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Direct and Quantitative Analysis of Salmonella enterica Serovar Typhimurium Using Real-Time PCR from Artificially Contaminated Chicken Meat  

Park, Hee-Jin (Institute of Life Sciences and Resources, and Graduate School of Biotechnology, Kyung Hee University)
Kim, Hyun-Joong (Institute of Life Sciences and Resources, and Graduate School of Biotechnology, Kyung Hee University)
Park, Si-Hong (Institute of Life Sciences and Resources, and Graduate School of Biotechnology, Kyung Hee University)
Shin, Eun-Gyeong (Institute of Life Sciences and Resources, and Graduate School of Biotechnology, Kyung Hee University)
Kim, Jae-Hwan (Institute of Life Sciences and Resources, and Graduate School of Biotechnology, Kyung Hee University)
Kim, Hae-Yeong (Institute of Life Sciences and Resources, and Graduate School of Biotechnology, Kyung Hee University)
Publication Information
Journal of Microbiology and Biotechnology / v.18, no.8, 2008 , pp. 1453-1458 More about this Journal
Abstract
For quantitative PCR assay of Salmonella enterica serovar Typhimurium in food samples, a real-time PCR method was developed, based on DNA genome equivalent. Specific primers and probe designed based on the STM4497 gene of S. Typhimurium LT2 showed the specificity to S. Typhimurium. Threshold cycle (Ct) values of real-time PCR were obtained from a quantitative standard curve with genomic DNA of Salmonella Typhimurium. In addition, the recovery of S. Typhimurium inoculated artificially to chicken samples with $4.5{\times}10^5$ to 4.5 CFU/ml was evaluated by using real-time PCR and plate-count methods. Result showed that the number of cells calculated from the real-time PCR method had good correlation with that of the plate-count method. This real-time PCR method could be applicable to the detection and quantification of S. Typhimurium in food samples.
Keywords
Real-time PCR; Salmonella Typhimurium; quantitative analysis;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
Times Cited By Web Of Science : 3  (Related Records In Web of Science)
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