• Title/Summary/Keyword: reactivation

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Reactivation of Horseradish Peroxidase in Organic Media Using Solvent Evaporation (용매 증발에 의한 유기용매내 Horseradish peroxidase의 재생)

  • Choe, Yu-Seong;Yu, Yeong-Je
    • KSBB Journal
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    • v.14 no.4
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    • pp.465-469
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    • 1999
  • Horseradish peroxidase(HRP) in organic solvent can be reactivated by evaporation. In order to measure the evaporation effect, the enzyme solutions were obtained by evaporation and dilution of organic solvent, respectively. Although two situations were thermodynamically identical, the activity from evaporation was higher than that from dilution. From the UV absorbance and the fluorescence intensity mesurements, it can be explained that reactivation of enzyme activity might be caused by reversible folding, and the enzyme obtained by evaporation was more refolded than that obtained by dilution.

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The Contribution of Pre-Existing Structures during the Structural Inversion in Cretaceous Sedimentary Rocks on Geoje Island, SE Korea

  • Francois Hategekimana;Mohammed S. M. Adam;Young-Seog Kim
    • Journal of the Korean earth science society
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    • v.44 no.4
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    • pp.275-290
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    • 2023
  • Structural inversion refers to the reverse reactivation of extensional faults that influence basin shortening accommodated by contractional faults or folds. On the Korean peninsula, Miocene inversion structures have been found, but the Cretaceous rocks on Geoje Island may have undergone inversion as early as the Upper Cretaceous. To evaluate the structural inversion on Geoje Island, located on the eastern side of South Korea, and to determine the effects of preexisting weakness zones, field-based geometric and kinematic analyses of faults were performed. The lithology of Geoje Island is dominated by hornfelsified shale, siltstone, and sandstone in the Upper-Cretaceous Seongpori formation. NE and NW-oblique normal faults, conjugate strike-slip (NW-sinistral transpressional and E-W-dextral transtensional) faults, and NE-dextral transpressional faults are the most prominent structural features in Geoje Island. Structural inversion on Geoje Island was evidenced by the sinistral and dextral transpressional reactivation of the NW and NE-trending oblique normal faults respectively, under WNW-ESE/NW-SE compression, which was the orientation of the compressive stress during the Late Cretaceous to Early Cenozoic.

Effect of Pore Structure Change on the Adsorption of NOM and THMs in Water Due to the Increase of Reactivation Number of Coal-based Activated Carbon (석탄계 활성탄의 재생 횟수 증가에 따른 세공 구조 변화가 수중의 NOM과 THM 흡착에 미치는 영향)

  • Son, Hee-Jong;Ryu, Dong-Choon;Jang, Seong-Ho
    • Journal of Korean Society of Environmental Engineers
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    • v.32 no.10
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    • pp.965-972
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    • 2010
  • The objective of this research was to evaluate for the changes of pore structures and adsorption capacities due to the increase the numbers of reactivation. The reactivated GAC had experienced three cycles of water treatment and thermal reactivation. The pore size distributions of virgin and reactivated GACs were very different. The virgin GAC was mostly microporous (< $15\;{\AA}$), with less mesopores ($20{\sim}100\;{\AA}$). The reactivated GACs was mostly mesoporous ($20{\sim}100\;{\AA}$), with less micropores (< $15\;{\AA}$). The specific surface area and total pore volume were reduced as the number of reactivation increased. The maximum adsorption capacity (X/M) of virgin GAC ($964.6\;{\mu}g/g$) for $CHCl_3$ was 2~3 times larger than 1st~3rd reactivated GAC ($255.6{\sim}399.5\;{\mu}g/g$). The maximum adsorption capacity (X/M) of virgin GAC (19.5 mg/g) for DOC (dissolved organic carbon) was equal to that of 1st~3rd reactivated GAC (18.0~18.7 mg/g).

Potency of Several Structurally Different Acetylcholinesterase Reactivators to Reactivate House Fly and Bovine Acetylcholinesterases Inhibited by Paraoxon and DFP

  • Park, No-Jung;Jung, Young-Sik;Musilek, Kamil;Jun, Daniel;Kuca, Kamil
    • Bulletin of the Korean Chemical Society
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    • v.27 no.9
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    • pp.1401-1404
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    • 2006
  • Eight structurally different acetylcholinesterase reactivators derived from currently commercially available oximes were tested for their potency to reactivate acetylcholinesterase inhibited by pesticide paraoxon (P) and DFP (D). Housefly AChE (F) and bovine red blood cell AChE (B) were used as the source of the cholinesterases. Ellman's method was taken to examine cholinesterases activity. The results show that four AChE reactivators are potent AChE reactivators, able to reach reactivation potency of more than 30% in all cases - PF, PB, DF and DB. Their reactivation potency was comparable with that of pralidoxime and even higher compared with that of HI-6, standard AChE reactivators currently available on the market.

Discussion on the Quaternary fault movement at Yugye-ri area in the northern part of Yangsan Fault (양산단층 북부 유리계 일대의 신기 단층운동 소고)

  • 경재복
    • Proceedings of the Earthquake Engineering Society of Korea Conference
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    • 2001.04a
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    • pp.66-73
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    • 2001
  • Yangsan fault in the southeastern part of Korean peninsula has been locally reactivated along a prexisted fault during the late Pleistocene time. Geomorphological evidence of the reactivation is revealed at the northern segment(Yugye-ri, Tosung-ri areas) of the Yangsan fault. The reactivation is distinctively characterized by fault gouge and fracture zone with high frequency in the Yugye-ri area. Obique slip separation of the area is about three meters of the middle terrace. The cumulative vertical displacement is recognized after the formation of the middle terrace. Age of the reactivated faulting is constrained to during the formation of dissected valley deposits. Average vertical slip based on paleo-event is inferred to about 0.5-0.7 meter in this area.

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Development of Parallel TBR system for the treatment of Trichloroethylene by Burkholderia cepacia G4

  • Lee, Eun-Yeol;Ye, Byeong-Dae;Park, Seong-Hun
    • 한국생물공학회:학술대회논문집
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    • 2000.11a
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    • pp.512-515
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    • 2000
  • A parallel reactor system which is consisted of two trickle bed reactors (TBR) was developed for the biodegradation of trichloroethylene (TCE) in waste gas stream. The reactor were packed with porous ceramic materials and Burkholderia cepacia G4 was inoculated to form biofilms. Each reactor was operated alternatively in TCE degradation or reactivation mode, and the effect of switching time on TBR performance was investigated. The MO (monooxygenase) activity during the TCE transformation decreased below 10 % within 24 hr, but could be recovered to the initial high level within 10 hr after supplying the reactivation medium supplemented with phenol as a carbon source. This shows that the parallel TBR system has a great potential for the long-term stable treatment of TCE.

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Studies on Inactivation and Reactivation of Isolated Photosystem II Complexes in Spinach (시금치에서 분리한 광계 2 복합체의 불활성화와 재활성화에 대한 연구)

  • 전현식
    • Journal of Plant Biology
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    • v.33 no.4
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    • pp.277-283
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    • 1990
  • Inactivation and reactivation of photosynthetic oxygen evolving complex were studied with isolated spinach (Spinacia oleraceda. L.) photosystem II particles by the activity of oxygen evolution and chlorophyll fluorescence. When the particles were treated with Tris and urea, the oxygen evolution was inactivated and three polypeptides having molecular weights of 33 kDa, 24 kDa and 18 kDa were simultaneously released. But in NaCl-treated particles, two polypeptides of 24 kDa and 18 kDa were removed from PS II particles. The oxygen evolution activities of Tris and urea-treated particles were not restored by adding cation ions (Mg2+, Mn2+ and Ca2+), but the NaCl-treated particles were restored by exogenously added Ca2+. The removal of these extrinsic polypeptides, especially 33 kDa, markedly showed the decrease of the variable fluorescence (Fv). These results are likely to be due to dissipate thermal energy by antenna of photosystem II complexes.

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Herpes Zoster and Postherpetic Neuralgia: Practical Consideration for Prevention and Treatment

  • Jeon, Young Hoon
    • The Korean Journal of Pain
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    • v.28 no.3
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    • pp.177-184
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    • 2015
  • Herpes zoster (HZ) is a transient disease caused by the reactivation of latent varicella zoster virus (VZV) in spinal or cranial sensory ganglia. It is characterized by a painful rash in the affected dermatome. Postherpetic neuralgia (PHN) is the most troublesome side effect associated with HZ. However, PHN is often resistant to current analgesic treatments such as antidepressants, anticonvulsants, opioids, and topical agents including lidocaine patches and capsaicin cream and can persist for several years. The risk factors for reactivation of HZ include advanced age and compromised cell-mediated immunity (CMI). Early diagnosis and treatment with antiviral agents plus intervention treatments is believed to shorten the duration and severity of acute HZ and reduce the risk of PHN. Prophylactic vaccination against VZV can be the best option to prevent or reduce the incidence of HZ and PHN. This review focuses on the pathophysiology, clinical features, and management of HZ and PHN, as well as the efficacy of the HZ vaccine.

In vitro Screening of Oxime Reactivators on the Model of Paraoxon-inhibited Acetylcholinesterase-SAR Study

  • Holas, Ondrej;Musilek, Kamil;Pohanka, Miroslav;Kuca, Kamil;Opletalova, Veronika;Jung, Young-Sik
    • Bulletin of the Korean Chemical Society
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    • v.31 no.6
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    • pp.1609-1614
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    • 2010
  • Acetylcholinesterase reactivators are crucial antidotes for the treatment of organophosphate intoxication. Standard in vitro test was chosen using a rat brain homogenate as the source of AChE. Screening of reactivation potency was performed with two concentration of reactivator (1000 ${\mu}M$ and 10 ${\mu}M$). Results were compared to established reactivators pralidoxime, methoxime, HI-6, trimedoxime and obidoxime. More than 30 novel reactivators performed equal or better reactivation ability of POX-inhibited AChE compared to currently used reactivators. The structure-activity relationship for reactivators of paraoxon-inhibited AChE was developed.

Mandibular osteonecrosis following herpes zoster infection in the mandibular branch of the trigeminal nerve: a case report and literature review

  • Song, Jae-Min;Seo, Jeong-Seok;Lee, Jae-Yeol
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.41 no.6
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    • pp.357-360
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    • 2015
  • Herpes zoster virus (HZV) infections are caused by reactivation of the varicella zoster virus. Reactivation symptoms commonly affect the thoracolumbar trunk, and rarely affect the mandibular branches of the trigeminal nerve. When the mandibular branches are involved, lesions appear proximal to the innervation area. This condition may be associated with exfoliation of the teeth and osteonecrosis of the jawbone. We report a case of mandibular osteomyelitis after herpes zoster infection and we present a review of the literature on mandibular-branch involvement of HZV-related osteonecrosis.