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RNA Interference to Prevent Bombyx mori Nuclear Polyhedrosis Virus Infection in Vivo  

Hu Zhigang (Institute of Life Sciences, JiangSu University)
Chen Keping (Institute of Life Sciences, JiangSu University)
Gao Lu (Institute of Life Sciences, JiangSu University)
Yao Qin (Institute of Life Sciences, JiangSu University)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.12, no.1, 2006 , pp. 15-19 More about this Journal
Abstract
RNA interference has been used as a powerful tool in preventing virus proliferation in many species. In this study, we injected the dsRNA in vitro transcripts into Bombyx mori to investigate the resistance to B. mori nuclear polyhedrosis virus (BmNPV). Through vivisectional observation and real-time quantities PCR analysis, we found that these dsRNA can prevent the BmNPV to a certain extent, and delay the viruses' proliferation.
Keywords
Bombyx mori nuclear polyhedrosis vinus; DsRNA; RNAi; Real-time quantities PCR; Silkworm;
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