• 대한본초학회지
• /
• 제31권6호
• /
• pp.21-28
• /
• 2016

Objectives : This study was performed to investigate the antioxidant activity of water extracts from Lycopus lucidus Turcz. ex Benth. leaves, stems and roots at the $100{\mu}g/m{\ell}$ concentration. Methods : The different part of Lycopus lucidus Turcz. ex Benth. extract was prepared using water. The antioxidant activities of polyphenol contents, total flavonoid contents, DPPH(1,1-diphenyl-2-picrylhydrazyl) free-radical scavenging activity, SOD like activity, hydroxyl radical, ABTS(2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), $Fe^{2+}$ chelating, and nitrite scavenging activity. Results : The total polyphenol and total flavonoid content of leaves were the highest at $221.85{\mu}g/mg$ and $794.13{\mu}g/mg$, respectively. Electron donating ability was the 79.68% in the water extract from leaves. The ABTS radical scavenging activity of the hot extracts, leaves ${\gg}$ roots > stems was higher in the order. It was shown the highest at 94.53% in the water extract from leaves, which showed a value equal to 94.7% of ascorbic acid. The hydroxyl radical scavenging activity was the highest at 8.07% in the water extract from leaves. SOD like activity and $Fe^{2+}$chelating activity were leaves of 12.3% and 27%, respectively, which were much higher than those of any other parts. The nitrite scavenging ability of extracts was increased at pH 2.5, and those was the highest in leaves of 83.03%. Its were more than twice the 41.61% of BHT. Conclusion : The results suggest that Lycopus lucidus Turcz. ex Benth. can be used as nutraceutical foods and natural antioxidant.

• 한국자원식물학회지
• /
• 제26권6호
• /
• pp.686-694
• /
• 2013

Shade treatment of Synurus deltoides (Aiton) Nakai was carried out with 0, 35, and 55% shading net, and samples were marked as no shade, 35% shade, and 55% shade, respectively. We examined in vitro antioxidant and anti-inflammatory capacities using a 1,1-diphenyl-2-2-pricylhydrazyl (DPPH) radical scavenging assay, a reducing power assay, a total antioxidant assay, a metal chelating assay, a superoxide radical scavenging assay, and a nitric oxide inhibition assay. As a result, no shade and 35% shade possessed higher DPPH radical scavenging activity and reducing power ability than that of 55% shade. Notably, no shade had significantly higher total phenolic and flavonoid contents than those in the other samples. No shade exhibited significantly higher total antioxidant activity than that of 35% shade and 55% shade. However, the chelating ability of 55% shade was significantly greater than that of no shade and 35% shade; 55% shade also showed significantly higher anti-inflammatory capacity than that of no shade or 35% shade.

• 한국식품과학회지
• /
• 제32권4호
• /
• pp.928-935
• /
• 2000

오미자 종자의 몇가지 용매 추출물과 분획물의 항산화성, 항균성, 아질산염 소거능에 대하여 확인한 결과 다음과 같다. 돈지와 linoleic acid에 대한 항산화력은 오미자종자의 methanol 추출물이, 대두유는 ethanol 추출물이 가장 효과적이었으며 free radical 소거 활성은 methanol추출물의 ethyl acetate 분획에서 DPPH free radical 소거 활성이 가장 컸다. 오미자 종자 추출 용매별 항균 효과는 L. planterum, B. subtilis, E. coli, P. citrinium는 methanol 추출물이, S. aureus와 S. typhimurium은 ethyl acetate 추출물의 항균 활성이 가장 컸다. 전반적으로 항균 활성이 큰 methanol 추출물을 분획하여 항균력을 검토한 바, buthanol 분획물이 L. planterum, B. subtilis, S. aureus, S. typhimurium 및 E. coli에서 항균 활성이 가장 강하였다. 아질산염 소거 효과는 반응 pH가 낮을수록 우수했으며 methanol 추출물의 buthanol 분획이 다른 추출물과 분획물 중에서 가장 높은 아질산염 분해능을 나타내었다.

• 대한본초학회지
• /
• 제31권6호
• /
• pp.45-52
• /
• 2016

Objectives : This study was performed to investigate the antioxidant activity of water and ethanol extracts from Sasa borealis leaves, stems and roots. Methods : Sasa borealis leaves, stems and roots extract were prepared using water and ethanol. The total polyphenol and total flavonoid contents were analyzed. 2,2-diphenyl-1-picrylhydrazyl(DPPH) radical scavenging activity, SOD like activity, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)(ABTS), hydroxyl radical scavenging activity, and Nitrite scavenging activity assays were carried out to determine the antioxidant activities. Results : The antioxidant activities of the Sasa borealis appeared higher in ethanol extract than water extracts. Total polyphenol and total flavonoids contents in ethanol extracts of leaves were $24.6mg/m{\ell}$ and $14.3mg/m{\ell}$, respectively, which were much higher than those of any other parts. SOD like activity was 70% ethanol extract of the leaves was highest with 15.68%. Electron donating ability was 70% ethanol extract of the leaves had the highest 59.07%. It exhibited high electron donating ability than BHT(45.68%). Nitrite scavenging activity of 70% ethanol extract was higher than the water extract at pH 2.5 and pH 4.2. Nitrite scavenging activity was 70% ethanol extract of the leaves was the highest 75.2%. Hydroxyl radical scavenging activity was 70% ethanol extract of the leaves was highest with 16.16%, showed very low activity than BHT(61.56%). Conclusions : These results suggest that 70% ethanol extracts from Sasa borealis leaves, exhibited higher antioxidant activities than those of root and stem, and can be potentially used as proper natural antioxidants.

• 한국응용과학기술학회지
• /
• 제32권3호
• /
• pp.363-371
• /
• 2015

n-Butanol and ethyl acetate extracts of thermal treatment yam (Dioscorea batatas $D_{ECNE.}$) belonging to the family Dioscoreaceae were measured for their radical scavenging activity and lipid peroxidation inhibition ability. In this study, ethyl acetate extract showed the most potent antioxidant activity evaluated by ferrous ion chelating activity and NO radical scavenging activity. Nevertheless, n-butanol extract was more effective in inhibiting linoleic acid peroxidation. A significant difference between n-butanol extract and ethyl acetate extract in nitrite scavenging activity ${\beta}$-carotene bleaching assays could not be found. Also, the results of this study showed that thermal treatment yam could be used as easily accessible source of natural antioxidants and as a possible food supplement.

• 한국약용작물학회지
• /
• 제27권6호
• /
• pp.427-435
• /
• 2019

Background: A growing interest in health has increased the need for the development of potent antioxidant materials known to play a role in various physiological activities. Currently research and development of non-toxic natural antioxidants with high activity is ongoing. Methods and Results: In this study, we measured 2,2'-azinobis- (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability of 10 plant essential oils, selecting samples of Dendranthema indicum, Dendranthema zawadskii, and Citrus sunki essential oils. The samples were analyzed using liquid chromatography (LC) and the radical scavenging activity on LC-based systems with the same conditions. In the LC-mass spectroscopy (MS)/MS analysis of the active compound peak, 2-methoxy-4-vinylphenol with a molecular weight of 150.1 g/mol was identified in C. sunki essential oils. Eugenol or isoeugenol with a molecular weight of 164.1 g/mol as identified in D. indicum and D. zawadskii essential oils as radical scavenging active compounds. Conclusions: In the LC-based measurement system, the active ingredient can be identified by simultaneously conducting profile analysis and the radical scavenging activity of essential oil samples. In addition, LC-MS/MS analysis of the active compound peaks can be performed under the same separation conditions to obtain data that can identify the active compounds in the sample.

• 한국식품영양과학회지
• /
• 제28권5호
• /
• pp.984-989
• /
• 1999

This study was carried out to investigate the effects of ethanol extracts on lipid oxidation of oil emulsion. The results are as follows; The scavenging ability of plant extracts for hydroxyl radical was found, and plant extracts played an important role as a strong chelating agents to bind iron if Fe2+ ion exists in oil emulsion. Pinus densiflora(PD), Lithospermum erythrorhizon(LE) and PD＋LE acted as strong chelating agents to bind iron to reduce lipid oxidation in oil emulsion. The content of Fe2+ ion in ethanol extracts from LE and PD＋LE were significantly higher(p<0.05) than that of ethanol extracts from PD. The content of total iron has same tendency. The ascorbic acid content of PD(16.36ppm) was slightly higher than those of LE(13.08ppm). Electron donating ability of PD was significantly higher(p<0.05) than those of LE. However, the superoxide(SOD) like ability of LE showed a little higher than those of LE and PD＋LE, which means the strong antioxidant activity of LE. The nitrite scavenging effects were dependent on pH value, however, they decreased as pH value increased. Especially, they almost didn't show the nitrite scavenging effect in pH 6.0. In conclusion, the PD and LE extracts may be used as natural antioxidant sources to reduce lipid oxidation in oil emulsion.

• Preventive Nutrition and Food Science
• /
• 제16권1호
• /
• pp.8-11
• /
• 2011

In this study, we assessed the free radical scavenging and xanthine oxidase inhibitory activities of extracts isolated from the dried roots and stems (including leaves) of Angelica dahurica. The irritation response from these extracts was also assessed to determine potential cosmetic use. Both sources of A. dahurica extracts exhibited radical scavenging properties to different extents. The free radical scavenging potency ($EC_{50}$) of the stems (including leaves) of A. dahurica was 243.33 ${\mu}g/mL$, which is significantly lower (p<0.01) than that observed of the roots (1,161.79 ${\mu}g/mL$). The inhibition values ($EC_{50}$) of xanthine oxidase were as follows: 435.19 ${\mu}g/mL$ (roots) and 434.66 ${\mu}g/mL$ (stems). We noted no significant differences between the two plant parts with regard to ability to inhibit xanthine oxidase activity. After the application of A. dahurica extracts to rabbits for skin and eye irritation tests, no negative effects were observed; therefore, the extracts are considered to be non-irritating to the skin and eye.

• 한국식품영양과학회지
• /
• 제40권3호
• /
• pp.379-384
• /
• 2011

본 연구에서는 김천산 후무사의 일반성분, 유리당, 유기산, 총 페놀 화합물, 플라보노이드 등 화학적 성분을 조사하고 DPPH radical 소거능, superoxide dismutase(SOD) 유사활성, 아질산염 소거능, 환원력 등의 항산화 활성을 평가하였다. 유리당 함량은 fructose>glucose>sucrose>maltose 순이었으며, 유기산 함량은 malic acid가 가장 높았다. 총 페놀 함량과 플라보노이드 함량은 각각 60% acetone 추출물, 80% ethanol 추출물에서 높았다. DPPH radical 소거능, 환원력, 아질산염 소거능은 60% acetone 추출물에서 가장 높은 활성을 보여주었으나, SOD 유사활성은 농도나 추출용매, 온도에 따른 차이가 거의 없었다.

• 한국응용과학기술학회지
• /
• 제41권2호
• /
• pp.386-392
• /
• 2024

COVID-19로 인하여 현대인들은 유해환경 및 다양한 자극에 따른 불안 심리가 높아지게 되면서 천연물질을 선호하게 되었다. 따라서 전통의학에서 무독성 식물로 인정한 히비스커스를 천연화장품 소재로서의 적절성을 알아보고자 히비스커스 추출물의 항산화 효과(폴리페놀, 플라보노이드, DPPH, ABTS)를 연구하여, 다음과 같은 결과를 얻었다. 첫째, 히비스커스의 총 폴리페놀은 HSE 100%일 때 433 ㎍/mL로 나타났다. 둘째, 총 플라보노이드는 HSE 100%에서 488 ㎍/mL로 항산화 능력이 높게 나타났다. 셋째, DPPH radical 소거능은 원액 HES 100%에서 94.04%, 희석액 HSE 20%에서 89.54%로 나타났다. 넷째, ABTS radical 소거능은 HSE 원액 100%에서 98.95%, 희석액 HSE 20%에서 94.84%로 각각 90% 이상의 높은 소거능을 나타냈다. 이러한 연구 결과 히비스커스 추출물은 향후 화장품 천연소재의 항산화 원료로 사용이 가능하다고 사료된다.