• 제목/요약/키워드: rapid preparation

검색결과 438건 처리시간 0.023초

교육용 데이터베이스 시스템 구축을 위한 연구 (A Study on the Construction of Educational Database System)

  • 고대곤;문교식
    • 정보교육학회논문지
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    • 제1권2호
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    • pp.35-44
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    • 1997
  • Rapid progresses on computer and Internet technology fortell the changes in learning environments in our classrooms, which requires a timely preparation for the near future. The construction and utilization of databases that can be used in education, among other things, is the crucial subject which we need to address to. This paper discusses the various aspects of the construction of educational databases and proposes a way for effectively maintaining them.

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Rh2(Opiv)4-Catalyzed Reactions of Diazo Compound Derived from Meldrum's Acid and Styrenes. Efficient Synthesis of Cyclopropanes

  • Lee, Yong-Rok;Choi, Jung-Hyun
    • Bulletin of the Korean Chemical Society
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    • 제27권4호
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    • pp.503-507
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    • 2006
  • The rhodium(II)-catalyzed reactions of diazo compound derived from Meldrum's acid with a variety of styrenes have been examined. These reactions provide a rapid route to the preparation of cyclopropanes with a variety of substituents on the benzene ring. The mechanistic pathway for the formation of these products has been also described in terms of a stepwise mechanism.

CAD/CAM 수복의 임상적 고찰 (Clinical considerations of CAD/CAM restoration)

  • 김현정
    • 대한치과의사협회지
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    • 제57권3호
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    • pp.169-174
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    • 2019
  • The rapid evolution of CAD/CAM (Computer Aided Design / Computer Aided Manufacture) led to a dramatic impact on all disciplines of dentistry especially in the fields of prosthodontics and restorative dentistry. This article is to examine the history, advantages & disadvantages and some clinical considerations of CAD/CAM restoration.

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Evaluation of black glutinous rice (Oryza sativa L) extract as a novel nuclear stain for human sperm head assessment by microscopic examination

  • Chomean, Sirinart;Sukanto, Tanawan;Piemsup, Arreya;Chaiya, Jirattikan;Saenguthai, Kolunya;Kaset, Chollanot
    • Clinical and Experimental Reproductive Medicine
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    • 제46권2호
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    • pp.60-66
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    • 2019
  • Objective: To compare black rice (Oryza sativa L) extract with three different staining methods for human sperm head assessment. Methods: Semen samples were collected from 34 volunteers. Four smears of each ejaculate were prepared for staining using the rapid Papanicolaou (PAP) stain, SpermBlue, DipQuick, and black rice extract. The percentage of defective sperm heads (mean${\pm}$standard deviation) was compared. Results: Black glutinous rice extract, a natural dye, was used instead of hematoxylin to stain the nuclei of the sperm heads. The percentage of defective sperm heads showed a significant difference between black rice extract and DipQuick (p= 0.000). In contrast, black rice extract and rapid PAP showed no statistically significant difference (p= 0.974). A strong correlation (r = 0.761) was found between the findings obtained using rapid PAP and black rice extract. In contrast, a weak correlation (r = 0.248) was obtained between DipQuick and black rice extract for the percentage of defective sperm heads. Conclusion: The results showed good agreement and a strong correlation between the rapid PAP and black rice extract stains. The advantages of black rice extract as a novel substitute for hematoxylin for nuclear staining include ease of preparation, local availability, and favorable nuclear staining properties. Further studies could also focus on comparing staining techniques in clinical samples.

지역유형별 지역방재력에 관한 실태분석 - 강원도를 중심으로 - (Status of Local Disaster Prevention by Regional Types - Focusing on Gangwon-do -)

  • 김경남;권건주;백민호
    • 한국방재학회 논문집
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    • 제10권4호
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    • pp.33-46
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    • 2010
  • 강원도내 14개 시군을 3개 지역(도농통합형 지역, 도시형 지역, 농촌해안형 지역)으로 나누고 지역에 거주하는 지역자율방재단을 표본집단으로 하여 설문조사한 결과, 도농통합형 지역과 도시형 지역이 농촌해안형 지역보다 재해위험인지능력, 피난유도능력, 자주피난 대비정도, 방재체제 정비정도, 감시경계능력, 정보전달능력 등 모든 분야에서 낮은 것으로 분석되었다. 특히 정보전달능력은 3개 지역 모두 높게 나타났으나 자주피난 대비정도는 매우 낮은 것으로 분석되었는데 이는 정보전달능력의 경우 지방정부의 예 경보시스템 증설과 신규 설치로 인하여 재난정보를 신속히 제공할 수 있는 기반이 구축되었지만 자주피난 대비 정도는 주민 스스로가 재해 상황을 정확히 파악할 수 있도록 지속적인 재난대비 교육과 홍보를 실시하고 더불어 사전에 안전한 대피장소 확보방안 등을 모색하여야 할 것이다.

예비 전역자의 노후생활인식이 노후설계준비에 미치는 영향 (The Effects of Veterans' Perception of Old age on the Preparation for Old Age Plan)

  • 양태호;임왕규
    • 한국콘텐츠학회논문지
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    • 제14권10호
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    • pp.137-150
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    • 2014
  • 본 연구는 급속한 한국의 인구 고령화로 노후설계의 필요성이 증대되고 있음에도 불구하고 사회와 다소 소외되어 있는 군인을 대상으로 노후생활인식이 노후설계준비에 미치는 영향을 분석하였다. 연구결과 인구사회학적 특성에 따른 차이는 2개 영역(경제적 여가)의 노후설계준비에서 계급, 학력, 종교, 가정구성에서 유의한 차이를 보였으나 신체적 영역에서는 재산만이 유의한 차이를 보여 군 특성이 반영된 결과를 나타냈다. 예비 전역자의 노후생활인식이 노후설계준비에 어떠한 영향을 미치는지의 연구에서는 노후생활인식을 '성공적인 노후생활의 인식'과 '노후에 예상되는 문제점 인식'으로 상반된 개념의 두 가지 요인을 적용한 결과, 이들은 2개 영역(경제적 여가)의 노후설계준비에 정(+)의 영향을 주는 요인이었으나 신체적 영역에서는 유의하지 않았다. 전직지원교육 학습활동의 조절효과는 노후생활인식 중 '예상되는 문제점 인식'에 조절효과가 나타났으나 '성공노년 인식'요인에는 나타나지 않았다.

Effect of Sample Preparation on Prediction of Fermentation Quality of Maize Silages by Near Infrared Reflectance Spectroscopy

  • Park, H.S.;Lee, J.K.;Fike, J.H.;Kim, D.A.;Ko, M.S.;Ha, Jong Kyu
    • Asian-Australasian Journal of Animal Sciences
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    • 제18권5호
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    • pp.643-648
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    • 2005
  • Near infrared reflectance spectroscopy (NIRS) has become increasingly used as a rapid, accurate method of evaluating some chemical constituents in cereal grains and forages. If samples could be analyzed without drying and grinding, then sample preparation time and costs may be reduced. This study was conducted to develop robust NIRS equations to predict fermentation quality of corn (Zea mays) silage and to select acceptable sample preparation methods for prediction of fermentation products in corn silage by NIRS. Prior to analysis, samples (n = 112) were either oven-dried and ground (OD), frozen in liquid nitrogen and ground (LN) and intact fresh (IF). Samples were scanned from 400 to 2,500 nm with an NIRS 6,500 monochromator. The samples were divided into calibration and validation sets. The spectral data were regressed on a range of dry matter (DM), pH and short chain organic acids using modified multivariate partial least squares (MPLS) analysis that used first and second order derivatives. All chemical analyses were conducted with fresh samples. From these treatments, calibration equations were developed successfully for concentrations of all constituents except butyric acid. Prediction accuracy, represented by standard error of prediction (SEP) and $R^2_{v}$ (variance accounted for in validation set), was slightly better with the LN treatment ($R^2$ 0.75-0.90) than for OD ($R^2$ 0.43-0.81) or IF ($R^2$ 0.62-0.79) treatments. Fermentation characteristics could be successfully predicted by NIRS analysis either with dry or fresh silage. Although statistical results for the OD and IF treatments were the lower than those of LN treatment, intact fresh (IF) treatment may be acceptable when processing is costly or when possible component alterations are expected.

고압동결고정을 이용한 애기장대 줄기의 cryo-SEM 분석법 (Cryo-SEM Methodology of Arabidopsis thaliana Stem Using High-Pressure Freezing)

  • 최윤정;이경환;제아름;채희수;장지훈;이은지;권희석
    • Applied Microscopy
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    • 제42권2호
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    • pp.111-114
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    • 2012
  • The scanning electron microscopy is an ideal technique for examining plant surface at high resolution. Most hydrate samples, however, must be fix and dehydrate for observation in the scanning electron microscope. Because the microscopes operate under high vacuum, most specimens, especially biological samples, cannot withstand water removal by the vacuum system without morphological distortion. Cryo-techniques can observe in their original morphology and structure without various artifacts from conventional sample preparation. Rapid cooling is the method of choice for preparing plant samples for scanning electron microscopy in a defined physiological state. As one of cryo-technique, high-pressure freezing allows for fixation of native non-pretreated samples up to $200{\mu}M$ thick and 2 mm wide with minimal or no ice crystal damage for the freezing procedure. In this study, we could design to optimize structural preservation and imaging by comparing cryo-SEM and convention SEM preparation, and observe a fine, well preserved Arabidopsis stem's inner ultrastructure using HPF and cryo-SEM. These results would suggest a useful method of cryo-preparation and cryo-SEM for plant tissues, especially intratubule and vacuole rich structure.

Application of Rapid Sample Preparation Method and Monitoring for Cholesterol Content in Chicken Egg and Egg powder

  • Park, Jung-Min;Jeong, In-Seek;Kwak, Byung-Man;Ahn, Jang-Hyuk;Leem, Donggil;Jeong, Jayoung;Kim, Jin-Man
    • 한국축산식품학회지
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    • 제33권5호
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    • pp.672-677
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    • 2013
  • The aim of this study was to develop sample preparation method and evaluate the exact cholesterol content in egg and egg powder purchased from Korean markets, and to determine whether significant differences exist among various egg products, since a variety of products are available in Korean markets and there are no recent databases for cholesterol. To evaluate the cholesterol content in chicken egg sold in Korean local market, a simple method using non-heated saponification to determine cholesterol for emulsified foods was applied. The results of recovery for egg and egg powder were in a range of 92.4-105.0%, with a relative standard deviation between 1.1% and 2.8% by using gas chromatography-flame ionization detector. Therefore, the total cholesterol content in whole egg was estimated between 160.8 and 226.3 mg/egg (AV(average) $186.8{\pm}3.5$), which is similar or lower than previously reported levels. The value for cholesterol in egg powder was estimated between 2.94 and 3.49 mg/g (AV $3.23{\pm}0.15$). We suggested method that can be applicable to chicken egg and egg powder matrix as providing rapid and accurate determination of cholesterol in egg and egg powder. This information will be helpful for processed food producers for deciding food labels of cholesterol content.