• 한국잡초학회지
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• 제17권1호
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• pp.24-30
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• 1997

담수 직파 벼에서 최근 많이 사용하고 있는 제초제들의 영양(營養) 유무(有無)에 따른 약해 반응을 검토한 결과는 다음과 같다. 1. Bensulfuron-methyl, pyrazosulfuron-ethyl 및 imazosulfuron의 처리는 경엽부(莖葉部)보다 근부(根部)의 생육을 크게 억제시켰으며 영양(營養) 조건 재배에서 무영양(無營養) 조건의 재배에서보다 생육 억제 정도가 컸다. 또한 영양(營養) 재배와 무영양(無營養) 재배에서 벼 생육 억제율(抑制率)의 차이는 pyrazosulfuronethyl의 처리에서 가장 컸다. 2. Dimepiperate의 처리는 경엽부(莖葉部) 근부(根部) 모두 무영양(無營養) 조건에서 생육 저해 정도가 컸으며, 근부(根部)보다 경엽부(莖葉部)의 생육을 크게 억제시켰으며, 특히 초장신장이 크게 억제되었다. 3. Molinate 처리는 경엽부(莖葉部)의 억제율(抑制率)이 근부(根部)보다 컸으며 경엽부(莖葉部)는 영양(營養)조건에서, 근부(根部)는 무영양(無營養) 조건에서 생육 억제율(抑制率)이 큰 경향이었다.

• 한국축산식품학회지
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• 제34권3호
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• pp.346-354
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• 2014

Fermented foods with probiotics having functional properties may provide beneficial effects on health. These effects are varied, depending on the type of lactic acid bacteria (LAB). Different probiotic LAB might have different functional properties. Thus, this study was performed to evaluate the quality of fermented sausages manufactured with functional starter cultures (Lactobacillus plantarum 115 and 167, and Pediococcus damnosus L12) and different fat levels, and to determine the optimum condition for the manufacture of these products. Medium-fat (~15%) fermented sausages reduced the drying time and cholesterol contents, as compared to regular-fat counterparts. In proximate analysis, the contents of moisture and protein of regular-fat products were lower than medium-fat with reduced fat content. The regular-fat products also had a lighter color and less redness, due to reduced fat content. Approximately 35 volatile compounds were identified in functional fermented sausages, and hexanal, trans-caryophyllene, and tetradecanal were the major volatile compounds. Selected mixed starter culture showed the potential possibility of replacing the commercial starter culture (LK30 plus) in flavor profiles. However, medium-fat fermented sausage containing selected mixed starter culture tended to be less acceptable than their high-fat counterparts, due to excess dry ring developed in the surface. These results indicate that the use of combinations of L. plantarum 115 and 167, and P. damnosus L12 as a starter culture, will prove useful for manufacturing the fermented sausage.

• 대한미생물학회지
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• 제11권1호
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• pp.79-85
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• 1976

There are many of anaerobic culture methods and equipments for isolation and cultivation of anaerabic bacteria, but most of these methods are used without pre-reduced media. Gas-jet method is a recommend. able method for the culture of anaerobes, resently developed. Bacteriological studies were experimented of liver abscess of cattle by the use of gas. jet method. The results were summarised as follows; 1. Gas-jet method for anaerobic culture are expedient for the making of pre-reduced media, maintaining of oxygen free condition in the culture tube, picking of bacteria from colony and colony counting etc. 2. A 121 strains of facultative anaerobic and anaerobic bacteria were isolated from liver abscess of 27 head of cattle, and the isolated anaerobic bacteria were as follows. Peptostreptococcus spp. 7 strains Acid aminococcus fermentans 1 Veillonella spp. 1 Bacterioides spp. 6 Bifidobacterium spp. 4 Arachinia propionica 2 Lactobacillus spp. 4 Propionibacterium acnes 1 3. Liver abscess were infected with many of bacteria, about $10^3-10^9$ numbers per gram of abcessed tissue. Almost of abscess were mixed infection of various bacterial species rather than simple species.

• Journal of Oral Medicine and Pain
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• 제24권2호
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• pp.163-169
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• 1999

We investigated the culture condition and effects of various growth factors on the culture of salivary gland acinar cells. Male, Sprague-Dawley rats (about 6 weeks old) were sacrificed and their submandibular, sublingual, and parotid glands were used as specimens. High oxygen level more than 90% and coating of Matrigel on culture dish were important factors to help increase the survival time of acinar cells, Proper concentration of enzymes such as collagenase and hyaluronidase during isolation steps was also important. Addition of various growth factors such as dexamethasone, insulin, transferrin, selenous acid, reduced glutathione, epidermal growth factor, isoproterenol, and putrescine in culture medium helped to increase lifetime of cultured salivary acinar cells.

• 미생물학회지
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• 제40권3호
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• pp.217-220
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• 2004

Cordyceps militaris의 액체 배양시 cordycepin(3'-deoxyadenosine) 생성에 영향을 주는 물리화학적 조건 및 배지 조성의 영향을 조사하였다. 사용된 17종 균주 중에서 C. militaris KCTC 6862, C. militaris KCTC 16932 및 C. militaris DGUM 32003이 우수한 cordycepin생산성을 보여주었다. Cordycepin생산에 적합한 최적 온도와 PH는 각각 $24^{\circ}C$와 pH 6.0-10범위이었다. YM배지에 탄소원으로 glucose를, 질소원으로 tryptone을 각 1% 첨가하였을 매 우수한 cordycepin 생산을 보여주었다. Tryptone을 질소원으로 하는 YMG 액체배지에서 5일간 배양하였을 경우, 39mg/l의 cordycepin의 농도가 측정되었다. 0.1%의 다양한 인산원을 각각 첨가한 결과, cordycepin생산을 저해하였다.

• 한국작물학회지
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• 제48권3호
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• pp.160-168
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• 2003

본 연구에서 간편 고효율 습해 저항성 검정법을 개발하기 위한 연구의 일환으로 동계습해와 춘계습해의 주요 유발요인의 조절이 용이한 수경재배 조건을 이용하여 산소, 양분 및 유기산의 농도가 보리 유묘의 생육에 미치는 영향을 조사한 결과는 다음과 같다. 1. 배양액의 질소가스 통기처리에 의해 유도되는 혐기조건이 보리 유묘의 생육을 저하시키며 생육감소정도는 근장 그리고 근생체중 순으로 크게 나타났다. 2. 조사시기에 따라 양분 결집 처리에 따른각 조사형질의 반응이 다르나. 지상부 길이와 생체중이 양분결핍처리에 의해 감소하는 경향이었다. 양분결핍조건에 대한 반응은 지상부 형질이 지하부 형질보다 더 민감한 경향이였으나, 그 감소 정도는 크지 않았다. 3. 보리의 생육은 CA 처리에 의해 감소하였으며 CA 농도 가 높을수록, 처리기간이 길수록 감소정도가 컸다. 4. 생육은 혐기 및 양분결핍복합처리 조건에서 감소하였는데, 그 경향은 처리 7일 후에 가장 뚜렷하였으며 처리일수가 경과할수록 복합처리효과는 감소하는 경향이었다. 혐기 및 양분결핍처리에 의한 생육감소정도가 가장 큰 형질은 근장이었다. 5. 혐기와 양분결핍복합처리에서 생육저하효과는 혐기처리 효과가 양분처리효과보다 큰 경향이었다. 혐기와 양분결핍처리의 상호작용효과는 처리 14일 근생체중과 근건물중에서만 인정되었다. 6. 인위적 혐기조건에 대한 각 조사형질의 품종별 평균 감소율의 차이는 l1-19%이었으며, 올쌀보리의 감소율이 가장 낮고, 올보리가 가장 높은 경향이었다. 보리의 생육반응으로 보아 품종간 반응차이가 가장 민감하게 나타나는 것으로 평가된양분부족 +$\textrm{N}_2$ 척리 +CA 3mM 조건에서의 각 품종별 유묘형질의 평균 감소율은 올쌀보리＞내한발보리=흰찰발보리＞찰발보리＞두원찰발보리＞올보리 순으로 낮았다.

• 한국환경보건학회지
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• 제16권1호
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• pp.45-53
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• 1990

To investigate the effect of cultural condition on the aflatoxin production of Aspergillus flayus ATCC 15517, mixed culture with Aspergillus niger, better kind of media and size of Cultural vessels were examined. YES medium was better than SLS medium for this study. Small scale test tube culture was showed the possibility to simply examine the growth, total acidity, pH and aflatoxin production during cultivation, and also could reduce the second contamination of aflatoxin B1 from large scale broth cultured. Especially ELISA method is simple, sensitive and specific and therefore well suited to small scale of test tube culture. Mixed culture significantly reduced the aflatoxin production of Aspergillus fiavus ATCC 15517 and showed almost 95% inhibition of that level during the incubtation.

• Toxicological Research
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• 제14권3호
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• pp.301-306
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• 1998

This experiment was carrid out to know the effects of heating and serum on hydroxyl radicals in embryonic mouse forebrain (cerebrum) culture. The heating to mouse embryonic cerebrum cells in culture was done in a water bath at 43${\circ}C$ for 60min. After that, two supernatants were prepared at 20 hrs and 48 hrs respectively after heat treatment to the brain cells. To find out the heating effects on neuron cells, mouse cerebrum cells (13 embryonic day) were cultured in hydroxyl radical generation system composed of 20mU/ml glucose oxidase (GO system), using condition of normal culture media (MEM, 5% serum, 5% $CO_2$or supernatant prepared after heating at 43${\circ}C$ for 60 min in a water bath. Supernatant prepared at 20 hrs after heat treatment had a greater protective effects against hydroxyl radical than supernatant prepared at 48 hrs after heat treatment . Otherwise, the protective effect of serum against hydroxyl radicals in the cultured brain cells is higher than that in the heat treatment. These results indicated that serum in culture media reduced cytotoxicity of hydroxyl radicals in mouse forebrain culture, also that heat treatment showed the protective effects against hydroxyl radicals generated with 20mU/ml GO system in mouse forebrain culture.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권3호
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• pp.254-261
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• 2005

The over-expression of Bcl-2 has greatly improved the culture period, specific growth rate, and maximum viable cell density of NS0 cells culture under low serum condition. Further analysis of these data suggests that a saturation model of the Monod type can be used to represent the relationships of specific growth rate and initial serum concentration. The ${\mu}_{max}$ and $K_s$ for the Bcl-2 cell line is $0.927day^{-1}\;and\;0.947\%(v/v)$ respectively, which are $21\%$ greate and $7\%$ lower respectively than its control counterpart. Study on the amino acid supplementation revealed that Bcl-2 cell lines possess greater improvement in the specific growth rate and maximum viable cell density compared to the control cell lines. A further increase in the amino acid supplementation has resulted a $17\%$ decrease in specific growth rate and no improvement in maximum viable cell density in the control culture. However, the Bcl-2 cell line exhibited a better growth characteristic in this culture condition compared to that of control cell lines. The higher specific growth rate and maximum viable cell density of the Bcl-2 cell line in medium fortified with serum and MEM EM suggested a more efficient nutrient metabolism compared to that in the control cell line. The low serum and amino acid utilisation rate and the higher cell yield may prove to be important in the development of serum/protein free culture.

• 화훼연구
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• 제26권4호
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• pp.166-178
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• 2018

In this study, the principal objective was to investigate the effect of light quality and vessel ventilation on the growth and development, physiology, activities of antioxidant enzymes, and contents of mineral nutrients of carnation (Dianthus caryophyllus L.) 'Marble Beauty'. Single node cuttings stuck into the plant growth regulator (PGR)-free MS medium in containers covered with caps with or without a ventilation filter were cultured first four weeks under white and then additional four weeks under either white (control), blue, red, or red + blue light emitting diodes (LEDs) for 56 days. Interestingly, a ventilated culture condition not only reduced the percentage of the hyperhydricity, but also increased the total chlorophyll content (Chl a + Chl b) of the plantlets as compared to the non-ventilated condition. In addition, blue LEDs produced plantlets with the greatest number of shoots and red LEDs produced plantlets with the greatest shoot length. The quality of plantlets was improved under a ventilation condition. Besides, under a ventilated condition, red + blue LEDs raised APX activity, and blue LEDs not only raised the activity of the CAT, but also increased tissue contents of such elements as K, Ca, Mg, Zn, Mn and Fe. The red LEDs increased contents of B and Si under a ventilated condition, and Na accumulation under a non-ventilated condition. Thus, including blue or red LEDs as the light source in a ventilated culture condition will produce plantlets of carnation 'Marble Beauty' in vitro with improved quality.