• Journal of The Korean Association For Science Education
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• v.5 no.2
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• pp.81-88
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• 1985

The purpose of the study was to establish a new view of textbook which may contribute to the abolishment of the instruction mainly based on the only textbook and to the promotion of creativities of students. We first reviewed science textbooks and related teaching-learning materials of foreign countries with emphasis on the relationship among textbook and teaching-learning materials and practical use of them. In western countries the roles of traditional textbook has been changed. That is, various kinds of materials such as reading book, work-book, worksheet, experimental guidebook, filmstrips are used to raise effect of instruction besides of traditional type of textbook. Secondary, we identified the problems related to the science textbook-view of textbook, textbook contents, practical use of textbook-through opinion survey administered to principals akd teachers of elementary schools. The results of the survey are as follows; Concerning the view of textbook, most teachers did not recognize textbook as an absolute materials. They thought that textbook contents could be taught reorganized according to their judgements. On the contrary, teachers responded to the question of whether or not they follow contents of textbook as they are presented in it were approximately 30%. Further, more than 75% of them have seldom used instructional materials except textbooks. In order to revise the problems of our present textbook as stated above, a new view of textbook should be established. We, above all, established 4 basic premises for searching a new view of textbook. 1) Textbook should not be considered as the only material but as being at the center of various teaching -learning materials. 2) The importance of textbook should be illustrated Among Curriculum, textbook and related teaching-learning materials, instruction and evaluation. 3. Textbook contents should not be regarded as definitely fixed or absolute ones. 4. Human being can understand environment more fully by commanding his swnsory organ multilaterally. Under these premises we disscussed about curriculum and textbook, textbook, and instruction, akd evaluation method.

• BMB Reports
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• v.40 no.3
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• pp.358-367
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• 2007

A novel mannose-binding tuber lectin with in vitro antiproliferative activity towards human cancer cell lines and antiviral activity against HSV-II was isolated from fresh tubers of a traditional Chinese medicinal herb, Typhonium divaricatum (L.) Decne by a combined procedure involving extraction, ammonium sulfate precipitation, ion exchange chromatography on DEAE-SEPHAROSE, CM-SEPHAROSE and gel-filtration on sephacryl S-200. The apparent molecular mass of the purified Typhonium divaricatum lectin (TDL) was 48 kDa. TDL exhibits hemagglutinating activity toward rabbit erythrocytes at 0.95 $\mu$g/ml, and its activity could be strongly inhibited by mannan, ovomucoid, asialofetuin and thyroglobulin. TDL showed antiproliferative activity towards some well established human cancer cell lines, e.g. Pro-01 (56.7 $\pm$ 6.8), Bre-04 (41.5 $\pm$ 4.8), and Lu-04 (11.4 $\pm$ 0.3). The anti-HSV-II activity of TDL was elucidated by testing its HSV-II infection inhibitory activity in Vero cells with $TC_50$ and $EC_50$ of 5.176 mg/ml and 3.054 $\mu$g/ml respectively. The full-length cDNA sequence of TDL was 1145 bp and contained an 813-bp open reading frame (ORF) encoding a 271 amino acid precursor of 29-kDa. Homology analysis showed that TDL had high homology with many other mannose-binding lectins. Secondary and three-dimensional structures analyses showed that TDL is heterotetramer and similar with lectins from mannose-binding lectin superfamily, especially those from family Araceae.

• The Plant Pathology Journal
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• v.33 no.3
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• pp.318-328
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• 2017

Chitinase-producing Paenibacillus elgii strain HOA73 has been used to control plant diseases. However, the antimicrobial activity of its extracellular chitinase has not been fully elucidated. The major extracellular chitinase gene (PeChi68) from strain HOA73 was cloned and expressed in Escherichia coli in this study. This gene had an open reading frame of 2,028 bp, encoding a protein of 675 amino acid residues containing a secretion signal peptide, a chitin-binding domain, two fibronectin type III domains, and a catalytic hydrolase domain. The chitinase (PeChi68) purified from recombinant E. coli exhibited a molecular mass of approximately 68 kDa on SDS-PAGE. Biochemical analysis indicated that optimum temperature for the actitvity of purified chitinase was $50^{\circ}C$. However, it was inactivated with time when it was incubated at $40^{\circ}C$ and $50^{\circ}C$. Its optimum activity was found at pH 7, although its activity was stable when incubated between pH 3 and pH 11. Heavy metals inhibited this chitinase. This purified chitinase completely inhibited spore germination of two Cladosporium isolates and partially inhibited germination of Botrytis cinerea spores. However, it had no effect on the spores of a Colletotricum isolate. These results indicate that the extracellular chitinase produced by P. elgii HOA73 might have function in limiting spore germination of certain fungal pathogens.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.11
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• pp.1576-1584
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• 2016

Acetyl xylan esterase (AXE), which hydrolyzes the ester linkages of the naturally acetylated xylan and thus known to have an important role for hemicellulose degradation, was isolated from the anaerobic rumen fungus Neocallimastix frontatlis PMA02, heterologously expressed in Escherichi coli (E.coli) and characterized. The full-length cDNA encoding NfAXE1 was 1,494 bp, of which 978 bp constituted an open reading frame. The estimated molecular weight of NfAXE1 was 36.5 kDa with 326 amino acid residues, and the calculated isoelectric point was 4.54. The secondary protein structure was predicted to consist of nine ${\alpha}$-helixes and 12 ${\beta}$-strands. The enzyme expressed in E.coli had the highest activity at $40^{\circ}C$ and pH 8. The purified recombinant NfAXE1 had a specific activity of 100.1 U/mg when p-nitrophenyl acetate (p-NA) was used as a substrate at $40^{\circ}C$, optimum temperature. The amount of liberated acetic acids were the highest and the lowest when p-NA and acetylated birchwood xylan were used as substrates, respectively. The amount of xylose released from acetylated birchwod xylan was increased by 1.4 fold when NfAXE1 was mixed with xylanase in a reaction cocktail, implying a synergistic effect of NfAXE1 with xylanase on hemicellulose degradation.

• The Korean Journal of Community Living Science
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• v.20 no.2
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• pp.145-156
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• 2009

The purpose of this study was to explore socio-economic factors as determinants of food behavior and self-evaluation on meeting dietary guidelines. The data were derived from the KNHANES collected in 2007. A multidimensional framework of the determinants of food behavior was used, including age, gender, region, occupation, education, income and nutritional knowledge. The determinants of food behavior and self-evaluation were estimated by ordered logistic regression models. Food behavior was measured by dietary diversity scores including six food groups, which were cereals, vegetables, meats, fruits, milk, and oils. Self-evaluation on meeting dietary guidelines was based on responses from questionnaires for implementing Korean dietary guidelines. In general, the respondents who fulfilled all criteria were few. There were some differences between dietary diversity scores and self-evaluation on meeting dietary guidelines. Age, gender, and educational level showed effect on food behavior and self-evaluation. For dietary diversity scores, the individuals who were younger male, graduated from college were more likely to consume more various foods. The individuals who were older female, graduated from high school were more likely to meet dietary guidelines. Occupation was associated only with self-evaluation. Age and gender were associated with food behavior as well as self-evaluation. Income and marital status were associated only with dietary diversity scores. Reading food label and occupation were associated only with self-evaluation. The food behavior of married individuals was less in line with the dietary diversity scores than singles. In conclusion the differences between objective measure and subjective measure on individuals' diet showed more efforts like segmented nutritional education would be needed to increase the quality of dietary life.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.12
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• pp.1684-1690
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• 2014

Estrogen and its receptors are essential hormones for normal reproductive function in males and females during developmental stage. To better understand the effect of estrogen receptor (ER) gene in yak (Bos grunniens), reverse transcription-polymerase chain reaction (PCR) was carried out to clone $ER{\alpha}$ and $ER{\beta}$ genes. Bioinformatics methods were used to analyze the evolutionary relationship between yaks and other species, and real-time PCR was performed to identify the mRNA expression of $ER{\alpha}$ and $ER{\beta}$. Sequence analysis showed that the ER open reading frames (ORFs) encoded 596 and 527 amino acid proteins. The yak $ER{\alpha}$ and $ER{\beta}$ shared 45.3% to 99.5% and 53.9% to 99.1% protein sequence identities with other species homologs, respectively. Real-time PCR analysis revealed that $ER{\alpha}$ and $ER{\beta}$ were expressed in a variety of tissues, but the expression level of $ER{\alpha}$ was higher than that of $ER{\beta}$ in all tissues, except testis. The mRNA expression of $ER{\alpha}$ was highest in the mammary gland, followed by uterus, oviduct, and ovary, and lowest in the liver, kidney, lung, testis, spleen, and heart. The $ER{\beta}$ mRNA level was highest in the ovary; intermediary in the uterus and oviduct; and lowest in the heart, liver, spleen, lung, kidney, mammary gland, and testis. The identification and tissue distribution of ER genes in yaks provides a foundation for the further study on their biological functions.

• KIEAE Journal
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• v.2 no.4
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• pp.41-47
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• 2002

East Asian explain the changes of substance happened on earth and those of human life with the conceptual frame of "Gi-ki"(地氣; earth vital energy) and organize them by the system of "Feng-shui(風水) theory. The core of Feng-shui theory is reading the expression of the nature and feeling the Gi-ki from the expression. One of the properties of the earth is that the earth has a magnetic field associated with it- the Geomagnetic field. The geomagnetic field is produced by a combination of the effects of electric currents in the earth's liquid core, the magnetization of crustal rocks, external electric current systems that surround the earth and currents induced in the outer layers of the earth by magnetic field variations. The sameness of logic between Feng-shui and geopathic zones is that both are concerned with the discrimination of site and the energy of places, in other words both disciplines are concerned with how the environment can influence people. In this context the operation of Gj-ki can be related with the effect caused by geomagnetic field on site. In this study Jongmyo(宗廟), one of the representative traditional architecture in Korea is selected because the site selection and building layout follows Feng-shui faithfully according to Taejo silok (太祖實錄, Annals of King Taejo). Observing the landforms surrounding Jongmyo, Jongmyo is apparently located in auspicious places named Gumge Poranhyoeng(金鷄抱卵形, a Feng-shui landscape of golden hen sitting on eggs). The geomagnetic investigation of Jongmyo shows that the geomagnetic values of Toekan(the space near Hyeol) are relatively high and uniform and those of Sangweoldae and Haweoldae are decreased in accordance with the distance. The result shows that there is possibility that Feng-shui has scientific basis related with geomagnetic field. Feng-shui theory can suggest a direction for designing the sustainable building for living with nature.

• The Journal of the Korea Contents Association
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• v.11 no.11
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• pp.286-295
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• 2011

This case study was to examine the effect of the sensory integration therapy on the Goldenhar syndrome child with tactile defensiveness, developmental delay and occupational performance difficulties. The child was 7.10 year old boy. For intervention, four evaluations were used and the results are as follows. First, in Canadian Occupational Performance Measure showed occupational performance goal as reading and writing in upright position. Second, in Short sensory profile and sensory history, significant differences between sensory sensitivity and tactile processing. Third, in Developmental Profile-3 showed delay. Fourth, in Bruininks-Oseretsky Test of Motor Proficiency-2 showed below average. After intervention, the results of which showed a positive changes. Therefore, this case study is found that the sensory integration therapy is effective in the Goldenhar syndrome child with tactile defensiveness and developmental delay.

• Journal of Korean Medicine Rehabilitation
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• v.21 no.2
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• pp.203-210
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• 2011

Objectives : The aim of this study is to establish evidence-based guideline on infrared therapy on acupoints. Methods : We investigated all the articles in websites including Pubmed, China national knowledge Infrastructure(CNKI) and medical journals authorized by national research foundation of Korea. The key words are acupoints and infrared. For the first time, we withdrew some papers no relationship to key words by reading titles and abstracts. In the second, we only selected randomized clinical trials(RCT) published from 2000 to 2010. Results : Out of 62 papers, only 2 RCT were analyzed. One (RCT 1) was about depression patients with insomnia(jadad score: 1). The other(RCT 2) was about urimic pruritus patients(jadad score: 5). In RCT 1, experimental group was significantly better than control group. In RCT 2, there was not statistical signification between two groups. Conclusions : In reality, there were not many papers on infrared therapy applications. Further study just like RCTs may be needed to establish evidenced-based guideline on infrared.

• Korean Journal of Acupuncture
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• v.32 no.3
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• pp.81-89
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• 2015

Objectives : The purpose of this study is to explore the trends of acupoints used for lumbar HIVD(Herniated Intervertebral Disc) and treatment and anatomical location of Hwatahyeopcheock. Methods : We searched the latest clinical studies on acupuncture treatment for lumbar HIVD(Herniated Intervertebral Disc) through domestic studies search. To analyze the data, we categorized them by year and article types of literatures and investigated often-used acupoints and acupuncture types for treatment by reading treatment part of every paper searched. Domestic and Chinese literatures related to Hwatahyeopcheock were also studied for its anatomical location. Results : Total 50 articles are searched and local points are more used than distant points for lumbar HIVD(Herniated Intervertebral Disc) and most of them showed curative effects. Back-su points were used the most and Hwatahyeopcheock use accounted for one-third of the articles. In the articles of Hwatahyeopcheock use, except for one, patients complained of both low back pain and radiating pain, and all of them showed positive results after treatment. Conclusions : Back su point is considered to have its meaning as physical region where patients feel pain including herniated disc level rather than the effect meridian system makes. Through document research and meridian muscle theory, we found that Hwatahyeopcheock means vertebral facet joint, intervertebral foramen and surrounding muscle, nerve, blood vessel and related spinal nerves.