• Research in Plant Disease
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• v.15 no.2
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• pp.83-87
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• 2009

Direct Polymerase Chain Reaction (PCR) method that combines biological and enzymatic amplification of PCR targets was developed for the detection of Xanthomonas axonopodis pv. glycines on soybeen seeds without DNA isolation. Primers Xag F1 and Xag R1 were designed to specifically amplify a 401 bp fragment of the glycinecin A gene of X axonopodis pv. glycines. Xag F1 and Xag R1 were used to carry out the PCR analysis with genomic DNA from 45 different bacterial strains including phylogenetically related bacteria with X axonopodis pv. glycines, and other bacterial strains of different genus and species. The PCR assay using this set of primers were able to detect X axonopodis pv. glycines with DNA concentration as low as 200 fg and $1.8{\times}10^3$ cfu/ml. The Xag was detected from the seed samples incubated for 2 hrs with shaking and the intensity of the band was increase with the incubation time of seeds. The Direct PCR assay method without DNA isolation makes detection of X. axonopodis pv. glycines on soybean seeds easier and more sensitive than other conventional methods. The developed seed assay using direct PCR method will be useful for the specific detection of X. axonopodis pv. glycines in soybean seed samples.

• Korean Journal of Soil Science and Fertilizer
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• v.42 no.6
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• pp.415-422
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• 2009

Effect of soil salinity variation on the growth of barley, rye and oat was studied at the Hwaong, Iweon and Yeongsangang reclaimed lands in the western seaside of Korea. Soil salinity variation and soil EC were very high for crops to be killed or to be brought serious growth retardation during the growing season at the Iweon and Hwaong reclaimed lands, but fully low not to bring growth retardation at the Yeongsangang reclaimed land. Relation between soil salinity and crop growth and yield was well expressed as logarithmic function. Surface soil EC to reach at 50% of seed-emergence reduction was estimated $6.5dS\;m^{-1}$ for barley and $5.1dS\;m^{-1}$ for rye and oat by logarithmic function. In addition, surface soil EC to reach at 50% of grain yield reduction to the best growth in the experimental site was estimated $5.6dS\;m^{-1}$ for barley, $5.8dS\;m^{-1}$ for rye and $5.7dS\;m^{-1}$ for oat, while soil EC to reach at 50% of dry matter reduction was estimated $5.5dS\;m^{-1}$ for barley, $6.2dS\;m^{-1}$ for rye and $5.8dS\;m^{-1}$ for oat by logarithmic function. Grain yield of barley, rye and oat was 395, 164 and $325kg\;10a^{-1}$ in the Yeongsangang reclaimed lands naturally controlled below condition of $6dS\;m^{-1}$ of soil EC, but no harvest was obtained in the Hwaong and Iweon reclaimed land because of high salinity more than $15dS\;m^{-1}$ in maximum soil EC during growing period. Consequentially, it was concluded that soil salinity must be controlled below $6dS\;m^{-1}$ for good growth and high yield of winter barley, rye and oat in the reclaimed land in Korea.

• Korean Journal of Soil Science and Fertilizer
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• v.42 no.6
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• pp.447-453
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• 2009

Vegetation distribution and soil salinity were surveyed on the conservation plot in the Daeho reclaimed tidal land, in where the plants species distribution was more various than a periodically inundated tidal flat and the early stage of reclamation. According to the soil salinity where the vegetation patches were occurred, the mono patches of Salicornia europaea, Suaeda maritima, and Suaeda glauca were distributed in the average range of 31.05 dS/m in soil salinity, the mixed patches of them were distributed in the average range of 42.75 dS/m. Therefore, Salicornia europaea, Suaeda maritima, and Suaeda glauca showed strong salt tolerance. The mono patches of Aster tripolium, Sonchus brachyotus, and Scirpus planiculm were distributed in the range of 11.73 dS/m in soil salinity, and the mixed patches were distributed in the average range of 9.43 dS/m. Therefore Aster tripolium, Sonchus brachyotus, and Scirpus planiculmis showed moderate salt tolerance. The mono patches of Imperata cylindrica, Trifolium pratense, Miscanthus sinensis, Setaria viridis, and Trisetum bifidum were distributed in the range of 2.42 dS/m in soil salinity. These species showed characteristics of glycophytes with weak salt tolerance. The distribution of vegetation patches was influenced by the soil salinity as pioneer halophytes patches occurred at higher soil salinity zone than facultative halophytes patches, glycophytes patches occurred at lower soil salinity zone than facultative halophytes. These results suggested that occurrence of plant species and plant distribution type might be useful index to evaluate the soil salinity and desalinization in the reclaimed land of the midwest coastal area of Korea.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.spc1
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• pp.47-51
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• 2006

This study was conducted to establish the optimum seeding rate in different soil salinity level for yield stability of broadcasting on flooded paddy surface to the reclaimed saline land of south-western part at Gyehwado substation of the Honam Agricultural Research institute in $2003{\sim}2004$. Soeganbyeo was tested in the Munpo series (fine sand loam) the results obtained is as follows: As seeding rate was higher, the number of seeding stand was increased and the number of seeding stands in the low salinity field is sharply increased than those of the medium salinity field. The length of culm in medium salinity field tends to be shorter than that of the low salinity field and as seeding rate was increased, the lodging is severe. The milled rice yield was increased as up to 9 kg/10a in low and medium salinity soil. Complete rice was no significantly increased over 5 kg/10a seeding rate in low salinity field and over 7 kg/10a seeding rate in medium salinity field. Considering the yield of milled and complete rice, seeding stand and lodging, The proper seeding rate is $5{\sim}7kg/10a$ in low salinity and $7{\sim}9kg/10a$ in medium salinity for broadcasting on flooded paddy surface at the reclaimed saline land of southwestern part.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.5
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• pp.529-539
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• 2010

In order to evaluate the effect of soil salinity and culturing conditions including compost application and vinyl mulching on the maintenance of ridge and the growth of upland crops, three crops such as corn, soybean and sweet potato were experimented by using three or four cultivars of crops in the Saemangeum reclaimed tidal land. Average soil salinity before seeding was 2 dS $m^{-1}$, which was low enough for general upland crops to grow. However, high soil EC more than 16 dS $m^{-1}$ was observed in some parts of the experimented field. In the experiment, it was concluded that growth retardation and yield reduction of summer upland crops might be from severe erosion of ridge, soil compaction, flooding or wet soil condition and high salinity of some parts, and then these deteriorations were possibly improved for good crop growth and yield increase by compost application and vinyl mulching cultivation in the Saemangeum reclaimed tidal land.

• Korean Journal of Soil Science and Fertilizer
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• v.40 no.4
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• pp.330-348
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• 2007

Soil problems and agricultural water management of the reclaimed land in Korea were reviewed through research results conducted in RDA and ADC. According to the Korean Soil Classification and Soil Survey(NIAST, 2002), the 5 soil orders with the 45 soil series were distributed on the fluvio-marine or marine deposit of the west and south coastal plains. Yeompo, Munpo, Hasa, Gwangwhal, and Poseung soil series were most commonly distributed soil on the fluvio-marine deposits, associated with tideland of the sea coast. Former 4 soils were Entisols, and the latest one was the Inceptisols. Buyong soil associated with Poseung series was an Alfisols. Extent of Myeongji soil, a Molisols, and Yongho soil, a Histosol, were minor. Salinity control and management problems were closely related with high water table and low percolation rate due to plow-pan layer developed during the leaching process in the silty textured soil. For evaluation of field salinity, use of an electromagnetic inductance, EM38, with GPS was helpful to understand salinity status and field variability. Deep plowing, subsoiling and drainage improvement by tile drainage might be effective in paddy with plow-pan. New technology such as variable rate fertilization might save fertilizers and thus reduce environmental impact of agriculture on water quality. Water quality of agricultural water resources in reclaimed land was less adequate than that of inland water resources. Proper crop management is necessary depended upon quality for crop growth as well as to match with water quality target.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.spc1
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• pp.46-50
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• 2005

This study was carried out to investigate the proper nitrogen fertilizer level at reclaimed saline land in the southwestern area of Korea from 2002 to 2004. The rice cultivars tested were Samcheonbyeo(Early maturing one), Nampyeongbyeo (Medium maturing one) and Hwaseongbyeo(Mid-late maturing one). The results aye summarized as follows: The more the nitrogen level, the more number of panicle and spikelet per unit area was. But, ripened grain rate and 1000-grain weight were decreased at higher nitrogen level. As higher nitrogen level, head rice rate was decreased and protein content was increased. But, amylose content wasn't differ among the nitrogen fertilizer application levels. As increase nitrogen level in head rice yield increased up to 12 kg/10a, 11 kg/10a, in Samcheonbyeo, Hwaseongbyeo and Nampyeongbyeo, respectively. But there wasn't different more than it. Consequently, considering the yield of head rice, ripened grain rate and rice quality. The proper nitrogen fertilizer application level was 11-12 kg/10a at reclaimed saline land in the southwestern area of Korea.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.57 no.2
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• pp.166-170
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• 2012

This research was carried out to choose the suitable seedling type to transplanting in order to stabilize rice yield. The brief of this research to transplant the seedling divided into 10-day seedling, 20-day seedling, 30-day seedling, 33-day pot seedling in the field of Saemangeum Gyehwa, newly reclaimed land were following; Average salt content in 2 years was reduced from 0.16% to 0.04% after transplanting. Heading date of seedling type was Aug. 14 at 33-day pot seedling compared to 2 days at 30-day seedling, 3 days at 20-day seedling, 5 days late at 10-day seedling. Lodging index was highest at 30-day seedling, and also, field lodging was 50%. Ripening ratio and 1,000 grain weight were similar and panicle number was higher 20-day seedling > 10-day seedling > 33-day pot seedling > 30-day seedling. Rice yield was higher 10-day seedling = 20-day seedling > 33-day pot seedling > 30-day seedling. Head rice ratio was higher in 20-day seedling > 30-day seedling = 10-day seedling > 33-day pot seedling. Amylose content and protein content was not significantly different between seedling types. With this results, the suitable seedling types in newly reclaimed land are 10-day and 20-day seedling.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.56 no.2
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• pp.177-181
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• 2011

This research was carried out to establish perfect-drainage time in order to stabilize rice yield and improve rice quality. Treatments of perfect-drainage were conducted 5 days interval during 25 days to 50 days after heading date in the field of Saemangeum Gyehwa, newly reclaimed land. Accumulated temperature after heading date in 2010 increased about $100^{\circ}C$ and precipitation amount decreased a little compared to normal year harvesting time. Average panicle number was 16.5 and spikelet per panicle was 88. Perfect drainage time treatment after 40~50 days was 3% higher in percent ripened grain and 0.6 g heavier in 1,000 grain weight than treatment after 25~35 days. There was no difference of rice yield between perfect drainage time treatment after 25 days and 30~35 days, but rice yield was 7~8% higher in treatment after 40~50 days than 25 days. Head rice ratio in treatment after 35 days was the highest and the sooner perfect drainage time, the lower protein content. Soil moisture negatively correlated with soil hardness and EC in this result. With this results, we proposed that the time of perfect drainage in newly reclaimed land to stable rice production is 40~50 days after heading date.

• Korean Journal of Soil Science and Fertilizer
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• v.41 no.5
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• pp.318-323
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• 2008

We studied on the effects of slurry composting and biofiltration liquid fertilizer (SCBLF) on growth of two-year-old poplar clones planted in a reclaimed land mounding soil. The soil on the experimental site had lower concentrations of both exchangeable cations and salinity than before reclamation. However, the content of organic matter was low compared with the most soils. We applied SCBLF to the poplars six times and 5 L in each time for 80 days. Ten clones of six poplar species or hybrids were tested in this study: Populus alba ${\times}$ P. glandulosa(Clivus, 72-30, 72-31, Bongwha1), P. deltoides ${\times}$ P. nigra(Dorskamp), P. deltoides(Lux) ${\times}$ P. deltoides(Harvard)(97-19), P. euramericana(Eco28, I-476), P. nigra ${\times}$ P. maximowiczii(62-2) and P. Koreana ${\times}$ P. nigra var. italica(Suwon). Growth performance varied more among clones than among species. Average height growth of treated plots was 18% greater than control, and clones Clivus, 97-19, Eco28 and Dorskamp were more vigorous than other clones. Diameter at breast height in treated plots was 41% greater than control, and 97-19, Dorskamp, Eco28 and Clivus were the four best clones in this respect. Mean leaf area of treated trees was 26% greater for control trees. Chlorophyll content was similar between treated and controlled trees. Total nitrogen values in leaves were much higher in treated trees 18%. SCBLF treatment on poplars planted in reclaimed land helped tree growth.