-1}day^{-1}$ and $0.87{\sim}1.66mL{\cdot}g^{-1}day^{-1}$ for amur silver grass and reed canary grass, respectively. After 15 days of treatment, RDX removal from the medium decreased from 49.0% to 23.7% with increase in the initial RDX concentration in amur silver grass and 7.3% of the initial RDX remained in the plant. In reed canary grass planted medium, less than 16.8% and 5% of the initial RDX remained in the medium and in the plant, respectively. Large quantities of unidentified polar compound, which was not detected in amur silver grass, accumulated in the root and shoot of reed silver grass.

• Journal of Plant Biotechnology
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• v.47 no.4
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• pp.324-329
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• 2020

Reed (Phragmites spp.) is a rhizomatous plant of the Poaceae family and is known as high tolerant plant to heavy metal contaminants. This plant is widely recognized as a Cd root-accumulator, but improved heavy metal tolerance and uptake capacity are still required for phytoremediation efficiency. To enhance capacity of hyperaccumulator plants, ethyl methane sulfonate (EMS) as chemical mutagen has been introduced and applied to remediation approaches. This study aimed to select EMS-mutagenized reeds representing high Cd resistance and large biomass and to investigate their ability of Cd accumulation. After 6 months cultivation of M₂ mutant reeds under Cd stress conditions (up to 1,500 µM), we discovered seven mutant individuals that showed good performances like survivorship, vitality, and high accumulation of Cd, particularly in their roots. Compared to wild type (WT) reeds as control, on average, dry weight of mutant type (MT) reeds was larger by 2 and 1.5 times in roots and shoots, respectively. In addition, these mutant plants accumulated 6 times more Cd, mostly in the roots. In particular, MT8 reeds showed the greatest ability to accumulate Cd. These results suggest that EMS mutagenesis could generate hyperaccumulator plants with enhanced Cd tolerance and biomass, thereby contributing to improvement of phytoremediation efficiency in Cd-contaminated soil or wastewater. Further studies should focus on identifying Cd tolerance mechanisms of such EMS-mutagenized plants, developing techniques for its biomass production, and investigating the practical potential of the EMS mutants for phytoremediation.

• Microbiology and Biotechnology Letters
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• v.38 no.3
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• pp.302-307
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• 2010

Nitrogen is an element need to grow plants growth. Plants take up nitrogen in the form of nitrate or ammonium. Most of plants absorb nitrogen source as fertilizers. But from 50 to 70% of fertilizers applied were washed away. This study was conducted to isolate free-living nitrogen fixing bacteria from reed and to examine its beneficial traits for developing sustainable biofertilizers. Enriched consortium obtained from a reed in Ansan was developed for the fixing of nitrogen. Nitrogen fixing bacteria isolated from an enriched culture in Congo Red Medium was analyzed by 16s rDNA sequencing. AKC-10 was isolated and shown to have excellent nitrogen fixing ability. The optimum conditions of nitrogen fixing ability were $25^{\circ}C$ ($237.50{\pm}39.65\;nmole{\cdot}mg-protein^{-1}{\cdot}h^{-1}$ and pH 7 ($168.335{\pm}12.84$ nmole/hr mg-protein). It was identified as Microbacterium hominis [(AKC-10 (similarity : 99%)]. This strain was had to IAA (indole-3-acetic acid) productivity and ACC(1-aminocyclopropane-1-carboxylic acid) deaminase activity. Therefore, Microbacterium hominis AKC-10 stimulated plant development in the soil, enhancing the efficiency of remediation.