• 산업경영시스템학회지
• /
• 제39권4호
• /
• pp.90-96
• /
• 2016

Incremental sheet forming (ISF) is a highly versatile and flexible process for rapid manufacturing of complex sheet metal parts. Compared to conventional sheet forming processes, ISF is of a clear advantage in manufacturing small batch or customized parts. ISF needs die-less machine alone, while conventional sheet forming requires highly expensive facilities like dies, molds, and presses. This equipment takes long time to get preparation for manufacturing. However, ISF does not need the full facilities nor much cost and time. Because of the facts, ISF is continuously being used for small batch or prototyping manufacturing in current industries. However, spring-back induced in the process of incremental forming becomes a critical drawback on precision manufacturing. Since sheet metal, being a raw material for ISF, has property to resilience, spring-back would come in the case. It is the research objective to investigate how geometrical shaping parameters make effect on shape dimensional errors. In order to analyze the spring-back occurred in the process, this study experimented on Al 1015 material in the ISF. The statistical tool employed experimental design with factors. The table of orthogonal arrays of $L_8(2^7)$ are used to design the experiments and ANOVA method are employed to statistically analyze the collected data. The results of the analysis from this study shows that the type of shape and the slope of bottom are the significant, whereas the shape size, the shape height, and the side angle are not significant factors on dimensional errors. More error incurred on the pyramid than on the circular type in the experiments. The sloped bottom showed higher errors than the flat one.

• Journal of Microbiology and Biotechnology
• /
• 제27권4호
• /
• pp.718-724
• /
• 2017

The combination of rabies immunoglobulin (RIG) with a vaccine is currently effective against rabies infections, but improvements are needed. Genetic engineering antibody technology is an attractive approach for developing novel antibodies to replace RIG. In our previous study, a single-chain variable fragment, scFv57R, against rabies virus glycoprotein was constructed. However, its inherent weak stability and short half-life compared with the parent RIG may limit its diagnostic and therapeutic application. Therefore, an acidic tail of synuclein (ATS) derived from the C-terminal acidic tail of human alpha-synuclein protein was fused to the C-terminus of scFv57R in order to help it resist adverse stress and improve the stability and half-life. The tail showed no apparent effect on the preparation procedure and affinity of the protein, nor did it change the neutralizing potency in vitro. In the ELISA test of molecular stability, the ATS fusion form of the protein, scFv57R-ATS, showed an increase in thermal stability and longer half-life in serum than scFv57R. The protection against fatal rabies virus challenge improved after fusing the tail to the scFv, which may be attributed to the improved stability. Thus, the ATS fusion approach presented here is easily implemented and can be used as a new strategy to improve the stability and half-life of engineered antibody proteins for practical applications.

• Journal of Microbiology and Biotechnology
• /
• 제30권3호
• /
• pp.391-397
• /
• 2020

In this study, we used a novel α-L-arabinopyranosidase (AbpBs) obtained from ginsenoside-converting Blastococcus saxobsidens that was cloned and expressed in Escherichia coli BL21 (DE3), and then applied it in the biotransformation of ginsenoside Rb₂ into Rd. The gene, termed AbpBs, consisting of 2,406 nucleotides (801 amino acid residues), and with a predicted translated protein molecular mass of 86.4 kDa, was cloned into a pGEX4T-1 vector. A BLAST search using the AbpBs amino acid sequence revealed significant homology with a family 2 glycoside hydrolase (GH2). The over-expressed recombinant AbpBs in Escherichia coli BL21 (DE3) catalyzed the hydrolysis of the arabinopyranose moiety attached to the C-20 position of ginsenoside Rb₂ under optimal conditions (pH 7.0 and 40℃). Kinetic parameters for α-L-arabinopyranosidase showed apparent K_m and V_max values of 0.078 ± 0.0002 μM and 1.4 ± 0.1 μmol/min/mg of protein against p-nitrophenyl-α-L-arabinopyranoside. Using a purified AbpBs (1 ㎍/ml), 0.1% of ginsenoside Rb₂ was completely converted to ginsenoside Rd within 1 h. The recombinant AbpBs could be useful for high-yield, rapid, and low-cost preparation of ginsenoside Rd from Rb₂.

• The Korean Journal of Ecology
• /
• 제24권5호
• /
• pp.323-332
• /
• 2001

문화경관은 인간의 자연자원이용과 그에 대한 자연의 반응이 조화롭게 어우러져 생긴 산물이다. 자연은 인간의 삶을 보장하기 위하여 그 생존에 필요한 자원 뿐만 아니라 생활에 필요한 자원도 공급하고 있다. 인간은 자연이 주는 이러한 혜택을 이용하여 문명화를 진전시켜 왔다. 얼마전까지 인간과 자연 사이의 관계는 조화의 관계를 유지하여 지속가능한 자원이용을 보장받아 왔고, 우리는 문화경관의 존재를 통하여 그 사실을 발견하고 있다. 그러나 급속도로 진행된 경제성장과 그것에 기인한 개발중심의 논리는 지구환경위기, 각종 자원고갈의 위기, 더 나아가 인간의 정신적 빈곤현상까지 몰고 왔다. 이러한 시점에서 인간과 자연 사이의 조화로운 관계의 전형인 문화경관을 대상으로 그 성립배경과 기능을 검토하고, 새로운 패러다임을 토대로 그 가치를 점검하는 것은 건강한 사회와 경제를 실현하기 위한 새로운 전략을 창조하는데 가치있는 정보를 제공할 수 있다. 본 연구에서는 한국의 문화경관의 성립배경을 검토하였고, 생태적, 경제적 측면에서 그것의 효용과 가치 그리고 문화전승의 장으로서 그 활용방안을 검토하였다.

• 대한치과보철학회지
• /
• 제43권6호
• /
• pp.736-744
• /
• 2005

Statement of problem. Initial stability of implant is an important factor for predicting osseointegration. It requires a rapid, non-invasive, user-friendly technique to frequently assess the implant stability and the degree of osseointegration. Purpose. The aim of this study was to evaluate the correlation between the resonance frequency analysis (RFA) and the radiographic method for peri-implant bone change under in vitro conditions. Material and Method. Twenty implants of 3.75 mm in diameter(Neoplant, Neobiotech, Korea) were used. To simulate peri-implant bone change, 2 mm-deep $45^{\circ}$ range horizontal defect and 2 mm-deep $90^{\circ}$ range horizontal defect area were serially prepared perpendicular to the X-ray beam after conventional implant insertion. Customized film holding device was fabricated to standardize the projection geometry for serial radiographs of implants and direct digital image was obtained. ISQ values and gray values inside threads were measured before and after peri-implant bone defect preparation. Results. Within a limitation of this study, ISQ value of resonance frequency analysis was changed according to peri-implant bone change (p<0.05) and gray value of radiographic method was changed according to peri-implant bone change (p<0.05). There was no correlation between the ISQ value and the gray value for peri-implant bone change (p>0.05). But, in horizontal defect condition, relatively positive correlation were between ISQ and gray values(r=0.663). Conclusion. This results provided a possibility that peri-implant bone change may be evaluated by both RFA and radiographic method.

• 디자인학연구
• /
• 제11권2호
• /
• pp.242-248
• /
• 1998

최근 급속히 증가하고 있는 인터넷 웹 싸이트(InterNet Web Site)들은 멀티미디어를 이용한 다양한 정보 제공과 세계로의 정보 교환 창구로서의 역할을 수행하고 있어, 정보화 사회를 실감할 수 있는 시대에 와 있다. 이러한 상황에서 수 많은 웹 사이트에서 임의의 사용자에게 원하는 정보를 효과적으로 제공하기 위해서는 홈 페이지 표현에 대한 차별화가 우선적으로 요구되는 명제를 가진다. 이 명제를 풀기위한 홈 페이지 디자인에 있어서의 정체성 설정은 필연적이다. 홈 페이지의 정체성 설정을 위하여 정체성은 어떻게 구성되어 있으며, 기존의 홈페이지들은 이런 정체성에 따라 어떻게 반영되어 있는지 분석해 보고, 이 정체성설정을 이용한"쌈지(www.ssamzie.co.kr)'의 홈 페이지 제작 사례를 분석해 봄으로써, 홈 페이지 제작에 있어서 디자이너의 역할이 그래픽 표현을 넘어서 합리적인 차별화를 이루는데 기여하고, 나아가 디자이너가 차지하는 비중을 높임으로써 디자니어의 위상을 제고해 보고자 한다.자 한다.

• 보건의료산업학회지
• /
• 제2권1호
• /
• pp.125-136
• /
• 2008

Government came to enforce long-term care insurance system in preparation for the rapid aging society. Whether this system is successful or not depends on the professionalism of long-term care-givers who are professional population in charge of care service. Currently in the early stage of enforcement, such problems as a race cutting fee resulted from numerical increase of educational facilities, insolvent operation, degradation of education level resulted from unprofessional instructor, are pointed out. As a mean of manpower-training on long-term care insurance system, this study is to research public-private-university network model of the Academy of Continuing Education attached to Ko Sin University which is the case of Yong Do Gu Busan city. Networking between the vision and development strategy of Yong Do Gu on continuing education city, education system on community manpower-training supported by Ko Sin University, and service field of welfare for the elderly can not only contribute to the professionalism of long-term care-givers but also play an ideal role in manpower-utilization and arrangement of community. Through this networking, high quality of education level and circumstance, using the existing infra, manpower-training and utilization for continuing education of Yong Do Gu can be accomplished. Additionally, the connection with facilities related with welfare for the elderly can contribute to professionalism and accountability of manpower-networking.

• 한국식생활문화학회지
• /
• 제3권1호
• /
• pp.89-93
• /
• 1988

국내 도시락 공장을 조사 분석하여 대량생산에 적합토록 그 문제점을 보완 개선했다. 특히 식품위생법과 건축법상의 공장허가 사항을 고려해서 3가지 면적별로 모델 도시락 공장을 설계하였다. 작업장은 하나의 개방공간에 배치된 기기류를 오염지역, 준청결지역, 청결지역으로 나누어 작업중의 2차오염 문제를 배제시켰고, 기기류는 밥급속냉각기, 냉장고 겸 냉각기를 설치하여 위생안전성을 부여했으며, 청결실에 멸균(집진)기를 설치하여 완제품의 초기 미생물 농도를 줄이고 2차오염 요인을 제거함으로써 위생적인 도시락 생산유통이 가능한 도시락 생산공장을 설계하였다.

• 대한방사성의약품학회지
• /
• 제5권2호
• /
• pp.71-78
• /
• 2019

Nuclear imaging is one of the most powerful measures for non-invasive diagnosis of myocardial vascular disease. Radionuclide such as ¹³N, ¹⁵O, ²⁰¹Tl and ⁸²Rb is used for the measurement of cardiac blood flow. ¹³N, ¹⁵O and ²⁰¹Tl are produced in cyclotrons while ⁸²Rb is obtained from generator. Rubidium (Rb), an alkali ion, behaves biologically like potassium, and accumulates in myocardial tissue. Rb has rapid blood clearance profile which allows the use of ⁸²Rb with a short physical half-life of 75 s for non-invasive evaluation of regional myocardial perfusion. There are several advantages of ⁸²Rb over other radioisotopes. An ultra-short half-life significantly reduces the exposure of patients to radiation and allows to repeat injections for studying the effects of medical intervention. As a positron emitter, ⁸²Rb allows positron emission tomography (PET) imaging which have shown superior diagnostic performances. ⁸²Rb can be produced from generator by decay of its parent ⁸²Sr. However, the preparation of ⁸²Sr is difficult, because appropriate purity is required to meet the specification of the product. Recently reported procedure from ARRONAX research institute showed that a Chelex-100 resin is sufficient for this purpose and additional column is not necessary. Whereas Brookhaven National Laboratory (BNL) procedure contains three ion exchange resin separation, including Chelex-100 resin. Currently, since ⁸²Sr production site is non-existent in Korea, Korea Atomic Energy Research Institute (KAERI) has plan to produce ⁸²Sr within specifications. We compared ⁸²Sr purification procedures reported from ARRONAX and BNL to investigate the most suitable procedure for our conditions.

• 한국지하수토양환경학회지:지하수토양환경
• /
• 제15권1호
• /
• pp.57-65
• /
• 2010

Currently, the pursuit of industrialization for rapid economic growth has led to serious environmental damage and related problems because of comtamination with chemicals. Over time, this contaminaion is getting worse and worse. Most developing countries have tried to pursue their economic development and growth, however without preparation for the control and disposal of hazardous wastes. Permitting hazardous waste to be abandoned and disposed in appropriately causes soil pollution, groundwater contamination, and surface water contamination, which are all extremely serious. In particular, when hazardous wastes which include toxic chemicals are distributed around the living environment even in small amounts, concentration can occur in the food chain through ecological systems, which can therefore affect human. Chromium(VI) has contaminated soil in China. We experimented to test the immobilization, chemical reduction, and soil washing methods in order to make our experiment efficient in the purification of soil contaminated with Chromium(VI), and to prove the theoretical models by experiments testing the field applications. The field experiment showed that result of experiment satisfied with the standards of China. The result of multi-stage continuous remediation reduce the Chromium(VI) concentration to 11.95 mg/kg and 14.83 mg/kg that were equivalent to 98% and 97% removal rates. This result implied we could apply multi-stage process of Chromium(VI) contaminated soil to meet the regulatory limit of Chromium(VI).