• 한국수산과학회지
• /
• 제55권4호
• /
• pp.417-424
• /
• 2022

To develop a value-added anchovy Engraulis japonicus sauce, the processing conditions and quality characteristics of a rapidly fermented and high-purity anchovy sauce (RRAS) were examined by adding 5-10% (w/w) radish Raphanus sativus L. RRAS exhibited higher yield, total nitrogen content, and amino nitrogen and calcium contents as well as lower salinity than those of conventional anchovy sauce (control). The salinity, yield, and total amino acid contents of RRAS and control were 17.2-17.7% and 19.6%, 81.2-88.7% and 61.0%, and 13,117.8-14,174.9 mg/100 g and 10,041.1 mg/100 g, respectively. The major amino acids recorded were aspartic acid, glutamic acid, alanine, valine, isoleucine, leucine, phenylalanine, ornithine, lysine, and histidine. The histamine contents of RRAS and control were 8.3-8.6 mg/100 g and 19.2 mg/100 g, respectively, while the protease activity levels were 0.901-0.958 unit/mg and 0.695 unit/mg, respectively. Overall, this study establishes that RRAS can not only significantly shorten the salt fermentation period, but can also serve as an anchovy sauce with superior nutritional quality and higher levels of amino acid and calcium.

• 한국동물학회:학술대회논문집
• /
• 한국동물학회 1998년도 한국생물과학협회 학술발표대회
• /
• pp.317.2-317
• /
• 1998

No Abstract, See Full Text

• 한국동물학회:학술대회논문집
• /
• 한국동물학회 2000년도 한국생물과학협회 학술발표대회
• /
• pp.221.2-221
• /
• 2000

No Abstract, See Full Text

• BMB Reports
• /
• 제31권6호
• /
• pp.548-553
• /
• 1998

A minor anionic isoperoxidase named $A_{3n}$, was isolated from Korean radish (Raphanus sativus L.) root. Purification of the enzyme was accomplished by CMcellulose chromatography, DEAE-Sephacel chromatography, and Sephadex G-75 gel filtration. The enzyme was a glycoprotein with molecular weight of approximately 31,000 as determined by SDS-PAGE and 33,000 by Sepadex G-150 gel filtration, which is by far the smallest among the reported isoperoxidases. The pI value was 3.5. The optimum pH of the enzyme was 6.5 for guaiacol and $H_2O_2$, and the $K_m$ values for guaiacol and $H_2O_2$ were 13.3 mM and 1.5 mM, respectively. Kinetic studies with various substrates revealed that only A3n, unlike other isoperoxidases from radish, did not use scopoletin as a substrate and had very low $K_m$ value of 0.25 mM for ferolic acid among naturally occurring phenolic substrates.

• 한국식품저장유통학회지
• /
• 제20권1호
• /
• pp.104-110
• /
• 2013

무시레기와 신선초의 항산화 성분 및 활성을 분석하여 비교하였다. 무시레기와 신선초 추출물의 polyphenol 함량은 각각 100 g당 $2.84{\pm}0.11g$, $2.88{\pm}0.02g$이었으며, flavonoid 함량은 $0.19{\pm}0.00g$, $0.38{\pm}0.01g$ 으로 확인되었다. 항산화 활성 측정 결과, 무시레기 및 신선초 추출물이 DPPH radical 소거활성에서 $IC_{50}$값이 $348.72{\pm}31.65{\mu}g/mL$, $183.37{\pm}48.17{\mu}g/mL$으로 나타났으며, $ABTS^+$ radical 소거활성은 17.8 %, 11.9 %의 활성을, SOD 유사활성에서는 63%, 58%의 활성을 가진 것으로 나타났다. 총 polyphenol 및 flavonoid 함량과 항산화 활성간의 상관관계에서 총 flavonoid 함량과 DPPH radical 소거능이 r=0.9804, $ABTS^+$ radical 소거활성은 r=-0.9863의 상관관계를 보였으며, DPPH radical 소거활성은 $ABTS^+$ radical 소거활성과 r=-0.9609, SOD 유사활성과는 r=0.4865로서 각각 유의한 부와 정의 상관관계를 나타내었다.

• 한국유기농업학회지
• /
• 제9권3호
• /
• pp.103-117
• /
• 2001

This study was inducted to investigate the effects of microbial fertilizers on the fields of young radish(Raphanus sativus L.), chemical components of plant and soil, and the microbial floras. Six microbial fertilizers, MPK+Husk+Palma, Husk+Palma, MPK+Compost, Compost, BIO Livestock Clean System(BLCS) cattle dropping, and Tomi were used. The yields of young radish were increased in six microbial fertilizer treatments. The fresh matter weight, the number of leaves, and the dry matter weight of young radish in BLCS cattle dropping treatment, the leaf length in MPK+Husk+Palma treatment, the sugar content in Husk+Palma treatment, arid the leaf width in Tomi treatment showed the highest amount, respectively. The effects of microbial fertilizers on chemical characteristics of young radish and soil were examined. Phosphoric acid amount of young radish in Tomi treatment was much higher than other treatments. Potassium amount of young radish showed high significance in all microbial fertilizer treatments compared with control, and shoved the highest in Compost treatment . Two components, phosphoric acid find potassium, in soil inoculated by microbial fertilizers showed significant. Phosphoric acid in the Tomi treatment and Potassium in Husk+Palma treatment were increased. The microorganic populations in soil inoculated with microbial fertilizers were examined. While the number of Bacillus in ceil was increased in MPK+Husk+Palma treatment, the numbers of total bacteria, actinomycetes, and fungi were increased in Tomi treatment.

• 생물환경조절학회지
• /
• 제5권2호
• /
• pp.236-247
• /
• 1996

본 실험은 양액의 종류와 배지의 종류, 그리고 양액의 농도가 20일 무의 생육과 몇가지 품질에 미치는 영향을 알아보고자 실시하였다. 20일 무의 양액재배시 적절한 양액의 구명을 위하여 기존의 Cooper, Hoagland, Yamazaki양액의 비교실험을 실시한 결과, YamaBaki양액이 엽장, 엽수, 지상부와 지하부 생체중 등 전반적으로 Hoagland와 Cooper 양액보다 좋은 결과를 나타내었다. 근형지수는 모든 처리구에서 약 0.66으로 비교적 낮은 수치를 보였다. 14가지 조합의 배지를 이용하여 고형배지경을 실시하였다. 그 결과 모래 배지가 가장 균일하고도 좋은 생육의 20일 무를 배출하였고, 피트모스는 반대의 생육을 보였다. 근형지수의 경우 전반적으로 고형배지경이 순수수경재배보다 높은 수치를 나타내었다. Yamazaki 양액의 적정농도 수준을 구명을 위하여 모래를 배지로 하여 20일 무의 생육과 품질을 비교 조사한 결과, 1.0mS/cm 이상의 농도에서는 전반적으로 20일 무의 생육이 좋았으며, 특히 1.5mS/cm가 가장 좋은 생육을 보였다. 비타민 C의 함량과 티오시아네이트 함량은 1.0mS/cm이상의 처리구에서는 현저한 차이가 없었으나, 0.5mS/cm는 상당히 높은 수치를 보였다. 또한 무기물 함량의 경우는 모든 처리구에서 통계적 유의성이 없었으나, 칼륨이 1.5mS/cm에서 비교적 높은 함량을 나타내었다.

• Molecules and Cells
• /
• 제24권1호
• /
• pp.37-44
• /
• 2007

Three catalase cDNA clones were isolated from the small radish (Raphanus sativus L.). Their nucleotide and deduced amino acid sequences showed the greatest homology to those of Arabidopsis. Genomic Southern blot analysis, using RsCat1 cDNA as a probe, showed that catalases are encoded by small multigene family in the small radish. Nondenaturing polyacrylamide gels revealed the presence of several catalase isozymes, the levels of which varied among the organs examined. The isozyme activities were assigned the individual catalase genes by Northern analysis using total RNA from different organs. The three catalase genes were differentially expressed in response to treatments such as white light, xenobiotics, osmoticum, and UV. Their expression in seedlings was controlled by the circadian clock under a light/dark cycle and/or in constant light. Interestingly, RsCat1 transcripts peaked in the morning, while those of RsCat2 and RsCat3 peaked in the early evening. Our results suggest that the RsCat enzymes are involved in defense against the oxidative stress induced by environmental changes.

• 한국작물학회지
• /
• 제54권4호
• /
• pp.346-350
• /
• 2009

The cultivated radish (Raphanus sativus L.) is a major vegetable crop in the world wide and fast-growing species that grows inhabitats of six continents. It is very important to determine hybrid seed purity in the production of hybrid Brassica vegetable seeds to avoid unacceptable contamination with self-inbred (sib) seeds. The use of random amplified polymorphic DNA (RAPD) markers for evaluating seed purity in $F_2$-hybrid radish cultivars demonstrated. One hundred eighty seeds from the F1 male and female harvest were subsequently screened for seed purity using 13 primers. The 13 primers result in 17 cultivar-specific bands and 23 variable RAPD bands scored for cultivar. RAPD analysis of hybrid seeds from the harvest revealed 128 seeds tested except underdevelopment and decayed seeds were sibs. Especially, $F_2$ hybrids of radish, OPC13, OPD20 were presented clear hybrid bands. It maintains higher than average level of genetic diversity compared with their correspondent parents. RAPD amplification of DNA extracted from germinated individuals from the female harvest reveal that 10 of 208 seeds tested were self-inbred (4.8%). RAPD analysis of hybrid seeds from the male harvest revealed 7 of the 208 seeds tested were sibs (3.4%). The RAPD may lead to a better insight in to the hybrid seed purity.

• 한국동물학회:학술대회논문집
• /
• 한국동물학회 1999년도 한국생물과학협회 학술발표대회
• /
• pp.220.2-220
• /
• 1999

No Abstract, See Full Text