• Tuberculosis and Respiratory Diseases
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• v.70 no.6
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• pp.462-473
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• 2011

Background: Smoking is a risk factor for idiopathic pulmonary fibrosis (IPF), but the mechanism of the association remains obscure. There is evidence demonstrating that plasminogen activator inhibitor-1 (PAI-1) is involved in the progression of pulmonary fibrosis. This study was to determine whether the administration of small interfering RNA (siRNA) targeting PAI-1 or PAI-1 inhibitor to the cigarette smoking extract (CSE)-exposed rat alveolar type II epithelial cells (ATII cells) limits the epithelial-mesenchymal transition (EMT). Methods: ATII cells were isolated from lung of SD-rat using percoll gradient method and cultured with 5% CSE. The EMT was determined from the ATII cells by measuring the real-time RT PCR and western blotting after the PAI-1 siRNA transfection to the cells and after administration of tiplaxtinin, an inhibitor of PAI-1. The effect of PAI-1 inhibitor was also evaluated in the bleomycin-induced rats. Results: PAI-1 was overexpressed in the smoking exposed ATII cells and was directly associated with EMT. The EMT from the ATII cells was suppressed by PAI-1 siRNA transfection or administration of tiplaxtinin. Signaling pathways for EMT by smoking extract were through the phosphorylation of SMAD2 and ERK1/2, and finally Snail expression. Tiplaxtinin also suppressed the pulmonary fibrosis and PAI-1 expression in the bleomycin-induced rats. Conclusion: Our data shows that CSE induces rat ATII cells to undergo EMT by PAI-1 via SMAD2-ERK1/2-Snail activation. This suppression of EMT by PAI-1 siRNA transfection or PAI-1 inhibitor in primary type II alveolar epithelial cells might be involved in the attenuation of bleomycin-induced pulmonary fibrosis in rats.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.40 no.12
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• pp.27-37
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• 2003

In the next generation Internet(NGI) based on dense wavelength division multiplexing(DWDM) technology, QoS RWA considering various QoS parameters of DWDM networks is regard as one of the key issues in providing real-time multimedia services. However, finding a qualified path meeting multi-constraints is generally NP-complete problem. It is insufficient for QoS RWA researches in DWDM networks that must consider QoS parameter as well as wavelength-continuity constraint. This paper proposes qualified path routing (QPR) algorithm with minimum computation and implementation complexity based on flooding method to accomplish QoS routing and wavelength assignment (RWA). We also introduce a QoS-based RWA mechanism considering multi-constraint such as optical signal quality attributes, survivability and wavelength-continuity constraint combined with proposed routing algorithm. Simulation results show superior efficiency of the proposed algorithms in terms of blocking probability, routing overhead and survivability ratio.

• Journal of the Korean Institute of Intelligent Systems
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• v.13 no.2
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• pp.169-174
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• 2003

The trial and success of malicious cyber attacks has been increased rapidly with spreading of Internet and the activation of a internet shopping mall and the supply of an online, or an offline internet, so it is expected to make a problem more and more. The goal of intrusion detection is to identify unauthorized use, misuse, and abuse of computer systems by both system insiders and external penetrators in real time. In fact, the general security system based on Internet couldn't cope with the attack properly, if ever. other regular systems have depended on common vaccine softwares to cope with the attack. But in this paper, we will use the positive selection and negative selection mechanism of T-cell, which is the biologically distributed autonomous system, to develop the self/nonself recognition algorithm and AIS (Artificial Immune System) that is easy to be concrete on the artificial system. For making it come true, we will apply AIS to the network environment, which is a computer security system.

• The KIPS Transactions:PartD
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• v.10D no.2
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• pp.301-308
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• 2003

In this paper, we propose a prototype system of digital data broadcasting system based on the ATSC data broadcasting standard. This prototype system uses data piping as a mechanism for delivery of arbitrary user-defined data inserted directly into the payload part of the MPEG-2 Transport Stream packets. This data type includes URL or HTML content. After the contents are inserted into the MPEG-2 Transport Stream, they can be delivered through the broadcasting to the DTV set-top receiver. The 75 packets received in real-time during the TV broadcast are used to start display or switch content. This prototype system describes how to achieve common design goals and integrating digital TV and web pages based on the ATSC data broadcasting standard. The prototype system can be used to display digital data contents - HTML, images-on existing TV or digital TV set-tops.

• Journal of the Institute of Electronics Engineers of Korea CI
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• v.46 no.4
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• pp.31-41
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• 2009

IEEE 802.11e proposed by IEEE 802.11 working group to guarantee QoS has contention based EDCA and contention free based HCCA. HCCA, a centralized polling based mechanism of 802.11e, needs a scheduling algorithm to allocate the network resource efficiently. The existing standard scheduler, however, is inefficient to support for QoS guarantee for real-time service having VBR traffic. To improve these limit, in this paper, we First, we propose priority polling algorithm which additionally considers the size of MSI and TXOP based on EDD algorithm to increase number of QSTAs. We also propose a dual token bucket TXOP allocation algorithm to reduce congestion caused by stations which enters network with considerable delay variance. TSPEC parameters, Maximum Burst Size (MBS) and Peak Data Rate (PR), are applied to design depth and token generation rate of two buckets. Simulation results show that the proposed algorithm has much higher performance compared with reference and SETT-EDD scheduler in terms of throughput and delay.

• The Bulletin of The Korean Astronomical Society
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• v.37 no.1
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• pp.89.2-89.2
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• 2012

Radiation Belt, discovered by Van Allen in 1958, is a region energetic particles are trapped by the Earth's magnetic field. To measure charged particles and fields in the radiation belt, RBSP(Radiation Belt Storm Probes) mission will be launched in September 2012 by NASA. RBSP mission consists of two spacecraft having orbit from 600 km to 30,000 km and rotates the Earth twice a day. This mission is not designed just for scientific purpose but have operational function broadcasting real time data for space weather monitoring. As a program of KASI-NASA cooperation, KASI is constructing RBSP data receiving antenna that will be installed by April in Daejeon. With this antenna system, NASA can receive RBSP data for 24 hours and KASI also get space weather information to protect Korean GEO satellites. In this presentation, we will discuss how we use RBSP data for space weather forecasting. In addition, we will talk about science topics that can be achieved by RBSP mission. Especially we focus on the dusk-side electron precipitation that has been considered as a main mechanism of electron dropout events. We show the dusk-side precipitation is closely associated with radiation belt electron loss with NOAA-POES data, and why RBSP mission is important to understand radiation belt physics.

• BMB Reports
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• v.40 no.6
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• pp.853-860
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• 2007

Resistance to anticancer drugs is a major obstacle in the effective treatment of tumors. To understand the mechanisms responsible for multidrug resistance (MDR), a proteomic approach was used to identify proteins that were expressed in different levels by the adriamycinresistant human gastric cancer cell line, SGC7901/ADR, and its parental cell line, SGC7901. Two-dimensional gel electrophoresis (2-DE) and image analysis was used to determine which protein spots were expressed in different levels by the two cell lines. These spots were then partially identified using ESI-Q-TOF mass spectrometry, and the differential expressional levels of the partially identified proteins were then determined by western blot analysis and real-time RT-PCR. Additionally, the association of Nucleophosmin (NPM1), a protein that was highly expressed by SGC7901/ADR, with MDR was analyzed using siRNA. As a result of this study, well-resolved, reproducible 2-DE patterns of SGC7901/ADR and SGC7901 were established, and 16 proteins that may playa role in the development of thermo resistance were identified. Additionally, suppression of NPMl expression was found to enhance adriamycin chemosensitivity in SGC7901/ADR. These results provide a fundamental basis for the elucidation of the molecular mechanism of MDR, which may assist in the treatment of gastric cancer.

• Biomolecules & Therapeutics
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• v.24 no.5
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• pp.495-500
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• 2016

This study aimed to explore the neuroprotection and mechanism of isoflurane on rats with spinal cord ischemic injury. Total 40 adult male Sprague-Dawley rats were divided into the four groups (n=10). Group A was sham-operation group; group B was ischemia group; group C was isoflurane preconditioning group; group D was isoflurane preconditioning followed by ischemia treatment group. Then the expressions of TWIK-related $K^+$ channel 1 (TREK1) in the four groups were detected by immunofluorescent assay, real time-polymerase chain reactions (RT-PCR) and western blot. The primary neurons of rats were isolated and cultured under normal and hypoxic conditions. Besides, the neurons under two conditions were transfected with green fluorescent protein (GFP)-TREK1 and lentivirual to overexpress and silence TREK1. Additionally, the neurons were treated with isoflurane or not. Then caspase-3 activity and cell cycle of neurons under normal and hypoxic conditions were detected. Furthermore, nicotinamide adenine dinucleotide hydrate (NADH) was detected using NAD+/NADH quantification colorimetric kit. Results showed that the mRNA and protein expressions of TREK1 increased significantly in group C and D. In neurons, when TREK1 silenced, isoflurane treatment improved the caspase-3 activity. In hypoxic condition, the caspase-3 activity and sub-G1 cell percentage significantly increased, however, when TREK1 overexpressed the caspase-3 activity and sub-G1 cell percentage decreased significantly. Furthermore, both isoflurane treatment and overexpression of TREK1 significantly decreased NADH. In conclusion, isoflurane-induced neuroprotection in spinal cord ischemic injury may be associated with the up-regulation of TREK1.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.9
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• pp.3917-3922
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• 2015

Curcumin, a lipid-soluble compound extracted from the plant Curcuma Longa, has been found to exert immunomodulatory effects via macrophages. However, most studies focus on the low bioavailability issue of curcumin by nano and microparticles, and thus the role of macrophages in the anticancer mechanism of curcumin has received little attention so far. We have previously shown the potential biocompatibility, biodegradability and anti-cancer effects of dendrosomal curcumin (DNC). In this study, twenty-seven BALB/c mice were equally divided into control as well as 40 and 80 mg/kg groups of DNC to investigate the involvement of macrophages in the antitumor effects of curcumin in a typical animal model of metastatic breast cancer. At the end of intervention, the tumor volume and weight were significantly reduced in DNC groups compared to control (P<0.05). Histopathological data showed the presence of macrophages in tumor and spleen tissues. Real-time PCR results showed that DNC increased the expression of STAT4 and IL-12 genes in tumor and spleen tissues in comparison with control (P<0.05), referring to the high levels of M1 macrophages. Furthermore treatment with DNC decreased STAT3, IL-10 and arginase I gene expression (P<0.05), indicating low levels of M2 macrophage. The results confirm the role of macrophages in the protective effects of dendrosomal curcumin against metastatic breast cancer in mice.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.11
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• pp.6315-6319
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• 2013

To study the differentiated expression of the proto-oncogene Pokemon in nasopharyngeal carcinoma (NPC) cell lines and tissues, mRNA and protein expression levels of CNE1, CNE2, CNE3 and C666-1 were detected separately by reverse transcription polymerase chain reaction (RT-PCR), real-time PCR and Western-blotting. The immortalized nasopharyngeal epithelial cell line NP69 was used as a control. The Pokemon protein expression level in biopsy specimens from chronic rhinitis patients and undifferentiated non keratinizing NPC patients was determined by Western-blotting and arranged from high to low: C666-1>CNE1>CNE2> CNE3>NP69. The Pokemon mRNA expression level was also arranged from high to low: CNE1>CNE2>NP69>C666-1>CNE3. Pokemon expression of NP69 and C666-1 obviously varied from mRNA to protein. The Pokemon protein level of NPC biopsy specimens was obviously higher than in chronic rhinitis. The data suggest that high Pokemon protein expression is closely associated with undifferentiated non-keratinizing NPC and may provide useful information for NPC molecular target therapy.