• Journal of Microbiology
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• v.40 no.2
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• pp.140-145
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• 2002

The 5.8 kb pMMH1, rolling-circle replication (RCR) plasmid of the wild type soil Bacillus mesentericus was developed into a novel secretion vector system in Bacillus subtilis. The pMMHl turned out to have a replication origin and two open reading frames (ORFs) of the putative γ-GTP and type I signal peptidase (sipP). To characterize the regions necessary for plasmid stability and high copy number, five vectors (pPS, pPP, pEN, pMN, pME) were constructed by disruption or deletion of each region in pMMH1. Like pMMHl all constructed vectors were stable over 100 generations In a non-selective medium. Since pPS was the smallest (2.3 kb)of all, it was selected for the construction of a navel secretion vector, Using the $\alpha$-amylase promoter/signal sequence of B. subtilils the novel plasmid pJSN was constructed. When $\beta$-glucosidase was expressed using pJSN, we found $\beta$-glucosidase activity in the medium. This result strongly suggested that plasmid pJSN can be used for the production of bioactive peptides in B. subtilis.

• Korean Journal of Microbiology
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• v.50 no.3
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• pp.210-215
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• 2014

Pseudomonas nitroreducens TX1 was isolated from a rice field drainage in Taiwan. The bacterium is of special interest because of its capability to use a group of nonionic surfactants such as alkylphenol polyethoxylates even at high concentrations as a sole carbon source. In this study, a small cryptic circular plasmid, pTX1, was characterized from P. nitroreducens TX1. It is 2,286 bp in length with a GC content of 63.3% and harbors three open reading frames, $Rep_{pTX1}$ and functionally unidentified ORF1 and ORF2. The predicted $rep_{pTX1}$ gene product is homologous to Rep proteins of plasmids belonging to the pC194/pUB110 family, which is predominantly found in Gram-positive bacteria and is known to replicate by the rolling-circle mechanism. The copy number of pTX1 was estimated to be about 150 in each cell. Based on the genetic fingerprints and comparison with other plasmids, it is concluded that pTX1 replicates by a rolling circle mechanism which is rarely found for Pseudomonas plasmids.

• Journal of Microbiology and Biotechnology
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• v.13 no.3
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• pp.457-462
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• 2003

The complete nucleotide sequence of a plasmid, pMG1, isolated from Bifidobacterium longum MG1 has been determined. This plasmid, composed of 3,862 base pairs with 65.1% of G+C content. harbors two major open reading frames (ORF) encoding putative proteins of 29 kDa (ORF I) and 71 kDa (ORF II). ORF I showed relatively high amino acid sequence homology with replication proteins of other plasmids from Gr Im-positive and -negative bacteria. Upstream of ORF I, four sets of tandem repeat sequences resembling the iteron structure of related plasmids were found. S1 endonuclease treatment and Southern blot analysis revealed that pMG1 accumulates single-stranded DNA (ssDNA) intermediate, which indicate i the rolling circle replication (RCR) mechanism of this plasmid. Homology search indicated that ORF II encodes plasmid mobilization protein, and the presence of highly conserved oriT sequence in the upstream of this gene supported this assumption. RT-PCR showed that only ORF I is expressed in vivo. Based on these results, pMG 1 was exploited to construct a shuttle vector, pBES2. It was successfully transformed into Bifidobacterium and maintained stably.

• Journal of Korean Society for Atmospheric Environment
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• v.13 no.2
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• pp.115-122
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• 1997

Diurnal variation of $PM_{10}$ concentration was investigated for 24 sites in library including reading room, bookstock room, lobby, office and so on, and 9 sites in student building at Kangwon National University using scattering light integrated type digital dust indicator. The dimensional conversion factors for mass concentrations (K) were determined by comparing between the $PM_{10}$ concentrations collected by $PM_{10}$ hig volume air sampler and the digital dust indicator. The dimensional conversion factor (K) was 3.33 for indoor air in this experiment. The highest concentrations were 649 $\mug/m^3$/day for smoking room in the library and 242 $\mug/m^3$/day for circle room in the student building. Most of spaces in the library except a office and bookstock rooms were over 150 $\mug/m^3$/day of $PM_{10}$ concentrations and 6 indoor spaces were over the guideline for indoor air quality in student building except a health center and two restaurants. Therefore, it should be required to make an improvement on the indoor air quaility for public facilities in the university buildings.

• Journal of Families and Better Life
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• v.23 no.3 s.75
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• pp.17-24
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• 2005

This research examined the residents' behavior in using space, focusing on the kitchen area where there was the most change, in order to understand the transition of living culture through the ages. The following findings were obtained: 1) Today's apartment residents prefer 'Island' style which is located a separate counter and work space. Thus the kitchen m is now being recognized as a place spacious, and where the whole family can participate. 2) The usage of the kitchen is not only limited to cooking or keeping household goods but also broadened to washing, private life, happy home circle, inviting guests etc. Especially, family union or inviting guests in the kitchen is more frequent as the houses become larger, private refreshment or reading in the kitchen is proportional to income and to people living in homes where the kitchen and dining room is divided independent 3) Looking at the dietary life styles of today's apartment residents, there are more well-being type and tradition type than prosessed type. This trend is portional to the resident's standard of education and the size of the apartment, thus making a significant influence.

• English & American cultural studies
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• v.18 no.1
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• pp.93-120
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• 2018

Constance Fenimore Woolson is among those whom scholars have for long been trying to rediscover and add to the list of representative American writers. The primary methodology has been regionalism, based on the fact that most of her work portrays remote, exotic regions in and out of America. Still, Woolson remains obscure to general readers as well as literary critics outside a small circle of her scholarship. This essay attributes that obscurity to Woolson scholars' blind reliance on regionalism's nationalistic assumption in reading Woolson's multifaceted writing, and proposes to explore her nationally and regionally displacing view of the rigidly stereotypical and ideologically biased binary opposition between the center and the margin in postbellum America. The essay takes as an example the only story by Woolson that has never been reprinted or anthologized until very recently, "In Sloane Street," and examines why it resists the scholarly endeavor to regional categorization. The examination especially focuses on how the story exposes the Americanizing conceptualization of the region and its limits. The essay concludes with an attention to the story's ending where Woolson abruptly yet deliberately introduces a form of almanac as the main character Gertrude's mode of daily record. The attention to that uniquely hybrid genre in the American literary tradition, which encompasses the public and the private, the universal and the local, sheds light on Woolson's authorial intention to deconstruct the Manichean view of literary regionalism.

• Journal of the Korean Society for Library and Information Science
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• v.20
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• pp.65-125
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• 1991

The study has tried to analize and appraise how did public library system under the Japanese imperialism affect the establishment and managemant of Korean public libraries. To achieve the purpose of the above-mentioned study, the contents of $\ulcorner$Japanese library statute$\lrcorner$ under Japanese imperialism and the current $\ulcorner$Korean library law$\lrcorner$ have been mutually compared, at the same time, the vestiage of Japanese imperialism in view of the establishment, personnel administration and reading systems have been concretely investigated, analyzed and compared. The conclusions obtained from the above are as followings. 1. In those days of the Korean Liberation, the situation of Korean public libraries was such as it under the Japanese rule and so, their names were only changed. However, as a part of its independent activities, the national library have once carried out the various programs such as the training of professional librarians, the establishment of the new classification schedule and the chief Librarian and deputy Librarian from the professional librarians in the office regulations, and they were well worth being the good examples for today's Korean library circle. Though the Goverment of the Republic of Korea had been formally established, the situation of the library circle was very dull owing to the Korean war for a long time. In 1963, $\ulcorner$The Korean library law$\lrcorner$ was promulgated, but the establishment of public libraries did not give satisfactory results because of the institutional fragility. In the 1980's the importance of library was embossed from the viewpoint of life-long education and the number of libraries was increased. However, there were still the remaining vestiges of Japanese library system in the practical library services. 2. After the Korean Liberation, the influnces of public library system under the Japanese imperialism showed in the office regulation of national library and the Korea library Law were also in the legal mechanism. In particular, the regulations of $\ulcorner$The staff-member of public library$\lrcorner$ and $\ulcorner$Admission fee of public library$\lrcorner$ including the chief librarian have referred to the library system under the Japanese imperialism since the liberation day to date. 3. At that time of the Korean Liberation, the U.S.Military Government Office had decided that the public library administration should be attached to the administration of local and internal affairs in accordance with the Japanese administative system. As a result, the public libraries had been forced to be indirectly affected by public library system under the Japanese imperialism for twenty years since the Liberation. 4. Since the Liberation, the personnel adminstration of public library has been so far on the steps of model under the Japanese imperialism. As the result of the field survey, the position standards of local chief librarians, non-professional character, the extra post system and the preponderant appointment of non-professional offices have analyzed by the influence of Public library system under the Japanese imperialism. Therefore, the Government authorities-concerned must readjust the standards of qualification and the divided duties corresponding to the position of public library staff members and to stipulate expressly in the revised library law. In addition, the regulation of the admission fee should be also actively detected for the free adminssion of library users. 5. Since the Liberation Day, the reading methods of public library have been so far similar to reading method under the Japaness imperialism. For example, the admission fee levied, the complicated procedures of using books including entrance and exit of a library, no-admission system, the limited lending books, the deposit system of outdoor lending books and the surety liable jointly and severally are originally caused by bureaucracy of under the Japanese imperialism. Therefore, the public libraries should make an offer space and opportunities which can enjoy freedom to the gull in future. The procedures and standards of library users will be simplified, if possible. As the above-mentioned, the actual conditions of Korean public libraries have been examined and analyzed. As the result of it, there are still the remaining vestiges of public library system under the Japanese imperialism in the establishment and management of the nation-wide public libraries. Such the remnants are an obstacle to the democratic development of public libraries and so, the authorities-concerned should take the proper-measures as soon as possible.

• Science of Emotion and Sensibility
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• v.9 no.4
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• pp.341-351
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• 2006

The effect of color evokes a certain feeling and affects behavior. Especially, an understanding and applying color are critical because well-designed color substantially contributes to quality and usability. In this study, the effects of three color components(hue, value, and chroma) on apparent warmth were investigated separately and relatively, and then feasibility of applying to the information display was examined. The result showed that the apparent warmth of 10 Hues was a U-shaped function declining from Red to Purple-Blue and increasing from Purple-Blue to Red-Purple with following order of color-circle. Chroma made the character of hues remarkably clear, so warm color becomes warmer and cool color becomes cooler if chroma gets higher. But value has no effect on warmth. These results propose that we can change the apparent warmth by varying chroma in the limitation of color use. And there was a sharp distinction between warm and cool color, Meanwhile, in the reading task of the graphical information, the subjects' judgment distortion overestimating or underestimating the actual degrees was ascertained. This result should be applied to control operator's sensitivity in accordance with the purpose of task and display.

• Journal of Microbiology and Biotechnology
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• v.33 no.2
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• pp.211-218
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• 2023

A cryptic plasmid (pTH32) was characterized from Tetragenococcus halophilus 32, an isolate from jeotgal, Korean traditional fermented seafood. pTH32 is 3,198 bp in size with G+C content of 35.84%, and contains 4 open reading frames (ORFs). orf1 and orf2 are 456 bp and 273 bp in size, respectively, and their translation products showed 65.16% and 69.35% similarities with RepB family plasmid replication initiators, respectively, suggesting the rolling-circle replication (RCR) mode of pTH32. orf3 and orf4 encodes putative hypothetical protein of 186 and 76 amino acids, respectively. A novel Tetragenococcus-Escherichia coli shuttle vector, pMJ32E (7.3 kb, Em^r), was constructed by ligation of pTH32 with pBluescript II KS(+) and an erythromycin resistance gene (ErmC). pMJ32E successfully replicated in Enterococcus faecalis 29212 and T. halophilus 31 but not in other LAB species. A pepA gene, encoding aminopeptidase A (PepA) from T. halophilus CY54, was successfully expressed in T. halophilus 31 using pMJ32E. The transformant (TF) showed higher PepA activity (49.8 U/mg protein) than T. halophilus 31 cell (control). When T. halophilus 31 TF was subculturd in MRS broth without antibiotic at 48 h intervals, 53.8% of cells retained pMJ32E after 96 h, and only 2.4% of cells retained pMJ32E after 14 days, supporting the RCR mode of pTH32. pMJ32E could be useful for the genetic engineering of Tetragenococcus and Enterococcus species.

• Korean Journal of Heritage: History & Science
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• v.52 no.4
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• pp.264-271
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• 2019

Cheomseongdae in Gyeongju, known as an astronomical observatory, is a cultural monument with great historical, academic, and artistic value, as its unique shape is preserved well in its original form. The outer structure, ground stability, and seismic reliability of Cheomseongdae have been assessed by numerous researchers through various scientific methods, but research on the underground structure has been insufficient. This paper contains detailed models of the underground structure of Cheomseongdae interpreted in 2D and 3D images based on the data acquired through GPR surveys conducted of features in and around the base of Cheomseongdae. As a result, the existence of twelve small features arranged in a circle, although only about half of them remain, was confirmed at a depth of 0.4 - 0.6m. Furthermore, a structure three bays long (north-south direction) and four bays wide (east-west direction) was detected beneath Cheomseongdae at the depth of 0.7 - 1.0m. Other than 2 layers of foundations as is known, a square structure with the dimensions of 7m × 7m is situated at a depth of 0.6m, directly under Cheomseongdae, and what is reading that is expected to be the foundation structure of Cheomseongdae was detected and confirmed. This foundation structure is circular with a diameter in the east-west direction of 11m and in the north-south direction of 12m. The northern, western, and eastern edges of this foundation structure are about 1m away from the foundation of Cheomseongdae, whereas the the south side extends to about 5m wide.