• Information Systems Review
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• v.4 no.2
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• pp.191-208
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• 2002

In the area of new millennium of twenty first century, one of the urgent and critical research issues in commerce area is the regenerating of new business opportunities from the high value added perspectives. With this motivation, in this paper, we create new on-line e-businesses with the speed of lightning their affiliated supply, finance and business communities - which are involved in more and more tightly connected, open trading - we find that we need to deal with hundreds of business collaborative partners, millions of buyers and sellers while we have to face incompatible IT systems. Challenges of new business opportunities linking multi-enterprise data and processes cost effectively, reliably and securely in real time remains an open area in e-business. This challenge we shall describe as the problem of synchronization of multiple enterprise collaborative e-business opportunities (production related), value (finance related), business (operations related) in new business opportunity and infrastructure integrated all together over the off-line and online basis. It brings a new e-commerce opportunities infrastructure into this profitable challenge: by extracting and tracking new business information, new trends in the events of e-business processes. The transformation of the traditional commerce into this type of electronic based commerce can be interpreted as new Cultural Revolution. The revolution will be a new paradigm crossing over the geographical, and organizational zone, restructuring enterprise business process infrastructure.

• Journal of Internet Computing and Services
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• v.18 no.2
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• pp.21-30
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• 2017

IoT(Internet of Things) can enable networking and computing using any devices is rapidly proliferated. In the existing IoT environment, bottlenecks and service delays can occur because it processes data and provides services to users using central processing based on Cloud. For this reason, Edge Computing processes data directly in IoT nodes and networks to provide the services to the users has attracted attention. Also, numerous researchers have been attracted to intelligent service efficiently based on Edge Computing. In this paper, expert system-based context awareness scheme for Edge Computing in IoT environment is proposed. The proposed scheme can provide customized services to the users using context awareness and process data in real-time using the expert system based on efficient cooperations of resource limited IoT nodes. The context awareness services can be modified by the users according to the usage purpose. The three service modes in the security system based on smart home are used to test the proposed scheme and the stability of the proposed scheme is proven by a comparison of the resource consumptions of the servers between the proposed scheme and the PC-based expert system.

• Food Science of Animal Resources
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• v.32 no.5
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• pp.678-684
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• 2012

The melanin pigment in human skin is a major defense mechanism against ultraviolet light to the skin, but darken skin color. Tyrosinase is mainly responsible for melanin biosynthesis (melanogenesis) in animals and enzymatic browning (melanosis) in plants. The purpose of this study was to optimize the fermented milk process for the melanin formation inhibition by using Lactobacillus plantarum M23 with tyrosinase inhibitory activity. We used 4-factor-3-level central composite design combining with response surface methodology. Yeast extract concentration (%, $X_1$), addition of grape (%, $X_2$), incubation temperature ($^{\circ}C$, $X_3$) and incubation time (h, $X_4$) was used as an independent factor, on the other hand, pH (pH, $Y_1$), overall palatability (score, $Y_2$) and tyrosinase inhibitory activity (%, $Y_3$) was used as a dependant factor. Based on the optimization for the highest tyrosinase inhibitory activity with pH 4.4, the expected data of pH, palatability and tyrosinase inhibitory activity with 14.8 h incubation at $37.1^{\circ}C$ by the addition of 0.127% of yeast extract, 2.95% of grape was 4.42, 7.06 and 86.65%, but the real data was 4.35, 6.86 and 84.05%, respectively. Based on the previous results, fermented milk using Lactobacillus plantarum M23 with the tyrosinase inhibitory activity could contribute for the whitening and antiaging of human skin.

• Journal of Korean Society of Rural Planning
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• v.23 no.3
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• pp.163-174
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• 2017

This study developed the prototype of the system and implemented its main functions, which is the intelligent integrated agricultural water management information system and service (IaWAMISS). The developed system was designed to be able to collect, process and analyze the agricultural water information of spatially dispersed reservoirs in whole country and spatial geographic information distributed in various systems of other organizations. The system, IaWAMISS, is also possible to provide the reproduced information services in each reservoir and space units, such as agricultural water demand and supply analysis and drought prediction, to the people, experts, and policy makers. This study defined the 6 step modules to develop the system, which are to design the components of intelligent integrated information system, to derive the utilization contents of existing systems, to design the new development elements for IaWAMISS, to design the reservoir information system can be used by managers of city and county, to designate the monitoring reservoirs managed by city and county, and finally to prepare the sharing system between organizations with the existing information systems. In order to implement the prototype of the system, this study shows the results for three important functions of the system: spatial integration of reservoirs' information, data link integration between the existing systems, and intelligent analysis program development to assist decision support for agricultural water management. For the spatial integration with the reservoir water information of the Korea Rural Community Corporation, this study get IaWAMISS to receive the real-time reservoir storage information from the measurement facility installed in the municipal management reservoir. The data link integration connecting databases of the existing systems, was implemented by integrating the meteorological information of the Korea Meteorological Administration with IaWAMISS, so that the rainfall forecast data could be derived and used. For the implementation of the intelligent analysis program, this study also showed the results of analysis and prediction of agricultural water demand and supply amount, estimation of Palmer drought index, analysis of flood risk area in typhoon course region, and analysis of the storage status of reservoirs related to each storm. This study confirmed the possibility and efficiency of an useful system development through the prototype design and implementation of IaWAMISS. By solving the preliminary 6 step modules presented in this study, it is possible not only to efficiently manage water by spatial unit, but also to provide the service of information and to enhance the relevant policy and national understanding to the people.

• Korean Journal of Veterinary Service
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• v.39 no.2
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• pp.107-116
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• 2016

In the study, we developed and evaluated a uracil N-glycosylase (UNG)-supplemented single-tube nested reverse transcription-polymerase chain reaction (UsnRT-PCR) assay that can carried out first-round RT-PCR and second-round nested PCR in a reaction tube without reaction tube opening and can simultaneously detect EU- and NA-PRRSV. The UsnRT-PCR confirmed to have a preventing ability of mis-amplification by contamination of pre-amplified PRRSV DNA from previous UsnRT-PCR. Primer specificities were evaluated with RNAs extracted from 8 viral strains and our results revealed that the primers had a high specificity for both genotypes of PRRSV. The sensitivity of the UsnRT-PCR was 0.1 $TCID_{50}$/0.1 mL for EU- or NA-PRRSV, respectively, which is comparable to that of previously reported real time RT-PCR (RRT-PCR). Clinical evaluation on 110 field samples (60 sera and 50 lung tissues) by the UsnRT-PCR and the RRT-PCR showed that detection rates of the UsnRT-PCR was 70% (77/110), and was relatively higher than that of the RRT-PCR (69.1%, 76/110). The percent positive or negative agreement of the UsnRT-PCR compared to RRT-PCR was 96.1% (73/76) or 90.9% (30/33), showing that the test results of both assays may be different for some clinical samples. Therefore, it is recommend that diagnostic laboratory workers use the two diagnostic assays for the correct diagnosis for the relevant samples in the swine disease diagnostic laboratories. In conclusion, the UsnRT-PCR assay can be applied for the rapid, and reliable diagnosis of PRRSV without concerns about preamplified DNA carryover contamination that can occurred in PCR process in the swine disease diagnostic laboratories.

• Journal of Embryo Transfer
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• v.25 no.2
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• pp.103-110
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• 2010

The first morphogenetic event of preimplantation development, compaction, was required efficient production of porcine embryos in vitro. Compaction of the porcine embryo, which takes place at post 4-cell stage, is dependent upon the adhesion molecule E-cadherin. The E-cadherin through ${\beta}$-catenin contributes to stable cell-cell adhesion. Rho-associated kinase (ROCK) signaling was found to support the integrity of E-cadherin based cell contacts. In this study, we traced the effects of ROCK-1 on early embryonic development and structural integrity of blastocysts in pigs. Then, in order to gain new insights into the process of compaction, we also examined whether ROCK-1 signaling is involved in the regulation of the compaction mediated by E-cadherin of cellular adhesion molecules. As a result, real-time RT-PCR analysis showed that the expression of ROCK-1 mRNA was presented throughout porcine preimplantation stages, but not expressed as consistent levels. Thus, we investigated the blastocyst formation of porcine embryos treated with LPA and Y27632. Blastocysts formation and their qualities in LPA treated group increased significantly compared to those in the Y27632-treated group (p < 0.05). Then, to determine whether ROCK-1 associates embryonic compaction, we explored the effect of activator and/or inhibitor of ROCK-1 on compaction of embryos in pigs. The rate of compacted morula in LPA treated group was increased compared to that in the Y27632-treated group (39.7 vs 12.0%). Furthermore, we investigated the localization and expression pattern of E-cadherin at 4-cell stage porcine embryos in both LPA- and Y27632-treated groups by immunocytochemical analysis and Western blot analysis. The expression of E-cadherin was increased in LPA-treated group compared to that in the Y27632-treated group. The localization of E-cadherin in LPA-treated group was enriched in part of blastomere contacts compared to that Y27632-treated group. ROCK-1 as a crucial mediator of embryo compaction may plays an important role in regulating compaction through E-cadherin of the cell adhesion during the porcine preimplantation embryo. We concluded that ROCK-1 gene may affect the developmental potential of porcine blastocysts through regulating embryonic compaction.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.30 no.2
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• pp.211-221
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• 2012

To utilize omni-directional images acquired by a rotating line camera for indoor spatial information services, we should register precisely the images with respect to an indoor coordinate system. In this study, we thus develop a georeferencing method to estimate the exterior orientation parameters of an omni-directional image - the position and attitude of the camera at the acquisition time. First, we derive the collinearity equations for the omni-directional image by geometrically modeling the rotating line camera. We then estimate the exterior orientation parameters using the collinearity equations with indoor control points. The experimental results from the application to real data indicate that the exterior orientation parameters is estimated with the precision of 1.4 mm and $0.05^{\circ}$ for the position and attitude, respectively. The residuals are within 3 and 10 pixels in horizontal and vertical directions, respectively. Particularly, the residuals in the vertical direction retain systematic errors mainly due to the lens distortion, which should be eliminated through a camera calibration process. Using omni-directional images georeferenced precisely with the proposed method, we can generate high resolution indoor 3D models and sophisticated augmented reality services based on the models.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.8
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• pp.5513-5521
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• 2015

There is an operational efficiency problem about wayside equipment applied to the domestic low-density branch as the equipment has been installed and operated similarly in the mainline. On-board oriented train control system, which has been developed for train safety and operation efficiency, ensures safe train operation without expensive ground control signal devices. Such system consists of on-board control system, wayside control system, and local control system. In this paper, the details of tests such as suitability test, communication test, and interface test are described by installing the on-board control system and wayside control system in field. Installation tests include checking power, voltage, cable connection, LED status, etc. Field applicability of the developed system is also verified through the dynamic operation tests with diverse scenarios, which are performed on the virtual line similar to the real environment including switch machine and level crossing gate. Dynamic operation tests were conducted for total 7 scenarios, and several tests were repeated for each scenario. The elapsed time for each operation was computed by analyzing main process log, and we could check that each operation was accomplished within several seconds. Furthermore, the developed system was verified through field test with an accredited institute, and testing certificates were issued.

• Journal of Elementary Mathematics Education in Korea
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• v.20 no.2
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• pp.193-213
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• 2016

This study has two goals. The First is to develop and apply a step-by-step program and the degree to which students' mathematical skills. The second is to analyze mathematical attitude change around the first grade students was done. The program for learning complement number is composed of series of 5 steps and 11 classes. Play for learning complement number, taking into account the difficulty of the learning steps 1-5 are organized. First step is composed of the classes which fragmented pieces of shapes to complete the entire geometry with fun activities for the entire part of the concept of learning and it maintenance concepts and can naturally learn by associating step. In second step, tools to take advantage of the real world and collecting the conservative concept. 3rd steps is to repair the mathematical concept of the parish in the learning stage of expansion. 4th step is halrigalri, number cards, making ten games etc. 5th step is to verify the concept of complement number and number operation ability. The concept of complement number through fun activities can improve students' mathematical skills, and mathematical attitude change. Early in the program, students use the finger to throw acid in the process. Simple addition and subtraction calculations may take a long time and error, but more and more we progress through the program using the fingers is eliminated and a more complex form calculations was not difficult to act out.

• Korean Journal of Clinical Laboratory Science
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• v.52 no.1
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• pp.53-61
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• 2020

Cell migration is a central process for recovering from wounds triggered by physical distress besides embryogenesis and cancer metastasis. Wound healing assay is widely used as a fundamental research technique for investigation of two-dimensional cell migration in vitro. The most common approach for imitating physical wound in vitro is mechanical scratching on the surface of the confluent monolayer by using sharp materials. The iron metal pin with a suspension spring for fine adjustment of the orthogonal contact surface between the scratching point and the individual bottom of multi-well plate with planar curvatures were adopted for the creative invention of a 96-well plate wound maker. While classic tips drew diverse and zigzag scratching patterns on the confluent monolayer, our wound maker displayed synchronized linear wounds in the middle of each well of a 96-well plate that was seeded with several cell lines. Given that several types of multi-well plates commercially available are compatible with our lab-made wound maker for creating uniform scratches on the confluent monolayer for the collective cell migration in wound healing assay, it is certain that the application of this wound maker to the real-time wound healing assay in high content screening (HCS) is superior than utilization of typical polypropylene pipette tips.