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Isolation and Characterization of Gelidibacter sp. HK-1 Producing Alkaline Protease  

Oh, Hyun-Geun (Department of Genomic Engineering, Research Institute of Genetic Engineering, Hankyong National Univesity)
Lee, Soon-Youl (Department of Genomic Engineering, Research Institute of Genetic Engineering, Hankyong National Univesity)
Lee, Jae-Hag (Department of Food and Nutrition, Seoul College)
Publication Information
The Korean Journal of Food And Nutrition / v.19, no.4, 2006 , pp. 496-501 More about this Journal
Abstract
This study was to isolate a bacterium producing a alkaline protease from mud flats of the west seaside of Korea and to investigate the biochemical analysis of the alkaline protease producing from the isolate. The isolate was named as Gelidibacter sp. HK-1 based on 16S rRNA sequence, Gram staining and the photograph of electron microsceope. Optimum temperature for growth and pretense production of the isolate was $25^{\circ}C$. Growth of the isolate was reached at stationary phase after 10hrs followed by inoculation. Maximum activity of protease produced from the isolate was shown after 14hrs. Optimum temperature and pH for the protease activity were $45^{\circ}C$ and pH 9, respectively. Molecular weight of the pretense was about 50KD and the partial amino acid sequence of the pretense was Ala-Try-Ala-Leu-Asn-Thr-Ser-Val-Thr-Glu-Thr-Phe-Ala-Lys. The partial amino acid sequences of the protease showed significant homology with a pretense produced from Streptomyces avermitilis.
Keywords
alkaline protease; mud flat; Gelidibacter; partial amino acid sequence; rRNA sequence;
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