• Title/Summary/Keyword: reconstitution

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Two-Year Security Related Studies and Curriculum Reconstruction (2년제 경호관련학과의 교육과정 현황 및 재구성)

  • Jang, Ye-Jin
    • Korean Security Journal
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    • no.25
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    • pp.165-184
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    • 2010
  • However, the academic identity of security related studies still remain as a task to be solved. In 2000, security related studies were newly established and offered in 60 universities, but the present condition is that the number of universities that offer the studies has decreased due to the issues such as reduction of freshmen and there are currently 39 universities (both two-year and four-year). In addition, an appropriately mixed use of name of the studies is being demanded in order to respond to the social overall market changes, as the names such as 'police', 'security', 'martial art' and 'non-commissioned officer'. Of course, the common goal the studies are pursuing is safety service activities for civilians. However, the present condition is that the establishment of university system is still insufficient, and the curriculum has composed loosely by professors in various field. Therefore, the two-year security related studies with strong market directivity in the security industry were selected in this thesis to highlight the realistic issues and comparatively analyze the curriculum of each school to deduce basic curriculum and future direction was presented. The wavering curriculum of each school while seeking the common goal of security related studies is reflecting the reality of unsettled security studies and it is a task that needs to be solved. The security related studies need reinforced education such as the method to provide opportunities of joint-task among professional manpower, academic-industrial cooperation process and academic-industrial joint-development, while adopting subdivided education.

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Effect of a Phospholamban Peptide on the Skeletal Sarcoplasmic Reticulum $Ca^{2+}$ Transport (골격근 근장그물 칼슘이동에 대한 Phospholamban 펩타이드의 조절)

  • Kim, Hae-Won;Lee, Hee-Ran
    • The Korean Journal of Pharmacology
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    • v.30 no.1
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    • pp.117-124
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    • 1994
  • Phospholamban is the regulator of $Ca^{2+}-ATPase$ in cardiac sarcoplasmic reticulum(SR). The mechanism of regulation appears to involve inhibition by dephosphorylated phospholamban. Phosphorylation of phospholamban relieves this inhibition. Recently, there has been a report that the cytoplasmic domain (amino acids 1-25) of phospholamban is insufficient to inhibit the $Ca^{2+}$ pump. To explore the domains of phospholamban responsible for $Ca^{2+}-ATPase$ inhibitory activity, we examined the effect of a synthetic phospholamban peptide consisting of amino acid residues 1-25 on $Ca^{2+}$ uptake by reconstituted skeletal SR $Ca^{2+}-ATPase$. The $Ca^{2+}-ATPase$ of skeletal SR was purified and reconstituted in proteoliposomes containing phosphatidylcholine (PC) or phosphatidylcholine: phosphatidylserine (PC:PS). Inclusion of a phospholamban peptide in PC proteoliposomes was associated with significant inhibition of the initial rates of $Ca^{2+}$ uptake at pCa 6.0, and phosphorylation of this peptide by the catalytic subunit of cAMP-dependent protein kinase reversed the inhibitory effect on the $Ca^{2+}$ pump. Similar effects of phospholamban peptide were also observed using PC:PS proteoliposomes. Based on these results, we could conclude that the cytoplasmic domain of phospholamban, containing the phosphorylation sites, by itself is sufficient to inhibit the $Ca^{2+}$ pump of SR.

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Freeze Drying of Lactic Acid Bacteria Fermented Food Prepared from Egg White Powder and Casein Supplemented with Growth Stimulating Agent (생육촉진물질이 첨가된 난백분말과 카제인으로 만든 젖산균 발효식품의 동결건조)

  • Ko, Young-Tae;Lee, Eun-Ju
    • Korean Journal of Food Science and Technology
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    • v.31 no.5
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    • pp.1337-1344
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    • 1999
  • Lactic acid bacteria fermented foods were prepared from egg white powder, casein and growth stimulating agent. pH change and growth of Lactbacillus acidophilus(KCTC 2182) during freeze drying were studied. The effects of freeze drying on sensory evaluation. hardness and volatile aroma compounds in freeze dried sample or reconstituted sample were also studied. Freezing and freeze drying did not affect pH of fermented samples. Number of viable cells in original fermented samples was markedly reduced during freezing or freeze drying. When number of viable cells in original fermented sampler was considered at 100%. survival ratio of viable cells after freezing was $72.0{\sim}82.4%$ and that after freeze dying $10.0{\sim}20.4%$. When sensory properties of original fermented samples were compared with those of freeze dried/reconstituted samples, sensory properties of original samples were generally better than those of freeze dried/reconstitute samples. However, the reconstitution property and the acceptability of freeze dried samples were good. Volatile aroma compounds in original fermented samples were reduced during freeze drying. The reduction degree of volatile aroma compounds varied with sample.

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The Development and Application of Practical Problem-based Lesson Plan on Consumer Choice of Genetically Modified Food - Focused on the 'Dietary Life' in High School Home Economics - (유전자변형식품 선택의 실천적문제중심 교수.학습 과정안 개발 및 적용 - 고등학교 식생활 영역 -)

  • Kang, Kyung-Hwa;Kim, Young-Nam
    • Journal of Korean Home Economics Education Association
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    • v.22 no.2
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    • pp.101-113
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    • 2010
  • The purpose of this study is to develop and evaluate the practical problem-based lesson plan for high school home economics class focusing on consumer choice of genetically modified(GM) food. The ADDIE instructional design model was applied. The first analysis step, practical problems were chosen based on the previous studies and the educational goal suggested in the 2007 revised curriculum. The next design and development steps, 4 hours of lesson plans were developed by reconstitution a chapter of Food Safety. Additional learning materials(17 student activity papers, 17 student reading materials, 3 teacher reading materials) were also developed. The implementation step, the lesson plans developed were applied to the S high school students in Bundang, Geyonggi-do. The last evaluation step, the perception change about GM food and the adequacy of teaching method were evaluated. As results, the studens' attitudes and minds towards the GM food were changed. The level of concern on GM food increased, and the ratio of students who would check whether GM food or not was increased. Also students showed high level of satisfaction on teaching method and materials.

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Survey of Yogurt Powder Storage in Ambient Export Countries A Safety Evaluation Standard Compliance and Comparative Analysis

  • Kim, Na-Kyeong;Park, Jung-Min;Lee, Jung-Hoon;Kim, Ha-Jung;Oh, Sejong;Imm, Jee-Young;Lim, Kwang-Sei;Kim, Jin-Man
    • Food Science of Animal Resources
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    • v.35 no.1
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    • pp.143-148
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    • 2015
  • Yogurt powder is fermented milk processed in the form of dry yogurt, and has advantages such as stability, storability, convenience, and portability. China and Vietnam are important export target countries because of the increased demand for dairy products. Therefore, we surveyed dairy product standardization in order to establish an export strategy. Lactic acid bacteria counts are unregulated in Korea and Vietnam. In China, lactic acid bacteria counts are regulated at $1{\times}10^6$ colonyforming units (CFU)/mL and detected at $6.24{\pm}0.33\;Log\;CFU/mL$. All three countries have regulated standards for total bacterial counts. In China, total bacterial counts of milk powder are regulated to n=5, c=2, m=50,000, M=200,000 and detected at $6.02{\pm}0.12\;Log\;CFU/mL$, exceeding the acceptable level. Lactic acid bacterial counts appeared to exceed total bacterial counts. Coliform group counts, Staphylococcus aureus, Listeria monocytogenes, and Salmonella species were not detected. Acidity is not regulated in Korea and Vietnam. In China, acidity was regulated to over $70^{\circ}T$ and detected $352.38{\pm}10.24^{\circ}T$. pH is unregulated in all three countries. pH was compared to that of general fermented milk, which is 4.2, and that of the sample was $4.28{\pm}0.01$. Aflatoxin levels are not regulated in Korea and China. In Vietnam, aflatoxin level is regulated at 0.05 ppb. Therefore, all ingredients of the yogurt powder met the safety standards. This data obtained in this study can be used as the basic data in assessing the export quality of yogurt powder.

A Study on Transformational stream in Space and Time of the Animation - Focusing on the Georges Schwizgebel's Works - (애니메이션 시공간의 변형적 흐름에 관한 연구 - 조르주 슈비츠게벨의 작품을 중심으로-)

  • Kim, Hong-Kyun
    • Cartoon and Animation Studies
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    • s.45
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    • pp.75-99
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    • 2016
  • Unlike the other media, the animation has its unique aesthetic characteristics. First, one of the media characteristics of the animation is creativity images of the hand drawn animation by shooting of frame by frame. Next the animation image which is created through metamorphosis obtain new space-time by unique motility. This paper treats of two parts of space and time in animation. One is the metamorphosis image of animation and the other is the motility. To concrete this, this paper analyzed Georges Schwizgebel's Works. They are very interesting that the structural characteristics of the animation. Especially, , < La course ${\grave{a}}$ $l^{\prime}ab{\hat{i}}me$ > directed by Georges Schwizgebel are a good text for analyzing space-time of animation in that it combines various metamorphosis and motility with his expressions. The images of his works are continuously maintained transformational stream by his main expressions of metamorphosis and motility etc. This study focuses on analyzing the way of metamorphosis and motility function to make meaning in this texts. His works consist with metamorphosis and motility, and various moving images to let audience feel the characteristics of space and time in animation. We can experience numerous dual structures like space and time, freeze-frame and motility, rhythm and repetition, two dimension and three dimension, dissolution and reconstitution, abstract shape and concrete form etc from these cases. One of the main apparat is the emphasis of metamorphosis and motility by independent space of animation.

Clinical Significance of Immature Reticulocyte as an Early Recovery Indicator after Bone Marrow Transplantation (골수이식 후 미성숙 망상적혈구의 유용성 평가)

  • Seo, Suk Won;Kim, Chun Hee;Chi, Hyun Sook
    • Korean Journal of Clinical Laboratory Science
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    • v.36 no.1
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    • pp.27-32
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    • 2004
  • Bone marrow transplantation(BMT) is widely used as curative means of various malignant and nonmalignant hematologic disorders, and early and accurate determination of engraftment is very important for critical management decisions. Reticulocyte counts performed by automated flow cytometric methods is a good indicator of erythropoietic activity and its evaluation has been proposed as an early predictor of bone marrow regeneration. Some reports highlighted the usefulness of the percentage of highly fluorescent reticulocytes and the sum of highly and medium fluorescent reticulocytes(immature reticulocyte fraction, IRF). In Asan Medical Center, the criteria for engraftment following BMT or PBSCT was defined as the first day of a 3-day trend of absolute neutrophil count(ANC)${\geq}500/uL$ and platelet count${\geq}30{\times}10^3/uL$. In 1999, Grotto et al proposed an indidator of bone marrow recovery as the first day on which the IRF was twice the minimum value after bone marrow transplantation. To compare the both criterias, we got consecutive datas of immature reticulocyte fraction, absolute neutrophil count(ANC), WBC count, platelet count and reticulocyte count by XE-2100 automated hematology analyzer(Sysmex Co. Japan) from 33 patients daily after BMT. When compared to standard neutrophil engraftment(10-30 days, $16.2{\pm}4.6days$), IRF engraftment (5-21 days, $11.0{\pm}3.9days$) occured significantly earlier in 87.9% of patients(P<0.05). The mean engraftment day for WBC count(11-29 days, $16.4{\pm}4.3days$) was similar to ANC, but platelet count and reticulocyte count revealed more delayed data (10-49 days, $19.1{\pm}7.4days$ vs 17-64 days, $31.4{\pm}14.1days$). In conclusion, our results confirm that an increase in the immature reticulocyte population is the earliest sign of the hematopoietic recovery after BMT and that automated reticulocyte quantification including immature fraction may be integrated into clinical protocols to evaluate bone marrow reconstitution.

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Change of Stratification of Three Dimensional Culture by Gingival Keratinocytes & Fibroblasts (치은 각화상피세포와 섬유아세포를 이용한 삼차원적 배양시 중층화 동안의 변화)

  • Jung, Tae-Heup;Hyun, Ha-Na;Kim, Yun-Sang;Kim, Eun-Cheol;You, Hyung-Keun;Shin, Hyung-Shik
    • Journal of Periodontal and Implant Science
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    • v.32 no.1
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    • pp.129-142
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    • 2002
  • Epithelial-mesenchymal interaction plays a important role in cell growth and differentiation. This interaction is already well known to have an importance during the organ development as well as cell growth and differentiation. However, in vitro experimental model is not well developed to reproduce in vivo cellular microenvironment which provide a epithelial-mesenchymal interaction. Because conventional monolayer culture lacks epithelial-mensenchymal interaction, cultivated cells have an morphologic, biochemical, and functional characteristics differ from in vivo tissue. Moreover, it's condition is not able to induce cellular differention due to submerged culture condition. Therefore, the aims of this study were to develop and evaualte the in vitro experimental model that maintains epithelial-mesenchymal interaction by organotypic raft culture, and to characterize biologic properties of three-dimensionally reconstituted oral keratinocytes by histological and immunohistochemical analysis. The results were as follow; 1. Gingival keratinocytes reconstituted by three-dimensional organotypic culture revealed similar morphologic characteristics to biopsied patient specimen showing stratification, hyperkeratinosis, matutation of epithelial architecture. 2. Connective tissue structure was matured, and there is no difference during stratification period of epithelial 3-dimensional culture. 3. The longer of air-exposure culture on three-dimensionally reconstituted cells, the more epithelial maturation, increased epithelial thickness and surface keratinization 4. In reconstitued mucosa, the whole epidermis was positively stained by anti-involucrin antibody, and there is no difference according to air-exposured culture period. 5. The Hsp was expressed in the epithelial layer of three-dimensionally cultured cells, especially basal layer of epidermis. The change of Hsp expression was not significant by culture stratification. 6. Connexin 43, marker of cell-cell communication was revealed mild immunodeposition in reconstitued epithelium, and there is no significant expression change during stratification. These results suggest that three-dimensional oragnotypic co-culture of normal gingival keratinocytes with dermal equivalent consisting type I collagen and gingival fibroblasts results in similar morphologic and immunohistochemical characteristics to in vivo patient specimens. And this culture system seems to provide adequate micro-environment for in vitro tissue reconstitution. Therefore, further study will be focused to study of in vitro gingivitis model, development of novel perioodntal disease therapeutics and epithelial-mensenchymal interaction.

A Study on the Signal Control Unit's Reconstitution to Control the Separated Through/Left(or Right) Turn and the Median Bus Lane Signal for the Standard Traffic Signal Controller (표준규격 교통신호제어기에서 교통류별 전용신호 구현방안 연구)

  • Han, Won-Sub;Lee, Ho-Won;Hyun, Cheol-Seung;Joo, Doo-Hwan;Lee, Choul-Ki
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.8 no.5
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    • pp.57-70
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    • 2009
  • According to the change of the traffic conditions, the requirement of controlling the separated left(or right) turn, the median bus lane and bicycle signal is increasing. However, the traffic signal controller standard based on the four-set lights restricts to control the three-set lights which control the separated direction and kind of traffics. This study suggests the method to control the three-set lights signal by improving the hardware and software for the traffic signal controller which is operated currently in the traffic scene. The 6 output ports of Load Signal Unit(LSU) which is consisted of Pedestrian Red, Pedestrian Green, Red, Yellow, Arrow and Green signal reconstitute 2 rows of the Red, Yellow, Green for three-set lights while the Signal Map data's code values which control the signal step of an individual Ring and LSU are established to adjust the LSU's output ports(R1 Y1 G1 R2 Y2 G2) of the three-set lights. The effect of using the separated through/left turn and the median lane bus signal of three-set lights is analyzed from a Mangwoo intersection in Seoul. The results of analysis show that the delay time of the east-west direction where the median bus lane is operated is especially improved with over 70 sec/veh.

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A Study on Retro-look Fashion Appeared in 1990′s -With Special Reference to The Revival of 1960′s Mode- (1990년대에 패션의 복고풍에 관한 고찰 -1960년대 Mode의 재현을 중심으로-)

  • 류숙희;박종희
    • The Research Journal of the Costume Culture
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    • v.4 no.2
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    • pp.247-263
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    • 1996
  • This study focuses on a comparative study of 1960s'retro look mode in terms of the past and present in order to find out in detail how it in the past is readjusted after it was appeared in the present. For such a study, in the first place the contents of the dress and its ornament of a retro-look fashion was refined through some literature, and then, some works of the dress and its ornament of a retro-look fashion was refinded through some literature, and then, some works of eh dress and its ornament revived in 1960's mode were analysed, based on some fashion magazines at home and abroad like Bazaar, Fashion etc News in 1990s. After 1960s'retro-look mode which reappeared in 1990s was researched in terms of silhouette, detail, texture, color, and pattern, differences between those tow periods of 1960s and 1990s and their causes are summarized as follows: 1. In the aspect of silhouette, it appears that the silhouette in 1960s is that of somewhat stiff, charming image in which Body is excluded and the silhouette in 1990s is that of an soft, feminie image in which Body is emphasized. It was understood that the cause of such a delicate difference comes from the influences of the change in aesthetic senses or awareness, naturalism and neo-feminism. 2. In the aspect of detail, it appears that the detail in 1990s is of an attempt to express in diverse images, compared to that in 1960s, and new images are created new image in 1990s by means of presenting entirely ill-matched images. The major cause of that is because of Antistandard fashion. 3. In the aspect of textures, it appears that a great feature is that the texture in 1990s is of that introduced, being changed in natural and high-class looks, compared to that of 1960s. It was reviewed that the major cause of this is because of a result from the influence of naturalism and the technical growth in various fields which has brought the development of dress material. 4. In the aspect of color, it appears that the color in 1990s is of an image of primary color which is far more sensual and feminie than that of 1960s. It was studied that the major cause of ti comes from the influence of neo-feminism, etc. 5. In the aspect of pattern, it appears that the pattern in 1990s is of that of symbolism, transposition, and the ecletic feature of various modes which appear more deeply than that of 1960s. It was studied that the major cause of such changes is because of a trend of postmodernism which has brought the change of the spiritual structure different from that in the age of modernism. In conclusion, it was understood that the retro-look fashion is of an expression technic of dress and its ornament in that o dress in the pst is simply imitate, but new reconstitution is done by using the elements in the past. at the same time, ti was clarified that even though the elements in the past are revived as they were, dress and its ornament is governed by the social and cultural environments of he day, and with this proof it can be said that the fashion in each age is of a reflection of social phenomena of that age.

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