• 대한수의학회지
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• 제43권2호
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• pp.203-210
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• 2003

The purpose of this study is to investigate the pharmacokinetics and tissue distribution of recombinant bovine somatotropin (rBST) after subcutaneous adminstration of $^{125}I-rBST$ in male and female rats. A solid state conjugation (Iodo-bead$^{(R)}$) method was confirmed useful for producing $^{125}I-rBST$ because the administration of the conjugated form enabled enough to determine time- concentration relationships of rBST in rats. Subcuatenous administrations showed sex differences that female ($t_{1/2,kc}$, 2.87 h) revealed rapid elimination as compared to male ($t_{1/2,ke}$, 4.81 h), with the absorption ($t_{1/2,ka}$ in male being 0.3 h and that in female 0.75 h) in the reverse order. For subcutaneous administration of rBST in male rats, the liver was the highest in amount, followed by kidney, testes, muscle, and stomach, at the slaughtering tame of 1, 6, 12 and 24 h. But the testes was the highest at the 48 h slaughtered animals, followed by liver, kidney, stomach, and muscle. In slaughtered females at 1, 6, and 12 h after the administration of rBST, the liver was the highest, followed by ovary, kidney, small intestine, and stomach. At 24 and 48 h slaughtered female rats, the ovary was the highest, the liver the second, and the kidney the third.

• 한국양식학회지
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• 제12권2호
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• pp.79-89
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• 1999

The recombinant bovine somatotropin (rBST) was administered to the oliver flounder, Paralichthys olivaceus, to know the effects and optimal administration frequency and dosage of the hormone. The experiment was conducted with three different treatment groups (A, B and C) designated based on the duration and administration frequency of rBST and one control (D) from April 14, 1996 to March 16, 1997. The fish of hormone treated groups grew 7.86 to 10.07% (47.45 to 60.75 g in weight) better than the control at the end of the experiment (P<0.05). No significant differences in their growth were detected among treatment groups. The distinct growth improvement was recognized four weeks after completion of the first four hormone administration. When considering water temperatures measured from the experimental tanks, the effect of rBST on the flounder was greater during the period showing relatively lower temperature. The survival rates were higher in treatment groups than in the control, revealing 98.3% (A), 98.4% (B), 97.7% (C) and 93.1% (D) during the first stage of culture; 92.7% (A), 91.3% (B), 86.7% (C) and 80.0% (D) during the second stage of culture.

• 한국임상수의학회지
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• 제39권1호
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• pp.38-43
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• 2022

A 5-month-old Holstein downer calf was presented, and bovine viral diarrhea virus was detected in the feces and the cerebrospinal fluid. Combined treatment of recombinant bovine somatotropin (rBST) administration and standing rehabilitation had been performed for two months, and the calf could maintain the standing position for a while. However, the symptoms were not improved, and the calf died due to ruminal tympany and aspiration pneumonia. Megaesophagus, abomasal ulcer, and a normal-sized but softened cerebellum were observed in clinical necropsy. Improvement of symptoms was confirmed by rBST, but was not reached the complete recovery of gait and standing position.

• 한국가축번식학회지
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• 제20권2호
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• pp.197-205
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• 1996

This study was conducted to examine the effect of sustained release recombinant bovine somatotropin(SR-rBST) with different dosage(0, 0.03mg/kg body weight/14 day, 0.06mg/kg body weight/14 day, 0.09mg/kg body weight/14 day) on the economical traits of the semen characteristics and sexual behaviors and blood chemical values with sixteen Holstein dairy young bulls in Dairy Cattle Improvement Center of National Livestock Co-operatives Federation. Sensual testings of collected semens of Holstein young bulls were not different among treatments (p>0.05). Ejaculated semen volumes of control, SR-rBST 0.03mg, 0.06mg and 0.09mg groups were 5.6ml, 5.6ml, 6.0ml and 6.7m, respectively, but the result of SR-rBST 0.09mg group significantly increased semen volume(P<0.05). In sperm concentration, SR-rBST 0.06mg and 0.09mg groups significantly increased the total number of sperms than the other groups(P<0.05). In otherwise, SR-rBST treatments did not affect on pH, osmotic pressure, anti forzen rate, abnormality and motility of collected semen during whole experimental period. Reaction time(RT), sexual aggressiveness(SA) and tactile stimulation(TS) were not different among treatments. The libido scores(LS) of control, 0.03mg, 0.06mg and 0.09mg groups were 68.0, 80.5, 73.9 and 80.8, respectively, and LS were significantly improved by SR-rBST administration(P<0.05). The effect of SR-rBST on scrotal circumference measurement that is important factor to determine the ability of semen production, was not different among treatments. These studies indicates that SR-rBST treatments favourably affect on semen quality and sexual libido in Holstein young bull.

• Fisheries and Aquatic Sciences
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• 제11권4호
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• pp.183-189
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• 2008

In this study, tests were conducted to investigate the effects of three dietary growth hormones, administered in various amounts, on the growth performance and lysozyme activity in juvenile olive flounder, Paralichthys olivaceus. Three dietary growth hormones, recombinant human growth hormone (rHGH), recombinant bovine somatotropin A (rBST A) and recombinant bovine somatotropin B (rBST B) were tested at three different supplemental levels (10, 20 or 40 mg/kg body weight per week) by a $3{\times}3$ factorial design and a complete randomized design in comparison to a control group. Fish were fed one of the ten experimental diets (control, $rHGH_{10}$, $rHGH_{20}$, $rHGH_{40}$, rBST $A_{10}$, rBST $A_{20}$, rBST $A_{40}$, rBST $B_{10}$, rBST $B_{20}$ and rBST $B_{40}$) for 6 weeks and afterward were analyzed for growth performance by measuring weight gain (WG), feed efficiency (FE), specific growth rate (SGR) and protein efficiency ratio (PER). Based on the factorial design analysis, fish fed rHGH diets demonstrated significantly higher growth performance than fish fed rBST A or rBST B diets. However there were no significant differences in WG, FE, SGR and PER between fish fed rBST A and rBST B diets. Neither hormone level nor the interaction between the different hormones and their various levels had a significant effect on WG, FE, SGR, PER, lysozyme activity or whole-body proximate composition. A complete randomized design analysis confirmed fish fed $rHGH_{10}$, $rHGH_{20}$, $rHGH_{40}$, rBST $A_{10}$, rBST $A_{20}$, rBST $A_{40}$, rBST $B_{20}$ and rBST $B_{40}$ diets for 6 weeks showed higher WG than fish fed the control diet (P<0.05). A higher FE was observed in fish fed $rHGH_{10}$, $rHGH_{20}$, $rHGH_{40}$, rBST $A_{20}$ and rBST $A_{40}$ diets in comparison to fish fed the control diet. Fish fed all graded rHGH, rBST A and rBST B supplemented diets showed a higher SGR than fish fed the control diet. Regarding PER, fish fed $rHGH_{10}$, $rHGH_{20}$, $rHGH_{40}$, rBST $A_{10}$, rBST $A_{20}$, rBST $A_{40}$ and rBST $B_{20}$ diets were higher than fish fed the control diet. Furthermore, the lysozyme activity of fish fed a diet of $rHGH_{20}$ was significantly higher than that of fish fed any other diet. The results measuring the growth and development of the fish clearly suggest the biopotency of dietary rHGH could be higher than those of both dietary rBST A and rBST B. Further implied is the probability that within the range of 10 to 40 mg/kg BW/week the dietary growth hormones could accelerate growth performance, and that 20 mg rHGH/kg BW/week could possibly enhance lysozyme activity in juvenile olive flounder, Paralichthys olivaceus.

• Journal of Animal Science and Technology
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• 제47권3호
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• pp.439-446
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• 2005

This study was conducted to elucidate the influence of recombinant bovine somatotropin (rBST) on the plasma IGF-I profiles in deer stags. Slow release rBST (250mg each) preparations were injected subcutaneously during the antler's non-growing period and growing period. It was observed that significant increases in the plasma IGF-I concentrations were occurred right after injections of the rBST irrespective of antler's non-growing period or growing period. When the rBST preparations were injected two times with one week interval during the antler's non-growing period, the mean level of IGF-I was found to be 2,920.7ng I mL, indicating that the IGF-I concentration increased up to 8.4-folds compared with a control. After two weeks from second shot, however, the IGF-I levels decreased to the level of the control. Similar results were also obtained with both a Sika and a Red deer during the antler's growing period. Increases in the IGF-I level of up to 5.6-folds with the Sika deer and 4.3-folds with the Red deer were found. Based on the present study, it is likely that the rBST may be used as a possible candidate for an antler's growth stimulating agent.

• Journal of Pharmaceutical Investigation
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• 제32권3호
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• pp.199-207
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• 2002

In order to develop a sustained release formulation of bovine somatotropin (BST), which has been used to increase the body weight of oxen or the milk production of dairy cows, poly(D,L-lactide-co-glyceride)(PLGA) microspheres were made by W/O/W multiple emulsification method and solvent extraction method. Physical properties including particle size, drug entrapment, drug release, protein denaturation, and in vivo body weight increase in rats were characterized. The size of the microspheres was increased as the molecular weight of PLGA increased. When Span 65 and stearic acid during preparation were added, the size was decreased but the amount of surface protein was increased, resulting in a high loading efficiency, with fast release of BST from the microspheres. Aggregation or fragmentation of BST by SDS-PAGE during microsphere preparation and drug release study was not observed. Body weight of Sprague-Dawley's male rats was significantly increased after subcutaneous administrations of BST-loaded PLGA microspheres. There was a good correlation between in vivo weight gain and in vitro release rate of microspheres. PLGA microspheres with a high surface protein ratio could be a good candidate for the sustained delivery of BST.

• 한국임상수의학회지
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• 제16권2호
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• pp.332-338
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• 1999

This study was carried out to investigate weight gain and prevention of diarrhea in suckling calves which were injected with recombinant bovine somatopropin (rBST). A total of 101 breeding cows were assigned to the six groups according to the administered dosage and injected time, respectively. Groups T-1, T-3, T-4 and T-6 were injected starting 1 week before calving and groups T-2 and T-5 were injected on the calving day. The six groups were injected five times at two week intervals. Groups T-1 and T-2 were injected with 250mg composite rBST, Group T-3 was injected with 375 mg composite rBST. Groups T-4 and T-5 were injected with 500mg composite rBST, And group T-6 was injected with 500mg rBST-S. The control group was not injected with BST. The groups injected with 500 mg BrST had a lower rate of morbidity from diarrhea than the control group or the groups injected with 250 mg rBST (T-1 and T-2). Weight gain was higher in group T-4 than in the control group or groups T-1 and T-2. In Korean Native Cattle, the total weight gain was greater in group T-4 than in the control group (p<0.05). In crossbred cows, total weight gain was the highest in group T-4, and the total weight gain rate was greater than in group T-4 and the control group (p<0.05). The results of the hematological values showed that injections of rBST did not affect the level of the RBC, TP and BUN in the breeding cows at 9 weeks after postpartum or the neonatal calves. The results of this study indicate that injecting breeding cows with 500 mg rBST before calving would be effective in the preventing of diarrhea and in increasing weight gain of calves from birth to weaning.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2004년도 춘계학술발표대회
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• pp.290-290
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• 2004

유전자재조합 소 소마토트로핀(recombinant bovine somatotropin, rBST)은 인슐린양성장인자(IGF)-1의 합성과 분비량 조절에 의해 당과 아미노산의 흡수 및 단백질합성의 촉진을 통한 체내흡수 영양소 이용효율 증가, 산유량 증진 및 증체 효과, 세포증식의 촉진작용 등이 있는 것으로 보고(Bauman, 1982; Mears, 1995; 권 등, 1997)된 바 있으며, 또한 최근에 젖소 및 육우빈우의 인공수정시 rBST를 투여하였을 때 1회 수정수태율, 혈중 progesterone 농도 및 IGF-1 농도 등에 영향을 미치는 것으로 보고하였다(Lucy 등, 1994; Bilby 등, 1999; Morales-Roura 등, 2001). (중략)

• Asian-Australasian Journal of Animal Sciences
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• 제10권2호
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• pp.229-232
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• 1997

Effects of short-term treatment with somidobove (recombinantly produced bovine somatotropin, BST) on estrous cyclicity and fertility were studied in dairy buffaloes. Twenty buffaloes of Nili-Ravi breed calving during the same season were assigned to either control (n=8) or treated group (n=12). The buffaloes of treated group received single infection (prolonged release) of 320 mg of somidobove on day-60 postpartum. The mean values for interval to first postpartum estrus, first service conception rate, services per conception, service period and calving interval for the treated group were 96.4 days, 66.7%, 1.70, 164 days and 473 days, respectively. The corresponding values for the control group were 92.5 days, 62.5%, 1.87, 135 days and 439 days. Means of all variables did not differ between control and treated group (p > 0.05). Three buffaloes of the control and four buffaloes of the treated group did not conceive at first service. Out of these, two buffaloes of control and one buffalo of treated group exhibited normal estrous cycles. It is concluded from these data that short term BST-treatment has no adverse effect on reproductive functions of dairy buffaloes.