• Journal of Microbiology and Biotechnology
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• v.31 no.10
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• pp.1446-1454
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• 2021

Herein, we cloned and expressed an endo-β-1,4-glucanase gene (celA1805) from Bacillus subtilis B111 in Escherichia coli. The recombinant celA1805 contains a glycosyl hydrolase (GH) family 8 domain and shared 76.8% identity with endo-1,4-β-glucanase from Bacillus sp. KSM-330. Results showed that the optimal pH and temperature of celA1805 were 6.0 and 50℃, respectively, and it was stable at pH 3-9 and temperature ≤50℃. Metal ions slightly affected enzyme activity, but chemical agents generally inhibited enzyme activity. Moreover, celA1805 showed a wide substrate specificity to CMC, barley β-glucan, lichenin, chitosan, PASC and avicel. The K_m and V_max values of celA1805 were 1.78 mg/ml and 50.09 µmol/min/mg. When incubated with cellooligosaccharides ranging from cellotriose to cellopentose, celA1805 mainly hydrolyzed cellotetrose (G4) and cellopentose (G5) to cellose (G2) and cellotriose (G3), but hardly hydrolyzed cellotriose. The concentrations of reducing sugars saccharified by celA1805 from wheat straw, rape straw, rice straw, peanut straw, and corn straw were increased by 0.21, 0.51, 0.26, 0.36, and 0.66 mg/ml, respectively. The results obtained in this study suggest potential applications of celA1805 in biomass saccharification.

• English & American cultural studies
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• v.14 no.1
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• pp.73-94
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• 2014

Accounts from therapeutic culture seem often to associate the selfish, or at least self-centered quest for self-fulfillment with individual choice or satisfaction, self-expression, expressive individualism, and emotionalism. These associations point to the downside of therapy as they present it as constituting a culture of narcissism, selfishness, or irresponsibility. While some of these characterizations contain useful insights, they overlook what are maybe some of the most important features of a therapeutic outlook. This paper aims to reveal that the therapeutic imperative is not so much geared towards the realization of self-fulfillment, as it is the promotion of self-limitation. Therapeutic culture tends to posit the self in a fragile and feeble form and insist that the management of life requires the continuous intervention of therapeutic expertise. Because of this, the elevated concern with the self is underpinned by anxiety, pain, suffering, and survival, rather than seen as a positive vision of realizing the human potential. Therapeutic culture has in this way helped to construct a diminished sense of self by which one is seen as suffering from an emotional deficit and vulnerability. This paper demonstrates this downside of therapeutic culture concerning self-limitation and the sense of a diminished self by examining popular "misery memoirs." Misery memoirs are widely consumed by the general public, therefore tend to be treated by contemporary therapeutic culture as a gospel on the therapeutic ideal for self-fulfillment and self-discovery. This is, despite the existence of hidden evidence to the contrary, because of their redemptive, happy endings that show individuals overcoming difficult trials such as child abuse, incestuous rape, and domestic violence. Individual self-fulfillment and self-discovery in such stories are not achieved through the active agency of the subject but through the passive endurance of pathological symptoms and with the aid of expertise and outside support. Therefore, such stories put victims in the limited position.

• The Journal of the Convergence on Culture Technology
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• v.7 no.4
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• pp.363-368
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• 2021

The purpose of this research is to analysis the effectiveness of education in which sexual violence offenders participated in correctional program. The subjects of this study were men living in D city had completed 40 hours of correctional program implemented by the Ministry of Justice. For data collection, a pre-test before training and a post-test completion of training were conducted for those who participated in the program. Those who had aggression, impulsivity, rape myth and anger had a positive effect after the correctional program, but that program didn't help those with feelings of loneliness. This study analyzed the effectiveness of the education through pre and post tests of the Ministry of Justice's sexual violence program targeting sexual violence offenders, and it is significant in seeking ways to change their behavior accordingly. Therefore, this study will be effective in preventing the recurrence of sexual violence, and it will contribute as a data that can be used for the development of the correctional program for sexual violence offenders.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.20
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• pp.100-106
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• 1975

Germinating ability of green seed in maturing period was investigated to develop the technics on generation shortening. Rapeseed did not germinate until 40 days after flowering (=DAF) in green seed and 50 DAF in dried seed. Effective germination to be utilized for generation shortening came on 55 DAF for dried seed by heating and on 75 DAF for green seed. The periodic time of seed dormancy in maturing period had two cycles and rest period was considered that it was different according to the ecological types of early, middle and late maturity.

• Korean Journal of Culture and Social Issue
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• v.24 no.3
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• pp.365-383
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• 2018

In this study, to search for events that cause high levels of PTSD symptoms, traumatic events are classified into 'criterion events' that meet DSM-IV-TR criteria and 'life stresssful events', conditional probability of PTSD was confirmed. From a sample of 1,000 adults residing in South Korea, 998 statistically relevant samples were extracted. Criterion events include cases of 'sexual harassment before age 16', 'sightings of other accidents', 'rape before 16', 'domestic violence before 16', 'disaster', 'traffic accidents', 'other accidents'. Life stressful events appeared to be 'legal arrest or detention(person and family)', 'parental separation or divorce', 'failure or despair causing serious stress', 'extreme conflict with family or frequent quarrels'. Among the demographic characteristics, age, marital status, religion were found to affect PTSD symptoms. The implications and limitations of these results are discussed.

• Journal of Auto-vehicle Safety Association
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• v.15 no.3
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• pp.7-16
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• 2023

In 2019, a child died by a school zone traffic accident in Asan, Chungcheongnam-do, the Republic of Korea. Just after the accident, under the name of the "Minsik Law", the Road Traffic Act and the Specific Crime Aggravated Punishment Act were partially revised and went into effect in Korea on March 25, 2020. The new Korean law providing for harsh penalties is designed to reduce automobile accidents in school zones. However, the penalties under the new law seems to be unconstitutionally and unduly harsh. Under the new law, a negligent driver who kills a child at a school zone could be sentenced to indefinite imprisonment, or imprisonment for 3 years or more. The criminal responsibility of a negligent driver at a school zone is the same as serious intentional felonies such as rape, robbery, abandonment resulting in death. Also, even in the case of a school zone accident, if an accident driver complies with the speed limit and other traffic laws and it is impossible to avoid the accident, the driver should not be punished. So, in order to meet the principle of proportionality, the new Korean law should be revised again. In order to find out the appropriate level and punishment method for drivers who cause accidents in school zones, this thesis will compare and analyze the laws of Korea with those of the United States, Germany, and Japan. This paper also reviews the decision of the Constitutional Court of the Republic of Korea in February 2023 that the "Minsik Law" was constitutional. Based on these analyses, this thesis seeks the direction and amendments to properly revise Korean law. In addition, this thesis is intended to present exemplary measures to improve the school zone safety.

• Korean Journal of Soil Science and Fertilizer
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• v.42 no.6
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• pp.460-466
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• 2009

Carbon fixation and density of crops are important to estimate carbon uptake or emission by agricultural production activities and to establish life cycle inventory of crops for assessment of climate change impact. In this study, regional carbon fixation and density in each part of 14 crops, harvest index, and ratio of aboveground to underground were investigated to estimate biomass of 14 crops in Korea by using agricultural statistics data. Biomass yield of potato was $16.5ton\;ha^{-1}$, which was the highest, and those of rice, sweet potato, and garlic were $10.5ton\;ha^{-1}$, $8.7ton\;ha^{-1}$, and $7.5ton\;ha^{-1}$ respectively. Biomass yield of Green onion was the lowest as $2.8ton\;ha^{-1}$. Carbon density of 14 crops were in the order of potato ($6.4ton\;ha^{-1}$), rice ($4.2ton\;ha^{-1}$), sweet potato ($3.4ton\;ha^{-1}$), rape ($2.9ton\;ha^{-1}$) and garlic ($2.8ton\;ha^{-1}$). Regional distribution of carbon contents for each crop mapped revealed that carbon fixation of rice, soybean, sesame, garlic, and green onion were the highest in Jeonnam province, barley, red pepper, and watermelon in Gyeongnam, perilla in Chungnam, peanut in Gyeongbuk, rape and carrot in Jeju, sweet potato in Gyeonggi, potato in Gangwon. The results can be applied for assessing life cycle inventory of crops and crop productivity using remotely sensed data.

• Journal of Food Hygiene and Safety
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• v.28 no.2
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• pp.130-137
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• 2013

In this study, we investigated the applicability of the photostimulated luminescence (PSL), thermoluminescence (TL), electron spin resonance (ESR) and gas chromatography/mass spectrometry (GC/MS) methods for 5 seeds which are not allowed to be irradiated in Korea. All 5 seeds including evening primrose seed, safflower seed, rape seed, sunflower seed and flax seed were analyzed. Samples were irradiated at 1~10 kGy using a $^{60}Co$ gamma-ray irradiator. In PSL study, the photon counts of all the unirradiated samples showed negative (lower than 700). The photon counts of irradiated (1, 5, 10 kGy) samples showed positive (higher than 5,000). In TL analysis, results showed that it is possible to apply TL method to all foods containing minerals. In ESR measurements, the ESR signal (single-line) intensity of irradiated foods was higher than non-irradiated foods. The hydrocarbons 1,7-hexadecadiene ($C_{16:2}$) and 8-heptadecene ($C_{17:1}$) from oleic acid were detected only in the irradiated samples before and after the treatment at doses ${\geq}$ 1 kGy, but they were not detected in non-irradiated samples before and after treatment. These two hydrocarbons could be used as markers to identify irradiated safflower seed, rape seed, Sunflower seed and flax seed. And then, the hydrocarbons 1,7,10-hexadecatriene ($C_{16:3}$) and 6,9-heptadecadiene ($C_{17:2}$) from linoleic acid were detected in the evening primrose seed, safflower seed and sunflower seed. According to the results, PSL, TL and GC/MS methods were successfully applied to detect the irradiated foods. It is concluded that PSL, TL and GC/MS methods are suitable for detection of irradiated samples and a combined method is recommendable for enhancing the reliability of detection results.

• Korean Journal of Food Science and Technology
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• v.5 no.4
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• pp.215-223
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• 1973

A potent citric acid producing strain was selected by an extensive screening test of the yeasts isolated from the various sources. These experiments were conducted to identify the selected strain and investigate the cultural conditions for citric acid production. The results obtained were as fellows: 1. The selected strain of yeast was identified to Hansenula anomala var. anomala by a taxnoomic study of Lodder. 2. The optimum conditions for citric acid production in the basal medium containing 10% glucose were: temperature $30^{\circ}C$, the concentration of $CaCO_3$ 3% and the velocity of oscillation 110 oscills/min. 3. As a nitrogen source ol the basal medium $NH_4Cl(0.1%)$ was the most effective for citric acid production. Organic nitrogen sources such as peptone were adequate for growth of the strain but not for citric acid production. 4. The most effective concentration of glucose was 10% in yield ratio of citric acid from sugar. 5. The addition of defatted rape seed, defatted perilla or defatted rice bran to the medium was effective for citric acid production. When 5% extract solution of defatted rape seed was added, the citric acid production was increased as much as 40% as compared with the case of adding yeast extract(0.2%). 6. The most effective concentration of $KH_2PO_4$ and $MgSO_4{\cdot}7H_2O$ in the medium(for citric acid Production) was 0.05% and 0.025% respectively. 7. Under the optimum cultural conditions, the growth of the strain was continued up to 5 days and the increase of citric acid production was continued up to 6 days, showing the yield ratio of 46% to glucose.

• The Korean Journal of Mycology
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• v.38 no.2
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• pp.130-135
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• 2010

To select the viable alternative substrates among the variable organic substrates for productivity enhancement and production cost-reduction of oyster mushroom in bottle culture, this study was carried out at mushroom research institute of GGRDA in 2007. In bottle culture of oyster mushroom (Plerutus ostreatus), the seedcakes of rape (RS), soybean (SS), coconut (CCS), and kapok (KS) were examined as substitute of cotton seedcake which was primary nutritive material of mushroom growing substrate. The chemical properties of substrate mixed with kapok seedcake is similar to the mixture with cotton seedcake in T-C, T-N, C/N ratio, and other nutrients. Mixed growing substrate containing cotton seedcake and kapok seedcake was superior to other mixtures 99.2% and 99.5%, respectively in spawning ratio and was faster mycellium growth in column test than that of soybeen seedcake, cotton + soybeen seedcake, and coconut seedcake. The period required in first pin-heading was 1-2 days longer in rape and soybeen seedcake mixture. Also there wad no primodia and fruitbody formation at soybeen seedcake mixture which had highest T-N content among the other mixed substrates. Yield per bottle and biological efficiency were highest of 144.6 g and 75.4%, respectively at kapok seedcake mixture. As a result, this study found that cotton seedcake can be replaced with kapok seedcake in bottle culture of oyster mushroom.