• Korean Journal of Veterinary Research
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• 제32권3호
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• pp.381-387
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• 1992

Gill epithelia of normal rainbow trout fingerlings and abnormal ones suffering bacterial gill disease by experimental infection were examined by transmitting electron microscopy (TEM) and scanning electron microscopy (SEM). TEM observations revealed that Flavobacterium branchiophila consisted of slender rods measuring 0.5 by 5 to $8{\mu}m$, and they had which were long, thin, flexible filaments measuring approximately 4 nm by $1{\mu}m$, and packed together to organize into bundles. Morphological alterations of the diseased epithelia started at hypertrophy of the lamellar epithelium. F branchiophila attached to the gill surface of infected fish through pili with a regular distance, and did not invade into gill tissue. In SEM observations, normal surface ultrastructure of epithelial cell in the outermost layer were characterized by a typical labyrinth-like structure branching and anastomosing microridges on the cell surface. Hyperplastic lesions in experimentally infected gill were most serious at near the tips. Each filament exhibited a club-like, and fusion between the filaments was sometimes observed at their tips. On the surface of gill filaments, thread-like bacterial cells attached and were entangled. The bacterial cells almost covered the surface. After immersion in 5 % NaCl, the cell of F branchiophila, however, appeared to be indeterminate shape.

• Korean Journal of Fisheries and Aquatic Sciences
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• 제27권3호
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• pp.240-246
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• 1994

This study was designed to determine the optimum pigment concentration supplemented in diet and feeding periods on the pigmentation of rainbow trout(Oncorhynchus mykiss) by using acetone-extracts of ascidian tunic as a natural pigment source. The eight pigmented diets contained carotenoid of ascidian tunic extracts at concentrations of 0, 200, 400, 800, 1,600 and 3,200mg/kg of diet, carophyll pink at concentration of 800mg/kg and commercial diet. No difference in pigment concentration was found between the ascidian extracts group and the control group until 4 weeks, but the redness of muscle and integument in the 1,600, 3,200mg/kg diet and carophyll pink was increased in the dorsal and caudal areas of fish from 6 weeks of age. In the sensory panel test, fish fed the ascidian tunic extracts diet were similar to those fed the carophyll pink diet. The optimum concentration and feeding periods for pigmentation of rainbow trout was found to be ascidian tunic extracts of 1,600mg/kg diet for 8 weeks.

• Horticultural Science & Technology
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• 제29권6호
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• pp.651-654
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• 2011

Cymbidium 'Orange Bowl' (Lucky Rainbow 'Randevous' ${\times}$ 'Eastern Star') was developed from a cross between hybrids at the National Institute of Horticultural & Herbal Science, Rural Development Administration in 2006. A cross was made between the pink colored flower C. Lucky Rainbow 'Randevous' as maternal line and pure yellow colored flower, C. 'Eastern Star' as paternal line in 1995. The seed germination, cultivation, selection, and characteristic trials were conducted from 1996 to 2006. The line was named as Wongyo F1-18 and phenotype was characterized in 2006 as a new 'Orange Bowl'. The 'Orange Bowl' has having light yellow basal color (RHS, YO21D) and orange line (RHS, OR30B) on both of sepal and petal with red lip (RHS, OR30B). 'Orange Bowl' has about 10.9 flowers per flower stalk and flower size of 7.4 cm. General appearance of the petals and sepals is slightly incurved shape. The plant size is intermediate having erect peduncle. Blooming is started from the late of January (mid winter) under optimal culture condition. Leaf attitude and twisting is half- erect and very weak respectively. This hybrid has attractive floral arrangement, long flower stalk (71.8 cm) and vigorous growth. We expect that 'Orange bowl' has a great potential for exporting to Chinese market.

• Journal of Aquaculture
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• 제11권3호
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• pp.311-317
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• 1998

Effects of Cu and Zn on estradiol-17$\beta$-Induced vitellogenin (VTG) production were electro-phoretically examined in hepatocyte cultures of rainbow trout. Hepatocytes were precultured for 2 days and then Cu ($10^{-5}$ ~$10^{-4}$M) and Zn ($10^{-5}$~$10^{-3}$M) were added to the incubation medium with estradiol-17${\beta}$ ($2{\times}10^{-6}$M). The hepatocytes were cultured for 5 more days. The relative VTG production rate was expressed as the percentage of VTG to total proteins including the VTG. The addition of CU and Zn to the incubation medium had no appreciable toxin effect on the viability of hepatocytes in the culture. However, Cu markedly reduced VTG production at any concentration used. Zn also specifically reduced VTG production by hepatocytes in a concentration dependent way and there was a significnt reduction at Zn concentrations of $10^{-3}$M. The reduction recovered by removing Zn from the media, but Cu did not. Additionally, enriched Ca concentrations (1.8 to 2.5 or 5.0 mM) in the incubation medium had no protective effect on the reduction of VTG production by Cu $10^{-4}$ M. These results suggest that the production of VTG is more susceptible to Cu and Zn than are other hepatocyte-derived proteins.

• Korean Journal of Ichthyology
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• 제24권2호
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• pp.118-124
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• 2012

The present study was conducted to investigate different gene expression profile between treated poMSTNpro and non-treated in rainbow trout and to identify those genes that are specifically or predominantly expressed in treated poMSTNpro by employing annealing control primer (ACP)-based GeneFishing polymerase chain reaction (PCR). We isolated total RNAs in muscle tissues from the treated poMSTNpro fish by immersion bath technique with fish myostatin prodomain (Paralichthys olivaceus, poMSTNpro) for one month and the other was non-treated poMSTNpro, and synthesized cDNA using annealing control primers (ACP, Seegene, Korea). Using 20 different ACPs for PCR, were cloned sequenced, and analyzed identities of 2 differentially expressed genes (DEGs). According to BLAST analysis, sequences of 2 clones significantly matched database entries and confirmed by semi-quantitative RT-PCR. The functional roles of one up-regulated gene, cytochrome P450 mono-oxygenases 2K1v2 (CYP2K1v2), and one down-regulated gene was Profilin-1 were identified. We identified distinctive gene expression profiles in improved rainbow trout growth by treatment with a fish myostatin prodomain using ACP-based GeneFishing.

• Journal of Aquaculture
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• 제10권3호
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• pp.347-356
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• 1997

Feeding experiment was conducted to evaluate effects of dietary soybean meal (SBM) on the phosphorus bioavailability of rainbow trout (Oncorhynchus mykiss). Seven diets, containing 0~70% SBM, were made based on iso-nitrogenous and iso-caloric basis of 44% crude protein. Ajpparent digestibility of phosphorus was measured 60 and 120 days after feeding. The digestibility coefficient increased gradually as the level of SBM increased up to 46~58%, but decreased at 58~70% SBM level. Phosphorus concentrations in feces decreased with an increased in dietary SBM level. Patterns of post-prandial phosphorus excretion were not similar to those of ammonia excretion, but the amount decreased with the increased SBM level in the diets. Total excretion of phosphorus for 24 hours was 19.68mg.kg-1 body wt..day-1 in the control group, but excreted phosphorus by the 58% and 70% SBM groups was 63.4% and 56.1% of the control group, respectively. Phosphorus concentration of whole body decreased with an increase in dietary SBM level for 120 days of feeding. However, there were no significant differences in phosphorus concentrations of liver among the experimental groups, except for 70% SBM diet group.

• Current Research on Agriculture and Life Sciences
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• 제31권1호
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• pp.56-60
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• 2013

This research was carried out to produce rainbow-colored roses from white cut rose, Rosa hybrida 'Denice', to meet customers' desire of having various colors of roses. The higher temperature of dyeing solution used, the faster coloring time surveyed. In high temperature, the flowering speed of cut flower accelerated and after treating more than 4 hours, the edges of flowers became too dark or dry. In the condition of concentration of dyeing solution, $11g{\cdot}L^{-1}$ dyed faster than $7.5g{\cdot}L^{-1}$, however, no differences between $15g{\cdot}L^{-1}$ and $11g{\cdot}L^{-1}$ have observed. It looked fast coloration at the stage of early blooming, but all three different petal opening stages need similar time to get even coloration from outer to inner petals. For the consideration of commercial value, flowers with 3-4 petals opened are better quality than flowers with less petals opened. It was possible to make rainbow-colored rose by dipping 'Denice' with 3 primary color combination, Hot Pink, True Blue, and Yellow dyes, at the best result conditions of $20^{\circ}C$ solution temperature, 3-4 petals opening stage, 11g/L concentration of dye solution and 3 hours dipping, respectively.

• Journal of fish pathology
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• 제7권1호
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• pp.63-70
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• 1994

Nitric oxide(NO) has recently been shown to play an important role on central nervous system(CNS) function in mammals. It is synthesized from L-arginine by the enzyme NO synthase. In this study, we examined this enzyme's existence in CNS of rainbow trout(Oncorhynchus mykiss) and described the forms of microglia. astrocytes. and oligodendrocytes. Two forms of microglia are distributed in CNS. one resembling their mammalian counterpart(large microglia : LM). and the other comprising small microglia(SM) with very little cytoplasm. CNS contained astrocytes of a distinct type which form reticular network, but lack connections to capillaries. The oligodendrocyte was generally a much denser cell than the astrocyte. We have detected NOS($1.04{\pm}0.12\;pg/min/mg$) from rainbow trout CNS. It could be inhibited reversibly or irreversibly by $N^{G}MMA$ and EGTA. These result suggest that the formation of NO from L-arginine in CNS is calcium-dependent and a pathway of early evolutionary orgin.

• Journal of Hospice and Palliative Care
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• 제12권4호
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• pp.209-219
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• 2009

Purpose: This study was to testify the effects on self esteem and spiritual well-being of holistic hospice nursing intervention program ("Rainbow program") for inpatients of hospice palliative care unit. This was designed as a preliminary experimental study with one-group pre-post test. Methods: A total of 27 patients who were over 18 years old, and admitted in hospice palliative care unit of S hospital in P city, submitted informed consent for this study, participated in holistic hospice nursing intervention program(total 10 sessions and 1,200 minutes for 2 weeks) from April 6, 2004 to April 20, 2005. To test the effects of this intervention, Self Esteem Questionnaire (SEQ) and Spiritual Well-being Questionnaire were used. The collected data were analyzed by Paired t-test with SPSS/WIN 12.0 program. Results: (1) Hypothesis No. 1 "The experimental group which received Rainbow program will have a higher degree of self esteem than before" was supported (t=11.554, P<0.001). (2) Hypothesis No. 2 "The experimental group which received Rainbow program will have a higher degree of spiritual well-being than before" was also supported (t=6.387, P<0.001). Conclusion: This Holistic Hospice Nursing Intervention Program was effective in increasing self-esteem and spiritual well-being of patients in hospice palliative care unit. Therefore, it can actively be used and also applied to hospice palliative care practice, research, and education as a useful model of interdisciplinary team approach by hospice professionals.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1997년도 한국자동제어학술회의논문집; 한국전력공사 서울연수원; 17-18 Oct. 1997
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• pp.1880-1883
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• 1997

An Parallax-Barrier type autostereoscopic display system is implemented to overcome the handicap of current glass-typed stereo imaging system which gives operators unnatural feeling and reluctance. This system is composed of image mixing part of two input images, A/D transformation part, scan conversion part to eliminate the rainbow interference phenomenon, and pin-hole type barrier lens. For the fine display the accurate slit width of barrier is calculated in consideration of retina size, and the samsung 10.4" TFT-LCO was used as flat display monitor.itor.