• 한국동물학회:학술대회논문집
• /
• 한국동물학회 1998년도 공동 춘계학술대회
• /
• pp.37.1-37
• /
• 1998

No Abstract, See Full Text

• 대한의생명과학회지
• /
• 제9권3호
• /
• pp.139-144
• /
• 2003

Since water borne infection causes acute diseases and results in spread of diseases by secondary infection, the prevention is very important. Therefore, it is necessary to have a method that is rapid and effective to monitor pathogenic bacteria in drinking water. In this study, we employed a systematic method, Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis, to develop an effective monitoring system for possible bacterial contaminants in drinking water. For this purpose, PCR primers were derived from 992 bp region of the 16s rRNA gene that is highly conserved through the different species of prokaryotes. To test whether the PCR primers designed are indeed useful for detecting all the possible microbial contaminants in the water, the primers were used to amplify 16s rRNA regions of different microbial water-borne pathogens such as E. coli, Salmonella, Yersinia, Listeria, and Staphylococcus. As expected, all of tested microorganisms amplified expected size of PCR products indicating designed PCR primers for 16s rRNA indeed can be useful to amplify all different microbial water-borne pathogens in the water. Furthermore, to test whether these 16s rRNA based PCR primers can detect bacterial populations present in the water, water samples taken from diverse sources, such as river, tap, and sewage, were used for amplification. PCR products were for then subjected for cloning into a T-vector to generate a library containing 16s rRNA sequences from various bacteria. With cloned PCR products, RFLP analysis was done using PCR products digested with restriction enzyme such as Hae III to obtain species-specific RFLP profiles. After PCR-RFLP, the bacterial clones which showed the same RFLP profiles were regarded as the same ones, and the clones which showed distinctive RFLP profiles were subsequently subjected for sequence analysis for species identification. By this PCR-RFLP analysis, we were able to reveal diverse populations of bacteria living in water. In brief, in unsterilized natural river water, over 60 different species of bacteria were found. On the other hand, no PCR products were detected in drinking tap-water. The results from this study clearly indicate that the PCR-RFLP-sequence analysis can be a useful method for monitoring diverse, perhaps pathogenic bacteria contaminated in water in a rapid fashion.

• 한국잠사곤충학회지
• /
• 제51권2호
• /
• pp.137-141
• /
• 2013

ITS 1, 2, 5.8S ribosomal RNA gene 염기서열 분석과 주사전자현미경 구조 분석을 통해 3종의 페루산 곤충병원성진균들의 동정을 수행하고자 하였다. 이를 위해 두개의 ITS 부위와 5.8S rRNA gene 부위를 포함하는 PCR product를 증폭하여 염기서열 분석을 수행하였으며 분석된 염기서열을 이용하여 NCBI의 BLAST를 이용하여 가장 높은 상동성을 보이는 종들의 ITS1-5.8S-ITS2 염기서열 정보와 비교분석을 위한 근연종들의 염기서열 정보를 다운로드하여 neighbor joining 분석을 수행하였다. 이를 통해 5.8S rRNA 유전자 염기서열은 속 수준에서도 거의 차이를 보여주지 않을 정도로 매우 안정적으로 보존되어 있음을 확인할 수 있었으며 종간 구분이 모호한 결과를 보여주었다. 그와 반대로 ITS 부위의 염기서열은 종에 매우 특이적임을 확인할 수 있었으며, 비교분석에 사용된 Beauveria bassiana strain 간의 차이는 확인할 수 없었다. ITS 염기서열 분석결과를 뒷받침하고자 곤충병원성 진균류의 동정을 위한 분류 key로 사용되는 미세구조 관찰을 위해 주사전자현미경 관찰과 광학현미경 관찰을 통해 B. bassiana 및 Lecanicillium attenuatum의 전형적 구조를 관찰할 수 있었다.

• 한국동물학회지
• /
• 제37권3호
• /
• pp.343-351
• /
• 1994

The partial nucleotide sequences of 185 ribosomal RNA gene were compared for six cnidarian species (one belongs to class Hvdrozoa: Coryne pusilla. Five belong to class Anthozoa: Beflonella rigida from Octocorallia; Anemonia sulcutu, Anthopfeura kurognne, An thopleura midori from Hexacorallia: Cerianthus filiformis from Ceriantipatharial. The aligned sequence data were used to test the hypothesis on the anthozoan phylosenv by using the distance matrix method and parsimony method. The phvlosenetic inferences resulting from these methods indicate that the anthozoan is a monophvletic group and support the three subclass scheme (Octocorallia, HexBcorallia, Ceriantipatharial within class Anthozoa. The result also indicates that ceriantipatharian is more primitive than the other groups and that family Actiniidae is a monophvletic group within the anthozoan. However, the present analysis does not clearly indicate the phvlogenetic relationships of species among genera.

• 한국응용과학기술학회지
• /
• 제40권4호
• /
• pp.881-889
• /
• 2023

Hymenobacter 속(genus)은 Bacteroidota 문(phylum), Hymenobacteraceae 과(family)의 대표 속(type genus)이다. 이 속에 속하는 세균들은 붉은색 색소를 함유하는 그람 음성 간균으로서, 자연계의 다양한 환경에서 분리되고 있다. 본 연구에서 붉은색 색소를 함유하는 그람 음성 간균이 경남 창원시 소재 창원대학교 교내의 연못에서 분리되었고, 이 세균은 균주 B2로 명명되었다. 균주 B2를 계통분석 및 생화학적으로 분석한 결과, Hymenobacter 속에 속하는 것으로 밝혀졌다. 이 세균의 16S rRNA 유전자 염기서열을 genbank의 BLAST로 분석해 본 결과, 다른 어떠한 세균과도 16S rRNA 유전자 염기서열의 상동성이 새로운 미생물로 인정되는 기준인 98.7%보다 낮은 것으로 나타났다. 균주 B2의 지방산을 분석해 본 결과, 주된 지방산은 summed feature 3(C_16:1 ω7c and/or C_16:1 ω6c, 22.8%), iso-C_15:0(16.2%), anteiso-C_15:0(12.9%), C_16:1ω5c(12.4%) 및 summed feature 4 (iso-C_17:1 I/anteiso-C_17:1)(9.5%)인 것으로 밝혀졌는데, 결과적으로 균주 B2의 지방산 함량은 다른 Hymenobacter 종들의 지방산 함량과 뚜렷한 차이가 있는 것을 알 수 있었다. 이 세균의 16S rRNA 유전자 염기서열은 genbank에 accession number OQ318247로 등록되었다.

• Parasites, Hosts and Diseases
• /
• 제51권4호
• /
• pp.401-412
• /
• 2013

Because of an increased number of Acanthamoeba keratitis (AK) along with associated disease burdens, medical professionals have become more aware of this pathogen in recent years. In this study, by analyzing both the nuclear 18S small subunit ribosomal RNA (18S rRNA) and mitochondrial 16S rRNA gene loci, 27 clinical Acanthamoeba strains that caused AK in Japan were classified into 3 genotypes, T3 (3 strains), T4 (23 strains), and T5 (one strain). Most haplotypes were identical to the reference haplotypes reported from all over the world, and thus no specificity of the haplotype distribution in Japan was found. The T4 sub-genotype analysis using the 16S rRNA gene locus also revealed a clear subconformation within the T4 cluster, and lead to the recognition of a new sub-genotype T4i, in addition to the previously reported sub-genotypes T4a-T4h. Furthermore, 9 out of 23 strains in the T4 genotype were identified to a specific haplotype (AF479533), which seems to be a causal haplotype of AK. While heterozygous nuclear haplotypes were observed from 2 strains, the mitochondrial haplotypes were homozygous as T4 genotype in the both strains, and suggested a possibility of nuclear hybridization (mating reproduction) between different strains in Acanthamoeba. The nuclear 18S rRNA gene and mitochondrial 16S rRNA gene loci of Acanthamoeba spp. possess different unique characteristics usable for the genotyping analyses, and those specific features could contribute to the establishment of molecular taxonomy for the species complex of Acanthamoeba.

• 한국식품영양학회지
• /
• 제19권4호
• /
• pp.496-501
• /
• 2006

본 연구는 대한민국 서해안 갯벌로부터 알칼리성 단백질 분해 효소를 생산하는 세균을 분리하고 분리된 세균으로부터 생산되는 단백질 분해 효소의 생화학적 특징을 조사하는 것이다. 분리 균주는 16S rRNA의 염기 서열, 그람 염색과 전자 현미경사진을 통해 Celidibacter sp. HK-1으로 명명하였다. 분리 균주의 증식과 pretense 생산을 위한 최적 온도는 $25^{\circ}C$이었다. 분리 균주의 증식은 접종 10시간후에 stationary phase에 도달하였다. 효소 생산은 14시간 후 최대 값을 보였다. 효소 활성의 최적 온도와 pH는 각각 $45^{\circ}C$와 pH 9이었다. Pretense의 분자량은 약 50KD이었고 pretense의 부분적인 아미노산 서열은 Ala-Try-Ala-Leu-Asn-Thr-Ser-Val-Thr-Glu-Thr-Phe-Ala-Lys이었다. Protease의 부분적인 아미노산 서열은 Streptomyces avemitilis의 pretense와 높은 상동성을 보였다.

• Plant Biotechnology Reports
• /
• 제5권3호
• /
• pp.289-295
• /
• 2011

The SpoU family of proteins catalyzes the methylation of transfer RNAs (tRNAs) and ribosomal RNAs (rRNAs). We characterized a putative tRNA/rRNA methyltransferase, VaSpoU1 of the SpoU family, from Viscum album (mistletoe). VaSpoU1 and other plant SpoU1s exhibit motifs of the SpoU methylase domain that are conserved with bacterial and yeast SpoU methyltransferases. VaSpoU1 transcripts were detected in the leaves and stems of V. album. VaSpoU1-GFP fusion proteins localized to both chloroplasts and mitochondria in Arabidopsis protoplasts. Sequence analysis similarly predicted that the plant SpoU1 proteins would localize to chloroplasts and mitochondria. Interestingly, mitochondrial localization of VaSpoU1 was inhibited by the deletion of a putative N-terminal presequence in Arabidopsis protoplasts. Therefore, VaSpoU1 may be involved in tRNA and/or rRNA methylation in both chloroplasts and mitochondria.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제8권1호
• /
• pp.51-59
• /
• 2004

Phylogenetic relationships were analyzed among bumblebees using a portion of mitochondrial (mt) 16S ribosomal RNA (16S rRNA). Eight species of true bumblebees and one species of cuckoo bumblebee (Bombini, Apidae), collected from Korea were included in the analysis. Also, one species of true bumblebee imported from several foreign countries for pollination was included. The length of mt 16S rRNA sequence ranged from 496 bp to 508 bp and sequence divergence ranged from 1.4％ (7 bp) to 15.49％ (77bp). As expected, a high A＋T content was observed (78.5％ on average). According to the phylogeny tree derived from parsimony and maximum likelihood analysis, a monphyletic Bombus species, excluding a single cuckoo bumblebee, Psithyrus coreanus, was obtained, but the bootstrap estimate at the node supporting the monophyletic group was very weak (40％ or 46％), suggesting a very close relationship of the cuckoo bumblebee to the true bumblebee. Within Bombus species belonging to identical subgenera subgeneric specific clustering was formed with high bootstrap values, implying validity of the subgeneric names of each species: Pyrobombus for B. ardens and B. modeatus; Megabombus for B. consobrinus wittenburgi and B. koreanus; and Bombus s. str. for B. ignitus, B. hypocrita sapporoensis, and B. terrestris.

• 미생물학회지
• /
• 제53권3호
• /
• pp.216-218
• /
• 2017

생물학적방제 효과가 뛰어난 Bacillus thuringiensis C25 균주의 유전체 분석을 수행하였다. 본 균주는 5,308,062 bp, G+C 비율 35.32%의 염색체와 308,946 bp, 32.23% G+C 함량이 포함된 plasmid를 지닌 것으로 확인되었다. 염색체와 plasmid DNA에 예측된 유전자의 총 수는 5,683개의 단백질 코딩유전자와 107개 tRNA 그리고 42개의 rRNA였다.