• The Korean Journal of Food And Nutrition
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• v.31 no.2
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• pp.284-292
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• 2018

This study was conducted to determine the elements that are required and that need improvement in the contents of dietary life education and the educational environment by analyzing the importance-satisfaction of a dietary life education program for dietary life education trainees. The results of the rank test for the satisfactory elements of dietary life education were in the order of competence of instructors, educational contents, time of education, educational environment and affordability of tuition. The importance-satisfaction analysis of the contents and construction of the dietary life education program showed that "suitable contents for the theme of the class", "contents and construction of practice class", "diversity of practice menu" and "diversity of educational contents" had high importance and satisfaction scores and therefore, could be maintained at their current levels. Meanwhile, "adequacy of class time" and "contents and construction of theory class" showed low importance and satisfaction scores, requiring a little effort. Whereas, "time and construction of group practice", "structure of textbook for class" and "duration of course and number of classes" showed relatively low satisfaction compared to importance, requiring major efforts for improvement. Similarly, analysis of the lecture element from dietary life education showed that "professional lecture by instructors", "lectures suitable for themes" and "lecture applicable in real life" had relatively low satisfaction compared to importance, requiring major efforts for improvement. In addition, analysis of the educational environment from dietary life education showed that "adequacy of lecture room space", "cooking equipment", "diversity of cooking utensils" and "sanitary condition of cooking utensils" also require major efforts for improvement. Therefore, dietary life education should not be executed with a simple delivery of knowledge but with both theory and practice classes to increase its application in real life, and active and specific efforts are required to nurture professional instructors and establish educational strategies.

• Journal of Institute of Control, Robotics and Systems
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• v.21 no.12
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• pp.1100-1108
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• 2015

A camera-based real-time hand posture and gesture recognition system is proposed for controlling various devices inside automobiles. It uses an imaging system composed of a camera with a proper filter and an infrared lighting device to acquire images of hand-motion sequences. Several steps of pre-processing algorithms are applied, followed by a background normalization process before segmenting the hand from the background. The hand posture is determined by first separating the fingers from the main body of the hand and then by finding the relative position of the fingers from the center of the hand. The beginning and ending of the hand motion from the sequence of the acquired images are detected using pre-defined motion rules to start the hand gesture recognition. A set of carefully designed features is computed and extracted from the raw sequence and is fed into a decision tree-like decision rule for determining the hand gesture. Many experiments are performed to verify the system. In this paper, we show the performance results from tests on the 550 sequences of hand motion images collected from five different individuals to cover the variations among many users of the system in a real-time environment. Among them, 539 sequences are correctly recognized, showing a recognition rate of 98%.

• Proceedings of the KIEE Conference
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• 1996.07b
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• pp.1398-1400
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• 1996

In this paper, a method of the decision of the road direction for ALV(Autonomous Land Vehicle) road following by region-based segmentation is presented. The decision of the road direction requires extracting road regions from images in real-time to guide the navigation of ALV on the roadway. Two thresholds to discriminate between road and non-road region in the image are easily decided, using knowledge of problem region and polygonal approximation that searches multiple peaks and valleys in histogram of a road image. The most likely road region of the binary image is selected from original image by these steps. The location of a vanishing point to indicate the direction of the road can be obtained applying it to X-Y profile of the binary road region again. It can successfully steer a ALV along a road reliably, even in the presence of fluctuation of illumination condition, bad road surface condition such as hidden boundaries, shadows, road patches, dirt and water stains, and unusual road condition. Pyramid structure also saves time in processing road images and a real-time image processing for achieving navigation of ALV is implemented. The efficacy of this approach is demonstrated using several real-world road images.

• Journal of People, Plants, and Environment
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• v.22 no.2
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• pp.127-143
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• 2019

This study investigates the color stimuli of two varieties of foliage plants by extracting electroencephalogram, electrocardiogram and physiology activity data from 30 participants in their 50s or older. Changes in the physiological activity of subjects against six color stimuli were examined. The stimulus to real green plants 'Silver Queen' was set as the control group, and was compared with other groups including the stimulus to real 'Angel' plants and four stimuli to artificial colors (two color images and color schemes of the same green and red plants). Compared to the five groups, the relative theta power spectrum (RT) and the ratio of alpha to high beta (RAHB) increased in the subjects exposed to real green plants. This result demonstrates that the green plant ('Silver Queen') increases the stability, relaxation, and internal concentration of subjects in a proper state of awakening. The result of this experiment showed a statistically significant difference in the level of RT when subjects were exposed to the groups of real green and red plants. This finding indicates that the green plant increases internal concentration more than the red plant. RT and the relative low beta power spectrum (RLB) in the groups of natural colors were higher than the groups of artificial colors when subjects focused their mind on the two types of real plants. However, the level of relative mid beta power spectrum (RMB), ratio of SMR to theta (RST), ratio of mid beta to theta (RMT), relative high beta power spectrum (RHB), and spectral edge frequency 95% were higher when subjects were exposed to the photos and colors scheme of plants than when they were exposed to real plants. The subjects experienced more "comfortable" emotions when they were looking at plants with green colors. Overall, it is recommended to use the natural colors of real plants in places where which stability and relaxation are required. On the contrary, the artificial colors of plants such as their photos and color schemes are useful in places where a high level of concentration is required in a short period of time.

• Korean Journal of Community Nutrition
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• v.16 no.6
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• pp.697-705
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• 2011

This study was conducted to investigate the effects of real time communication digital photography method using Kakao Talk application in smart-phone for the fortification of food consumption monitoring and weight reduction. Thirty-four female college students were randomly assigned to the camera-phone (CP) group or smart-phone (SP) group. Each group participated in the weight control program for 8 weeks. The mean energy intake of CP group during program was 1353.5 kcal and the SP group consumed 1289.2 kcal. The total energy intake of both groups was significantly decreased during the program. The CP group lost 1.9 kg of body weight and 1.9% of body fat and the SP group lost 4.3 kg of body weight and 3.0% of body fat. The body weight was significantly decreased in the SP group compared to the CP group. The triglyceride and total cholesterol, and LDL-cholesterol level of SP group were significantly decreased during the program. However, there were no significant changes in CP group during the program. Also there were no significant changes in lipid profile between two groups. In this study, it is considered that real time communication digital photography method using Kakao Talk application in smart-phone might influence weight control through a trained consumption monitoring. Therefore, smart-phone can lead individuals to rely more heavily on easy-to-monitor visual cues.

• Journal of Veterinary Science
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• v.22 no.3
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• pp.40.1-40.12
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• 2021

Background: The microsporidian parasite Nosema ceranae is a global problem in honeybee populations and is known to cause winter mortality. A sensitive and rapid tool for stable quantitative detection is necessary to establish further research related to the diagnosis, prevention, and treatment of this pathogen. Objectives: The present study aimed to develop a quantitative method that incorporates ultra-rapid real-time quantitative polymerase chain reaction (UR-qPCR) for the rapid enumeration of N. ceranae in infected bees. Methods: A procedure for UR-qPCR detection of N. ceranae was developed, and the advantages of molecular detection were evaluated in comparison with microscopic enumeration. Results: UR-qPCR was more sensitive than microscopic enumeration for detecting two copies of N. ceranae DNA and 24 spores per bee. Meanwhile, the limit of detection by microscopy was 2.40 × 10⁴ spores/bee, and the stable detection level was ≥ 2.40 × 10⁵ spores/bee. The results of N. ceranae calculations from the infected honeybees and purified spores by UR-qPCR showed that the DNA copy number was approximately 8-fold higher than the spore count. Additionally, honeybees infected with N. ceranae with 2.74 × 10⁴ copies of N. ceranae DNA were incapable of detection by microscopy. The results of quantitative analysis using UR-qPCR were accomplished within 20 min. Conclusions: UR-qPCR is expected to be the most rapid molecular method for Nosema detection and has been developed for diagnosing nosemosis at low levels of infection.

• Journal of Veterinary Clinics
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• v.25 no.6
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• pp.435-439
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• 2008

We evaluated the patterns of serology and fecal viral shedding for any differences by hemagglutination inhibition (HI) and real-time PCR on Korean CPV-2 isolates (CPV-2a-I, CPV-2a-V and CPV-2b). We successfully detected fecal viral shedding from samples extracted 2-3 d.p.i., regardless of the onset of clinical signs. In addition, the pattern of viral shedding differed depending on the CPV-2 isolates used for inoculation. We also observed differences in the serological pattern that was also depended on the CPV-2 isolates inoculated. The onset and amount of fecal viral shedding were not correlated with the level of antibody titers in this study. Our study is a valuable resource for understanding the different pathobiology of the CPV-2 isolates and the correlation between the patterns of serum antibody titer and fecal viral shedding.

• Journal of Pharmaceutical Investigation
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• v.37 no.6
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• pp.329-338
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• 2007

The quality assurance issue of drug products is more important than the general product because it is highly related to the human health and life. In this reason, the regulatory guide lines have continuously been intensified all around the world. In order to achieve effective quality assurance and real-time product release (RTPR) of drug products, process analytical technology (PAT), which can analyze and control a manufacturing process, has been proposed from the United States. With the PAT process, we can obtain significant process features of materials, quality characteristics and product capabilities from a raw material to the final product in the real-time procedure. PAT can also be utilized to process validation using information system that can analyze the risk of drug products through out an entire product life-cycle. In this paper, we first offered a new concept for the off-line process design methods to prepare the improved quality assurance restrictions and a real-time control method by establishing an information system. We also introduced an automatic inspection system by obtaining surrogate variables based on drug product formulations. Finally, we proposed an advanced PAT concept using validation and feedback principles through out the entire life-cycle of drug product manufacturing processes.

• Journal of Microbiology and Biotechnology
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• v.15 no.3
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• pp.595-602
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• 2005

A one-step real-time quantitative RT-PCR assay in combination with automated RNA extraction was evaluated for routine testing of HCV RNA in the laboratory. Specific primers and probes were developed to detect 302 bp on 5'-UTR of HCV RNA. The assay was able to quantitate a dynamic linear range of $10^7-10^1$ HCV RNA copies/reaction ($R^2=0.997$). The synthetic HCV RNA standard of $1.84{\pm}0.1\;(mean{\pm}SD)$ copies developed in this study corresponded to 1 international unit (IU) of WHO International Standard for HCV RNA (96/790 I). The detection limit of the assay was 3 RNA copies/reaction (81 IU/ml) in plasma samples. The assay was comparable to the Amplicor HCV Monitor (Monitor) assay with correlation coefficient r=0.985, but was more sensitive than the Monitor assay. The assay could be completed within 3 h from RNA extraction to detection and data analysis for up to 32 samples. It allowed rapid RNA extraction, detection, and quantitation of HCV RNA in plasma samples. The method provided sufficient sensitivity and reproducibility and proved to be fast and labor-saving, so that it was suitable for high throughput HCV RNA test.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.22 no.11
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• pp.1531-1537
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• 2018

In maritime logistics, a large number of containers are transported to the destination through a port in the form of composite transportation such as ships, trucks, and railways. Logistics parties such as shipping companies and consignors prefer having the real-time information on the entry and exit of the logistics hubs of the freight. Particularly, a port is an important space that connects the maritime and inland areas. Hence, the interest about freight of the logistics party in port is high. This study describes tracking the location of freight by using GPS dynamically based on area type rather than point type. This presents dynamic information of location tracking when the freight enters, exits from, and passes through a certain port area. I suggest a real-time location of the container cargos tracking algorithm in port-entry, port-exit, and port inside using Active IP-RFID technology based on the location tracking algorithm of previous research.