• 농촌지도와개발
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• 제16권4호
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• pp.1021-1048
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• 2009

Based on Rural living indicator -2005 survey data, in RDA this study attempted to investigate Rural Residents' real life satisfaction or identity model by the types of leisure consumption depending on each generation and explored the relationship between leisure consumption and the enhancement of self-identity/ interpersonal relationship, and that between leisure consumption satisfaction and real life satisfaction. Based on systematic random sampling with constructed questionnaires, 1,870 data were collected and analyzed. Overall, the results of this study showed that there are statistically significant differences between generation in leisure consumption. In detail, the findings of this study are as follows. The types of Rural Residents' leisure consumption can be divided into three styles including Semi-leisure type, Passive leisure type and Total leisure type consumption. The preferred types of leisure consumption of Rural Residents' were Semi-leisure type, Passive leisure type and Total leisure type in order. Except for times-pending leisure culture consumption, Semi-leisure type and Passive leisure type influenced on the enhancement of self-identity and interpersonal relationship, then the enhancement of self-identity and interpersonal relationship influenced on leisure consumption-satisfaction, and finally leisure consumption satisfaction influenced on real life satisfaction.

• 한국가정과교육학회지
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• 제6권2호
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• pp.59-71
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• 1994

The purpose of this study was to analyze the problems of housing education in Home Economics and then to present some suggestion for improving the housing education in the middle school and to analyze the problems of housing edcation that was investigated on the recognition and application in real life of housing area subject to male/female students in the middle school. The respondents were 600 boys and girls of middle school. Statistical methods adopted for data analysis were Frequency, Chi-square, t-test, F-test. The major findings were follows: 1. Many students(67.2%) responded that housing of home economics was wholly helpful in the actual life. 2. Many students recognized the necessity for the housing education. They particularly recognized the need about the knowlidge of sanitation and facilities of housing. 3. The degree to which housing education applied in the real life was lower than that to which it was needed. 4. There was a tendency that the more students realized the usefulness of housing education, the more positively they applied it in the real life. 5. The contents that students preferred to be additionally included in housing education were improvement of residential environment, solutions to housing environmental deterioration and pollution, future housing, and housing for the elderly.

• International Journal of Fluid Machinery and Systems
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• 제10권1호
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• pp.40-46
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• 2017

Small valves including ball valves, gate valves and butterfly valves have been adopted in the fields of steam power generation, petrochemical industry, carriers, and oil tankers. Butterfly valves have normally been applied to fields where in narrow places installing the existing valves such as gate valves and ball valves have proven difficult due to the surrounding area and the heavier of these valves. Butterfly valves are used to control the mass flow of the piping system under low pressure by rotating the circular disk installed inside. The butterfly valve is benefitted by having simpler structure in which the flow is controlled by rotating the disc circular plate along the center axis, whereas the weight of the valve is light compared to the gate valve and ball valve above-mentioned, as there is no additional bracket supporting the valve body. The manufacturing company needs to acquire the performance and life test equipment, in the case of adopting the improving factors to detect leakage and damage on the seat of the valve disc. However, small companies, which are manufacturing the industrial valves, normally sell their products without the life test, which is the reliability test and environment test, because of financial and manpower problems. Furthermore, the failure mode analysis of the products failed in the field is likewise problematic as there is no system collecting the failure data on sites for analyzing the failures of valves. The analyzing and researching process is not arranged systematically because of the financial problem. Therefore this study firstly tried to obtain information about the failure data from the sites, analyzed the failure mode based on the field data collected from the customers, and then obtained field data using measuring equipment. Secondly, we designed and manufactured the performance and life test equipment which also have the real time monitoring system with the naked eye for the butterfly valves. The concept of this equipment can also be adopted by other valves, such as the ball valve, gate valve, and various others. It can be applied to variously sized valves, ranging from 25 mm to large sized valves exceeding 3000 mm. Finally, this study carries out the life test with square wave pressure, using performance and life test equipment. The performance found out that the failures from the real time monitoring system were good. The results of this study can be expanded to the other valves like ball valves, gate valves, and control valves to find out the failure mode using the real time monitoring system for durability and performance tests.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2007년도 제48회 학술심포지움 및 추계국제학술대회
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• pp.107.2-107.2
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• 2007

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• Bulletin of the Korean Chemical Society
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• 제29권5호
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• pp.943-947
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• 2008

A phosphate-specific and fluorescent probe was prepared for label-free phosphatase assays based on fluorescence polarization. By using the probe, dephosphorylation reactions of DNA and protein substrates by calf intestinal alkaline phosphatase (CIP) could effectively be monitored in real-time. Since this assay method does not require additional materials such as labeled substrates and phosphospecific antibodies to obtain fluorescence polarization signals, it is simple, cost-effective, and expected to be useful not only for measuring activity of phosphatases but also for high-throughput screening of phosphatase inhibitors.

• International Journal of Industrial Entomology and Biomaterials
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• 제12권1호
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• pp.15-19
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• 2006

RNA interference has been used as a powerful tool in preventing virus proliferation in many species. In this study, we injected the dsRNA in vitro transcripts into Bombyx mori to investigate the resistance to B. mori nuclear polyhedrosis virus (BmNPV). Through vivisectional observation and real-time quantities PCR analysis, we found that these dsRNA can prevent the BmNPV to a certain extent, and delay the viruses' proliferation.

• 국제학술발표논문집
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• The 6th International Conference on Construction Engineering and Project Management
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• pp.376-380
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• 2015

Life-Cycle Cost Analysis (LCCA) for highway projects is known as an effective analytical technique that uses economic principles to evaluate long-term alternative investment options, especially for comparing the values of alternative pavement design structures and construction strategies. In the Unites States, the 2012 Moving Ahead for Progress in the 21^st Century Act (MAP-21) amended the United States Code to mandate that the United States Government Accountability Office (GOA) conducts a study of the best practices for calculating life-cycle costs and benefits for the federally funded highway projects in 2013. The RealCost 2.5CA program was developed and adapted as an official LCCA tool to comply with regulatory requirements for California state highway projects in 2013. Utilization of this California-customized LCCA software helps Caltrans to achieve substantial economic benefits (agency cost and road user cost savings) for highway projects. Proper implementation of LCCA for roadway construction and rehabilitation would deliver noticeable savings of agency's roadway maintenance cost especially in developing counties where financial difficulties exist.

• 수완나부미
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• 제1권1호
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• pp.27-50
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• 2009

Although Malaysia chooses Islam as national religion, the nation has really shown a variety of belief systems among which we would find Hinduism, Buddhism, and Animism. Most scholars having studied them regarded the aspect of Malay traditional belief systems as syncretism. This trait of the belief systems in Malaysia is revealed well in the sphere specifically as the rite of passage, to which I do rather prefer the rite of "life" from the sense of emphasizing the rituals varied by the cycle of life time. Under the perspective this paper examines the real phases of the rite of passage, or life of Malays. I will argue here the fact that in performing the rite of passage, Malays mobilize various arts on the basis of the syncretic faith accepting the supernatural, or keramat, with almighty power. Therefore, from the study of the rite of passage we can exactly scrutinize how Malays have altered and adjusted their belief systems in real world.

• 한국해양학회지:바다
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• 제22권1호
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• pp.31-44
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• 2017

Pfiesteria piscicida는 유독 종속영양 와편모조류로서, 크기가 작고 형태학적으로 유사한 Pfiesteria-like dinoflagellate (PLD) 종들로 인해, 광학 현미경 관찰만으로 정확하게 동정하는 것이 불가능하다. 따라서, 본 연구에서는 이러한 한계점을 극복하기 위해 EvaGreen 기반의 정량적 real-time PCR기법을 개발하였으며, 한국 근해에서 P. piscicida의 분포와 시화호에서 개체군 변동 조사를 통해 현장에서 유용성을 검증하였다. 이를 위해, internal transcribed spacer 1 (ITS 1) 영역을 대상으로 종 특이적 프라이머를 제작하였으며, P. piscicida와 진화적 유연관계에 있는 다양한 미세조류에 대해 PCR을 수행하여 프라이머의 특이성을 검증하였다. 개발된 프라이머를 real-time PCR 기법에 적용한 결과, P. piscicida의 세포수와 $C_T$값 간의 유의한 표준 곡선($r^2{\geq}0.999$)과 하나의 융해곡선 피크($88^{\circ}C$)가 관찰되었다. 이는 본 연구에서 개발된 기법이 대상생물인 P. piscicida를 정확하게 정성 및 정량분석이 가능함을 의미한다. 개발된 real-time PCR 기법의 현장적용 결과, 광학 현미경상에서는 탐지할 수 없었던 P. piscicida를 서해(김제, 목포)와 동해(강릉) 시료에서 검출하였다. 또한, 시화호 시료를 이용한 P. piscicida개체군 동태 조사에서 다른 정점에 비해 염분도가 상대적으로 낮았던(${\leq}15psu$), St. 1에서 2007년 6, 7, 8월에 세포밀도의 피크가 관찰되었다. 본 연구에서 개발된 EvaGreen 기반 real-time PCR 기법은 현장에서 P. piscicida를 탐지 및 정량 분석 하는데 성공하였으며, 이는 향후 이들 종에 대한 다양한 생태학적 연구에 활용될 것으로 사료된다.

• 미생물학회지
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• 제43권1호
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• pp.23-30
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• 2007

조류 인플루엔자바이러스(AIV) H5N1 아형을 Real-time PCR법을 이용하여 가장 빠르게 진단할 수 있는 방법을 개발하였다. 검색 대상의 염기서열은 AIV H5N1 아형의 hemagglutinin 유전자 중 가장 상동성이 높은 387 bp의 부위를 선택하였고, 실험의 안전을 위하여 인공합섬의 방법으로 제작하였다. Microchip을 기반으로 한Real-time PCR법을 사용하였으며, 총PCR 반응액의 양을 $1{\mu}l$로, PCR 과정 중 각 단계, 즉 해리, 접합, 신장의 시간을 각1초, 1초, 3초로 하여 총 실험시간을 단축하였다. 진단을 위한 실험과정에서 PCR 및 융점분식에 소요된 최단 시간은 12분28초였으며, 민감도측정에서 최소2.4개의 hemaggutinin 유전자를 기질로 하여 목적한 특이 189 bp의 PCR 산물을 증폭할 수 있었기에, 본 연구에서는 이런 초고속 PCR 실험방식을 Quick Real-time PCR이라 명명하였다. 이 결과들은 가금류 및 사람에게 전파된 AIV H5N1아형의 진단에 적용될 수 있을 뿐 아니라, PCR이 사용되는 다른 신속검색법에도 널리 적용 될 수 있을 것으로 기대한다.