• 한국수산과학회지
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• 제50권3호
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• pp.263-269
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• 2017

The mineral contents, and free amino acid, and fatty acid composition of rack and bag or suspended cultured oysters Crassostrea gigas were compared. The moisture content did not change much from 82.8% to 86.3% in the different culture methods. The glycogen level was low 4.7% during rack and bag culture but high 7.2% during suspended culture. The sodium level was the highest during suspended culture, while copper and zinc level were highest during rack and bag culture. The taurine level was 1,385-1,375 mg/100 g in oyster cultured using the rack and bag method, but that in the market sample was lowest at 1,015.8 mg/100 g. Hydroxyproline, glutamic acid, proline, glycine, and alanine were detected at high levels in the experimental oysters. The polyunsaturated fatty acid content was similar among samples. The docosahexaenoic acid level was 14% with rack and bag culture and 12% with suspended culture, whereas the eicosapentaenoic acid level was 15% with rack and bag culture and 20% with suspended culture. The atherogenic index (AI), thrombogenicity index (TI), and hypocholesterolemic/ hypercholesterolemic (h/H) ratio were important factors in the nutritional evaluation. The AI and TI values were 0.5-0.2, and the h/H ratio were 2.0 and 2.4 with the rack and bag and suspended culture, respectively.

• 한국패류학회지
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• 제29권1호
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• pp.43-50
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• 2013

We investigated the growth performance of oysters (initial shell height $57.5{\pm}8.5$ mm) under differing conditions of tidal exposure time and culture rack height in an experiment that commenced in April, 2011. Significant differences were observed in shell height from June 2011, in total weight from August, and in meat weight from September. Fatness tended to decrease during the experimental period, but was not significantly different at the end of the experiment. Significant differences in survival rates were mainly observed from June to August. After September, further changes were not observed in any experimental treatment group. The greatest growth potential ($L_{\infty}$) and survival rate were observed at a sea level of approximately 116 cm. The results indicate that in the study area the use of oyster culture conditions involving 1 or 2 h of tidal exposure and 60 - 70 cm rack height could result in oysters reaching the favored commercial half shell size within 14 months, with > 80% survival.

• 한국패류학회지
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• 제28권2호
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• pp.109-116
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• 2012

In order to understand the effect of introduction of oyster rack culture on natural short-necked clam, Ruditapes philippinarum, stock, we investigated the status of natural clam stock prior to introduction of oyster rack culture at Yeongheung Coast of Wando Island. The growth equation of the clam was estimated as: $L_t=61.46{\cdot}(1-e^{-0.172(t+0.155)})$ from ring radius composition of shell. Instantaneous coefficient of total mortality and natural mortality were calculated as: 2.4087/year and 0.478/year, respectively. The age at first capture was estimated to be 2.55 year. The total biomass was estimated to 3.23 ton in the bed (0.8 ha). Applied by these parameters, the annual recruit biomass and the current yield per recruit (Y/R) was corresponded to $114.7individuals/m^2$ and $92.0g/m^2$, respectively. Maximum sustainable yield (MSY) was estimated 0.77 ton/year which was close to annual catches, 0.74 ton/year, in the area.

• 한국수산과학회지
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• 제50권5호
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• pp.500-505
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• 2017

The nucleotides and their related compounds, including ATP (adenosine triphosphate), ADP (adenosine diphosphate), AMP (adenosine monophosphate), IMP (inosine monophosphate), HxR (inosine) and Hx (hypoxanthine), were nearly identical in oysters Crassostrea gigas from the two culture methods. The K-value was lower than the threshold value such as 11.2-12.1. Although oysters have low amount of IMP, it was detected in this experiment. DPPH radical scavenging activity did not vary significantly with sample amounts (100, 300, and $500{\mu}g/mL$). DPPH (1,1-diphenyl-2-picryl-hydrazyl) radical scavenging activity was 76.0-80.7% compare with the ascorbic acid standard. Superoxide anion scavenging activity reached 49.3% in the rack-and-bag culture sample at $500{\mu}g/mL$. However, the reducing power and $Fe^{2+}$ chelating activity were very low compared with their respective standard. The oyster culture methods did not affect oyster quality in terms of antioxidant activities.

• Fisheries and Aquatic Sciences
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• 제21권4호
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• pp.9.1-9.7
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• 2018

To establish a comprehensive management strategy, as part of the optimization of cultural practice for an oyster rack culture system, we used a numerical model to estimate the primary production in the waters on the eastern coast of Wando island, South Korea. The estimated primary production ranged from 17.12 to $1052.55mgC\;m^{-2}day^{-1}$ ($204.22{\pm}224.75mgC\;m^{-2}day^{-1}$ in average). Except for the times of peak phytoplankton blooms, the estimated primary production (PP) was consistently under $200mgC\;m^{-2}day^{-1}$, which is more similar to the value of PP measured off the western coast of South Korea than the southern coast. No clear relationship was observed between nitrogen content and rainfall with the exception of heavy rainfall events, indicating that precipitation might not be the main source of nutrients in these waters. No clear influence was observed from Doam tidal discharge, located 24 km north from these waters due to main tide comes in this area from the channel between Gunwe-myeon in Wando island and Pukpyeong-myeon in Haenam-gun. Because of the shallow water depth and strong tidal current, resuspension of sediments, which causes an input of nitrogen into the system, could be easily caused by even mild wind and the infrequent passing of ships. Microscopic examination of the phytoplankton composition showed additional contribution of benthic species such as Paralia sulcata into the waters, which increase the productivity of oyster farms in the waters. The availability of nitrate and phosphate for primary production was temporarily limited throughout most of the spring and autumn blooming season.

• 한국패류학회지
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• 제31권2호
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• pp.129-136
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• 2015

본 연구는 완도군 군외면 지역에 수평망식 갯벌참굴 양식을 도입하고자 하는 양식 기술개발의 일환으로서 양식해역의 위생학적 특성을 조사하였다. 해수의 경우 2012년 4월부터 2013년 3월까지 매월 1회 실시하였고, 굴 시료는 높이 90 cm인 가상식 양성망에서 채집하였다. 조사해역의 평균 수온범위와 염분 농도범위는 $8.6-28.0^{\circ}C$이며, 26.2-33.5 psu였다. 해수의 대장균군 및 분변계대장균은 각각 < 1.8-13.0 MPN/100 mL의 범위로, 모두 기준치 43 MPN/100 mL이하를 유지하였다. 한편 굴의 분변계대장균은 기준치를 초과하는 경우는 단 1건에 불과하였다. 강우 발생후, 경과일에 따른 참굴 및 해수의 분변계대장균은 모두 해역평균치로 회복되는 데는 약 5일이 소요되었다. 배수유역의 영향반경은 1-292 m에 불과하여 육상오염원에 의한 영향은 미미하였다. 조사해역은 우리나라 수출용 패류생산해역의 위생학적 수질기준과 미국의 패류양식장에 대한 세균학적 수질기준의 충족하며, 생산된 굴의 경우, 유럽연합 A등급 해역의 위생관리에 충족되는 것으로 확인되었다.

• 생약학회지
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• 제21권3호
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• pp.205-209
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• 1990

The study for the quantitative analysis of saikosaponin of Bupleurum falcatum root produced by tissue culture was attempted. The optimal concentration of 2,4-D was 0.1 mg/l for inducing the callus. The induction and growth of the adventitious root from the callus was obtained in the suspension culture containing MS basal medium supplemented with no plant growth regulators. The results of the quantitative analysis of saikosaponins by high performance liquid chromatography (HPLC) showed that the content of saikosaponin a and c was high in the one-year-old root from somatic embryos and that of saikosaponin d was remarkably high in three-months-old adventitious root from callus.

• 한국패류학회지
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• 제28권1호
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• pp.45-54
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• 2012

한국 서해안 2개 지역에서 수평망식 갯벌굴의 성장과 폐사에 미치는 갯벌 환경요인의 영향을 밝히기 위하여 갯벌굴 양식장의 특성을 조사하였다. 갯벌굴의 양식 적지는 주로 서해안에 산재되어 있으나 지역별 생육환경 및 성장특성은 아직까지 전혀 조사된 바가 없어 양식적지 선정에 어려움을 겪고 있다. 따라서 본 연구에서는 수평망 양식이 이루어지고 있는 충남 태안지역과 서산지역에 대한 갯벌굴의 성장 및 수질, 저질 등에 관한 기초 환경조사를 실시하였다. 하계 및 동계의 기온과 수온, 염분 등은 갯벌굴의 생존에 큰 영향을 미치는 것으로 판단되며, 양식여건은 서산에 비해 태안지역이 좀 더 적합한 것으로 나타났다. 갯벌굴의 성장과 사망률을 측정한 결과 태안지역이 서산지역에 비해 높은 성장률과 낮은 사망률을 보였다. 폐사율 역시 종료 시점인 2011년 6월에 서산지역의 폐사율이 높았다. 수평망식 갯벌굴의 성장은 태안지역이 서산지역 보다 더 높게 나타났다. 갯벌굴 종패의 성장 및 경제성 확보가 가능한 적정 수용밀도는 $50{\times}80cm$ 크기의 플라스틱 양성망의 경우 200개체 내외가 적절한 것으로 여겨진다. 갯벌굴의 양식 적지 선정 및 우량종패의 입식과 적정 밀도로의 선별 분산은 갯벌굴의 성장과 폐사에 크게 영향을 미칠 뿐만 아니라 양식의 성패를 좌우할 수 있는 것으로 나타났다. 한국 정부에서는 2012년부터 갯벌굴을 수산물 10대 고부가가치 전략품목으로 선정하여 친환경 양식 산업으로 육성하고 있다.

• 한국버섯학회지
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• 제4권2호
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• pp.43-47
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• 2006

국내에서 재배하여 생산되고 있는 상황버섯의 일종인 PMO-P4균주에 대한 ITS 영역의 염기서열 분석을 실시하였으며 목질 진흙버섯으로 잘 알려져 있는 P. linteus와 함께 RFLP분석을 통하여 상호 비교한 결과 PMO-P4균주는 P. baumii로 판명되었다. 이 결과를 토대로 이미 보고되어 있는 Phellinus속 균주들과의 종간 ITS 영역의 상동성을 비교한 결과 48.6%-72.2%였으며 본 연구에서 비교한 종들 가운데서는 P. linteus와 상동성이 가장 높았으며 P. gilvus와 상동성이 가장 낮았다.

• The Korean Journal of Physiology and Pharmacology
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• 제9권1호
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• pp.9-15
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• 2005

We attempted to analyze the mechanism of polychlorinated biphenyl (PCB)-induced neurotoxicity and identify the target molecules in the neuronal cells for PCBs.Since the developing neuron is particularly sensitive to PCB-induced neurotoxicity, we isolated cerebellar granule cells derived from 7-day old Sprague Dawley (SD) rats and grew cells in culture for additional 7 days to mimic PND-14 conditions. Only non-coplanar PCBs at a high dose showed a significant increase of total protein kinase C (PKC) activity at phobol 12,13-dibutyrate ([$^3M$]PDBu) binding assay, indicating that non-coplanar PCBs are more neuroactive than coplanar PCBs in neuronal cells. PKC isozymes were immunoblotted with the selected monoclonal antibodies. PKC-${\alpha}$, ${\delta}$, and ε were activated with non-coplanar PCB exposure. Receptor for activated C kinase-1 (RACK-1), anchoring protein for activated PKC, was more induced with exposure to coplanar PCBs than non-coplanar PCBs. Reverse transcription PCR (RT-PCR) analysis showed induction of neurogranin (RC-3) and growth associated protein-43 (GAP-43) mRNA with non-coplanar PCBs. The results indicate that these factors may be useful biomarkers for differentiating non-coplanar PCBs from coplanar PCBs. The present study demonstrated that non-coplanar PCBs are more neuroactive congeners than coplanar PCBs.