• Journal of Korean Society of Water and Wastewater
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• v.25 no.3
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• pp.367-373
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• 2011

Mechanism of rapid mixing effect on chemical phosphorus removal is evaluated in this study. Assuming that chemical phosphorus removal is unaffected by mixing time, only rapid mixing intensity is evaluated. In order to find out the mechanism, it is hypothesized that rapid mixing affects the Al hydrolysis speciation, and that formation of more monomeric species ($Al^a$) results in better removal of phosphorus. According to a ferron assay, more $Al^a$ formed at higher mixing intensity than at lower intensity. Subsequent experiments revealed that better phosphorus removal was obtained at higher intensity than at lower intensity, in terms of the molar ratio of $Al_{added}/P_{removed}$. The proposed hypothesis was proved in this study. Chemical phosphorus removal is affected by rapid mixing intensity due to its effect on the Al hydrolysis speciation.

• Bulletin of the Korean Chemical Society
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• v.27 no.2
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• pp.224-230
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• 2006

Escherichia coli transcription termination factor Rho catalyzes the unwinding of RNA/DNA duplex in reactions that are coupled to ATP binding and hydrolysis. We report here the kinetic mechanism of presteady state ATP binding and hydrolysis by the Rho-RNA complex. Presteady state chemical quenched-flow technique under multiple turnover condition was used to probe the kinetics of ATP binding and hydrolysis by the Rho-RNA complex. The quenched-flow presteady state kinetics of ATP hydrolysis studies show that three ATPs are bound to the Rho-RNA complex with a rate of $4.4\;{\times}\;10^5M^{-1}s^{-1}$, which are subsequently hydrolyzed at a rate of $88s^{-1}$ and released during the initiation process. Global fit of the presteady state ATP hydrolysis kinetic data suggests that a rapid-equilibrium binding of ATP to Rho-RNA complex occurs prior to the first turnover and the chemistry step is not reversible. The initial burst of three ATPs hydrolysis was proposed to be involved in the initialization step that accompanies proper complex formation of Rho-RNA. Based on these results a kinetic model for initiation process for Rho-RNA complex was proposed relating the mechanism of ATP binding and hydrolysis by Rho to the structural transitions of Rho-RNA complex to reach the steady state phase, which is implicated during translocation along the RNA.

• Fisheries and Aquatic Sciences
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• v.2 no.1
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• pp.36-43
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• 1999

A study on the hydrolysis of anchovy using proteolytic bacteria isolated from anchovy jeotkal (a salt-fermented fish) was carried out to develop a rapid process of liquefied anchovy jeotkal. Five kinds of proteolytic bacteria, such as Staphylococcus sp.-l, Photobacterium sp., Volcaniella sp., Staphylococcus sp.-2 and Bacillus sp., were isolated from the anchovy jeotka1 that fermented with $20\%$ NaCl at room temperature for 2 months. Those grew well at $40^{\circ}C$, pH 7.0 on TPY broth with $2.0\%$ NaCl. The optimal hydrolysis temperature, pH, time and proteolytic bacteria densities for hydrolysis of minced anchovy were$40^{\circ}C$, 7.0, 6 hours and $1.8\times10^8$ cells/g raw anchovy, respectively.

• Journal of Ginseng Research
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• v.17 no.1
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• pp.35-38
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• 1993

A new and rapid method for the hydrolysis of ginsenosides to panaxadiol or panaxatriol was developed. It is based on the microwave oven reaction, which is high temperature and high-pressure reaction. The optimal hydrolysis time using 5% $H_2SO_4$ solution was found at 10 min PTFE reaction vessel in microwave oven, which is more than 30 times faster than the conventional hydrolysis method.

• Journal of the Korean Ceramic Society
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• v.28 no.2
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• pp.167-175
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• 1991

In synthesizing hydrated zirconia powder by hydrolysis of Zr-alkoxides using ethanol as mutual solvent, three experimental parameters, namely, concentration of alkoxides and hydrolysis water and addition rate of hydrolysis water were varied systematically. Spherical, monodispersed, nonagglomerated and submicrometer sized powders were prepared at 0.3 M of Zr(n-OPr)4 and 0.05M of Zr(n-OBu)4 with wide ranges of hydrolysis water conditions i.e. 0.5-2.0M concentration and 1-20ml/min addition rate. During the hydrolsis, careful attention have to be paid to maintain homogeneous reaction by controlling the agitation of the reactant and the addition of the hydrolysis water. For more improved condition of monodispersity it was found that the key point is to shorten the self-nucleation time within several seconds as rapid as possible. In both alkoxides system, with higher concentration of alkoxide and hydrolysis water and with slow addition rate of hydrolysis water, hydrated zirconia powders synthesized showed tendency to fall in worse powder conditions.

• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.5
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• pp.788-793
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• 1997

Addition of sugar, enzymatic hydrolysis and salt concentration were evaluated for their effects on the changes in some characteristics of Kimchi juice during fermentation. The Kimchi juice was prepared by brining and grinding of outer layer leaves of chinese cabbage, one of the wastes products of Kimchi processing, followed by fermentation proceeded significantly faster. Addition of sucrose or glucose at the ange of 0.5~2.0 % also improved the fermentation but the concentration effect was little. Enzymatic hydrolysis on the brined cabbage prior to fermentation with a commercial polysaccharides hydrolases also increased the fermentation. However the solid concentration in Kimchi juice was rather decreased by higher concentration of NaCl and enzymatic hydrolysis. The reducing sugar content showed a rapid decrease from 24 hours of fermentation and the effect of enzymatic hydrolysis was little.

• Journal of the Korean Ceramic Society
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• v.59
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• pp.527-535
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• 2020

This study reports the preparation of the Candida antarctica lipase B (CalB) enzyme immobilization on silica-coated magnetic nanoparticles (Si-MNPs@CalB) using various cross-linkers and demonstration of rapid catalytic hydrolysis of p-nitrophenyl alkyl esters. CalB enzymes were coupled with different cross-linker silanes on the Si-MNPs surface. Among these cross-linkers, Cl-functionalized silane was better at immobilization of CalB than the others. Catalytic hydrolysis of p -nitrophenyl alkyl esters was demonstrated against Si-MNPs@CalB as a function of the length of alkyl chain (C₄, C₈, C₁₂, and C₁₆). From the Michaelis-Menten equation and Lineweaver-Burk plots, various enzyme kinetic parameters (i.e., K_m, V_max, and K_cat) were calculated. Catalytic hydrolysis was faster in shorter alkyl chain of p-nitrophenyl alkyl esters with Si-MNPs@CalB in the order C₄>>C₈>C₁₂>>C₁₆. Furthermore, the reusability and optimum catalytic activity of Si-MNPs@ CalB were evaluated as a function of the number of reuses and with different pH values.

• Journal of Microbiology and Biotechnology
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• v.2 no.3
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• pp.197-203
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• 1992

Studies of the pretreatment of chitin and its subsequent hydrolysis by Aspergillus carneus chitinase are reported. Ball milling was found to be the most effective way among the pretreatment methods tested. Data are presented describing the effect of enzyme and substrate concentrations on the rate and extent of the hydrolysis process. It was found that the successive addition of enzyme improved the saccharification yield. Significant product inhibition of the chitinase was observed when N-acetylglucosamine concentration was 3.6％ or higher. Adsorption of enzymes to the substrate occurred during a 24 hr hydrolysis period. An initial rapid and extensive adsorption occurred, followed by gradual desorption which increased during the time of reaction. Intermediate removal of the hydrolyzate and continuation of the hydrolysis by adsorbed enzyme on the residual chitin was also investigated. A total of 75.4 g/l reducing sugars, corresponding to 69.2％ saccharificaton yield (as N-acetylglucosamine) was obtained. In addition an increase in the amount of recoverable enzymes was observed under these conditions. Evidence presented here suggests that the technique, whereby the free enzymes in the recovered hydrolyzate are re-adsorbed onto the new substrate, may provide a means of recirculating the dissolved enzymes.

• Bulletin of the Korean Chemical Society
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• v.27 no.10
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• pp.1618-1622
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• 2006

Bacteriophage T7 gp4A' is a ring-shaped hexameric DNA helicase that catalyzes duplex DNA unwinding using dTTP hydrolysis as an energy source. To investigate the effect of single-stranded DNA (ssDNA) on the kinetic pathway of dTTP hydrolysis by the T7 DNA helicase complexed with ssDNA, we have first determined optimal concentration of long circular M13 single-stranded DNA and pre-incubation time in the absence of $Mg^{2+}$ which is necessary for the helicase-ssDNA complex formation. Steady state dTTP hydrolysis in the absence of $Mg^{2+}$ by the helicase-ssDNA complex provided $k_{cat}$ of $8.5\;{\times}\;10^{-3}\;sec^{-1}$. Pre-steady state kinetics of the dTTP hydrolysis by the pre-assembled hexameric helicase was monitored by using the rapid chemical quench-flow technique both in the presence and absence of M13 ssDNA. Pre-steady state dTTP hydrolysis showed distinct burst kinetics in both cases, indicating that product release step is slower than dTTP hydrolysis step. Pre-steady state burst rates were similar both in the presence and absence of ssDNA, while steady state dTTP hydrolysis rate in the presence of ssDNA was much faster than in the absence of ssDNA. These results suggest that single-stranded DNA stimulates dTTP hydrolysis reaction by T7 helicase by enhancing the rate of product release step.

• Korean Chemical Engineering Research
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• v.44 no.5
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• pp.547-554
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• 2006

The overall objective of this research was to find out the role of rapid mixing conditions in the species of hydrolyzed Al(III) formed by Al(III) coagulants and to evaluate the distribution of hydrolyzed Al(III) species by coagulant dose and coagulation pH. When an Al(III) salt was added to water, monomeric Al(III), polymeric Al(III), precipitate Al(III) was formed by Al(III) hydrolysis. The method of hydrolyzed Al(III) species characterization analysis was based on timed spectrophotometer with ferron as a color developing reagent. The hydrolytic species were divided into monomer, polymer, precipitate from the reaction kinetics. And then, the color intensity for monomeric Al(III) was read 3 min after mixing. With standard Al solution containing monomeric Al(III) only, the Al-ferron color intensity slightly increased with until about 3 min. During the rapid mixing period, for purewater, formation of dissolved Al(III) (monomer and polymer) was similar to rapid mixing condition, but for raw water, the species of Al(III) hydrolysis showed different result. During the rapid mixing period, for high coagulant dose, Al-ferron reaction increases rapidly. The kinetic constants, Ka and Kb, derived from Al-ferron reaction. The kinetic constants followed very well the defined tendencies for coagulation condition. For pure water, when the rapid mixing time increased, the kinetic constants, Ka and Kb showed lower values. Also, for raw water, when the rapid mixing time increased, the kinetic constants, Ka and Kb showed lower values.