• Development and Reproduction
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• v.22 no.4
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• pp.309-318
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• 2018

The present study was conducted to investigate the regulatory mechanism of plasminogen activators (PAs) activation by $17{\beta}$-estradiol ($E_2$) and progesterone ($P_4$) in porcine uterine epithelial cells (pUECs). pUECs were collected from porcine uterine horn and cultured at 80% confluence. Then, 0.1% (v/v) DMSO, 20 ng/mL $E_2$, and $P_4$ with or without fetal bovine serum (FBS) treated to cultured cells for 24 hours. The supernatants were used for measurement of PAs activity and expression of urokinase-type PA (uPA), tissue-type PA (tPA), uPA specific receptor (uPAR), and type-1 PA inhibitor (PAI-1) mRNA were analyzed by real-time PCR. The expression of PAs-related genes was not affect by steroid hormones in both of serum treatment groups. However, PAs activity was increased by treatment of $E_2$ compared to 0.1% DMSO treatment in serum-free group (p<0.05). Then, $E_2$ and $P_4$ were diluted with 0.002% (v/v) DMSO for reduction of its effect and treated to cultured cells without FBS. Only tPA mRNA was significantly increased by $E_2$ treatment (p<0.05). PAs activity was enhanced in $E_2$ treated group compared to control groups (p<0.05). These results indicate that serum-free condition is more proper to evaluate effect of steroid hormones and activation of PAs in pUECs was mainly regulated by estrogen. These regulation of PAs activation may be associated with uterine remodeling during pre-ovulatory phase in pigs, however, further studies are needed to investigate precise regulatory mechanism.

• Journal of the Korean GEO-environmental Society
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• v.12 no.8
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• pp.5-12
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• 2011

This study was performed to analyze the degree of rainfalls, the duration time of rainfalls, the inclination of slopes and other damage causes depending on the ground condition, when the landslide occurs by the rainfall. Based on that, a big and a small model soil box were compared to examine the features of landslides, which is ultimately for forecasting landslides. An artificial rainfall device was installed in the model box, and the large model box has a real-size slope in it. Also, various measures were fulfilled such as collapse forms, a pore pressure, an earth pressure and moisture contents in order to analyze the effect of the model box size on the landslide mechanism and to test the usability of a small model box as a material for landslide experiments.

• Mycobiology
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• v.50 no.2
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• pp.121-131
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• 2022

The rare edible and medicinal fungus Antrodia cinnamomea has a substantial potential for development. In this study, Illumina HiSeq 2000 was used to sequence its transcriptome. The results were assembled de novo, and 66,589 unigenes with an N50 of 4413 bp were obtained. Compared with public databases, 6,061, 3,257, and 2,807 unigenes were annotated to the Non-Redundant, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes databases, respectively. The genes related to terpene biosynthesis in the mycelia of A. cinnamomea were analyzed, and acetyl CoA synthase (ACS2 and ACS4), hydroxymethylglutaryl CoA reductase (HMGR), farnesyl transferase (FTase), and squalene synthase (SQS) were found to be upregulated in XZJ (twig of C. camphora) and NZJ (twig of C. kanehirae). Moreover, ACS5 and 2,3-oxidized squalene cyclase (OCS) were highly expressed in NZJ, while heme IX farnesyl transferase (IX-FIT) and ACS3 were significantly expressed in XZJ. The differential expression of ACS1, ACS2, HMGR, IX-FIT, SQS, and OCS was confirmed by real-time quantitative reverse transcription PCR. This study provides a new concept for the additional exploration of the molecular regulatory mechanism of terpenoid biosynthesis and data for the biotechnology of terpenoid production.

• Journal of Veterinary Science
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• v.24 no.5
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• pp.69.1-69.11
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• 2023

Background: Kalkitoxin (KT) is an active lipopeptide isolated from the cyanobacterium Lyngbya majuscula found in the bed of the coral reef. Although KT suppresses cell division and inflammation, KT's mechanism of action in vascular smooth muscle cells (VSMCs) is unidentified. Therefore, our main aim was to investigate the impact of KT on vascular calcification for the treatment of cardiovascular disease. Objectives: Using diverse calcification media, we studied the effect of KT on VSMC calcification and the underlying mechanism of this effect. Methods: VSMC was isolated from the 6 weeks ICR mice. Then VSMCs were treated with different concentrations of KT to check the cell viability. Alizarin red and von Kossa staining were carried out to examine the calcium deposition on VSMC. Thoracic aorta of 6 weeks mice were taken and treated with different concentrations of KT, and H and E staining was performed. Real-time polymerase chain reaction and western blot were performed to examine KT's effect on VSMC mineralization. Calcium deposition on VSMC was examined with a calcium deposition quantification kit. Results: Calcium deposition, Alizarin red, and von Kossa staining revealed that KT reduced inorganic phosphate-induced calcification phenotypes. KT also reduced Ca⁺⁺-induced calcification by inhibiting genes that regulate osteoblast differentiation, such as runtrelated transcription factor 2 (RUNX-2), SMAD family member 4, osterix, collagen 1α, and osteopontin. Also, KT repressed Ca²⁺-induced bone morphogenetic protein 2, RUNX-2, collagen 1α, osteoprotegerin, and smooth muscle actin protein expression. Likewise, Alizarin red and von Kossa staining showed that KT markedly decreased the calcification of ex vivo ring formation in the mouse thoracic aorta. Conclusions: This experiment demonstrated that KT decreases vascular calcification and may be developed as a new therapeutic treatment for vascular calcification and arteriosclerosis.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.31 no.2B
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• pp.98-106
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• 2006

In order to provide a realtime transmission over a wireless LAM, we here present a new jamming based retransmission mechanism. In a legacy wireless LAN system, all stations use the binary exponential backoff algorithm to avoid collisions among frames. It is well known that the backoff algorithm causes more collisions as the numbers of active stations increases. This makes transmission of real time traffic hard. In the proposed scheme, when each station senses collisions, it promptly allows to send a jamming signal during a unique jamming window period which is determined by its own channel access count database(CACDB). This jamming windows is chosen not to be overlapped each other by using of CACDB, and thus channel access of another station is prevented. Hereafter the station gets the ownership of the medium when the wireless medium becomes idle after sending the jamming signal and sensing carrier, and then sends frame in medium. In our proposal, repeating collisions is never happened. We here assume that real time traffic use a frame of fixed length in order to make the time for receiving its ACK frame same. Comparing the proposed jamming-based retransmission scheme with the the 802.11 and 802.11e MAC by simulation. one can find that the proposed scheme have advantages in terms of delay, average backoff time, and average number of collisions per frame. One can find that the proposed scheme might be practically applicable to several applications of realtime traffic transmission in wireless LAN systems.

• BMB Reports
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• v.39 no.1
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• pp.9-15
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• 2006

To prove whether error catastrophe /lethal mutagenesis is the primary antiviral mechanism of action of ribavirin against foot-and-mouth disease virus (FMDV). Ribavirin passage experiments were performed and supernatants of $Rp_1$ to $Rp_5$ were harvested. Morphological alterations as well as the levels of viral RNAs, proteins, and infectious particles in the BHK-21 cells infected using the supernatants of $Rp_1$ to $Rp_5$ and control were measured by microscope, real-time RT-PCR, western-blotting and plaque assays, respectively. The mutation frequency was measured by sequencing the complete P1- and 3D-encoding region of FMDV after a single round of virus infection from ribavirin-treated or untreated FMDV-infected cells. Ribavirin treatment for FMDV caused dramatically inhibition of multiplication in cell cultures. The levels of viral RNAs, proteins, and infectious particles in the BHK-21 cells infected were more greatly reduced along with the passage from $Rp_1$ to $Rp_5$, moreover, nucleocapsid protein could not be detected and no recovery of infectious virus in the supernatant or detection of intracellular viral RNA was observed at the $Rp_5$-infected cells. A high mutation rate, giving rise to an 8-and 11-fold increase in mutagenesis and resulting in some amino acid substitutions, was found in viral RNA synthesized at a single round of virus infection in the presence of ribavirin of $1000\;{\mu}M$ and caused a 99.7% loss in viral infectivity in contrast with parallel untreated control virus. These results suggest that the antiviral molecular mechanism of ribavirin is based on the lethal mutagenesis/error catastrophe, that is, the ribavirin is not merely an antiviral reagent but also an effective mutagen.

• The Journal of Engineering Geology
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• v.14 no.1
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• pp.71-80
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• 2004

The failure of cut slopes frequently occurs particularly during the thawing season and the rain season in summer. This study interpreted data collected from site to which a real-monitoring system was applied in order to analyze the causes of ground behaviors and to forecast future slope failure. As for research methods, this study analyzed the size and mechanism of failure by integrating the results of field surveys and measurements. Furthermore, it analyzed data transmitted by the monitoring system installed in the a result, three times of ground displacement occurred as well as a number of partial tension cracks. The cut slope composed of sandstone and siltstone started its initial behavior as a result of torrential downpour and the loss of support of the substructure. For quantitative analysis of the characteristics of ground behavior, this study measured 5 lateral lines. According to the result of the measurement, displacement happened little in the section to which countermeasure had been applied, but displacement of maximum 400mm happened in the section to which countermeasure had not bee applied. The analysis of data on displacement and rainfall suggested a close relationship between ground behavior and rainfall. According to the result of stability interpretation along with the change of ground saturation, stability rate appeared to be less than 1.0 when ground saturation is over 55%. Although the current trend of ground behavior is at a stable stage falling within the range of tolerance, it is considered necessary to continue monitoring and data analysis because ground displacement is highly possible with the change of temperature during the winter.

• Proceedings of the Korea Inteligent Information System Society Conference
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• 2001.01a
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• pp.73-77
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• 2001

In this paper, we propose a virtual action system based on information agents, We call the system the MultiHammer, MultiHammer can be used for studying and analyzing online actions. MuiltiHammer provides functions of implement-ing a meta online action site and an experiment environ-ment. We have been using MultiHammer as an experiment as an experiment environment for BiddinBot. BiddingBot aims at assisting users to bid simultaneously in multiple online auctions. In order to bid simultaneously in multiple online auctions. In order to analyze the behavior of BiddngBot, we need to pur-chase a lot of items. It is hard for us to prepare a lot of fund to show usability and advantage of BiddingBot. MultiHam-mer enables us to effectively analyze the behavior of BiddingBot. MultiHammer consists of three types of agents for information collecting data storing and auctioning. Agents for information wrappers. To make agent work as wrarp-pers, we heed to realize software modules for each online action site. Implementing these modules reguires a lot of time and patience. To address this problem, we designed a support mechanism for developing the modules. Agents for data storing record the data gathered by agents for informa-tion collecting. Agents for auctioning provide online services using data recorded by agents for data storing. By recording the activities in auction sites. MultiHammer can recreate any situation and trace auction for experimentation, Users can participate in virtual using the same information in real online auctions. Users also participate in real auc-tions via wrapper agents for information collecting

• Journal of KIISE:Information Networking
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• v.30 no.2
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• pp.260-269
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• 2003

The traditional distributed object middleware platform is not suitable for the transmission of stream data, because RPC(Remote Procedure Call)-based message transmission have a great overhead. Therefore, the OMG(Object Management Group) proposes the AV(Audio and Video) stream reference model for streaming on the distributed object middleware platform. But, this reference model has not a detail of implementation. Particularly it also has not congestion control scheme for improvement of network efficiency on the real network environment. It is a very important and difficult technical issue to provide the stream transmission platform with advanced congestion control scheme. In this paper, we propose an architecture of a distributed stream transport platform and deal with the design and implementation concept of our proposed platform. Also, we present a mechanism to improve streaming utilization by SRTP(Smart RTP). SRTP is our proposed TCP-Friendly scheme.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.25 no.5
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• pp.352-360
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• 2015

Squeal noise is a harsh, high-pitched sound that occurs when railways are running at sharp curve tracks. The cause of squeal noise is known to be the transient lateral traction force between wheel and rail. Field measurements are too difficult to control the parameters. Thus, the scaled test rig should have been made in order to investigate the generating mechanism of squeal noise. The unique feature of our test rig, HSTR(Hongik Squeal Testing Rig), is that DOFs of its wheelset are as close to as those of the real railway. The attack angle and running speed of the rail roller are controlled in real time for simulating a transient characteristic of driving curve. The environment conditions, such as given axle load, running speed, and wheel's yaw angle have been identified for generating squeal noise and the squeal noise itself has been measured. The relation between wheel creepage and creep force in lateral direction and the criteria for squeal noise have been investigated, which results has been verified by finite element method.