• Journal of Korean Society for Geospatial Information Science
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• v.12 no.4 s.31
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• pp.53-60
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• 2004

Congestion index is needed for quantifying congestion level for various areas. So far, the index has been calculated based on multiple vehicle data for specified time interval. Such being the case, it was costly to build it and the usage of it was focused on policy development and evaluation rather than on traffic information provision. This study focuses on a development on a single vehicle based congestion index which can be a representative value for link congestion level and link speed information at the same time for dual purposes of traditional usages and information provision. A new term has been added for representing real time based arterial congestion level and it has been verified on a real time basis. The index was based on single vehicle GPS data and seemed to be cost effective in deriving the index. With the help of the index, the traffic information contents can be diversified in a constructive way in providing real time traffic information for ITS area and in using congestion level determination for traditional transportation areas.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.42 no.1
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• pp.67-74
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• 2022

In recent, the number of real-time traffic information sources and providers has increased as increasing smartphone users and intelligent transportation system facilities installed at roadways including vehicle detection system (VDS), dedicated short-ranged communications (DSRC), and global positioning system (GPS) probe vehicle. The accuracy of such traffic information would vary with these heterogeneous information sources or spatiotemporal traffic conditions. Therefore, the purpose of this study is to propose an empirical strategy of heterogeneous information fusion to improve the accuracy of real-time traffic information. To carry out this purpose, travel speed data collection based on the floating car technique was conducted on 227 freeway links (or 892.2 km long) and 2,074 national highway links (or 937.0 km long). The average travel speed for 5 probe vehicles on a specific time period and a link was used as a ground truth measure to evaluate the accuracy of real-time heterogeneous traffic information for that time period and that link. From the statistical tests, it was found that the proposed fusion strategy improves the accuracy of real-time traffic information.

• Journal of Microbiology and Biotechnology
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• v.22 no.2
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• pp.248-255
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• 2012

In order to develop a protocol to quantify cyanobacteria and Microcystis simultaneously, the primers and probe were designed from the conserved regions of 16S rRNA gene sequences of cyanobacteria and Microcystis, respectively. Probe match analysis of the Ribosomal Database Project showed that the primers matched with over 97% of cyanobacterial 16S rRNA genes, indicating these can be used to amplify cyanobacteria specifically. The TaqMan probe, which is located between two primers, matched with 98.2% of sequences in genus GpXI, in which most Microcystis strains are included. The numbers of cyanobacterial genes were estimated with the emission of SYBR Green from the amplicons with two primers, whereas those of Microcystis spp. were measured from the fluorescence of CAL Fluor Gold 540 emitted by exonuclease activity of Taq DNA polymerase in amplification. It is expected that this method enhances the accuracy and reduces the time to count cyanobacteria and potential toxigenic Microcystis spp. in aquatic environmental samples.

• Journal of the Optical Society of Korea
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• v.18 no.2
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• pp.151-155
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• 2014

Although various ophthalmic imaging methods, including fundus photography and optical coherence tomography, have been applied for effective diagnosis of ocular diseases with high spatial resolution, most of them are limited by shallow imaging penetration depth and a narrow field of view. Also, many of those imaging modalities are optimized to provide microscopic anatomical information, while functional or cellular information is lacking. Compared to other ocular imaging modalities, photoacoustic imaging can achieve relatively deep penetration depth and provide more detailed functional and cellular data based on photoacoustic signal generation from endogenous contrast agents such as hemoglobin and melanin. In this paper, array-based ultrasound and photoacoustic imaging was demonstrated to visualize pigmentation in the eye as well as overall ocular structure. Fresh porcine eyes were visualized using a real-time ultrasound micro-imaging system and an imaging probe supporting laser pulse delivery. In addition, limited photoacoustic imaging field of view was improved by an imaging probe tilting method, enabling visualization of most regions of the retina covered in the ultrasound imaging.

• KIEE International Transactions on Electrophysics and Applications
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• v.11C no.3
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• pp.85-90
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• 2001

The determination of DNA hybridization reaction can apply the molecular biology research, clinic diagnostics, bioengineering, environment monitoring, food science and other application area. So, the improvement of DNA detection system is very important for the determination of this hybridization reaction. In this study, we report the characterization of the probe and target oligonucleotide hybridization reaction using the evanescent field microscopy. First, we have fabricated DNA chip microarray. The particles which were immobilized oligonucleotides were arranged by the random fluidic self-assembly on the pattern chips, using hydrophobic interaction. Second, we have detected DNA hybridization reaction using evanescent field microscopy. The 5'-biotinylated probe oligonucleotides were immobilized on the surface of DNA chip microarray and the hybridization reaction with the Rhodamine conjugated target oligonucleotide was excited fluorescence generated on the evanescent field microscopy. In the foundation of this result, we could be employed as the basis of a probe olidonucleotide, capable of detecting the target oligonucleotide and monitoring it in a large analyte concentration range and various mismatching condition.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.19 no.1
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• pp.203-209
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• 2019

In this work, a new land-specific resistance measuring device (GM) and a measuring probe (Grounding Rod) are connected to the WENNER quadrant as power-line communication (PLC). In groups of two (P1,P2) probes, five to ten probes are installed in series on the ground at intervals of 1m, 2m, 4m, 8m, and 16m, respectively. If the PLC signal from the GMD is detected by the receiver of the Probe 1 (P1) for measurement, the minute voltage and current for measurement flow from the PSD (power supply) attached to the probe to the ground, and then, through the soil between P1 and P2, enters the Probe 1 (P2). The resistance value is then measured by the principle of voltage drop due to ground resistance. Measure the earth resistance every T seconds up to 1 trillion and store the measured data on the Arduino Server mounted on the main equipment. Stored measurement data can be derived from formulas by Ohm's Law and from inherent resistance (here,). Data obtained in real time will be linked to CDGES programs installed on Main PC, enabling data analysis and real-time monitoring of the ground environment on land. In addition, a three-dimensional display is possible with 3D graph support by identifying seasonal characteristics such as temperature and humidity of land (soils). The limitations of the study will require specific application measures of Test Bed for commercial access to a model that has been developed and operated experimentally.

• Journal of Advanced Marine Engineering and Technology
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• v.33 no.6
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• pp.925-931
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• 2009

This paper reports a preliminary experimental investigation and characterization of an optical fiber-based submersion sensor system for applications in water flooding and leakage. The sensor system comprises a multi-mode fiber spliced with fiber Bragg grating and an intensity-based interrogator. Submersion tests were conducted in water-air and Glycerin-air environments. By the refractive index of the fiber-probe surrounding materials, the reflectance and the detecting power level is determined. When the probe is dipped into the water, the optical output power dramatically decreases from -7.5dBm to -17.5dBm. But, the center of Bragg wavelength is not affected in spite of external material changes. Temporal response characteristics of the sensor system is investigated to verify the real-time reaction. When the probe is immersed into the liquid, there is no transition time.

• Journal of Microbiology and Biotechnology
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• v.18 no.8
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• pp.1453-1458
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• 2008

For quantitative PCR assay of Salmonella enterica serovar Typhimurium in food samples, a real-time PCR method was developed, based on DNA genome equivalent. Specific primers and probe designed based on the STM4497 gene of S. Typhimurium LT2 showed the specificity to S. Typhimurium. Threshold cycle (Ct) values of real-time PCR were obtained from a quantitative standard curve with genomic DNA of Salmonella Typhimurium. In addition, the recovery of S. Typhimurium inoculated artificially to chicken samples with $4.5{\times}10^5$ to 4.5 CFU/ml was evaluated by using real-time PCR and plate-count methods. Result showed that the number of cells calculated from the real-time PCR method had good correlation with that of the plate-count method. This real-time PCR method could be applicable to the detection and quantification of S. Typhimurium in food samples.

• Biomedical Science Letters
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• v.16 no.2
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• pp.127-131
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• 2010

Mycobacterium leprae detection is difficult even with molecular biological techniques due to the low sensitivity of current methodologies. In this report, real-time PCR targeting the M. leprae-specific repetitive element (RLEP) sequence was developed as a new diagnostic tool and evaluated using clinical specimens. For this, M. leprae DNAs were extracted from skin biopsy specimens from 80 patients and analyzed by real-time PCR using TaqMan probe. Then, the detection efficiency of the real-time PCR was compared with that of standard PCR. In brief, the rate of positive detection by the standard PCR and real-time PCR was 32.50% and 66.25%, respectively. The results seemed to clearly show that the TaqMan real-time PCR developed in this study may be a useful tool for sensitive detection of M. leprae from clinical specimens.

• Journal of the Semiconductor & Display Technology
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• v.19 no.3
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• pp.43-48
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• 2020

The demand for stable plasma has been on the rise because of the increased delivery power amount in the chamber for improving productivity, and fast and accurate plasma impedance matching become a crucial performance measure for radio frequency (RF) power system in semiconductor manufacturing equipment. In this paper, the overall impedance matching was understood, and voltage and current values were extracted with voltage - current (VI) probe to measure plasma impedance in real-time. Actual matching data were analyzed to derive calibration coefficient for V and I measurements to understand the characteristics of VI probe, and we demonstrated the tendency of RF impedance matching according to changes in load impedance. This preliminary empirical research can contribute to fast RF matching as well as advanced equipment control for the next level of detailed investigation on embedded system based-RF matching controller.