• Title/Summary/Keyword: rbcS

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Effects of Dietary Lactobacillus brevis Supplementation on Growth Performance, Dry Matter and Nitrogen Digestibilities, Blood Cell Counts and Fecal Odor Emission Compounds in Growing Pigs (육성돈사료에 Lactobacillus brevis의 첨가가 성산성, 건물과 질소 소화율, 혈구수 및 분 내 악취 발생 물질에 미치는 영향)

  • 진영걸;민병준;조진호;김해진;유종상;김인호
    • Journal of Animal Science and Technology
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    • v.48 no.4
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    • pp.503-512
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    • 2006
  • This study was conducted to investigate the effects of dietary Lactobacillus brevis (3.4×108 CFU/g) supplementation on growth performance, DM and N digestibilities, blood cell counts and fecal odor emission compounds in growing pigs. Ninety six crossbred [(Landrace×Yorkshire)×Duroc] pigs with an initial BW of 24.60±1.28kg were used for 42-d feeding trial according to a completely randomized design. Three corn- soybean meal based dietary treatments included: 1) CON (basal diet); 2) LB1 (basal diet + Lactobacillus brevis 0.2%) and 3) LB2 (basal diet+Lactobacillus brevis 0.4%). There were three dietary treatments with eight replicate pens per treatment and four pigs per pen. Through the entire experimental period, ADG, ADFI and gain/feed had no significant differences among treatments(P>0.05). Nitrogen digestibility was increased in LB1 and LB2 treatments compared to CON treatment (linear effect, P<0.05), however, DM digestibility had no significant difference among all the treatments (P>0.05). The WBC, RBC and lymphocyte concentrations in whole blood were not affected by treatments (P>0.05). Fecal NH3N and H2S concentrations were significant decreased in LB2 treatment compared to CON treatment (linear effect, P<0.05). Fecal VFA (acetic acid and propionic acid) concentration was also reduced in LB2 treatment compared to CON treatment (linear effect, P<0.05). In conclusion, Lactobacillus brevis (3.4×108 CFU/g) supplementation at the level of 0.4% can improve nitrogen digestibility and decrease the concentrations of fecal odor emission compounds in growing pigs.

Characterization of Nitric Oxide (NO)-Induced Cell Death in Lung Epithelial Cells (폐상피세포에서 Nitric Oxide (NO)에 의한 세포사에 관한 연구)

  • Yong, Wha Shim;Kim, Youn Seup;Park, Jae Seuk;Jee, Young Koo;Lee, Kye Young
    • Tuberculosis and Respiratory Diseases
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    • v.56 no.2
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    • pp.187-197
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    • 2004
  • Background : Nitric Oxide (NO) is a multi-faceted molecule with dichotomous regulatory roles in many areas of biology. NO can promote apoptosis in some cells, whereas it inhibits apoptosis in other cell types. This study was performed to characterize NO-induced cell death in lung epithelial cells and to investigate the roles of cell death regulators including iron, bcl-2 and p53. Methods : A549 cells were used for lung epithelial cells. SNP (sodium nitroprusside) and SNAP (S-nitroso-N-acetyl- penicillamine) were used for NO donor. Cytoxicity assay was done by MTT assay and crystal violet assay. Apoptotic assay was done by fluorescent microscopy after double staining with propidium iodide and hoecst 33342. Iron inhibition study was done with RBCs and FeSO4. For bcl-2 study, bcl-2 overexpressing cells (A549-bcl-2) were used and for p53 study, Western blot analysis and p53 functionally knock-out cells (A549-E6) were used. Results : SNP and SNAP induced dose-dependent cell death in A549 cells and fluorescent microscopy revealed that SNAP induced apoptosis in low doses but necrosis in high doses while SNP induced exclusively necrotic cell death. Iron inhibition study using RBCs and FeSO4 significantly blocked SNAP-induced cell death. And also SNAP-induced cell death was blocked by bcl-2 overexpression. Finally, we found that SNAP activate p53 by Western blot analysis and that SNAP-induced cell death was decreased in the abscence of p53. Conclusion : In lung epithelial cells, NO can induce cell death, more precisely apoptosis in low doses and necrosis in high doses. And iron, bcl-2, and p53 play important roles in NO-induced cell death.

Hematological Reference Values in the Healthy Adults (건강성인의 혈구 참고치 산정)

  • Kim, Young-Jin;Hyun, Myung-Soo;Lee, Hyun-Woo
    • Journal of Yeungnam Medical Science
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    • v.8 no.1
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    • pp.154-165
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    • 1991
  • To establish the hematological reference values in the healthy adults visited our hospitals, following examination were done on 2823 persons by Coulter Counter Model S-plus II ; white blood cell count: (WBC), red blood cell count(RBC), hemoglobin(Hb), hematocrit(Hct), mean corpuscular volume (MCV), mean corpuscular hemoglobin(MCH), mean corpuscular hemoglobin concentration(MCHC), red cell distribution width(RDW), platelet, plateletcrit, mean platelet volume(MPV) and platelet distribution width(PDW). The following results are obtained. 1) Male, mean value of WBC ; $6,800{\pm}2,680(2SD)/{\mu}l$ Female, mean value of WBC ; $5,950{\pm}2,380(2SD)/{\mu}l$ 2) Male, mean value of RBC ; $428{\pm}60(2SD){\times}10^4/{\mu}l$ Female, mean value of WBC ; $415{\pm}56(2SD){\times}10^4/{\mu}l$ 3) Male, mean value of Hb ; $15.4{\pm}1.8(2SD)g/dL$ Female, mean value of Hb ; $13.0{\pm}1.6(2SD)g/dL$ 4) Male, mean value of Hct ; $45.3{\pm}5.0(2SD)%$ Female, mean value of Hct ; $38.2{\pm}4.6(2SD)%$ 5) Male, mean value of MCV ; $93.8{\pm}5.8(2SD)fL$ Female, mean value of MCV ; $92.2{\pm}7.4(2SD)fL$ 6) Male, mean value of MCH ; $31.8{\pm}2.2(250)pg$ Female, mean value of MCH ; $31.4{\pm}2.8(2SD)pg$ 7) Male, mean value of MCHC ; $34.0{\pm}1.2(2SD)%$ Female, mean value of MCHC ; $33.9{\pm}1.2(2SD)%$ 8) Male, mean value of RDW ; $12.7{\pm}1.0(2SD)%$ Female, mean value of RDW ; $12.6{\pm}1.4(2SD)%$ 9) Male, mean value of Platelet ; $242.9{\pm}87.8(2SD){\times}10^3/{\mu}l$ Female, mean value of Platelet ; $242.2{\pm}89.0(2SD){\times}10^3/{\mu}l$ 10) Male, mean value of Plateletcrit ; $0.201{\pm}0.076(2SD)%$ Female, mean value of Plateletcrit ; $0.204{\pm}0.076(2SD)%$ 11) Male, mean value of MPV ; $8.20{\pm}1.70(2SD)fl$ Female, mean value of MPV ; $8.36{\pm}1.82(2SD)fl$ 12) Male, mean value of PDW ; $16.1{\pm}0.8(2SD)%$ Female, mean value of PDW ; $16.0{\pm}0.8(2SD)%$.

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Effect of Temperature on Growth and Related Gene Expression in Alternative Type Wheat Cultivars (양절형 밀 생장에 대한 온도의 영향과 유전자 발현 양상)

  • Heo, Ji Hye;Seong, Hye Ju;Yang, Woon Ho;Jung, Woosuk
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.64 no.4
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    • pp.384-394
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    • 2019
  • We have investigated the effects of ambient temperature on the growth of wheat in Korea. The differences in the growth phase of wheat were compared according to the temperature treatment. The productive tiller number and dry weight were decreased in a plot under a higher temperature treatment. We found that the growth of Jinpum was different from that of the alternative wheat cultivars, which were bred in Korea, at 50 days after treatment. While the Jinpum wheat grown at 17℃ showed vegetative stage growth, that grown in the 23℃ growth chamber entered the heading and flowering stage. The differences in the expression of 16 genes known to be involved in high-temperature responses were checked by using Jinpum wheat 50 days after two temperature treatments (17℃ and 23℃), which showed apparent differences in expression between the higher and lower temperatures during the growth phase. In the 23℃ treatment samples, the genes with increased expression were HSP70, HSP101, VRN2, ERF1, TAA1, YUCCA2, GolS, MYB73, and Histone H2A, while the genes with decreased expression were VRN-A1, DREB2A, HsfA3, PIF4, PhyB, HSP17.6CII, rbcL, and MYB73. YUCCA2, HSP101, ERF1, and VRN-A1 showed a significant difference in gene expression between lower- and higher-temperature conditions. Overall, combining the means of the expression of various genes involved in thermosensing, vernalization, and abiotic stresses, it is possible to conclude that different sets of genes are involved in vernalization and summer depression of wheat under long term, high ambient temperature conditions.

Evaluation of the Antimicrobial Activities of 35 Seaweed Extracts against Pathogenic Bacteria and Candida sp. (35종 해조류 추출물의 병원성 세균 및 Candida sp. 진균에 대한 항균 활성 평가)

  • Kim, Mi-Sun;Kwon, Kyung-Jin;Lee, Min-Jin;Ahn, Seon-Mi;Sohn, Ho-Yong
    • Microbiology and Biotechnology Letters
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    • v.40 no.2
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    • pp.144-151
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    • 2012
  • In the course of this study aimed at the development of functional food ingredients from seaweeds, the in vitro antimicrobial activities of methanol extracts prepared from 35 different seaweeds (17 phaeophyta, 11 rhodophyta and 7 chlorophyta) were determined against food-borne diseases and pathogenic microorganisms including multi-drug resistant (MDR) Pseudomonas sp. and Candida sp. Based on disc-diffusion assays at 500 g/disc concentration of the methanol extracts, Ishige okamurai, I. foliacea, Sargassum confusum, and S. yamade exhibited strong antibacterial activities in a broad-spectrum, except against Pseudomonas aeruginosa. In addition to the latter four seaweeds, Ecklonia stolonifera, E. cava and Eisenia bicyclis also demonstrated antifungal activity against C. albicans. Among these 8 selected seaweeds, I. okamurai, I. foliacea, and S. yamade exhibited strong hemolytic activity (55-93%) at 500 g/ml against human RBC. Organic solvent sequential fractions using hexane, ethylacetate and butanol, and water residues were prepared from the 8 selected seaweeds and their anti-Candida sp. activities were further determined. The ethylacetate and butanol fraction of I. okamurai, and the hexane fraction of I. foliacea demonstrated antifungal activity against MDR-pathogenic Candida sp. Although the solvent fractions had no activity against MDR-Pseudomonas sp., our results suggest that seaweeds, especially Ishige okamurai, I. foliacea, S. confusum, and S. yamade could be developed as broad-spectrum antimicrobial ingredients.

[I-123]IPT SPECT Dopamine Reuptake Site Imaging: Differences in Normal Controls and Parkinson's Patients by Semiquantitative Analysis Methods ([I-123]IPT SPECT를 이용한 도파민 재섭취부위의 영상화: 반정성적 분석방법을 이용한 정상인과 파킨슨병 환자의 차이)

  • Kim, Hee-Joung;Im, Joo-Hyuck;Yang, Seoung-Oh;Ryu, Jin-Sook;Choi, Yun-Young;Lee, Myung-Chong;Lee, Hee-Kyung
    • The Korean Journal of Nuclear Medicine
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    • v.30 no.1
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    • pp.35-46
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    • 1996
  • Dopamine transporter concentrations have been known to decrease in Parkinson's disease (PD) or increase in Tourette's disorder. The purpose of this study was to evaluate the effectiveness of [I-123]N-(3-iodopropene-2-yl)-$2{\beta}$-carbomethoxy-$3{\beta}$-(4-chlorophenyl) tropane (IPT) as an imaging agent for measuring changes in transporter concentrations with PD. IPT labelled with 6.69+/-0.64 mCi(247.53+/-23.68 MBq) of I-123 was intravenously injected into ten patients(age: 55+/-11) with PD, and six normal controls(NC)(age: 46+/-14) as a bolus. Dynamic SPECT scans of the brain were then performed for 5 minutes each over 120 minutes on a triple headed camera. Time activity curves were generated for the left basal ganglia(LBG), right basal ganglia(RBC), and occipital cortex(OCC). The statistical parameters included the time to peak activity, the contrast ratio of LBG and RBG to OCC at several time points, and the accumulated specific binding counts/mCi/pixel(ASBC) from 0 to 115 minutes. The uptake of IPT in the brains of PD and NC peaked within 10 minutes of injection in all subjects. The maximum target to background ratio in the basal ganglia of PD and NC occurred at 85+/-20 min and 110+/-6 min of injection, respectively. The BG/OCC ratios at 115 minutes for PD and NC were 2.15+/-0.54 and 4.26+/-0.73, respectively. The ASBC at 115 minutes for PD and NC were 152.91+/-50.09 and 289.51+/-49.00, respectively. The ratio of BG/OCC for the NC was significantly higher than the ratio for PD. SPECT data matched with clinical diagnosis for PDs. The ratio between BG and OCC and the ASBC for PD were clearly separated from NC and may be useful outcome measures for clinical diagnosis. The findings suggest that IPT may be a very useful tracer for early diagnosis of PD and study of dopamine reuptake site.

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Effects of Dioxin on the Body Weight, No. of Sperm, Motility, Testis and Organ Weight in Mice (Dioxin의 투여가 마우스의 체중, 정자수, 정자활력, 정소 및 장기중량에 미치는 영향에 관한 연구)

  • 김상근;김민수;왕애국;남윤이;현병화
    • Korean Journal of Animal Reproduction
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    • v.24 no.3
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    • pp.231-239
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    • 2000
  • In this study, we observed the effects of dioxin on weights of body, testes and other organs, the number and motility of sperm in the a various dose after two days' of administration in mice. Animals were treated with oral dose of dioxin 10, 20, 30, 40 mg/kg body weight, respectively. 1, After administration dioxin at doses of 10, 20, 30 and 40 $\mu\textrm{g}$/kg to the mice, the changes in body weights were 30.6 $\pm$ 2.g~40.7 $\pm$ 3.9g and 30.8 $\pm$4.1g~39.5g $\pm$3.1 for 10 and 20 $\mu\textrm{g}$/kg dosed group, 31.0 $\pm$ 3.5g ~ 39.0 $\pm$ 3.5g, 30.6 $\pm$ 3.4g~38.3 $\pm$ 4.0g for 30 and 40 $\mu\textrm{g}$/kg dosed group. The body weight of dioxin-administered group showed lower value when compared to 30.6 $\pm$ 2.8g ~ 44.5 $\pm$ 3.1g of which is control group's. 2. After administration of dioxin at doses of 10, 20, 30 and 40 $\mu\textrm{g}$/kg to the mice, the increase in the number of WBC was prominent, but the increase in the number of RBC wasn't significant, though the values of Hb, PCV, and PLT were higher than those of control group's. 3. After administration of dioxin at doses of 10. 20, 30 and 40 $\mu\textrm{g}$/kg to the mice, the changes in sperm number were 112.5 $\pm$ 3.7~119.4 $\pm$4.2 $\times$ 10$^{6}$ $m\ell$, 103.9 $\pm$3.8 ~ 110.2 $\pm$ 3.6 $\times$ 10$^{6}$ $m\ell$, 97.5 $\pm$ 3.4 ~105.7 $\pm$ 4.4 $\times$ 10$^{6}$ $m\ell$, 87.2 $\pm$ 3.7~98.5 $\pm$ 3.8 $\times$ 10$^{6}$ $m\ell$, respectively. The sperm number of dioxin-administered group showed lower value than that of control group's, which was 119.0 $\pm$ 4.3 ~ 120.7 $\pm$ 4.8 $\times$ 10$^{6}$ $m\ell$. After administration of dioxin at doses of 10~40 $\mu\textrm{g}$/kg to the mice, the sperm motility were 69.4$\pm$ 3.0 ~ 86.6 $\pm$4.7%. The sperm motility of dioxin-administered group showed lower value than that of control group's. 4. After administration of dioxin at doses of 10, 20, 30 and 40$\mu\textrm{g}$/kg to the mice, the organ weight of each dioxin-administered group's was decreased a little compared to that of control group's. 5. After administration of dioxin at doses of 10, 20, 30 and 40 $\mu\textrm{g}$/kg to the mice, the weights of spleen, kidneys, and liver showed increase a little.

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A Study on the Statistical Characteristics of Precipitation & Temperature Data of Four Cities and the Statistical Criterion of Climate Change (우리나라 4개 도시의 강수량과 기온자료의 통계적 특성과 기후변화의 통계적 기준에 관한 연구)

  • 이상훈;장영기
    • Journal of Korean Society for Atmospheric Environment
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    • v.7 no.3
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    • pp.180-188
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    • 1991
  • 기후변화를 나타내는 연구 결과들은 기온이나 강수량을 대상으로 하여 두 기간 평균치의 비교, 5년 이동평균치의 圖示, 회귀분석 등의 방법들을 사용하여 발표되지만 대부분의 경우 밑바탕이 되는 모집단의 통계적 특성을 엄격히 검토하지 않는 정성적인 분석인 경우가 많다. 서울, 부산, 인천, 목포 등 4개 지점의 80년 동안의 연강수량과 연평균기온자료를 분석한 결과 기온은 정규분포를 나타내므로 전통적인 t-검정과 회귀분석을 적용할 수 있다. t-검정 결과 두 기간(1920 $sim$ 1949, 1961 ~ 1990)은 연평균기온에서 0.3$^{\circ}$C에서 $0.8^{\circ}$C의 의의있는 차이를 보였다. 최근 30년의 연평균기온자료를 회귀분석한 결과 1년에 $0.02^{\circ}$C의 증가경향을 나타내었다. 연강수량은 정적인 왜곡도를 보이므로 정규분포를 가정하는 母數的 통계분석을 적용해서는 안되며 비모수적 통계분석법을 적용해야 한다. 연강수량의 분석에는 t-검정에 상응하는 Mann-Whitney 검정을 적용해 본 결과 두 기간(1920 ~ 1949, 1961 ~ 1990)의 평균은 통계적으로 의의있는 차이가 없었다. 회귀분석에 상응하는 Mann's 검정과 이 연구에서 새로이 제안된 Median Slope Change Estimator를 적용해 본 결과 역시 통계적으로 의의있는 변화가 없었다. Median Slope Change Estimator는 비모수적 통계치로서 회귀분석의 기울기에 해당하는 연변화를 나타낼 수 있는데 분석하려는 자료의 정규분포성을 요구하지 않으며 異常點(outlier) 영향에 덜 민감하다는 장점이 있으므로 주목하여 연구할 만한 가치가 있다.^{\circ}$~40$^{\circ}$ 구간에서는 ${\sigma}_c$와 I$_{sa}$, 40$^{\circ}$~90$^{\circ}$ 구간에서는 ${\sigma}_c$와 I$_{sd}$가 각각 양호한 상관관계를 보여준다. 또한 상관비(K=${\sigma}_c$/I$_s$)는 약 13정도로서 일반적으로 적용되는 비, 24와 상당한 차이가 있다. 이러한 현상은 호상편마암의 구조적 및 역학적 이방성 특성으로 인한 결과라고 판단된다. 한편 맥암류에서 K가 약 23정도로서 일반적인 비 24에 상당히 접근한다. 따라서, 이방성 구조가 뚜렷한 암석에서 상관비 24는 항상 적용할 수는 없으며 일축압축강도시험과 병행 실시하여 적용하는 것이 바람직하다.다. 한편, 감작감염후 77일과 도전감염후 7일의 시점에 있어서 sRBC에 대한 면역능에 미치는 영향은 전자의 양상과 비슷하였는데 대조에 비하여 지연형 과민반응과 로제트 형성능이 현저하게 저하되었다. 이 시점에 있어서의 유충회수율은 대조가 10.5% 이었는데 비하여 8.3%이었다.e also compared for the cases of Kim et al, which again gives promising agreement.면적 306~453$\textrm{cm}^2$, 유색계의 경우 수당면적 340~453$\textrm{cm}^2$ 일 때 경제능력을 제대로 발휘할 수 있고 경제성이 있는 것으로 나타났다. 첨가구가 높은 경향이 있었다8.4%. 79.7% 그리고 80.2%였다.

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Gene Expression Profile of Rat Hypothalamus Treated with Electroacupuncture at ST36 Acupoint (족삼리 전침자극에 의한 흰쥐 hypothalamus의 유전자 발현 profile 분석)

  • Rho Sam Woong;Lee Gi Seog;Choi Gi Soon;Na Young In;Hong Moo Chang;Shin Min Kyu;Min Byung il;Bae Hyun Su
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.18 no.4
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    • pp.1041-1054
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    • 2004
  • Electroacupuncture (EA) has been reported to increase pain threshold, and to enhance the NK cell activity by up-regulation of IFN-γ and endogenous β-endolphin. For the purpose of understanding the molecular mechanism of EA stimulation, we analyzed the gene expression profile of rat hypothalamus, treated on Zusanli (ST36) with EA, in comparison with control group by oligonucleotide chip microarray (Affymetrix GeneChip Rat Neurobiology U34 Array) and real-time RT-PCR. Sprague-Dawley (S-D) male rats were stimulated at the Zusanli (ST36) acupoint in restriction holder. Simultaneously the control group was given only holder stress without EA stimulation. In order to prove the appropriateness of EA treatment, we measured spleen NK cell activity with standard 51Cr release assay. NK cell activity of EA group was significantly increased comparing to control group. The microarray and PCR results show that EA treatment up-regulates expression of genes associated with 1) nerve growth such as NGF induced factor A and VGF, 2) signal transduction such as 5HT3 receptor subunit, AMPA receptor binding protein and Na-dependent neurotransmitter transporter, and 3) anti-oxidation such as superoxide dismutase and glutathione S-transferase. In addition, the activity of the anti-oxidative enzyme, SOD of hypothalamus, liver and RBC was enhanced compared to that of control. The list of differentially expressed genes may implicate further insight on the mechanism of acupuncture effects.

Hematological and Serum Chemical Values in Pere David′s Deer and Wapiti (사불상 및 와피티 사슴의 혈액 및 혈청화학치)

  • Shin Nam-Sik;Kwon Soo-Wahn;Han Duk-Hwan;Lee Heung-Shik
    • Journal of Veterinary Clinics
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    • v.11 no.1
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    • pp.471-478
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    • 1994
  • The hematologic values and serum chemistry were examined healthy male 12 Pere David's Deer and 8 Wapiti at the Farmland Zoological Garden. In wapiti, the blood values collected from horn and jugular vein were compared. The results were as follows: 1. There were not any significant differences in hematologic values and serum chemistry of blood col. looted from horn and jugular vein in wapiti. 2. In Pere David's Deer, Mean$\pm$SD Values of White Blood Cell(WBC) count were 4673.50$\pm$1893.42/${mu}ell$, of Neutrophils 65.42$\pm$12.7%, of Lymphocytes 31.50$\pm$8.68%, of Monocytes 0.25$\pm$0.43%, of Eosinophils 0.17$\pm$0.37%. of Basophils, of Red Blood Cell(RBC) 9.93$\pm$1.38$\times$10/${mu}ell$, of PCV 54.25$\pm$6.25%, of Hb 16.89$\pm$2.43g/㎗, of Tot protein 7.63$\pm$0.6g/㎗, of Cholesterol 87.25$\pm$10.76mg/㎗, of Glucose 109.36$\pm$43.7mg/㎗, of SGOT 85.17$\pm$33IU/$\ell$, of SGPT 102.92$\pm$31.49IU/$\ell$, of BUN 21.00$\pm$5.18mg/㎗, of Creatinine 2.1$\pm$0.43mg/㎗, of ALKP 156$\pm$32.72IU/$\ell$, of Ca 11.28$\pm$1.04mg/㎗, of Na 127.92$\pm$10.04mmo1/$\ell$, of NH3 102.83$\pm$42.25mmo1/$\ell$, of Cl 105.91$\pm$3.45mmo1/$\ell$, respectively. 3. In wapiti, Mean$\pm$SD Values of White Blood Cell(WBC) count were 3450.00$\pm$1040.73/${mu}ell$, of Neutrophils 53.88$\pm$7.42%, of Lymphocytes 36.00$\pm$5.72%, of Monocytes 1.13$\pm$1.05%, of Eosinophils 1.38$\pm$1.93%, of Basophils 7.63$\pm$4.15%, of Red Blood Cell 10.09$\pm$1.3$\times$10/${mu}ell$, of PCV 46.88$\pm$3.33%, of H 15.09$\pm$1.48g/㎗, of Total protein 7.15$\pm$0.73g/㎗, of Cholesterol 78.63$\pm$12.27mg/㎗, of Glucose 75.63$\pm$33.22mg/㎗ , of SGOT 79.25$\pm$18.43IU/$\ell$, of SGPT 36.50$\pm$19.20IU/$\ell$, of BUN 29.63$\pm$4.15mg/㎗, of Creatinine 1.35$\pm$0.21mg/㎗ , of ALKP 283.50$\pm$88.13IU/$\ell$, of Ca 9.78$\pm$0.43mg/㎗ , of Na 120.00$\pm$10.02mmol/$\ell$, of NH3 261.50$\pm$ 103.46mmol/$\ell$, of Cl 92.00$\pm$9.19mmol/$\ell$, respectively.

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