Browse > Article
http://dx.doi.org/10.5187/JAST.2006.48.4.503

Effects of Dietary Lactobacillus brevis Supplementation on Growth Performance, Dry Matter and Nitrogen Digestibilities, Blood Cell Counts and Fecal Odor Emission Compounds in Growing Pigs  

진영걸 (Department of Animal Resource & Science, Dankook University)
민병준 (Department of Animal Resource & Science, Dankook University)
조진호 (Department of Animal Resource & Science, Dankook University)
김해진 (Department of Animal Resource & Science, Dankook University)
유종상 (Department of Animal Resource & Science, Dankook University)
김인호 (Department of Animal Resource & Science, Dankook University)
Publication Information
Journal of Animal Science and Technology / v.48, no.4, 2006 , pp. 503-512 More about this Journal
Abstract
This study was conducted to investigate the effects of dietary Lactobacillus brevis (3.4×108 CFU/g) supplementation on growth performance, DM and N digestibilities, blood cell counts and fecal odor emission compounds in growing pigs. Ninety six crossbred [(Landrace×Yorkshire)×Duroc] pigs with an initial BW of 24.60±1.28kg were used for 42-d feeding trial according to a completely randomized design. Three corn- soybean meal based dietary treatments included: 1) CON (basal diet); 2) LB1 (basal diet + Lactobacillus brevis 0.2%) and 3) LB2 (basal diet+Lactobacillus brevis 0.4%). There were three dietary treatments with eight replicate pens per treatment and four pigs per pen. Through the entire experimental period, ADG, ADFI and gain/feed had no significant differences among treatments(P>0.05). Nitrogen digestibility was increased in LB1 and LB2 treatments compared to CON treatment (linear effect, P<0.05), however, DM digestibility had no significant difference among all the treatments (P>0.05). The WBC, RBC and lymphocyte concentrations in whole blood were not affected by treatments (P>0.05). Fecal NH3N and H2S concentrations were significant decreased in LB2 treatment compared to CON treatment (linear effect, P<0.05). Fecal VFA (acetic acid and propionic acid) concentration was also reduced in LB2 treatment compared to CON treatment (linear effect, P<0.05). In conclusion, Lactobacillus brevis (3.4×108 CFU/g) supplementation at the level of 0.4% can improve nitrogen digestibility and decrease the concentrations of fecal odor emission compounds in growing pigs.
Keywords
Lactobacillus brevis;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Aattouri, N., Bouras, M., Tome, D., Marcos, A. and Lemonnier, D. 2002. Oral ingestion of lactic acid bacteria by rats increases lymphocytic proliferation and interferon. production. Br. J. Nutr. 87:367-373   DOI
2 Chaney, A. L. and Marbach, E. P. 1962. Modified regents for determination of urea and ammonia. Clin. Chem. 8: 131
3 Hong. J. W.. Kim. I. H.. Kwon, O. S .. Kim. J. H.. Min. B. J. and Lee. W. B. 2002. Effects of dietary probiotics supplementation on growth performance and fecal gas emission in nursmg and finishing pigs. J. Anim. Sci & Technol. (Kor.) 44:305-314   DOI
4 Imoto. S. and Namioka, S. 1978. VFA production in the pig large intestine. J. Anim. Sci. 47:467-478   DOI
5 Ji. F. and Kim. S. W. 2002. Reducing odor in swine production: Effect of enzymes and probiotics on ammonia production. J. Anim. Sci. Vol. 80 (Suppl. I)
6 Jasek. S. R.. Kalinowska, R.. Knecht. D. and Pawiak, R. 1992. Effect of Biogen probiotic addition on reproduction results and physiological indices in pigs. Rocz. Nauk. Zootech. 31 :239
7 Jonsson. E. and Conway, P. 1992. Probiotics for pigs. In: R. Fuller (Ed.) Probiotics: The Scientific Basis. Chapman & Hall. London. pp:260-316
8 Kadota, H. and Ishida. Y. 1972. Production of volatile sulfur compounds by microorganisms. Ann. Rev. Microbiol. 26:127-138   DOI   ScienceOn
9 Kil, D. Y.. Lim, S. J.. Tian, J. Z.. Kim, B. G.. Kim, K. S. and Kim. Y. Y. 2004. Effect of continuous feeding of probiotics on growth performance, nutrient digestibility. blood urea nitrogen and immune responses in pigs. J. Anim. Sci & Technol. (Kor.) 46:39-48   DOI
10 Kornegay. E. T., Wood. C. M.. Ball, G. G. and Risley. C. R. 1990. Use of Lactobacillus acidophilus for growing and finishing pigs. VA Polytech. Inst. State Univ. Anim. Sci. Res. Rep. 9:13
11 AOAC. 1995. Official method of analysis. 16th Edition. Association of Official Analytical Chemists. Washington. DC
12 Avery. G. L. Merva. G. E. and Gerrish. J. B. 1975. Hydrogen sulfide production in swine confinement units. Trans. ASAE. 17: 149-151
13 Baird. D. M. 1977. Probiotics help boost feed efficiency. Feedstuffs. 49:11-12
14 Heilig. H. G. H. J.. Zoetendal. F. G .. Vaughan. E. E.. Marteau. P.. Akkermans. A. D. L and de Vos. W. M. 2002. Molecular diversity of Lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16S ribosomal DNA. Appl. Environ. Microbiol. 68:114-123   DOI
15 Apgar. G. A.. Kornegay. F. T.. Lindemann. M. D. and Wood. C. M. 1993. The effect of feeding various levels of Bifidobacteriurn globosurn A on the performance. gastrointestinal measurements. and immunity of weanling pigs and on the performance and carcass measurements of growing-finishing pigs. J. Anim, Sci. 71:2173-2179
16 Collins. M. D. and Gibson. G. R. 1999. Probiotics, prebiotics and synbiotics: approaches for modulating the microbial ecology of the gut. Anim. J. Clin. Nutr. 69 (suppl):1052-1057   DOI
17 Maxwell. C. Y.. Buchanan. D. S.. Owens. F. N.. Gilliland. S. E.. Luce. W. G. and Vend. R. 1983. Effect of probiotic supplementation on performance, fecal parameters and digestibility In growing finishing swine. Oklahoma Agric. Exp. Sta.. Anim. Sci. Res. Rep. 114:157
18 Elsden. S. R.. Hitchcock. M. W. S.. Marshall. R. A. and Phillipson. A. T. 1946. Volatile acid in the digesta of ruminants and other animals. J. Exp. Biol. 22: 191-202
19 Chen. Y. J., Son, K. S., Min, B. J.. Cho, J. H., Kwon, O. S. and Kim. I. H. 2005. Effects of dietary probiotic on growth performance, nutrients digestibility, blood characteristics and fecal noxious gas content in growing pigs. Asian-Aust. J. Anim. Sci. 18:1464-1468   DOI
20 Chen. Y. J., Min, B. J., Cho, J. H., Kwon, O. S., Son. K. S.. Kim. I. H. and Kim. S. J. 2006. Effects of dietary Enterococcus faecium SF68 on growth performance. nutrients digestibility. blood characteristics and fecal noxious gas content in finishing pigs. Asian-Aust. J. Anim. Sci. 19:406-411   DOI
21 Fuller. R. 1989. Probiotics in man and animals. J. Appl. Bacteriol, 66:365-378   DOI
22 Hale. O. M. and Newton, G. L. 1979. Effects of a nonviable Lactobacillus species fermentation product on performance of pigs. J. Anim. Sci, 48:770
23 Han. I. K.. Lee, J. H .. Piao. X. S. and Li, D. F. 2001. Feeding and management system to reduce environmental pollution in swine production: A review. Asian-Aust. J. Anim. Sci. 14:432-444   DOI
24 Burgstaller. G., Ferstl, R. and Apls, H. 1984. The addition of lactic acid bacteria (Streptococcus faecium SF-68) to a milk replacer for calf feeding. Zuchtungskunde. 56: 156-162
25 Shon, K. S., Hong, J. W., Kwon, O. S., Min, B. J., Lee, W. B., Kim, I. H., Park, Y. H. and Lee, I. S. 2005. Effects of Lactobacillus reuteri-based direct-fed microbial supplementation for growing-finishing pigs. Asian-Aust. J. Anim. Sci. 18:370-374   DOI
26 Bomba. A.. Nemcova, R.. Gancarcikova, S.. Herich. R.. Guba, P. and Mudronova, D. 2002. Improvement of the probiotic effect of micro-organisms by their combination with maltodextrins, fructo-oligosaccharides and polyunsaturated fatty acids. British Journal of Nutrition. 88 (Suppl.) 1: 95-99   DOI   ScienceOn
27 Sandine, W. E. 1979. Role of lactobacillus in the intestinal tract. J. Food Protect. 42: 259-262   DOI
28 SAS. 1996. SAS user's guide. Release 6.12 edition. SAS Institute. Inc Cary NC. USA
29 Spriet, S. M.. Decuypere. .J. A. and Henderickx. H. K. 1987. Effect of Bacillus toyoi (Toyocerin) on the gastrointestinal microflora. concentration of some bacterial metabolites. digestibility of the nutrients and the small intestinal mean retention time in pigs. Meded. Fac. Landbouwkd. Rijksuniv. Gent. 52: 1673
30 Tortuero. F.. Rioperez, J.. Fernandez, E. and Rodriguez. M. L. 1995. Response of piglets to oral administration of lactic acid bacteria. J. Food Protect. 58:1369-1374   DOI
31 Wenk, C. 2000. Recent advances in animal feed additives such as metabolic modifiers, antimicrobial agents, probiotics, enzymes and highly available minerals. Asian-Aust, J. Anim. Sci. 13:86-95   DOI
32 Zahn, J. A., Hatfield, J. L., Do, Y. S., DiSpirito, A. A.. Laird, D. A. and Pfeiffer. R. L. 1997. Characterization of volatile organic emissions and wastes from a swine production facility. J. Environ. Qual. 26: 1687 -1696   DOI
33 Hays. V. W. 1969. Use of Drugs in Animal Feeds. National Academy of Science. Washington. D. C
34 Heber. A. J.. Duggirala R. K.. Ni. J. Q.. Spence. M. L.. Haymore. B. L.. Adamchuk, V. I.. Bundy. D. S.. Sutton. A. L.. Kelly. D. T. and Keener. K. M. 1997 Manure treatment to reduce gas emissions from large swine houses. Vinkeloord, The Netherlands. pp: 449-458
35 Elina, R.. Erja, M.. Maria S.. Merja. R.. Johannes. A. and Airi. P. 2003. Probiotic and milk technological properties of Lactobacillus brevis. Int. J. of Food Microbiol. 83:63-74   DOI   ScienceOn
36 NRC. 1998. Nutrient requirement of pigs. 10th Edition. National Research Council. Academy Press. Washington, D. C
37 Otto. E. R., Yokoyama, M.. Hengernuehle, S.. von Bermuth, R. D., van Kempen, T. and Trottier. N. L. 2003. Ammonia, volatile fatty acids. phenolics, and odor offensiveness in manure from growing pigs fed diets reduced in protein concentration. J. Anim. Sci. 2003. 81:1754-1763
38 Peterson, R. G. 1985. Design and Analysis of Experiments. Marcel Dekker. New York
39 Franklin. M. A.. Mathew, A. G.. Vickers. J. R. and Clift. R. A. 2002. Characterization of microbial populations and volatile fatty acid concentrations 111 the jejunum. ileum. and cecum of pigs weaned at 17 vs 24 days of age. J. Anim. Sci, 80:2904-2910
40 Banwart. W. L. and Bremner. J. M. 1975. Formation of volatile sulfur-compounds by microbial decomposition of sulfur-containing amino acids in soils. Soil. Biol. Biochem. 7:3590-364