• Fisheries and Aquatic Sciences
• /
• v.17 no.3
• /
• pp.325-337
• /
• 2014

To assess the functional structure of prepro-melanin-concentrating hormone (pMCH), we isolated and cloned pMCH (of-pMCH) mRNA from the brain of the olive flounder, Paralichthys olivaceus, and compared its amino acid sequence with those from other animals. In addition, to examine whether activation of the brain of-pMCH gene is influenced by background color, density, and feeding, we compared pMCH mRNA activities against different background colors (bright and dark) and at different densities (100% PCA and 200% PCA). To examine whether the pMCH gene is related with malpigmentation of blind-side skin and appetite, we compared pMCH gene expression between ordinary and hypermelanic flounders, and between feeding and fasting flounders. The of-pMCH cDNA was 405 bp in the open reading frame [ORF] and encoded a protein of 135 amino acids; MCH was 51 bp in length and encoded a protein of 17 amino acids. An obvious single band of the expected size was obtained from the brain and pituitary by RT-PCR. In addition, of-pMCH gene activity was significantly higher in the bright background only at low density (< 100% PCA) making the ocular skin of fish whitening, and in ordinary fish. However, the gene activity was significantly decreased in dark background, at high density (>200% PCA), and in hypermelano fish. These results suggest that skin whitening camouflage of the flounder is induced by high MCH gene activity, and the density disturbs the function of background color in the physiological color change. Moreover, our data suggest that a low level of MCH gene activity may be related to malpigmentation of the blind-side skin. In feeding, although pMCH gene activity was significantly increased by feeding in the white background, the pMCH gene activity in the dark background was not influenced by feeding, indicating that the MCH gene activity increased by feeding can be offset by dark background color, or is unaffected by appetite. In conclusion, this study showed that MCH gene expression is related to ocular-skin whitening camouflage and blind-skin hypermelanosis, and is influenced by background color and density.

• The Journal of the Korea Contents Association
• /
• v.15 no.11
• /
• pp.329-337
• /
• 2015

Fluorine is an element that promotes dental caries preventive effect at proper concentration level, but may have significantly undesirable impact on dental caries if correct information of fluorine is not recognized. Therefore, this study is intended to evaluate fluorine awareness among the adults in their 20s and analyze oral health conditions based on such awareness in order to promote dental caries preventive effect and convey correct knowledge of fluorine. For this study, 82 adult men and 102 adult women in their 20s, all of whom lived in Daegu, Gyeongsangbuk-do, were surveyed from May 1, 2014 to June 30 of the same year, along with oral health examination to evaluate their oral health conditions. The results of the analysis showed that 19.46% of respondents answered "No" to the question related to awareness towards the use of fluorine in DT rate dental clinic based on the degree of fluorine awareness, which was higher than 11.10% who answered "Yes"(p<.05). Moreover, 18.32%of respondents answered "No" to the question related to whether they were checking the label indicating the fluorine content in product, which was the highest rate, suggesting that the rate was higher when there was stronger tendency to avoid reading the precaution notice (p<.05). In addition, 71.98% of respondents answered "Yes" to the question associated with the awareness to the use of fluorine in FT rate dental clinic based on the fluorine awareness, which was higher than 49.04% of respondents who answered "No" (p<.05). This study is meaningful in that it provides basic data for the implementation of fluorine prevention projects in the period ahead by determining the effect of fluorine awareness on oral health conditions. Based on aforesaid results, both educational and promotional activities need to be carried out vigorously to help raise fluorine awareness.

• Journal of Microbiology
• /
• v.44 no.3
• /
• pp.301-310
• /
• 2006

Two pathways of ammonium assimilation and glutamate biosynthesis have been identified in microorganisms. One pathway involves the NADP-linked glutamate dehydrogenase, which catalyzes the amination of 2-oxoglutarate to form glutamate. An alternative pathway involves the combined activities of glutamine synthetase, which aminates glutamate to form glutamine, and glutamate synthase, which transfers the amide group of glutamine to 2-oxoglutarate to yield two molecules of glutamate. We have cloned the large subunit of the glutamate synthase (GOGAT) from Salmonella typhimurium by screening the expression of GOGAT and complementing the gene in E. coli GOGAT large subunit-deficient mutants. Three positive clones (named pUC19C12, pUC19C13 and pUC19C15) contained identical Sau3AI fragments, as determined by restriction mapping and Southern hybridization, and expressed GOGAT efficiently and constitutively using its own promoter in the heterologous host. The coding region expressed in Escherichia coli was about 170 kDa on SDS-PAGE. This gene spans 4,732 bases, contains an open reading frame of 4,458 nucleotides, and encodes a mature protein of 1,486 amino acid residues (Mr =166,208). The EMN-binding domain of GOGAT contains 12 glycine residues, and the 3Fe-4S cluster has 3 cysteine residues. The comparison of the translated amino acid sequence of the Salmonella GOGAT with sequences from other bacteria such as Escherichia coli, Salmonella enterica, Shigella flexneri, Yersinia pestis, Vibrio vulnificus and Pseudomonas aeruginosa shows sequence identity between 87 and 95%.

• Journal of Korean Library and Information Science Society
• /
• v.51 no.3
• /
• pp.81-102
• /
• 2020

The purpose of this study is to examine the students' and teachers' perception about the roles and qualifications of teacher librarians. For this purpose, data were collected through survey from students and teachers at secondary schools and the data were analyzed by semantic network analysis. The results of the research are as follows: First, students usually perceived the role of teacher librarians as 'library management', and teachers did as 'reading education'. Second, among the roles of teacher librarians, it was confirmed that students' and teachers' perceptions of 'information literacy instruction and library instruction' were very weak. Third, while the students' perception of the role of a teacher librarian as a 'teaching collaborator' such as 'teaching and learning support' and 'library assisted instruction and collaborative instruction' was weak, teachers recognized the role of teacher librarians as 'teaching collaborators'. Fourth, students and teachers perceived high levels of 'information service', which consists of 'book recommendation and guide activities'. Finally, it was investigated that 'professionalism' plays a central role in the students' and teachers' perception about the qualities of teacher librarians. These results can be used to establish the role of teacher librarians, develop response strategies for students and teachers, and improve their awareness.

• Korean Journal of Family Social Work
• /
• no.61
• /
• pp.93-136
• /
• 2018

The purpose of this study is to find specific practice methods of single parents self-help groups in community welfare centers. A participatory action research was conducted to achieve the purpose. Six social workers and three researchers participated in the study who shared the problem of lack of specific practice methods even though a self-help group was useful for self-reliance and empowerment of low-income single-parents. Various activities such as reading articles, analysis of each self-help group, case studies, and learning facilitation methods were conducted during 10 meetings. At the same time, peer supervision proceeded at each meeting. The progress of each group was shared, the application of practice methods and plans were discussed, and applying discussed methods to group practice and reviewing the process and outcomes of the practice were repeated in the peer supervison. As a result, the following positive changes appeared: First, the goals and vision of each group became clearer. Second, initiative of the members was promoted. Third, intimacy, mutual cooperation, the attatchment to the group were strengthened. A qualitative analysis was also conducted on the content discussed. As a result, 17 practice methods were derived in four themes such as initiative/ empowerment, cohesion, leadership, and destandardization. Practice issues concerning single parents self-help groups in community welfare centers were discussed based on the results.

• Journal of Korean Philosophical Society
• /
• v.145
• /
• pp.119-141
• /
• 2018

This paper is to address the consonance between Confucianism and Christianity, focusing on the first-generation clergy in Andong, North Gyeongsang Province, Korea. Andong has turned out many Confucian scholars since Yi Hwang, and thus founded the school of Togye (Yi Hwang's nom de plume). Meanwhile, some of Confucians in Andong converted to Christianity after reading the Bible. Thus, their religious activities and their interpretation of the Bible Confucian factors smacked of Confucianism, which suggests a need to reestablish the relationship between Confucianism and Christianity. This study produced the following results: First, the converts did not aspire to Christianity itself but to saving their country. The first-generation clergy in Andong wanted to sublimate their patriotic and Confucian spirits into Christianity, which is backed up by the independence movement planned jointly by Confucians and Christians in Andong. As for Confucians in Andong, their devotion to their country was a cause of the conversion to Christianity. Second, it was small wonder that Christianity was harmonious with Confucianism at least for early Christianity in Andong. In those days, Confucians in Andong had to achieve their realistic goals and thus did not need to deny Christianity. To relieve the national suffering, they had more need of Christian religiosity than Confucian morality. Likewise, missionaries wanted to propagate Christianity, and therefore did not deny the worldliness of Confucianism. On this wise, the two kept their identities and at the same time could compensate for their shortcomings from each other. This study names such Korean Christianity a 'humanistic religion.'

• Animal Bioscience
• /
• v.34 no.5
• /
• pp.867-879
• /
• 2021

Objective: Fibronectin 3 (FN3) and immunoglobulin like modules (Ig) are usually collocated beside modular cellulase catalytic domains. However, very few researches have investigated the role of these modules. In a previous study, we have sequenced and analyzed bacterial metagenomic DNA in Vietnamese goats' rumen and found that cellulase-producing bacteria and cellulase families were dominant. In this study, the properties of modular cellulases and the role of a FN3 in unique endoglucanase belonging to glycosyl hydorlase (GH) family 5 were determined. Methods: Based on Pfam analysis, the cellulases sequences containing FN3, Ig modules were extracted from 297 complete open reading frames (ORFs). The alkaline, thermostability, tertiary structure of deduced enzymes were predicted by AcalPred, TBI software, Phyre2 and Swiss models. Then, whole and truncated forms of a selected gene were expressed in Escherichia coli and purified by His-tag affinity column for assessment of FN3 ability to enhance enzyme activity, solubility and conformation. Results: From 297 complete ORFs coding for cellulases, 148 sequences containing FN3, Ig were identified. Mostly FN3 appeared in 90.9% beta-glucosidases belonging to glycosyl hydrolase family 3 (GH3) and situated downstream of catalytic domains. The Ig was found upstream of 100% endoglucanase GH9. Rarely FN3 was seen to be situated downstream of X domain and upstream of catalytic domain endoglucanase GH5. Whole enzyme (called XFN3GH5 based on modular structure) and truncate forms FN3, XFN3, FN3GH5, GH5 were cloned in pET22b (+) and pET22SUMO to be expressed in single and fusion forms with a small ubiquitin-related modifier partner (S). The FN3, SFN3 increased GH5 solubility in FN3GH5, SFN3GH5. The SFN3 partly served for GH5 conformation in SFN3GH5, increased modules interaction and enzyme-soluble substrate affinity to enhance SXFN3GH5, SFN3GH5 activities in mixtures. Both SFN3 and SXFN3 did not anchor enzyme on filter paper but exfoliate and separate cellulose chains on filter paper for enzyme hydrolysis. Conclusion: Based on these findings, the presence of FN3 module in certain cellulases was confirmed and it assisted for enzyme conformation and activity in both soluble and insoluble substrate.

• Journal of Korean Library and Information Science Society
• /
• v.50 no.1
• /
• pp.357-380
• /
• 2019

The purpose of this study is to analyze the Elementary and Secondary Education Act(ESEA) of the U.S. reauthorized by the Every Student Succeeds Act in 2015 from the viewpoint of the library and information services, and to derive implications for improving the library and information services. For the first time, ESEA includes effective school library programs and school librarians, and links school library programs and school librarians with literacy, digital literacy, books, resources, up-to-date materials, technology, library services and educational services. It provides a financial and institutional base for library and information services in elementary and secondary schools of the US to be more conducted. In addition, school librarians are required to participate in personalized learning experiences, evidence-based assessments, and professional development in the law, so school librarians must provide library and information services to students, staff, and parents in order to improve student achievement and digital literacy. Based on these analyses, this study discussed strengthening access to the school library, specifying the work of the teacher-librarian's library and information services, strengthening collaboration with school members, educational activities based on evidence base, sharing educational effects and developing of library and information curriculum.

• 한국노년학
• /
• v.36 no.3
• /
• pp.785-808
• /
• 2016

This study portraits the way of self-preparation for death among the elderly and examines the meaning and values attached to the elderly-care by their family and friends through the analysis of Kyung-ran Jo's novels. First, the author describes the difficulties and inner conflict of women who provide care for the elderly, especially those facing death in the near future. She also describes how the acceptance of death and self-discovery among the caregivers can be developed from the experience of long term care. These are consistent with the arguments by Ecofemnist on positive sides of providing caregiving in an aging society. In her novels, caregiving from young women is not described as the consequences of internalized gender norms of traditional Confucian culture. Rather, it is depicted as attitude toward a life in a commune where the meaning of life and death of elderly is shared between generations. In addition, struggles of the elderly figures against diseases and the process of their death provide an opportunity for young caregivers to overcome the trauma of other's death witnessed in their childhood. They lead the young caregivers to reflect themselves by raising ontologistic questions on life and death. The author shows the communication between generations presenting the self-preparation of death from the old generation undergoing irreversible aging and caregiving activities of significant others from the young generation. In doing so, the author points out the ethical grounds for diseases and death in old age given the condition of personalization of death in the modern society.

• Journal of The Korean Association of Information Education
• /
• v.23 no.3
• /
• pp.255-264
• /
• 2019

Modern society has become a key factor in determining software competitiveness. Therefore, Korea has required more than 17 hours of software education per year in the actual course subject to the 2015 revised curriculum. In this paper, we analyzed the software related units in 6 kinds of textbooks of elementary school published based on '2015 revised curriculum' and tried to provide basic data for selection of textbooks related to software education in elementary school. As a result of the analysis, the 6 revised textbooks of 2015 appropriately reflected both 'understanding of software', 'procedural problem solving', 'contents of programming element and structure', and I was suggesting appropriate activities. Unit support materials use comics and illustrations to stimulate interest, supplement text, and deepen learning. Four kinds of textbooks provide additional information by presenting reading materials. However, in most textbooks, the proportion of learning using the appendix was low. Although it consists of units focused on knowledge understanding and practice, it can be a textbook that enhances students' interest and participation if they are made of software in daily life, problem solving by procedural thinking, and so on.