• Steel and Composite Structures
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• 제43권2호
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• pp.271-278
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• 2022

Buckling is one of the most critical phoneme in the design of steel structures. Lateral torsional buckling (LTB) is particularly significant for slender beams generally subjected to loading in plane. The web distortion effects on LTB are not addressed explicitly in standards for flexural design of steel I-section members. Hence, the present study is focused to predict the influence of the web distortion on the elastic (L_r) limiting lengths given in American Institute of Steel Construction (AISC) code for the lateral torsional buckling (LTB) behavior of steel beams due to no provision in the code for consideration of web distortion. For this aim, the W44x335 beam is adopted in the buckling analysis carried out by the ABAQUS finite element (FE) program since it is one of the most critical sections in terms of lateral torsional buckling (LTB). The strain results at mid-height of the web at mid-span of the beam are taken into account as the monitoring parameters. The web strain results are found to be relatively greater than the yield strain value when L/L_r is equal to 1.0. In other words, the ratio of L/L_r is estimated from the numerical analysis to be about 1.5 when the beam reaches its first yielding at mid-span of the beam at mid-height of the section. Due to the effect of web distortion, the elastic limiting length (L_r) from the numerical analysis is obtained to be considered as greater than the calculated length from the code formulation. It is suggested that the formulations of the limiting length proposed in the code can be corrected considering the influence of the web distortion. This correction can be a modification factor or a shape factor that reduces sectional slenderness for the LTB formulation in the code.

• Journal of Microbiology and Biotechnology
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• 제20권3호
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• pp.550-557
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• 2010

Escherichia coli (E. coli) heat-labile enterotoxin B subunit (LTB) was regarded as one of the most powerful mucosal immunoadjuvants eliciting strong immunoresponse to coadministered antigens. In the research, the high-level secretory expression of functional LTB was achieved in P. pastoris through high-density fermentation in a 5-1 fermentor. Meanwhile, the protein was expressed in E. coli by the way of inclusion body, although the gene was cloned from E. coli. Some positive yeast and E. coli transformants were obtained respectively by a series of screenings and identifications. Fusion proteins LTB-6$\times$His could be secreted into the supernatant of the medium after the recombinant P. pastoris was induced by 0.5% (v/v) methanol at $30^{\circ}C$, whereas E. coli transformants expressed target protein in inclusion body after being induced by 1 mM IPTG at $37^{\circ}C$. The expression level increased dramatically to 250-300 mg/l supernatant of fermentation in the former and 80-100 mg/l in the latter. The LTB-6$\times$His were purified to 95% purity by affinity chromatography and characterized by SDS-PAGE and Western blot. Adjuvant activity of target protein was analyzed by binding ability with GMI gangliosides. The MW of LTB-6$\times$His expressed in P. pastoris was greater than that in E. coli, which was equal to the expected 11 kDa, possibly resulted from glycosylation by P. pastoris that would enhance the immunogenicity of co-administered antigens. These data demonstrated that P. pastoris producing heterologous LTB has significant advantages in higher expression level and in adjuvant activity compared with the homologous E. coli system.

• 한국지리정보학회지
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• 제7권4호
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• pp.1-14
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• 2004

본 연구에서는 GIS 응용에서 이동객체의 궤적을 인덱싱하기 위해 기존에 제안되었던 TB(Trajectory-Bundle)-트리의 성능을 개선시킬 수 있는 연결테이블(LinkTable:L-Table) 기반의 확장된 TB-트리(L-Table TB-Tree:LTB-Tree)를 제안하고 아울러 제안하는 색인기법의 성능평가를 위해 다음과 같은 사항을 고려한다. 첫째, 기존의 R*-트리, TB-트리, 그리고 제안하는 LTB-트리를 성능평가 대상으로 선정한다. 둘째, 실험 데이타 집합으로는 랜덤 데이타 집합 및 실제 데이타 집합을 이용한다. 셋째, 시스템의 가용 메모리의 제약을 고려해 메모리 버퍼 크기에 따른 성능평가를 수행한다. 넷째, 다양한 데이타 분포도를 가지고 있는 실험 데이타를 이용하여 성능평가를 수행한다. 마지막으로 삽입성능 및 검색성능(궤적질의 및 영역질의)을 평가한다. 성능평가를 분석한 결과, 제안하는 색인기법이 기존의 색인기법들에 비해 삽입과 궤적질의의 검색 측면에서 더 우수함을 보인다.

• Advances in materials Research
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• 제11권1호
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• pp.59-73
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• 2022

In the construction industry, thin-walled frame elements with very slender open cross-sections and low torsional stiffness are often subjected to a complex loading condition where axial, bending, shear and torsional stresses are present simultaneously. Hence, these often fail in instability even before the yield capacity is reached. One of the most common instability conditions associated with thin-walled structures is Lateral Torsional Buckling (LTB). In this study, a first order Generalized Beam Theory (GBT) formulation and numerical analysis of cold-formed steel lipped channel beams (C80×40×10×1, C90×40×10×1, C100×40×10×1, C80×40×10×1.6, C90×40×10×1.6 and C100×40×10×1.6) subjected to uniform moment is carried out to predict pure Lateral Torsional Buckling (LTB). These results are compared with the Finite Element Analysis of the beams modelled with shell elements using ABAQUS and analytical results based on Euler's buckling formula. The mode wise deformed shape and modal participation factors are obtained for comparison of the responses along with the effect of varying the length of the beam from 2.5 m to 10 m. The deformed shapes of the beam for different modes and GBTUL plots are analyzed for comparative conclusions.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2015년도 학술발표회
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• pp.217-217
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• 2015

The objective of this study was mainly to evaluate the water resources potential of Lake Tana Basin (LTB) by using Soil and Water Assessment Tool (SWAT). From SWAT simulation of LTB, about 5236 km2 area of LTB is gauged watershed and the remaining 9878 km2 area is ungauged watershed. For calibration of model parameters, four gauged stations were considered namely: Gilgel Abay, Gummera, Rib, and Megech. The SWAT-CUP built-in techniques, particle swarm optimization (PSO) and generalized likelihood uncertainty estimation (GLUE) method was used for calibration of model parameters and PSO method were selected for the study based on its performance results in four gauging stations. However the level of sensitivity of flow parameters differ from catchment to catchment, the curve number (CN2) has been found the most sensitive parameters in all gauged catchments. To facilitate the transfer of data from gauged catchments to ungauged catchments, clustering of hydrologic response units (HRUs) were done based on physical similarity measured between gauged and ungauged catchment attributes. From SWAT land use/ soil use/slope reclassification of LTB, a total of 142 HRUs were identified and these HRUs are clustered in to 39 similar hydrologic groups. In order to transfer the optimized model parameters from gauged to ungauged catchments based on these clustered hydrologic groups, this study evaluates three parameter transfer schemes: parameters transfer based on homogeneous regions (PT-I), parameter transfer based on global averaging (PT-II), and parameter transfer by considering Gilgel Abay catchment as a representative catchment (PT-III) since its model performance values are better than the other three gauged catchments. The performance of these parameter transfer approach was evaluated based on values of Nash-Sutcliffe efficiency (NSE) and coefficient of determination (R2). The computed NSE values was found to be 0.71, 0.58, and 0.31 for PT-I, PT-II and PT-III respectively and the computed R2 values was found to be 0.93, 0.82, and 0.95 for PT-I, PT-II, and PT-III respectively. Based on the performance evaluation criteria, PT-I were selected for modelling ungauged catchments by transferring optimized model parameters from gauged catchment. From the model result, yearly average stream flow for all homogeneous regions was found 29.54 m3/s, 112.92 m3/s, and 130.10 m3/s for time period (1989 - 2005) for region-I, region-II, and region-III respectively.

• Steel and Composite Structures
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• 제30권5호
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• pp.483-492
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• 2019

In the current study, the influence of the initial lateral (sweep) shape and the cross-sectional twist imperfection on the lateral torsional buckling (LTB) response of doubly-symmetric steel I-beams was investigated. The material imperfection (residual stress) was not considered. For this objective, standard European IPN 300 beam with different unbraced span was numerically analyzed for three imperfection cases: (i) no sweep and no twist (perfect); (ii) three different shapes of global sweep (half-sine, full-sine and full-parabola between the end supports); and (iii) the combination of three different sweeps with initial sinusoidal twist along the beam. The first comparison was done between the results of numerical analyses (FEM) and both a theoretical solution and the code lateral torsional buckling formulations (EC3 and AISC-LRFD). These results with no imperfection effects were then separately compared with three different shapes of global sweep and the presence of initial twist in these sweep shapes. Besides, the effects of the shapes of initial global sweep and the inclusion of sinusoidal twist on the critical buckling load of the beams were investigated to unveil which parameter was considerably effective on LTB response. The most compatible outcomes for the perfect beams was obtained from the AISC-LRFD formulation; however, the EC-3 formulation estimated the $P_{cr}$ load conservatively. The high difference from the EC-3 formulation was predicted to directly originate from the initial imperfection reduction factor and high safety factor in its formulation. Due to no consideration of geometric imperfection in the AISC-LFRD code solution and the theoretical formulation, the need to develop a practical imperfection reduction factor for AISC-LRFD and theoretical formulation was underlined. Initial imperfections were obtained to be more influential on the buckling load, as the unbraced length of a beam approached to the elastic limit unbraced length ($L_r$). Mode-compatible initial imperfection shapes should be taken into account in the design and analysis stages of the I-beam to properly estimate the geometric imperfection influence on the $P_{cr}$ load. Sweep and sweep-twist imperfections led to 10% and 15% decrease in the $P_{cr}$ load, respectively, thus; well-estimated sweep and twist imperfections should considered in the LTB of doubly-symmetric steel I-beams.

• BMB Reports
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• 제49권4호
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• pp.232-237
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• 2016

Mulberry tree twigs (Ramulus mori) contain large amounts of oxyresveratrols and have traditionally been used as herbal medicines because of their anti-inflammatory properties. However, the signaling mechanism by which R. mori exerts its anti-inflammatory action remains to be elucidated. In this study, we observed that R. mori ethanol extracts (RME) exerted an inhibitory effect on the lipopolysaccharide (LPS)-induced production of the pro-inflammatory cytokine interleukin-6 (IL-6) in Raw264.7 macrophage cells. Additionally, RME inhibited IL-6 production by blocking the leukotriene B₄ receptor-2 (BLT2)-dependent-NADPH oxidase 1 (NOX1)-reactive oxygen species (ROS) cascade, leading to anti-inflammatory activity. Finally, RME suppressed the production of the BLT2 ligands LTB4 and 12(S)-HETE by inhibiting the p38 kinase-cytosolic phospholipase A₂-5-/12-lipoxygenase cascade in LPS-stimulated Raw264.7 cells. Overall, our results suggest that RME inhibits the 'BLT2 ligand-BLT2'-linked autocrine inflammatory axis, and that this BLT2-linked cascade is one of the targets of the anti-inflammatory action of R. mori.

• 한국자원식물학회지
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• 제33권6호
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• pp.638-644
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• 2020

Lilium lancifolium (LL) is widely cultivated in East Asia and used to attenuate airway diseases. Our current study aimed to investigate the therapeutic effect of LL on pain level and inflammatory response in a rat model of monosodium iodoacetate (MIA)-induced osteoarthritis (OA). We first examined the effect of LL on inflammatory cytokines and inflammatory mediators in IL-1β-treated HTB-94 cells. The LL extract was found to significantly inhibit the levels of Interleukin-6 (IL-6), Interleukin-8 (IL-8), and prostaglandin-E2 (PGE-2) in Interleukin-1 β (IL-1β)-stimulated HTB-94 cells in a dose-dependent manner. Moreover, chronic oral administration of LL effectively restored the weight-bearing distribution in the rat model of MIA-induced OA. In addition, administration of LL inhibited inflammatory cytokines and inflammatory mediators, such as C-reactive protein (CRP), IL-6, leukotriene B4 (LTB-4), PGE-2, and matrix metalloproteinase-9 (MMP-9). Our findings collectively suggested LL as one of the potential therapeutic agents for OA, owing to its properties of reducing pain and inflammatory responses.

• Steel and Composite Structures
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• 제39권5호
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• pp.599-614
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• 2021

This study deals with the Lateral-Torsional Buckling (LTB) of a mono-symmetric tapered I-beam, in which the cross-section is varying longitudinally. To obtain the buckling moment, two concentrated bending moments should be applied at the two ends of the structure. This structure is made of Functionally Graded Material (FGM). The Young's and shear modules change linearly along the longitudinal direction of the beam. It is considered that this tapered beam is laterally restrained continuously, by using torsional springs. Furthermore, two rotational bending springs are employed at the two structural ends. To achieve the buckling moment, Ritz solution method is utilized. The response of critical buckling moment of the beam is obtained by minimizing the total potential energy relation. The lateral and torsional displacement fields of the beam are interpolated by harmonic functions. These functions satisfy the boundary conditions. Two different support conditions are considered in this study. The obtained formulation is validated by solving benchmark problems. Moreover, some numerical studies are implemented to show the accuracy, efficiency and high performance of the proposed formulation.

• 한국강구조학회 논문집
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• 제20권2호
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• pp.237-246
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• 2008

연속경간을 가지는 I형강 교량은 내부 지점근처에서 상대적으로 큰 부모멘트가 발생하게 되는데, 이에 경제적인 단면 활용을 위하여 내부 지점부위의 상부 및 하부플랜지에 플레이트를 보강한 변단면을 사용하고 있다. 본 연구에서는 기존 탄성 횡-비틀림 좌굴식에 관한 연구를 토대로 하여 비탄성 구간에 있는 계단식 I형보의 횡-비틀림 좌굴강도를 범용구조해석프로그램 ABAQUS(2006)를 이용하여 산정하고, 간편한 설계식을 제안하고 있다. 유한요소해석에는 4절점 쉘요소인 S4R이 사용되었고, 국내외에서 많이 사용되는 I형강 단면(${W36{\times}160}$)을 대상으로 하였다. 양단 및 한쪽 끝단에 계단식 단면을 가지는 보에 대해서 고려하였으며, 플랜지 길이방향 비, 너비방향 비, 두께의 비로 계단식 I형보를 나타내었다. 해석에 사용된 매개변수는 각각 27가지 및 36가지 조합이고, 하중조건으로 보의 순수굽힘이 발생하는 균일모멘트를 적용시켰으며, 비탄성 구간범위 내에 있는 비지지 길이에 대하여 구조해석을 수행하였다. 비탄성 횡-비틀림 거동을 보기 위하여 잔류응력 및 초기결함을 고려한 비선형해석을 실시하였는데, Pi(1995)등이 고려한 잔류응력의 형상과 국내 I형강 표준 치수 허용치에 근거하여 부재 길이의 0.1%를 초기제작오차로 고려하였다. 본 연구 결과는 다양한 형식의 I형보가 사용되는 빌딩 및 교량의 경제적이고 합리적인 설계의 근간을 제공해 줄 것이며, 향후 다양한 하중 조건을 가지는 양단 또는 일단 계단식 단면 변화보의 비탄성 횡-비틀림 좌굴강도를 계산할 수 있는 설계식 개발에 적극 활용 될 수 있을 것이다.