• Korean Journal of Clinical Laboratory Science
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• 제46권2호
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• pp.64-67
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• 2014

Vancomycin-resistant Enterococci (VRE) are a leading cause of a nosocomial infection. While seven glycopeptide resistance genotypes have been found in Enterococci, vanA and vanB are the most common resistance genotypes. Aims of this study were to detect antibiotic susceptibilities of 23 Enterococcus spp, which broke out in a university hospital by the disk diffusion test, to investigate specific genes of vanA and vanB by conventional and real-time PCR. PCR for vanA and vanB was performed on 23 Enterococci, all 23 were positive for vanA type. This study reports the validation of a simple and rapid VRE detection method that can be easily incorporated into the daily routine of a clinical laboratory. Early detection of VRE strains, including those with susceptibility to Vancomycin, is of paramount clinical importance, as it allows a rapid initiation of strict infection control practices as well as a therapeutic guidance for a confirmed infection. The real-time PCR method is a rapid technique to detect vanA in Enterococci. It is simple and reliable for the rapid characterization of VRE.

• Proceedings of the Korean Geotechical Society Conference
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• 한국지반공학회 2000년도 가을 학술발표회 논문집
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• pp.681-688
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• 2000

This study presented the new method for evaluating the coefficient of consolidation by using inflection point method which was based on the fact that time factor, T corresponding to the inflection point of a semilogarithmic plot of a time curve is fixed and equals to T ＝ 0.405 at 70% consolidation. In the proposed method, the next load increment is applied as soon as the necessary time required to identify the inflection point. Thus, the coefficient of consolidation may be easily evaluated. The time required to complete the testing using this rapid consolidation method could be as low as 1.5-3 hours compared with 1 or 2 weeks in the case of the conventional consolidation test.

• Journal of Microbiology and Biotechnology
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• 제12권2호
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• pp.345-348
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• 2002

A rapid spectrophotometric method for detecting the mucinase complex was developed. Bovine submaxillary mucin is cleaved by commercial mucinase between the oligosaccharide chain and the side chain of peptide linkage, thereby liberating the N-acetyl neuraminic acid (NANA). The release of NANA resulted in an increase of absorbance at 280 nm. The susceptibility to NANA by the new method was found to be at least 10-fold more sensitive than the thiobarbituric acid method. Moreover, the quantification of NANA released from mucin by commercial neuraminidase and partially purified Vibrio parahaemolyticus mucinase showed a good linear correlation in proportion to the concentration of the enzyme used. These results demonstrate that the rapid identification of mucin degradation can be determined by a spectrophotometric assay, thereby providing a new, fast, and sensitive method for assaying the bacterial mucinase complex.

• Clinical and Experimental Reproductive Medicine
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• 제26권2호
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• pp.127-135
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• 1999

Cryopreservation is able to store the surplus pre-embryos for freezing and furthermore thawing and transfer in a subsequent cycle. Cryopreserving cells which are maintaining their viability are the very complex process. This study has been carried out in order to find the effects of cryopreservation steps, freezing media and embryonic stages on the rates of viability and development of cryopreserved mouse embryos. Female ICR mice ($6{\sim}8$ weeks old) were induced to superovulate by sequential intraperitoneal injection of 5 IU PMSG and 5 IU hCG 48h apart. Mouse embryos were collected according to its developmental stage after the injection of hCG. Embryos were cryopreserved not only by cryoprotectant step (1 step${\sim}$4 step) but also in a variety of media (HTF, IVF medium, D-PBS) and cell stage. The results were as follows: There is no clear advantage in these freezing media of rapid method, but 4 cell and 8 cell of slow method (2, 3, 4 step) have advantage in D-PBS. The development of embryos according to cell stage become greater in 8 cell stage. In the treatment steps of cryopreservation, the development of embryo to blastocyst was similar among rapid method, but the development of 4 cell and 8 cell embryos to blastocyst according to slow method was better than rapid method.

• Earthquakes and Structures
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• 제14권6호
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• pp.615-626
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• 2018

The seismic vulnerability of Turkey is relatively high due to its active fault systems with potential to create destructive earthquakes. Thus, reducing the loss of life and property, the number of the earthquake-prone buildings and their retrofit requirements are considerably significant key issues under the scenario earthquakes. The street survey based rapid assessment (SSRA) method can be considered as a powerful tool to determine the seismic vulnerability of building stock of an earthquake-prone city/state. In this study, the seismic vulnerability of the building stock of the Kirikkale province in Turkey is aimed to be estimated adopting the street survey based rapid assessment method (SSRA). For this purpose, central 2074 existing reinforced concrete (R/C) buildings were structurally surveyed with rapid visual site screening and disadvantages such as, the existence of short-column, soft-story, heavy overhangs, pounding effect and local soil conditions were determined for obtaining the structural performance score of each. The results obtained from the study demonstrate that 11-25% of the surveyed buildings in the study region needs to be investigated through more advanced assessment methods. Besides, higher correlation between increasing story number and unsafe/safe building ratio is obtained for the buildings with soft-story parameter than that for those with heavy overhangs and short-column parameters. The conformity of the results of the current study with the previous documented cases of rapid assessment efforts in the recent earthquakes in Turkey shows that the SSRA method for the Kirikkale province performed well, and thus this methodology can be reliably used for similar settlement areas.

• Journal of the Korean Society for Precision Engineering
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• 제16권6호
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• pp.52-57
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• 1999

Funtional metal prototypes are often required in numerous industrial applications. These components are typically needed in the early stage of a project to determine form, fit and function. Recent R/P(Rapid Prototyping) part are made of soft materials such as plastics, wax, paper, these master models cannot be employed durable test in real harsh working environment. Parts by direct metal rapid tooling method, such as laser sintering, by now are hard to get net shape, pores of the green parts of powder casting method must be infiltrated to get proper strength as tool, and new type of 3D direct tooling system combining fabrication welding arc and cutting process is reported by song etc. But a system which can build directly 3D parts of high performance functional material as metal part would need long period of system development, massive investment and other serious obstacles, such as patent. In this paper, through the rapid tooling process as silicon rubber molding using R/P master model, and fabricate wax pattern in that silicon rubber mold using vacuum casting method, then we tranlsated the wax patterns to numerous metal prototypes by new investment casting process combined conventional investment casting with rapid pototyping & rapid tooling process. with this wax-injection-mold-free investment casting, we developed new investment casting process of fabricating numerous functional metal prototypes from one master model, combined 3-D CAD, R/P and conventional investment casting and tried to expect net shape measuring total dimension shrinkage from R/P part to metal part.

• Journal of the Korean Society for Precision Engineering
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• 제27권2호
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• pp.40-49
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• 2010

The Purpose of this paper is to weld a rapid palatal expander using a continuous wave Nd:YAG laser. The rapid palatal expander has become a useful treatment method for severe maxillary transverse deficiencies and posterior crossbites. Rapid maxillary expansion is a well-established method to correct transverse maxillary deficiency and arch length discrepancy. The major process parameters studied in the present laser welding experiment were the positions of focus, laser power and travel speed of laser beam. We measured the fusion zone size and its shape using an optical microscope for the observation of cross-sectional area and tension stress of a rapid palatal expander welded. Through the experimental investigation, the optimum speeds and power of laser without deficiencies of weld cross-sectional area were obtained.

• Journal of the Korean Geotechnical Society
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• 제33권12호
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• pp.115-125
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• 2017

PHC-W earth retaining wall could be constructed continuously. Various retaining wall methods such as C.I.P. etc. method require separate waterproof method. However, the PHC-W retaining wall method prevents leakage of groundwater by inserting a waterproofing material at connection part between 2 PHC piles. In this study, the experimental study on 3 waterproofing method for PHC-W retaining wall was conducted at the model pressure chamber. In the method using textile with 1-liquid type and 2-liquid type urethane, rapid leak occurred at the pressure of 120 kPa and 140 kPa or more. In the method of textile with grouting, rapid leak occurred at the pressure of 120 kPa or more, however, in this method, the rapid leakage happened at the top part and the bottom part reinforced with urethane.

• Journal of Microbiology and Biotechnology
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• 제19권11호
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• pp.1464-1469
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• 2009

To achieve more accurate and rapid detection of macrolide-lincosamide-streptogramin B resistance genes, erm(A), erm(B), and erm(C), we developed a TaqMan probe-based real-time PCR (Q-PCR) method and compared it with conventional PCR (C-PCR), which is the most widely using erm gene identification method. The detection limit of Q-PCR was 5 fg of genomic DNA or 5-8 CFU of bacterial cells of Staphylococcus aureus. The utilization of Q-PCR might shorten the time to erm detection from 3-4 h to about 50 min. These data indicated that Q-PCR assay appears to be not only highly sensitive and specific, but also the most rapid diagnostic method. Therefore, the appropriate application of the Q-PCR assay will permit rapid and accurate identification of erm genes from clinical and other samples.

• Korean Journal of Food Science and Technology
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• 제29권6호
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• pp.1327-1329
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• 1997

A new rapid saponin extraction method was developed with using of organic solvent and waring blonder. There was a good correlation between previous distillation method and this method in f major ginsenosides ($Rb_1$, $Rb_2$, Rc, Rd, Re, Rg1) contents. When the ratio of methanol and chloroform was 7:3, this method showed similar saponin contents (total major. ginsenosides contents) comparing with distillation method. Contents of total major ginsenosides were 2.41% in this method and 2.54% in distillation method. However, crude saponin content of this method was higher than that of distillation method.