Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Antioxidant Activities of Angelica keiskei L. and dried leaves of Raphanus sativus L.

신선초와 무시레기의 항산화 성분 및 활성 비교

  • Ji, Su-Jin (Functional Food & Nutrition Division, Department of Agrofood Resoures, National Academy of Agricultural Sciences, Rural Development Administration) ;
  • Lee, Dong-Jin (College of Bio-Resources Sciences, Dankook University) ;
  • Lim, Sung-Hee (Functional Food & Nutrition Division, Department of Agrofood Resoures, National Academy of Agricultural Sciences, Rural Development Administration) ;
  • Shin, Woo-Jeong (Functional Food & Nutrition Division, Department of Agrofood Resoures, National Academy of Agricultural Sciences, Rural Development Administration) ;
  • Cho, Young-Suk (Functional Food & Nutrition Division, Department of Agrofood Resoures, National Academy of Agricultural Sciences, Rural Development Administration) ;
  • Kim, So-Young (Functional Food & Nutrition Division, Department of Agrofood Resoures, National Academy of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Jung-Bong (Functional Food & Nutrition Division, Department of Agrofood Resoures, National Academy of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Se-Na (Functional Food & Nutrition Division, Department of Agrofood Resoures, National Academy of Agricultural Sciences, Rural Development Administration)
  • 지수진 (농촌진흥청 국립농업과학원 농식품자원부 기능성식품과) ;
  • 이동진 (단국대학교 생명자원과학대학) ;
  • 임성희 (농촌진흥청 국립농업과학원 농식품자원부 기능성식품과) ;
  • 신우정 (농촌진흥청 국립농업과학원 농식품자원부 기능성식품과) ;
  • 조영숙 (농촌진흥청 국립농업과학원 농식품자원부 기능성식품과) ;
  • 김소영 (농촌진흥청 국립농업과학원 농식품자원부 기능성식품과) ;
  • 김정봉 (농촌진흥청 국립농업과학원 농식품자원부 기능성식품과) ;
  • 김세나 (농촌진흥청 국립농업과학원 농식품자원부 기능성식품과)
  • Received : 2012.11.02
  • Accepted : 2012.12.28
  • Published : 2013.02.28
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Abstract

Dried leaves of Raphanus sativus L. and Angelica keiskei L. extracts have strong antioxidant potential and in consequence profound effects on ulcerative colitis. Present study was conducted to explore the effect of diet mixtures containing dried leaves of Raphanus sativus and Angelica keiskei powder on ulcerative colitis in mice and antioxidant potential of radish green and Angelica extracts as well. Both dried leaves of Raphanus sativus and Angelica keiskei extracts exhibited higher antioxidant activity due to the presence of polyphenols, favonoids and antioxidant enzymes like superoxide dismutase (SOD). Analyzed data indicate that Angelica keiskei extract had higher polyphenol and flavonoid contents compared with radish green, while maximum SOD activity was noted in dried leaves of Raphanus sativus extract Likewise, higher antioxidant ($348.72{\pm}31.65{\mu}g/mL$) and $ABTS^+$ radical scavenging (17%) activities were noted in dried leaves of Raphanus sativus extract compared with Angelica keiskei.

무시레기와 신선초의 항산화 성분 및 활성을 분석하여 비교하였다. 무시레기와 신선초 추출물의 polyphenol 함량은 각각 100 g당 $2.84{\pm}0.11g$, $2.88{\pm}0.02g$이었으며, flavonoid 함량은 $0.19{\pm}0.00g$, $0.38{\pm}0.01g$ 으로 확인되었다. 항산화 활성 측정 결과, 무시레기 및 신선초 추출물이 DPPH radical 소거활성에서 $IC_{50}$값이 $348.72{\pm}31.65{\mu}g/mL$, $183.37{\pm}48.17{\mu}g/mL$으로 나타났으며, $ABTS^+$ radical 소거활성은 17.8 %, 11.9 %의 활성을, SOD 유사활성에서는 63%, 58%의 활성을 가진 것으로 나타났다. 총 polyphenol 및 flavonoid 함량과 항산화 활성간의 상관관계에서 총 flavonoid 함량과 DPPH radical 소거능이 r=0.9804, $ABTS^+$ radical 소거활성은 r=-0.9863의 상관관계를 보였으며, DPPH radical 소거활성은 $ABTS^+$ radical 소거활성과 r=-0.9609, SOD 유사활성과는 r=0.4865로서 각각 유의한 부와 정의 상관관계를 나타내었다.

Keywords

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