• Title/Summary/Keyword: rate constant(k)

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Optimizing Conditions for Streptomyces chibaensis J-59 Glucose Isomerase Production Using Response Surface Methodology (반응표면분석에 의한 방선균 Streptomyces chibaensis J-59 포도당 이성화효소의 생산 최적화)

  • Joo, Gil-Jae;Park, Heui-Dong
    • Current Research on Agriculture and Life Sciences
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    • v.14
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    • pp.101-110
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    • 1996
  • Using response surface methodology(RSM), the various conditions(agitation speed, air flow, glucose concentration) in jar fermentor culture were investigated to find the optimum conditions for maximum enzyme production. Central-composite-design was used to control the variable constant in the experiment. The glucose isomerase production of Steptomyces chibaensis J-59 was mostly affected by the air flow rate and glucose concentration. The estimated optimum conditions were as follows: 1% birchwood xylan, 1.5% CSL, 0.1% $MgSO_4{\cdot}7H_2O$, 0.012% $CoCl_2{\cdot}6H_2O$, pH 7.0; air flow, 2.2vvm; agitation speed, 587rpm; glucose concentration, 0.586%. Experimental values(7.43GIU/ml) for the enzyme production obtained from the given optimum conditions had a almost resemblane to response values(7.67GIU/ml) predicted by the RSM. The jar fermentor culture by the RSM produced xylose isomerase about 2.7 times as much as the baffled flask culture.

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Improvement of Grouting by Short-period Vibration Energy (단주기 진동에너지에 의한 그라우팅 보강효과)

  • Seo, Moonbok;Kwon, Sanghoon;Lee, Bongjik
    • Journal of the Korean GEO-environmental Society
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    • v.16 no.7
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    • pp.35-42
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    • 2015
  • Grouting method has been widely used for the ground improvement and stabilization: mostly to block or control the ground water in the early years and to improve the ground, repair the structure in recent years, ever increasing its use. Despite many advantages so far, the existing grouting method also has some shortcomings including uncertain permeation of grouting with gravity type if the voids between the soil particles are narrow, and problems due to the relaxation of the neighboring ground when injected using injection pressure. As an alternative, a vibration injection method with constant amplitude and frequency has been developed in recent years, with the vibration grouting being reported to have a permeability increasing effect of grout material compared with the positive pressure injection type. Accordingly, the purpose of this study is to investigate the improvement effect of the vibration grouting that applies short-period vibration energy by varying vibration cycle, vibration time and ground conditions to evaluate the strength enhancing effect of grouting materials, expansion effect of grouting body, ground improvement effect of the grouting and the penetration characteristics of the rock joint. The findings of this study show the improved compressive strength of grout, expansion of grouting body and increased penetration rate, according to the vibration compared with non-vibration under the loose soil condition.

Experimental Study on Reduction of Nitrogen-Containing Compounds Contained in Crude Methylnaphthalene Oil by Solvent Extraction (II) (용매 추출에 의한 조제 메틸나프탈렌유에 함유된 함질소화합물의 저감에 관한 실험적 연구(II))

  • Kang, Ho-Cheol;Kim, Su Jin
    • Applied Chemistry for Engineering
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    • v.33 no.5
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    • pp.477-481
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    • 2022
  • As a part of improving the quality of crude methylnaphthalene (CMNO), this study was experimentally examined the reduction of nitrogen-containing compounds (NC) present in the CMNO by solvent extraction. The CMNO was composed of three kinds of NC [quinolone (QU), iso-quinoline (IQU), indole (IN)], three kinds of bicyclic aromatic compound [BAC; naphthalene (NA), 1-methylnaphthalene (1MNA), 2-methylnaphthalene (2MNA)] and biphenyl (BP) etc., in addition to an aqueous formamide solution, which were used as raw materials and a solvent, respectively. The increase in the volume fraction of water to the solvent in the initial state (yw,0) caused a sharp decrease in the distribution coefficient and the yield of NC, but conversely raised the increased selectivity of NC based on 2MNA. The compositions of QU, IQU and IN in the raffinate oil recovered through the equilibrium extraction of batch co-current 5-stage under constant conditions [yw,0 = 0.1, volume fraction of solvent to feed (CMNO) at the initial state = 1, operating temperature = 303 K, liquid-liquid contacting time = 72 h] were reduced by about 51.5%, 55.2%, and 71.8%, respectively, when compared to those of CMNO. From the excellent reduction rate of NC, the formamide extraction method suggested in this study can be expected to be a useful reduction method for NC contained in the CMNO.

Effect of Water on the Lightweight Air-Mixed Soil Containing Silt Used for Road Embankment (도로성토체로 사용된 실트질 계열의 경랑기포혼합토에 대한 물의 영향)

  • Hwang, Joong-Ho;Ahn, Young-Kyun;Kim, Tae-Hyung
    • Journal of the Korean Geotechnical Society
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    • v.26 no.2
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    • pp.23-32
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    • 2010
  • This study was especially conducted to find out the characteristics of the lightweight air-mixed soil (slurry density 10 kN/$m^3$) containing silt related to water. Compression strength, permeability, and capillary height of the lightweight air-mixed soil were studied, and also to support these studies, the structure of that soil was analyzed in detail. Air bubbles of various sizes are inside the lightweight air-mixed soil, and its distribution in a location is almost constant. A numerous tiny pores are inside the air bubbles so that the lightweight air-mixed soil can be saturated with water. Porosity is also estimated through the image analysis. Peak strength of the lightweight air-mixed soil is not dependent on water, but behavior of stress-strain is affected by the water. Permeability is about $4.857{\times}10^{-6}cm/sec$, which is a little bit higher than the clay's permeability. Capillary rise occurs rapidly at the beginning of the test until the lapse of 100 minutes and then its increase rate becomes slow. The capillary rise causes the increase of the density of the lightweight air-mixed soil, and thus it is required to pay attention to this phenomenon during structure design and maintenance of the lightweight air-mixed soil.

A Comparison of Discriminating Powers Between 14 Microsatellite markers and 60 SNP Markers Applicable to the Cattle Identification Test (소 동일성 검사에 적용 가능한 14 Microsatellite marker와 60 Single Nucleotide Polymorphism marker 간의 판별 효율성 비교)

  • Lim, Hyun-Tae;Seo, Bo-Yeong;Jung, Eun-Ji;Yoo, Chae-Kyoung;Yoon, Du-Hak;Jeon, Jin-Tae
    • Journal of Animal Science and Technology
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    • v.51 no.5
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    • pp.353-360
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    • 2009
  • When 14 microsatellite (MS) markers were applied in the identifying test for 480 Hanwoo, the discriminating power was estimated as $3.43{\times}10^{-27}$ based on the assumption of a random mating group (PI). This rate is 1,000 times higher than that of 60 single nucleotide polymorphism (SNP) markers. On the other hand, the power of the 60 SNP markers was estimated as $4.69{\times}10^{-20}$ and $8.02{\times}10^{-12}$ on the assumption of a half-sib mating group ($PI_{half-sibs}$) and a full-sib mating group ($PI_{sibs}$), respectively. These powers were 10 times and 10,000 times higher than those of the 14 MS markers. The results indicated that the total number of alleles (MS vs SNP = 146 vs 120) acted as a key factor for the discriminating power in a random mating population, and the total number of markers (MS vs SNP = 14 vs 60) was a dominant influence on the power in half-sib and full-sib populations. In the Hanwoo population, in which it was assumed that the entire population is the enormous half-sib group formed by the absolute genetic contribution of a few nuclear bulls, there will be only a 10 times difference in the discriminating power between the 14 MS markers and the 60 SNP makers. However, the probability of not excluding a candidate parent pair from the parentage of an arbitrary offspring, given that only the genotype of the offspring ($PNE_{pp}$) was 1,000 times higher as shown by the 14 MS markers than that by the 60 SNP markers. The strong points of SNP makers are the stability of the variation (low mutation rate) and automation of high-throughput genotyping. In order to apply these merits for the practical and constant Hanwoo identity test, research and development are required to set a cost-effective platform and produce a homemade apparatus for SNP genotyping.

STUDY ON MAXILLOFACIAL GROWTH AND PHYSICAL DEVELOPMENT OF GROWING CHILDREN (성장기 아동의 악안면 성장과 신체발육에 관한 연구)

  • Hwang, Chung-Ju;Kyung, Seung-Hyun
    • The korean journal of orthodontics
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    • v.27 no.6 s.65
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    • pp.963-978
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    • 1997
  • Orthodontic patients are individuals that grow and develop ;therefore selection of the proper time for orthodontic treatment Is considered to be one of the most difficult and yet difficult factor.Since the development of cephalometric X-ray, amount and pattern of craniofacial growth change with aging could be predicted and became useful in the process of orthodontic treatment. The relationship between the mean values of cephalometric measurements and body height and weight was studied among the groups(boys and girls) of Korean children from the ages of 3-years to 12-years. 126 boys and 90 girls with no abnormality in growth and development and no history of orthodontic treatment from the ages of 3 years to 12 years were chosen as subjects: Cephalometric X-ray were taken for 2 years and hard tissue analysis based on Burstone's COGS, which was divided into measurements of 6 parts (Cranial base, Maxillar and Mandible, Vertical measurements, Horizontal measurements, Basal bone relationship, Dental measurements). The relationship between craniofacial growth and body height & weight was studied. The following conclusions were obtained : 1. The maximum growth in the measurements of cranial base, N-Ar(FH), N-Ba(FH) corresponded with the age with the maximum increasein body height & weight in both boys and girls. 2. Genial angle gradually decreased with aging in both boys and girls. 3. N-ANS(L) showed greater amount of growth than ANS-Me(L), and this had greater influence on facial profile. 4. $N-A-Pog^{\circ}$ decreased with aging, and mandibular growth exceeded maxillary growth in amount and rate. 5. Length of Y-axis increased, but Y-axis to FH plane remained constant. This show that mandible grows at a constant angulation to cranial base. 6. As Permanent teeth erupt, interincisal angle deceased.

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Identification of Active Agents for Reductive Dechlorination Reactions in Cement/Fe (II) Systems by Using Cement Components (시멘트 구성성분을 이용한 시멘트/Fe(II)의 TCE 환원성 탈염소화 반응의 유효반응 성분 규명)

  • Jeong, Yu-Yeon;Kim, Hong-Seok;Hwang, In-Seong
    • Journal of Soil and Groundwater Environment
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    • v.13 no.1
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    • pp.92-100
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    • 2008
  • Experimental studies were conducted to identify the active agents for reductive dechlorination of TCE in cement/Fe(II) systems focusing on cement components such as CaO, $Fe_2O_3$, and $Al_2O_3$. A hematite that was used to simulate an $Fe_2O_3$ component in cement was found to have degradation efficiencies (k = 0.641 $day^{-1}$) equivalent to that of cement/Fe(II) systems in the presence of CaO/Fe(II), only when it contained an aluminum impurity$(Al_2O_3)$. When the effect of $Al_2O_3$ content of hematite/CaO/$Al_2O_3$/Fe(II) system was tested, the mole ratio of $Al_2O_3$ to CaO affected the rate of TCE degradation with an optimum ratio around 1 : 10 that resulted in a rate constant of 0.895 $day^{-1}$. In the SEM images of hematite/CaO/$Al_2O_3$/Fe(II) systems, acicular crystals were also found that were also observed in cement/Fe(II) systems. Thus it was suspected that these crystals were reactive reductants and that they might be goethite or ettringite that are known to have acicular structures. An EDS element map analysis revealed that these crystals were not goethite crystals. A subsequent experiment that tested reactivities of compounds formed during the ettringite synthesis showed that ettringite and minerals associated with ettringite formation are not reactive reductants. These observations conclude that a mineral containing CaO and $Al_2O_3$ with a acicular structure could be a major reactive reductant of cement/Fe(II) systems.

Smile Rearrangement of Herbicidal Flazasulfuron (제초성 Flazasulfuron의 Smile 자리옮김 반응)

  • Lee, Gwnag-Jae;Kim, Yong-Jip;Kim, Dae-Whang;Sung, Nack-Do
    • Applied Biological Chemistry
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    • v.39 no.1
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    • pp.70-76
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    • 1996
  • A series of the herbicidal pyridylsulfonyl areas, none substitutent, 1-(4,6-dimethoxypyrimidine-2-yl)-3-(2-pyridylsulfonyl) urea, 3 and 3-trifluoromethyl substitutent, 1-(4,6-dimethoxypyrimidine-2-yl)-3-(3-trifluoromethyl-2-pyridylsulfonyl) urea, 5(Flazasulfuron) were synthesizied and the rate of hydrolysis of their has been studied in 25%(v/v) aqueous dioxane at $45^{\circ}C$. From the results of solvent effect($m{\ll}1,\;n{\ll}3\;&\;{\mid}m{\mid}{\ll}{\mid}{\ell}{\mid}$), thermodynamic parameter (${\Delta}S^{\neq}=0.54{\sim}\;-2.19\;e.u.\;&\;{\Delta}H^{\neq}=0.025\;Kcal.mol.^{-1}$), hydrolysis product analysis, $pK_a$ constant(3: 4.9 & 5: lit.4.6) and the rate equation, a marked difference in the kinetics of the reaction of 3 and 5(Flazasulfuron) was observed. It may be concluded that the hydrolysis of 5 proceeds through the $A-S_N2Ar$ reaction via conjugate acid$(5H^+)$ below pH 7.0, whereas, above pH 9.0, the hydrolysis proceeds through irreversibly $(E_1)_{anion}$ and reversibly $(E_1CB)_R$ mechanism via conjugate base(CB), respectively. But in case of 5, $A-S_N2Ar,\;(E_1)anion\;and\;(E_1CB)_R$ mechanism involved Smile rearrangement. The mate of rearrangement of 5 to a 3-trifluoromethyl-2-pyridylpyrimidinyl urea(PPU) in acid and 3-trifluoromethyl-2-pyridyl-4.6-dimethoxypyridinyl amine (PPA) in base was increased about 3.5 times by the introduction of trifluoromethyl group in the 3-position on the 2-pyridyl ring. From the basis of these findings, a possible mechanism for the hydrolysis of 5 was proposed and discussed.

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A Study on Recovery from Potentially Lethal Damage Induced by $\gamma-Irradiation$ in Plateau-phase Vero Cells in vitro (평형기의 Vero세포계에서 방사선($\gamma$-선) 조사 후 발생한 잠재치사 손상의 회복에 관한 연구)

  • Kim, Il-Han;Choi, Eun-Kyung;Ha, Sung-Whan;Park, Charn-Il;Cha, Chang-Yong
    • Radiation Oncology Journal
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    • v.6 no.1
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    • pp.1-11
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    • 1988
  • Recovery from potentially lethal damage (PLDR) after irradiation was studied in plateau-phase culture of Vero cells in vitro. Unfed plateau-phase cells were irradiated with dose of 1 to 9Gy using Cs-137 irradiator. Cells then were incubated again and left in situ for 0, 1, 2, 3, 4, 5, 6, and 24 hours and then were trypsinized explanted, and subcultured in fresh RPMI-1640 media containing $0.33\%$ agar. Cell survival was measured by colony forming ability. An adequate number of heavily irradiated Vero cells were added as feeder cells to make the total cell number constant in every culture dish. As the postirradiation in situ incubation time increased, surviving fraction increased by PLDR. The rate of PLDR was so rapid that increased surviving fraction reached saturation level at 2 to 4 hours after in situ incubation. As the radiation dose increased, the rate of PLDR fastened and the magnitude of increased surviving fraction at saturation level by PLOR also increased. In analysis of cell survival curve fitted to the linear-quadratic model, the linear inactivation coefficient $(\alpha)$ decreased largely and reached nearly to zero but the quadratic inactivation coefficient $(\beta)$ increased minimally by increment of postirradiation in situ incubation time. So PLDR mainly affected the damage expressed as $\alpha$, In the multitarget model, significant change was not obtained in $D_0\;but\;in D_q$. Therefore, shoulder region in cell survival curve was mainly affected by PLDR and terminal slope was not influenced at all. And dose-modifying factor by PLDR was relatively higher in shoulder region, that is, in low dose area below 3 Gy.

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Population Phenology and an Early Season Adult Emergence model of Pumpkin Fruit Fly, Bactrocera depressa (Diptera: Tephritidae) (호박과실파리 발생생태 및 계절초기 성충우화시기 예찰 모형)

  • Kang, Taek-Jun;Jeon, Heung-Yong;Kim, Hyeong-Hwan;Yang, Chang-Yeol;Kim, Dong-Soon
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.10 no.4
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    • pp.158-166
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    • 2008
  • The pumpkin fruit fly, Bactrocera depressa (Tephritidae: Diptera), is one of the most important pests in Cucurbitaceae plants. This study was conducted to investigate the basic ecology of B. depressa, and to develop a forecasting model for predicting the time of adult emergence in early season. In green pumpkin producing farms, the oviposition punctures caused by the oviposition of B. depressa occurred first between mid- and late July, peaked in late August, and then decreased in mid-September followed by disappearance of the symptoms in late September, during which oviposition activity of B. depressa is considered active. In full-ripened pumpkin producing farms, damaged fruits abruptly increased from early Auguest, because the decay of pumpkins caused by larval development began from that time. B. depressa produced a mean oviposition puncture of 2.2 per fruit and total 28.8-29.8 eggs per fruit. Adult emergence from overwintering pupae, which was monitored using a ground emergence trap, was first observed between mid- and late May, and peaked during late May to early June. The development times from overwintering pupae to adult emergence decreased with increasing temperature: 59.0 days at $15^{\circ}C$, 39.3 days at $20^{\circ}C$, 25.8 days at$25^{\circ}C$ and 21.4 days at $30^{\circ}C$. The pupae did not develop to adult at $35^{\circ}C$. The lower developmental threshold temperature was calculated as $6.8^{\circ}C$ by linear regression. The thermal constant was 482.3 degree-days. The non-linear model of Gaussian equation well explained the relationship between the development rate and temperature. The Weibull function provided a good fit for the distribution of development times of overwintering pupae. The predicted date of 50% adult emergence by a degree-day model showed one day deviation from the observed actual date. Also, the output estimated by rate summation model, which was consisted of the developmental model and the Weibull function, well pursued the actual pattern of cumulative frequency curve of B. depressa adult emergence. Consequently, it is expected that the present results could be used to establish the management strategy of B. depressa.