• 에너지공학
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• 제8권4호
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• pp.605-611
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• 1999

화력발전설비 고온요소의 잔여수명평가는 주로 비파괴검사 및 검사결과의 파괴역학적 해석등으로 행해졌다. 그러나 기존 방법은가동중에는 적용되기 어렵고 급작스런 운전이력에 대한 정량적인 손상을 평가할 수 없었다. 따라서 구조물의 형상, 운전이력 및 재료물성에 근거하여 실시간 수명소비율 및 잔여수명을 평가하는 기술의 개발이 요구되고 있다. 본 연구에서는 고온 증기헤더의 수명을 실시간으로 평가하는 감시시스템 소프트웨어를 개발하였다. 고온헤더에 대하여 해석된 온도, 응력, 그린함수를 이용하여 취약부위의 실시간 응력을 계산하고 천이 사이클을 카운트하여 크리프 및, 피로수명을 계산하였다. 본 소프트웨어를 개발함으로써 운전이력에 따른 소비수명을 실시간으로 평가하고 운전정보를 기록하여 향후 운전계획, 보수주기 및 교체시기를 결정하는데 지침이 되도록 하였다.

• Journal of Microbiology and Biotechnology
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• 제22권9호
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• pp.1301-1306
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• 2012

We combined real-time RT-PCR and real-time PCR (R/P) assays using a hydrolysis probe to detect Mycobacterium tuberculosis complex (MTBC)-specific 16S rRNA and its rRNA gene (rDNA). The assay was applied to 28 non-respiratory and 207 respiratory specimens from 218 patients. Total nucleic acids (including RNA and DNA) were extracted from samples, and results were considered positive if the repeat RT-PCR threshold cycle was ${\leq}35$ and the ratio of real-time RT-PCR and real-time PCR load was ${\geq}1.51$. The results were compared with those from existing methods, including smear, culture, and real-time PCR. Following resolution of the discrepant results between R/P assay and culture, the overall sensitivity, specificity, positive predictive values (PPV), and negative predictive values (NPV) of all samples (including non-respiratory and respiratory specimens) were 98.2%, 97.2%, 91.7%, and 99.4%, respectively, for R/P assay, and 83.9%, 89.9%, 72.3%, and 94.7%, respectively, for real-time PCR. Furthermore, the R/P assay of four patient samples showed a higher ratio before treatment than after several days of treatment. We conclude that the R/P assay is a rapid and accurate method for direct detection of MTBC, which can distinguish viable and nonviable MTBC, and thus may guide patient therapy and public health decisions.

• 한국어병학회지
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• 제34권2호
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• pp.161-168
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• 2021

Quantitative analysis of myxosporean parasites (Enteromyxum leei and Parvicapsula anisocaudata) were performed using real-time PCR on the internal organs (head kidney, body kidney, intestine, spleen, brain, liver, heart, muscle, blood, and eye) of emaciated Paralichthys olivaceus from farm-A. The highest DNA copy number of E. leei was shown in the intestine (1.3 × 10⁸ copies/mg tissue) of emaciatied P. olivaceus and DNA copy number in the other internal organs (1.3 × 10³~4.6 × 10⁵ copies/mg tissue) showed lower than in intestine. From the result of real-time PCR for P. anisocaudata, it was considered mildly infected, due to the low DNA copy numbers of the head kidney (1.3 × 10³ copies/mg tissue) and body kidney (9.1 × 10³ copies/mg tissue). In order to investigate whether myxosporean parasites can be detected in a non-invasive way, quantitative analysis of E. leei and P. anisocaudata from rearing water of three farms were performed by real-time PCR. The DNA copy number of E. leei from rearing water of farm-A and farm-B were 8 × 10⁴ and 5 × 10⁵ copies/L, respectively. However, it was not detected in farm-C. For P. anisocaudata from rearing water, farm-A, farm-B and farm-C showed 0, 2.0 × 10⁶ and 5.1 × 10⁶ copies/L, respectively.

• 한국멀티미디어학회논문지
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• 제10권12호
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• pp.1714-1725
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• 2007

This paper describes a top-down behavior planning framework in a simulation game from personality to real life action selection. The combined behavior creating system is formed by five levels of specification, which are personality definition, motivation extraction, emotion generation, decision making and action execution. Along with the data flowing process in our designed framework, NPC selects actions autonomously to adapt to the dynamic environment information resulting from active agents and human players. Furthermore, we illuminate applying Gaussian probabilistic distribution to realize character's behavior changeability like human performance. To elucidate the mechanism of the framework, we situated it in a restaurant simulation game.

• 대한수학교육학회지:학교수학
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• 제3권2호
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• pp.233-248
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• 2001

In pthis aper, mathematical terms in 7th elementary mathematics curriculum(from now, in short, 7th curriculum)are reexamined critically. In 7th curriculum there are 123 terms, which seems to be selected cautiously But it is not sure. There are lots of evidences for selecting terms incautiously, Through these evidences, following conclusions are induced: (1) Terms were not selected strictly. There are many terms omitted in 7th curriculum, which are necessary for understanding mathematical concepts. (2) There were no rational principles for selecting terms in 7th curriculum. Any rational principles can not be found out among terms in 7th curriculum. (3) Mathematical terms and real life terms in 7th curriculum were not distinguished explicitly. There were some real life terms in 7th curriculum, which were significant for understanding mathematical concepts. But other real life terms which is significant also for understanding mathematical concepts were not contained in 7th curriculum.

• Journal of Information Technology Applications and Management
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• 제21권3호
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• pp.97-117
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• 2014

People can differently act online than in real-life. Their virtual identities may allow them to express themselves more freely. In this perspective, there is a self-discrepancy between virtual- and real-life. Due to the anonymity, people can feel free to express who actually they are online without considering social pressure. Especially, for those people who live in a repressed society, virtual-selves can play a significant role to achieve their well-being. For this reason, a self-discrepancy between actual- and virtual-self related to well-being was studied. With 183 survey responses, political freedom, extraversion and neuroticism are identified as significant influencers of subjective well-being (SWB). In addition, an increase in self-discrepancy between virtual- and real-life for 'unhappy' people leads to an increased online well-being, which is contradictory to Higgins' self-discrepancy theory.

• Asia Marketing Journal
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• 제21권4호
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• pp.45-57
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• 2020

With the development of new technologies, virtual reality (VR) is emerging as a new consumer marketing platform. Yet, only a few studies have investigated VR in the consumption context. The current research explores how experiencing a product through VR influences consumer attitude and purchase intention for the product. Although assessing products in real life is believed to be more diagnostic than that through VR, the present research revealed that experiencing a product through VR could lead to a more positive attitude toward and higher purchase intention for the product. We also demonstrated that "enjoyment" has a mediating effect while evaluating the product through VR. To compare product experience through VR to that in real life, we employed the HTC Vive VR system to implement a VR environment. In the VR condition, participants evaluated plastic building blocks using a VR headset and a motion controller; and in the real life (reality) condition, they evaluted plastic building blocks printed using a 3D printer to make them look exactly the same as the blocks used in the VR condition. Finally, implications and suggestions are discussed.

• Asia Marketing Journal
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• 제25권2호
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• pp.61-70
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• 2023

Although research on Virtual Reality (VR) has uncovered numerous technical advantages of VR over traditional media, little is known about how individual VR users with varying prior experience respond to VR differently. This paper examines the effect of users' prior VR experience on their subsequent real-life behavior in the domain of charitable consumption. Specifically, we find that compared to experienced VR users, novice VR users are more likely to support a charitable cause in real life (e.g., ocean conservation) after experiencing this cause in VR. The increased support among novice VR users occurs because they perceive the use of VR to be more novel. We find a boundary of this effect such that when VR is used to promote a noncharitable cause, novice VR users no longer increase their real-life support after VR. This research offers new possibilities for future studies on the use of VR in societal marketing.

• 미생물학회지
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• 제43권2호
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• pp.91-99
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• 2007

인간면역결핍바이러스(Human immunodeficiency virus; HIV) 진단을 위한 다중, 초고속실시간 PCR법을 개발하였다. 검출대상의 DNA 염기서열은 env 유전자를 기반으로 설계되었으며, 각기 HIV-1 특이 495염기(gi_1184090) 및 HIV-2 특이 294염기(gi_1332355)의 DNA를 안정상의 이유로 PCR을 이용한 유전자합성법으로 제작하여 사용하였다. 초고속 실시간 PCR은, PCR의 회전 중 각 단계별 설정시간을 극단적으로 축소하여, $1\;{\mu}l$의 PCR 용액용 microchip을 탑재할 수 있는 $Genspector^{TM}$을 사용하여 수행하였다. DNA 증폭과 융점분석을 포함한 총 PCR 검색 시간은 HIV_1 및 HIV-2 모두에서 15분 이내로 완료되었으며, 각기 최소 2.3개의 합성 env 유전자로부터도 HIV-1 특이 117염기와 HIV-2 특이 119염기의 PCR산물을 성공적으로 증폭시킬 수 있는 민감성을 보여주었다. 이런 형식의 실시간 PCR법을 본 연구에서 초고속실시간 PCR (Ultra-rapid real-time PCR)이라 명명하였다. 이는 본 연구의 대상인 HIV에 대한 보조적 진단방법일 뿐 아니라 PCR 검색법이 사용되고 있는 다른 병원체에 대하여도 적용될 수 있을 것이나, 우선 HIV 임상시료에 대한 본 검색법의 효용성 실험 등 추가 연구가 필요할 것으로 사료된다.

• 센서학회지
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• 제22권4호
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• pp.262-267
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• 2013

We developed a real-time screening system using optical coherence tomography (OCT) to distinguish the fruitful melon seeds efficiently. Cross-section images of melon seeds infected with Cucumber green mottle mosaic virus (CGMMV) showed an additional layer that did not appear in normal seeds. Additional layer appeared under $100{\sim}300{\mu}m$ from the surface of the seed. OCT can visualize the micro-structural and morphological changes of the internal seed structure. Real-time OCT seed screening system provided the real-time, non-destructive, cross-section image and quantitative information such as A-scan analysis of selected region in the cross-section image. We can distinguish the viral infection seeds while monitoring the averaged A-scan analysis graph in real-time by considering the second peak value of the graph which refers to the layer that occurred owing to the virus. Real-time OCT seed screening system could assist to distinguish the disease caused by CGMMV.