• Journal of Plant Biotechnology
• /
• v.39 no.3
• /
• pp.163-168
• /
• 2012

An optimization in vitro seed germination was established by using triphenol tetrazolium chloride (TTC) test, which has been known as two rare orchids (Gastrodia verrucosa Blume and Hetaeria sikokiana Tuyama) in Jeju island. We have established proper NaOCl treatment for in vitro germination of G. verrucosa and H. sikokiana through TTC test. In the case of H. sikokiana, seed viability through TTC test was high with 95% in control. However, NaOCl 1% treatment for 30 minutes showed the highest embryo swelling rate to seed viability. Likewise, swelling formation of embryos, diameter of embryos, protocorm formation and diameter of protocorms of G. verrucosa was 87%, $59{\mu}m$, 91% and $138{\mu}m$ through NaOCl 1% treatment for 30 minutes. This result will be applied on the basic information for improving in vitro seed germination rate of G. verrucosa and H. sikokiana.

• Journal of Wetlands Research
• /
• v.16 no.4
• /
• pp.335-352
• /
• 2014

In this study, we investigated and analysed the water quality, distribution of the vegetation and the wildlife to seven wetlands in Donghae city. As a result, most of the wetlands was found to be very poor water quality and some specific pollutants from entering the wetland. A total of 234 taxa on vegetation were identified including 207 species, 24 varieties, 3 formaes, 168 genera and 69 families. 2 species were recorded as designated rare plant of Korea Forest Service. The naturalized plants were 27 species. The urbanization rate of naturalization index was 8.4% and 11.6% respectively. The number of benthic macroinvertebrate taxa was 35 species, 26 families, 12 orders, 5 classes and 3 phylums. Rare species were recorded 3 species. Taxa for fishes were 5 family and 5 species. Endangered wildlife was found 1 species. Most wetlands in Donghae city were the relatively small in size, and some wetlands were separately located from forest and river ecosystems. It was limited inflow and outflow of species into a wetland from the outside. However, various vegetation were found at whole wetlands and some special species also inhabited. The wetlands performed the role as a habitat of wild life.

• Korean Journal of Plant Taxonomy
• /
• v.35 no.4
• /
• pp.337-364
• /
• 2005

The vascular flora of Mt. Deokhang (a.s.l. 1072.5m, $37^{\circ}17^{\prime}{\sim}37^{\circ}20^{\prime}N$, $128^{\circ}09^{\prime}{\sim}129^{\circ}03^{\prime}E$), one of the limestone areas in Korea and is located between Samcheok-si and Taebaek-si, Gangwon-do, is surveyed from 10 Oct. 2003 to 4 Oct. 2005. The characteristics of the flora from the phytogeographical point of view is also discussed. The number of the vascular plant taxa identified in this study is 590 in total which belongs to 90 families, 337 genera, 537 species, 4 subspecies, 46 varieties and 3 forms. Of them, 30 taxa are endemic to Korea, 4 taxa are legally protected ones, and 17 taxa are generally regarded as rare and endangered species in Korea. Naturalized plants are 19 taxa. The phytogeographical importance and the conservation strategy of the flora of Mt. Deokhang focusing on either rare, endemic or phytogeographically important species are discussed.

• Journal of agriculture & life science
• /
• v.46 no.3
• /
• pp.27-36
• /
• 2012

The purpose of this study was to investigate the structure of the vegetation on the trail of Hallyeo Marine & Coastal National Park. Twenty six $10{\times}10m$ plots were established along the Jisimdo trail. The vascular plants were surveyed from March 2011 to October 2011. Camellia japonica was a major woody species in the study area, while Pinus thunbergii and Zanthoxylum ailanthoides were co-dominant tree species. There were positive correlations between Distylium racemosum and Ligustrum japonicum var. japonicum; Callicarpa japonica var. luxurians and Eurya japonical; Actinodaphne lancifolia and Cinnamomum japonicum; A. lancifolia and Machilus japonica. Positive correlations were found between A. lancifolia and P. thunbergii; Z. ailanthoides and C. japonicum. Species diversity (H') was ranged from 0.51907 to 1.0217, and was relatively low compared to those of same national parks. 10 populations of Milletia japonica which are rare and endemic species, were recorded from the list of rare and endemic species of the Ministry of Environment near by ammo dumps, this study recommend the long term habitat monitoring of the species.

• Journal of Ginseng Research
• /
• v.43 no.1
• /
• pp.116-124
• /
• 2019

Background: Ginsenosides are known as the principal pharmacological active constituents in Panax medicinal plants such as Asian ginseng, American ginseng, and Notoginseng. Some ginsenosides, especially the 20(R) isomers, are found in trace amounts in natural sources and are difficult to chemically synthesize. The present study provides an approach to produce such trace ginsenosides applying biotransformation through Escherichia coli modified with relevant genes. Methods: Seven uridine diphosphate glycosyltransferase (UGT) genes originating from Panax notoginseng, Medicago sativa, and Bacillus subtilis were synthesized or cloned and constructed into pETM6, an ePathBrick vector, which were then introduced into E. coli BL21star (DE3) separately. 20(R)-Protopanaxadiol (PPD), 20(R)-protopanaxatriol (PPT), and 20(R)-type ginsenosides were used as substrates for biotransformation with recombinant E. coli modified with those UGT genes. Results: E. coli engineered with $GT95^{syn}$ selectively transfers a glucose moiety to the C20 hydroxyl of 20(R)-PPD and 20(R)-PPT to produce 20(R)-CK and 20(R)-F1, respectively. GTK1- and GTC1-modified E. coli glycosylated the C3-OH of 20(R)-PPD to form 20(R)-Rh2. Moreover, E. coli containing $p2GT95^{syn}K1$, a recreated two-step glycosylation pathway via the ePathBrich, implemented the successive glycosylation at C20-OH and C3-OH of 20(R)-PPD and yielded 20(R)-F2 in the biotransformation broth. Conclusion: This study demonstrates that rare 20(R)-ginsenosides can be produced through E. coli engineered with UTG genes.

• Journal of Ecology and Environment
• /
• v.44 no.4
• /
• pp.275-285
• /
• 2020

Background: In Korea, Symplocos prunifolia Siebold. & Zucc. is only found on Jeju Island. Conservation of the species is difficult because little is known about its distribution and natural habitat. The lack of research and survey data on the characteristics of native vegetation and distribution of this species means that there is insufficient information to guide the management and conservation of this species and related vegetation. Therefore, this study aims to identify the distribution and vegetation associated with S. prunifolia. Results: As a result of field investigations, it was confirmed that the native S. prunifolia communities were distributed in 4 areas located on the southern side of Mt. Halla and within the evergreen broad-leaved forest zones. Furthermore, these evergreen broad-leaved forest zones are themselves located in the warm temperate zone which are distributed along the valley sides at elevations between 318 and 461 m. S. prunifolia was only found on the south side of Mt. Halla, and mainly on south-facing slopes; however, small communities were found to be growing on northwest-facing slopes. It has been confirmed that S. prunifolia trees are rare but an important constituent species in the evergreen broad-leaved forest of Jeju. The mean importance percentage of S. prunifolia community was 48.84 for Castanopsis sieboldii, 17.79 for Quercus acuta, and 12.12 for Pinus thunbergii; S. prunifolia was the ninth most important species (2.6). Conclusions: S. prunifolia can be found growing along the natural streams of Jeju, where there is little anthropogenic influence and where the streams have caused soil disturbance through natural processes of erosion and deposition of sediments. Currently, the native area of S. prunifolia is about 3300 ㎡, which contains a confirmed population of 180 individual plants. As a result of these low population sizes, it places it in the category of an extremely endangered plant in Korea. In some native sites, the canopy of evergreen broad-leaved forest formed, but the frequency and coverage of species were not high. Negative factors that contributed to the low distribution of this species were factors such as lacking in shade tolerance, low fruiting rates, small native areas, and special habitats as well as requiring adequate stream disturbance. Presently, due to changes in climate, it is unclear whether this species will see an increase in its population and habitat area or whether it will remain as an endangered species within Korea. What is clear, however, is that the preservation of the present native habitats and population is extremely important if the population is to be maintained and expanded. It is also meaningful in terms of the stable conservation of biodiversity in Korea. Therefore, based on the results of this study, it is judged that a systematic evaluation for the preservation and conservation of the habitat and vegetation management method of S. prunifolia should be conducted.

• Journal of Environmental Impact Assessment
• /
• v.26 no.5
• /
• pp.376-384
• /
• 2017

Bangladesh is a South Asian country with subtropical monsoonal climate between the intersection of the Indo-Himalayan and Indo-Chinese sub-regions, is known as biodiversity hotspot of the Asian region. The country has different types of forest like deciduous forest, evergreen forest, mixed forest, haor (wetlands) and mangrove forest. The natural beauty of the country is increased with the presence of so many rivers, longest sea beach of the world, green plants, critical hilly regions and green agricultural forest widely spread here and there. Sundarbans is the world largest mangrove forest and world natural heritage site declared by UNESCO in 1999 situated in Bangladesh and India. About 62 percent of this mangrove forest is situated in Bangladesh and there are so many plants and animals are found in this forest. To meet the increasing demand of the large population most of the natural ecosystem is now altered, deforestation rate is increased, natural habitat of the species is disturbed. Due to the imbalance of the climate and natural system many of the rare species of the world found this region is now endangered and some of the species are extinct. Directly or indirectly they are benefited from natural resources. At present time community, based ecotourism is also an important source of income for rural poor peoples. To protect the natural resources the government is now developed so many conservation acts and policy as well NGOs are also doing work for the conservation of ecosystem and biodiversity. At present transboundary pollutants and so many natural disasters also destruct the natural resources of Bangladesh.

• Korean Journal of Plant Taxonomy
• /
• v.43 no.2
• /
• pp.128-138
• /
• 2013

The conservation status of an endemic perennial herb, Hanabusaya asiatica (Nakai) Nakai (Campanulaceae) was determined by applying the IUCN risk assessment criteria from our field study and available specimen data. Also, the GIS technology was used to develop a species distribution map to calculate the extent of occurrence (EOO) and area of occupancy (AOO) for the taxon. After two years of continuous field studies, 269 mature individuals were found in four localities in 2011, while 216 mature individuals were confirmed in three localities in 2012. Based on the following data, such as EOO (2,742 $km^2$), AOO (76 $km^2$) and estimated population size of mature individuals, the taxon, which is known as 20 localities in Korean peninsula, is evaluated as the category of Endangered (EN). A major difficulty in application of IUCN criteria to Korean rare plants were the lack of essential biological information and understanding the correct knowledge of the IUCN criteria in previous Korean studies. Sound conclusions regarding the conservation status of individual species require more intensive population studies, observations, and applying IUCN assessment procedures correctly.

• Journal of Korean Society of Forest Science
• /
• v.102 no.2
• /
• pp.239-246
• /
• 2013

This study observed changes of understory vegetation to evaluate the role of forest aggregate after 15% aggregated retention harvest in mature oak forest (> 100 years) in Gyungsangbukdo Bonghwagun in 2010 and 2011. Spontaneous responses of understory vegetation cover (%), species richness, abundance of plant growth forms (herbaceous and woody plants), and overall attributes (by Ordination analysis) were estimated in aggregate area (0.15 and n=36) and clear cut area (0.85 and n=192) in experimental site and control site (1 and n=300). Based on ordination analysis, overall change of species composition in aggregated sites were relatively lower than in harvest area. Right after treatment, total cover of cutted area slightly decreased from 15.6% to 14.7%, and species richness increased from 14 species to 22 species. Cover and richness in the both of aggregate and control sites increased. In plant growth forms, 15% aggregate harvest revealed positive effects on the abundance (cover and richness) of herbaceous plants than woody group. After retention treatment, overall, edge effect likely played major component of vegetation changes in aggregate forest and in harvested area, mechanical damage from harvest operation and change of forest structure by clear cutting were critical. As pre-treatment data, which are rare in ecological studies in Korea, were critical for interpretation between patterns that may have arisen from spatial distributions in the original forest, our experimental design have higher opportunity for long term monitoring on the effect of forest aggregate and vegetation regeneration in clear cutted area.

• Korean Journal of Environment and Ecology
• /
• v.26 no.1
• /
• pp.1-10
• /
• 2012

The habitats characteristics of Polypodium vulgare L. in Ulleung-do were investigated to compile basic data for conservation and restoration. Natural habitats were located at altitudes of 410~748m with inclinations of $12{\sim}80^{\circ}$. Sixty six vascular plants were identified from 10 quadrats in 4 habitats. Dominant species among the woody plants, based on importance value, were Acer pictum subsp. mono(49.52%) in the tree (T1) layer, Sorbus amurensis(28.99%) in the subtree (T2) and Schizophragma hydrangeoides(51.99%), Ligustrum foliosum(8.82%), Fagus engleriana(7.25%) in the shrub (S) layer. Importance value for members of the herb (H) layer were as follows: Polypodium vulgare 23.23%; Maianthemum dilatatum 9.65%; Phryma leptostachya var. asiatica 9.23%; Dryopteris crassirhizoma 8.40%; Carex shimidzensis 6.75% and Dystaenia takesimana 5.42%. The importance value of the last five species were high, so they were at affinity with Polypodium vulgare in their habitats. Species diversity was 1.18, and dominance and evenness were found to be 0.11 and 0.84, respectively. The soil types were sandy loam. Average field capacity was 30.42%, and the organic matter and pH were 17.95%, and 4.70. Correlation coefficients based on environmental factors, vegetation and soil analysis were showed that positive correlations between species diversity and species richness, whereas between species diversity and dominance, coverage of Polypodium vulgare and species richness were showed negative correlations.