• 한국가축번식학회지
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• 제25권1호
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• pp.43-49
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• 2001

본 연구는 Cu중독이 rat의 생리현상에 미치는 영향을 구명하고자 연속적인 Cu투여가 정소의 중량, 정자수, 활력, 장기중량 및 조직학적 변화를 조사하였다. 1. 황산Cu 1,000, 2,000 및 4,000 ppm/kg을 rat에 투여했을 때 정소중량은 정상대조군에 비해 점차 감소하였으나 유의한 변화는 인정되지 않았다. 2. 황산Cu 1,000, 2,000 및 4,000 ppm/kg을 rat에 투여했을 때 정자수는 정상대조군에 비해 점차 감소하였으나 유의한 변화는 인정되지 않았다. 3. 황산Cu 1,000, 2,000 및 4,000 ppm/kg을 rat에 투여했을 때 정자의 활력은 정상대조군에 비해 점차 감소하였고 용량이 증가할수록 정자의 활력은 유의한 감소를 나타냈다. 4. 황산Cu 1,000, 2,000 및 4,000 ppm/kg을 rat에 투여했을 때 간 및 신장의 중량은 정상대조군에 비해 점차 증가하거나 감소하였다. 5. 조직학적 관찰소견은 간세포의 중심정맥 주위성 괴사와 간문맥관 간질에 미세한 과립을 함유한 대식세포의 침윤이 관찰되었고, 신장에서는 사구체낭 및 세뇨관내 균질한 초자물질의 충만이 관찰되었다.

• 한국가축번식학회지
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• 제25권2호
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• pp.147-153
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• 2001

본 연구는 Pb중독이 rat의 생리현상에 미치는 영향을 구명 하고자 연속적인 Pb acetate 투여가 정소의 중량, 정자수, 활력, 장기중량 및 조직학적 변화를 조사하였다. 1. Pb acetate 1,000, 2,000 및 4,000 ppm/kg을 rat에 투여했을 때 정소중량은 정상대조군에 비해 감소하는 경향을 나타냈고 용량이 증가할수록 정소중량은 큰 감소경향을 나타냈다. 2. Pb acetate 1,000, 2,000 및 4,000 ppm/kg을 rat에 투여했을 때 정자수는 정상대조군에 비해 감소하는 경향을 나타냈고 용량이 증가할수록 정자수는 유의한 감소경향을 나타냈다. 3. Pb acetate 1,000, 2,000 및 4,000 ppm/kg을 rat에 투여했을 때 정자의 활력은 정상대조군에 비해 점차 감소하는 경향을 나타냈고 용량이 증가할수록 정자활력은 큰 감소경향을 나타냈다. 4. Pb acetate 1,000, 2,000 및 4,000 ppm/kg을 rat에 투여했을 때 간 및 신장의 중량은 정상대조군에 비해 점차 증감의 경향을 나타냈으며, 투여용량별 간 및 신장의 중량은 유의한 변화를 나타내지 않았다. 5. Pb acetate를 연속적으로 투여했을 때 간의 조직학적 관찰소견은 간세포 중심정맥의 주위성괴사, 호중구의 침윤, 담즙정체 및 간문맥관과 간질 대식세포의 침윤이 관찰되었고, 신장에서는 원위세뇨관 상피의 핵내 함입물과 사구체낭 및 세뇨관내 균질한 초자물질이 충만되어 있었고 림프절에서는 골수의 조혈현상이 관찰되었다.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1996년도 춘계학술대회
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• pp.191-191
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• 1996

BQ-123, a selective $ET_{A}$ receptor antagonist, reverses various responses induced by Endothelin-1 and it has been reported that BQ-123 ameliorates the cerebrovascular constriction, hypertension, and decrease of blood flow. Previously, we announced that the level of Endothelin-2 increase in the brain and spinal cord of EAE-induced lewis rat and showed the origin of ET-1 is activated macrophages. Intracisternal injection of $ET_{A}$ receptor antagonist, BQ-123(10nmol) was done for visualizing the role of endothelin-1 on the pathogenesis of EAE. BQ-123 apparently blocked the severity of clinical score of EAE and decreased the histologically observed inflammatory region. The blocking effect on the progression of EAE model following BQ-123, suggests that BQ-123 is a physiological antagonist in terms of development of the sign of multiple sclerosis.

• 대한한의학회지
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• 제23권4호
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• pp.32-44
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• 2002

Objective: In this study, we investigated the effects of Rhizoma Cimicifugae (RC) on the induction of nitric oxide (NO) in RAW 264.7 murine macrophages treated with lipopolysaccharide (LPS). Methods: We examined whether RC have an antipyretic effect by inhibition of COX and/or NOS in fever that is evoked by an intraperitoneal injection of LPS in rats. Results: 1. RC inhibited NO metabolites produced by activated murine macrophage in a dose-dependent manner. 2. RC had an antipyretic effect in a dose-dependent manner in LPS-induced fever. 3. RC inhibited levels of both COX-2 protein and iNOS protein increased by the treatment of LPS in brain tissues as well as brain blood vessels. 4. There were no changes in proteins of nNOS/COX-1. Conclusions: RC has an antipyretic effect by attenuating the level of iNOS and COX-2 protein.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1998년도 Proceedings of UNESCO-internetwork Cooperative Regional Seminar and Workshop on Bioassay Guided Isolation of Bioactive Substances from Natural Products and Microbial Products
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• pp.195-195
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• 1998

Ginkgetin was previously reported as an inhibitor of group II phospholipase A$_2$. It also inhibited in vitro arachidonate release from the activated macrophages and lymphocyte proliferation. These previous studies suggested an anti-inflammatory nature of ginkgetin, especially on chronic inflammation. In fact, ginkgetin showed potent anti-inflammatory activity against rat adjuvant-induced arthritis, a chronic inflammatory animal model, with comparable analgesic activity. In order to investigate the action mechanisms, tumor necrosis factor and interferone release were studied from human PMMC. It was found that ginkgetin clearly inhibited release of these cytoknes from human PMMC. Ginkgetin was also found to inhibit immunoglobulin M production at 1 - 10 uM. These results may contribute to antiarthritic activity of ginkgetin in vivo.

• 한국수의병리학회:학술대회논문집
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• 한국수의병리학회 2005년도 Proceedings of The 2nd Asian Society of Veterinary Pathology Symposium(Vol.2) and 2005 Annual Meeting of The Korean Society of Veterinary Pathology(Vol.9)
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• pp.94-95
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• 2005

• 사상체질의학회지
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• 제10권2호
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• pp.589-613
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• 1998

The purpose of this research was to investigate effects of Bangpoongtongsungsan water extract(BTSE) on the immune reaction, anti-allergy action and anti-inflammatory action in BALB/c mice. The administration of BTSE (500mg/kg) enhanced the cell viability of thymocytes and the population of helper T cells in splenic T-lymphocytes. BTSE suppressed the production of nitric oxide, but enhanced the phagocytic activity in peritoneal macrophages. BTSE enhanced hemagglutination titer in mice. BTSE inhibited passive cutaneous anaphylaxis induced by egg albumin in rat, the lethal anaphylaxis induced by platelet activating factor and compound 48/80 in mice, and then inhibited the degranulation of peritoneal mast cells induced by compound 48/80. BTSE did not inhibit Arthus reaction, but inhibited the delayed type hypersensitivity induced by SRBC and contact dermatitis induced by DNFB. BTSE inhibited the acute hind paw edema induced by histamine after 30 minutes, the permeability of evans blue into peritoneal cavity induced by acetic acid and the writhing syndrome induced by acetic acid. These results suggest that BTSE has an immunopotentiative action, anti-allergy action and anti-inflammatory action via the inhibition of histamine release.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.359.2-359.2
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• 2002

In the previous study, we reported that 2'-hydroxy-4'-methxoychalcone, synthetic chalcone inhibited PGE2 production in TPA- stimulated rat peritoneal macrophages by inhibiting the induction of COX-2 protein. The present study was carried out to clarify whether 2'-hydroxy-4'-methoxychalcone inhibit angiogenesis by the experimental methods in vitro and in vivo. 2'-Hydroxy-4'-methoxychalcone decreased angiogenesis of both chick embryos in the chorioallantoic membrane assay and basic fibroblast growth factor-induced vessel formation inthe mouse Martigel plug assay. (omitted)

• International Journal of Industrial Entomology and Biomaterials
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• 제45권2호
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• pp.78-83
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• 2022

There are several subspecies of Bombyx mori, whose silk sericin variants differ. Silk sericin can induce bone morphogenic protein-2 (BMP-2) in macrophages, and silk sericin from different species may have different levels of BMP-2 induction ability. In this study, silk sericin from three B. mori subspecies (Baegokjam, Yeonnokjam, and Goldensilk) was prepared. They were administered to RAW264.7 cells and BMP-2 expression level was studied. Bone regeneration was evaluated using a rat calvarial defect model. BMP-2 expression level was the highest in the Baegokjam group. The bone volume in the Baegokjam group was significantly higher than that in the Yeonnokjam group (P = 0.003). In conclusion, sericin from Baegokjam showed higher levels of BMP-2 expression and bone regeneration than those from Yeonnokjam and Goldensilk.

• 한국수의병리학회지
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• 제1권1호
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• pp.26-32
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• 1997

Protein kinase C an enzyme of signal transduction has been known to regulate cell proliferation activation as well as apoptosis in some cancer cell lines. To explore the role of PKC in the course of cell mediated autoimmune disease such as experimental autoimmune encephalomyelitis (EAE) EAE was induced in Lewis rats(6-8 weeks old) with immunization of myelin basic protein supplemented with complete Freund's adjuvants and affected spinal cords were sampled at days 13 postimmunization(PI) as peak stage of EAE and at days 21 PI as recovery stage. The spinal cords with EAE were subjected to Northern blot analysis and insitu hybridization of PKC delta which is one of prominant isotypes of PKC in the haematopoietic cells. Northern blot analysis showed that levels of PKS delta mRNA in the spinal cords of rats withEAE was significantly increased at days 13 PI in which inflammatory cells including T cells and macrophages in the EAE lesions appeared. however the stage. By in situ hybridization signals of PKC delta in EAE lesions was intensely expressed on the delta is also expressed on some brain cells in normal rat central nervous system This finding suggests that PKC plays an important role on either activation of inflammatory cells including encephalitogenic T cells and macrophages or apoptotic elimination of some inflammatory cells depending on the stge of EAE.