• Journal of Microbiology
• /
• 제34권3호
• /
• pp.229-235
• /
• 1996

Microgram quantities of DNA per gram soil were recovered with SDS- based and freeze-and thaw procedures. The average DNA fragment size was > 23 Kb. This method generated minimal shearing of extracted DNA. However, the DNA extracts still contained considerable amounts of humic impurities sufficient to inhibit PCR. Several approaches were used to reduce the interferences with the PCR (use of CTAF in extraction step, Elutip-d column purification, addition of BSA to PCR buffer) to accomplish PCR with DNA extract as a template. Most of the DNA extracts were not digested completely by restriction endonuclease, and CTAB-TREATED ane Elutip-d column purified DNA extracts were partially digested. Regarding as restriction enzyme digestion, all PCRs failed to amplify 16S rRNA gene fragments in the DNA extracts. In the case of DNA extracts only where BSA was added to PCR buffer, PCR was successfully conducted whether the DNA extracts were treated with CTAB or purified with columns. However, these two treatments were indispensable for humic impurity-rich DNA extracts to generate the PCR-compatible DNA samples. Direct extraction of DNA, coupled with these procedures to remove and relieve interferences by humic impurities and followed by the PCR, can be rapid and simple method for molecular microbiological study on soil microorganisms.

• 한국식품영양학회지
• /
• 제22권3호
• /
• pp.352-356
• /
• 2009

흑후추와 백후추 원두에서 HPLC에 의한 piperine 함량, 가열처리 전후의 시료에 대한 Soxhlet법을 이용한 휘발성 기름성분 함량, SDE(Linkens-Nikens type simultaneous steam distillation and extraction apparatus)법을 이용한 총 휘발성 물질 함량 등을 측정하였다. 그 결과, 흑후추와 백후추의 구성성분차이, 인도네시아와 인도의 산지 기후조건 및 품종 차이 등에 따른 편차는 인정되었으나 전체적으로 일정한 범위 내에서 차이를 나타내었다. 후추에서 맛의 차이는 맛 성분의 차이보다는 휘발성 물질의 함량 차이가 큰 영향을 미쳐 냄새를 일반적으로 맛으로 착각하는 경향이 있는 것으로 판단된다. 이로부터 상호간의 측정결과를 비교평가해 본 결과 일정 수준의 비례적 관계를 갖는 것으로 나타나 현장의 품질관리 등에서 간편하고 신속하게 결과를 측정할 수 있는 Soxhlet법에 의한 휘발성 기름 성분 함량 측정법을 후추의 중요한 품질평가 방법의 하나로 활용할 수 있을 것으로 기대되는 결과를 얻었다.

• 한국항해항만학회:학술대회논문집
• /
• 한국항해항만학회 2022년도 춘계학술대회
• /
• pp.277-279
• /
• 2022

Rapid Development and adoption of AIS as a survailance tool has resulted in widespread application of data analysis technology, in addition to AIS ship trajectory clustering. AIS data-based clustering has become an increasingly popular method for marine traffic pattern recognition, ship route prediction and anomaly detection in recent year. In this paper we propose a route waypoint extraction by clustering ships CoG variance trajectory using Density-Based Spatial Clustering of Application with Noise (DBSCAN) algorithm in both port approach channel and coastal waters. The algorithm discovers route waypoint effectively. The result of the study could be used in traffic route extraction, and more-so develop a maritime anomaly detection tool.

• 한국동물위생학회지
• /
• 제41권2호
• /
• pp.105-110
• /
• 2018

A total of 41 Salmonella (S.) strains were isolated from pigs suffered with severe watery diarrhea and were tried to identify by both matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and polymerase chain reaction (PCR) analysis. Fibrinous exudate and ulceration in the large intestine were prevalent in gross observation, and variable degrees of enteritis were observed in the histology of large intestines. Subsequent polymerase chain reaction (PCR) analyses demonstrated that 41 strains were identified as S. Typhimurium (39 strains), though 2 stains were failed to identify. Further identification was performed using both direct smear and protein extraction method by MALDI-TOF MS analyses. In terms of extraction methods, 100% (41/41) of isolates were identified to species level of S. spp. Whereas only 43.9% (18/41) were identified to species level using the direct method. These results thus suggest that rapid and accurate diagnosis of porcine salmonellosis can be guaranteed by MALDI-TOF MS combined with protein extraction method.

• 정보처리학회논문지B
• /
• 제8B권6호
• /
• pp.705-717
• /
• 2001

이 논문에서는 카메라로 연속으로 촬영한 일련의 그레이레벨 영상으로부터 전방의 차량을 포착하기 위한 빠른 차선검출방법을 제안한다. 개별 영상에서 가려지지 않는 제한된 영역을 대상으로 차선의 위치를 검출하고, 에지 영상을 이용하여 차선의 기울기를 구한다. 이를 근거로 차량이 존재할 가능성이 있는 관심영역을 구하고 그 영역 내에서 에지 성분을 이용하여 구조적 방법으로 전방 차량의 위치를 포착한다. 제안된 방식의 효과를 검증하기 위해 노트북 PC와 PC용 CCD 카메라로 도로에서의 영상을 촬영하고 차선검출알고리즘을 적용한 처리 시간, 정확도, 차량검지 등의 결과를 보인다.

• Bulletin of the Korean Chemical Society
• /
• 제25권10호
• /
• pp.1489-1494
• /
• 2004

A gas chromatography/mass spectrometric assay method was developed for the determination of persistent organochlorine pollutants (POPs) in hair. For the exact extraction study was used hair of rat exposed with POPs. Sonication of the hair matrix with 3 M HCl solution in methylene chloride of the extraction methods studied was the most efficient and rapid sample preparation method. After sonication of rat hair was achieved clean up with a solid phase extraction procedure using silica gel-florisil. Elution was performed with 8 mL of methylene chloride. The eluate was concentrated to approximately 100 ${\mu}L$ and analyzed by gas chromatography-mass spectrometry (GC-MS). Detection limits of POPs were in the concentration range of 0.6-1.2 ng/g in rat hair. Aldrin, dieldrin, p,p-DDT and mirex were dosed rat for 4 weeks at concentration of 0.01 mg/L in drinking water and detected in rat hair at concentration of 2.8, 11.3, 7.9 and 15.6 ng/g, respectively. Aldrin and p,p-DDT were metabolized to dieldrin and p,p-DDE, which were detected in concentration of 9.7 and 2.9 ng/g in rat hair, respectively. The developed method may be valuable to be used to analyze POPs in human hair.

• 분석과학
• /
• 제21권3호
• /
• pp.174-182
• /
• 2008

본 연구에서는 PCBs 함유 폐기물의 적정관리방안을 마련하기 위하여 우리나라의 폐기물공정시험방법에서 제시하고 있는 액상폐기물 중 PCBs 분석방법을 토대로 시간 및 비용을 효과적으로 보다 신속하게 분석하는 방법을 제시하고자, 추출 및 전처리방법, 칼럼정제방법, 분석용 칼럼 조건, 기기분석 조건 및 정량방법을 개발하였다. 분석방법을 검토한 결과 분석시간을 폐기물공정시험방법의 분석시간의 2/3정도로 단축시켰고, 검출한계는 0.5 mg/L이었다. 또한, 제안된 신속분석방법을 PCBs 함유 절연유에 적용하였을 경우 규제기준(2.0 mg/L) 부근인 2.35 mg/L에서 과소평가되는 경향이 나타났으며, 이 시험법을 사용하였을 때 PCBs 함유 폐기물의 부적정하게 평가되는 것을 막기 위해 적용 농도범위를 소수첫째자리에서 반올림하여 최소자리수를 일의자리 즉 1 mg/L 이하인 경우(0.5~1.4 mg/L)로 제한하는 것이 바람직한 것으로 나타났다.

• Journal of Microbiology and Biotechnology
• /
• 제27권9호
• /
• pp.1593-1601
• /
• 2017

Vibrio species are generally recognized as pathogens predominant in seafood along coastal areas. The food industry has sought to develop efficient microbial detection methods. Owing to the limits of conventional methods, this study aimed to establish a rapid identification method for Vibrio isolated from Korea, based on matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Four different preparation procedures were compared to determine the appropriate means to pretreat Vibrio species, using 17 isolates and five reference strains. Extended direct transfer and full formic acid extraction methods using bacterial colonies on agar plates revealed very low identification rates. Formic acid and trifluoroacetic acid (TFA) extractions using bacterial broth cultures were also performed. All Vibrio isolates and reference strains prepared by TFA extraction were successfully identified to the species level (17/22, 77.3%) and to the genus level (5/22, 22.7%). Thus, TFA extraction was considered the most appropriate method to pretreat Vibrio species for MALDI-TOF MS. The remaining 33 isolates and two reference strains were prepared by TFA extraction and analyzed by MALDI-TOF MS. Overall, 50 isolates were identified to the species level (40/50, 80%) and to the genus level (10/50, 20%). All isolates were identified as 43 V. alginolyticus, six V. parahaemolyticus, and one V. vulnificus species. V. alginolyticus and V. parahaemolyticus were isolated from fish offal (87.5% and 12.5%, respectively), seawater (91.3%, 8.7%), and shellfish (62.5%, 37.5%), whereas V. alginolyticus and V. vulnificus were isolated from sediment (90.9% and 9.1%, respectively). This study established a reliable system of MALDI-TOF MS preparation and analysis for Vibrio identification.

• 한국식품저장유통학회지
• /
• 제3권2호
• /
• pp.131-136
• /
• 1996

Even though plum is well known as a health food, the available processed food of it is quite limited. Furthermore it is difficult to find an easy processing method for leading to a value added product at farmhouse. This study was investigated to effects of temperature for plum extract juice by osmosis of yellow sugar. The plum was mixed with yellow sugar at equal ratio(w/w) to obtain yellowish extracted plum juice and then located in incubator at 15, 25, 35$^{\circ}C$, respectively. The sugar content of extract was high at the early period but decreased throughout extraction period and it was about 66.1~67.4$^{\circ}$Brix. The color difference was increased yellowish throughout the extract period. The pH of it was decreased throughout extract processing but citric acid content was increased. The speed of extraction during extraction processing was more rapid at 15$^{\circ}C$ than 3$0^{\circ}C$. However sensory evaluation score of taste and flavour was obtained at 15$^{\circ}C$ than 3$0^{\circ}C$, so the prefer temperature for plum extract juice by osmosis of yellowish sugar was 15~2$0^{\circ}C$.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• 제16권3호
• /
• pp.771-791
• /
• 2022

Protein-protein interaction (PPI) extraction from original text is important for revealing the molecular mechanism of biological processes. With the rapid growth of biomedical literature, manually extracting PPI has become more time-consuming and laborious. Therefore, the automatic PPI extraction from the raw literature through natural language processing technology has attracted the attention of the majority of researchers. We propose a PPI extraction model based on the large pre-trained language model and adversarial training. It enhances the learning of semantic and syntactic features using BioBERT pre-trained weights, which are built on large-scale domain corpora, and adversarial perturbations are applied to the embedding layer to improve the robustness of the model. Experimental results showed that the proposed model achieved the highest F1 scores (83.93% and 90.31%) on two corpora with large sample sizes, namely, AIMed and BioInfer, respectively, compared with the previous method. It also achieved comparable performance on three corpora with small sample sizes, namely, HPRD50, IEPA, and LLL.