1 |
Ruitenberg, K. M., J. R. Gilkerson, J. E. Wellington, D. N. Love, and J. M. Whalley. 2001. Equine herpesvirus 1 glycoprotein D expressed in Pichia pastoris is hyperglycosylated and elicits a protective immune response in the mouse model of EHV-1 disease. Virus Res. 79: 125-135.
DOI
|
2 |
Stoscheck, C. M. 1990. Quantitation of protein. Methods Enz. 182: 50-68.
DOI
|
3 |
Green, E. A., C. Botting, H. M. Webb, T. R. Hirst, and R. E. Randall. 1996. Construction, purification and immunogenicity of antigen-antibody-LTB complexes. Vaccine 14: 949-458.
DOI
ScienceOn
|
4 |
Isaka, M., T. Komiya, M. Takahashi, Y. Yasuda, T. Taniguchi, Y. Zhao, et al. 2004. Recombinant cholera toxin B subunit (rCTB) as a mucosal adjuvant enhances induction of diphtheria and tetanus antitoxin antibodies in mice by intranasal administration with diphtheria-pertussis-tetanus (DPT) combination vaccine. Vaccine 22: 3061-3068.
DOI
ScienceOn
|
5 |
Verweij, W. R., L. de Haan, M. Holtrop, E. Agsteribbe, R. Brands, G. J. van Scharrenburg, and J. Wilschut. 1998. Mucosal immunoadjuvant activity of recombinant Escherichia coli heatlabile enterotoxin and its B subunit: Induction of systemic IgG and secretory IgA responses in mice by intranasal immunization with influenza virus surface antigen. Vaccine 16: 2069-2076.
DOI
ScienceOn
|
6 |
Pizza, M., M. M. Giuliani, M. R. Fontana, E. Monaci, G. Douce, G. Dougan, K. H. Mills, R. Rappuoli, and G. Del Giudice. 2001. Mucosal vaccines: Non toxic derivatives of LT and CT as mucosal adjuvants. Vaccine 19: 2534-2541.
DOI
ScienceOn
|
7 |
Richards, C. M., A. T. Aman, T. R. Hirst, T. J. Hill, and N. A. Williams. 2001. Protective mucosal immunity to ocular herpes simplex virus type 1 infection in mice by using Escherichia coli heat-labile enterotoxin B subunit as an adjuvant. J. Virol. 75: 1664-1671.
DOI
ScienceOn
|
8 |
Tschopp, J. F., G. Sverlow, R. Kosson, W. Craig, and L. Grinna. 1987. High-level secretion of glycosylated invertase in the methylotrophic yeast, Pichia pastoris. BioTechnology 5: 1305-1308.
DOI
|
9 |
Ma, X., W. Zheng, T. Wang, D. Wei, and Y. Ma. 2006. Optimization and high-level expression of a functional GSTtagged rHLT-B in Escherichia coli and GM1 binding ability of purified rHLT-B. J. Microbiol. 44: 293-300.
|
10 |
Holmgren, J., M. Lindblad, P. Fredman, L. Svennerholm, and H. Myrvold. 1985. Comparison of receptors for cholera and Escherichia coli enterotoxins in human intestine. Gastroenterology 89: 27-35.
|
11 |
Cregg, J. M., T. S. Vedvick, and W. C. Raschke. 1993. Recent advances in the expression of foreign genes in Pichia pastoris. BioTechnology 11: 905-910.
DOI
ScienceOn
|
12 |
Rezaee, M. A., A. Rezaee, S. M. Moazzeni, A. H. Salmanian, Y. Yasuda, K. Tochikubo, S. N. Pirayeh, and M. Arzanlou. 2005. Expression of Escherichia coli heat-labile enterotoxin B subunit (LTB) in Saccharomyces cerevisiae. J. Microbiol. 43: 354-360.
|
13 |
Scorer, C. A., J. J. Clare, W. R. McCombie, M. A. Romanos, and K. Sreekrishna. 1994. Rapid selection using G418 of high copy number transformants of Pichia pastoris for high-level foreign gene expression. BioTechnology 12: 181-184.
DOI
ScienceOn
|
14 |
Feil, I. K., R. Reddy, L. de Haan, E. A. Merritt, F. van den Akker, D. R. Storm, and W. G. Hol. 1996. Protein engineering studies of A-chain loop 47-56 of Escherichia coli heat-labile enterotoxin point to a prominent role of this loop for cytotoxicity. Mol. Microbiol. 20: 823-832.
DOI
ScienceOn
|
15 |
Kozuka, S., Y. Yasuda, M. Isaka, N. Masaki, T. Taniguchi, K. Matano, et al. 2000. Efficient extracellular production of recombinant Escherichia coli heat-labile enterotoxin B subunit by using the expression/secretion system of Bacillus brevis and its mucosal immunoadjuvanticity. Vaccine 18: 1730-1737.
DOI
ScienceOn
|
16 |
Douce, G., M. Fontana, M. Pizza, R. Rappuoli, and G. Dougan. 1997. Intranasal immunogenicity and adjuvanticity of sitedirected mutant derivatives of cholera toxin. Infect. Immun. 65: 2821-2828.
|
17 |
Nashar, T. O., H. M. Webb, S. Eaglestone, N. A. Williams, and T. R. Hirst. 1996. Potent immunogenicity of the B subunits of Escherichia coli heat-labile enterotoxin: Receptor binding is essential and induces differential modulation of lymphocyte subsets. Proc. Natl. Acad Sci. U.S.A. 93: 226-230.
DOI
ScienceOn
|
18 |
Fingerut, E., B. Gutter, R. Meir, D. Eliahoo, and J. Pitcovski. 2005. Vaccine and adjuvant activity of recombinant subunit B of E. coli enterotoxin produced in yeast. Vaccine 23: 4685-4996.
DOI
ScienceOn
|
19 |
Sagt, C. M., B. Kleizen, R. Verwaal, M. D. de Jong, W. H. Muller, A. Smits, et al. 2000. Introduction of an N-glycosylation site increases secretion of heterologous proteins in yeasts. Appl. Environ. Microbiol. 66: 4940-4944.
DOI
ScienceOn
|
20 |
Millar, D. G., T. R. Hirst, and D. P. Snider. 2001. Escherichia coli heat-labile enterotoxin B subunit is a more potent mucosal adjuvant than its closely related homologue, the B subunit of cholera toxin. Infect Immun. 69: 3476-3482.
DOI
ScienceOn
|
21 |
Sreekrishna, K., L. Nelles, R. Potenz, J. Cruze, P. Mazzaferro, W. Fish, et al. 1989. High-level expression, purification, and characterization of recombinant human tumor necrosis factor synthesized in the methylotrophic yeast Pichia pastoris. Biochemistry 28: 4117-4125.
DOI
ScienceOn
|
22 |
Isaka, M., Y. Yasuda, S. Kozuka, T. Taniguchi, Y. Miura, K. Matano, N. Goto, and K. Tochikubo. 1999. Intranasal or subcutaneous co-administration of recombinant cholera toxin B subunit stimulates only a slight or no level of the specific IgE response in mice to tetanus toxoid. Vaccine 17: 944-948.
DOI
ScienceOn
|
23 |
Takahashi, I., H. Kiyono, R. J. Jackson, K. Fujihashi, H. F. Staats, S. Hamada, J. D. Clements, K. L. Bost, and J. R. McGhee. 1996. Epitope maps of the Escherichia coli heat-labile toxin B subunit for development of a synthetic oral vaccine. Infect. Immun. 64: 1290-1298.
|
24 |
Amin, T. and T. R. Hirst. 1994. Purification of the B-subunit oligomer of Escherichia coli heat-labile enterotoxin by heterologous expression and secretion in a marine vibrio. Protein Expr. Purif. 5: 198-204.
DOI
ScienceOn
|
25 |
de Haan, L., W. R. Verweij, I. K. Feil, M. Holtrop, W. G. Hol, E. Agsteribbe, and J. Wilschut. 1998. Role of GM1 binding in the mucosal immunogenicity and adjuvant activity of the Escherichia coli heat-labile enterotoxin and its B subunit. Immunology 94: 424-430.
DOI
ScienceOn
|
26 |
Sixma, T. K., S. E. Pronk, K. H. Kalk, E. S. Wartna, B. A. van Zanten, B. Witholt, and W. G. Hol. 1991. Crystal structure of a cholera toxin-related heat-labile enterotoxin from E. coli. Nature 351: 371-377.
DOI
ScienceOn
|
27 |
Cregg, J. M., J. F. Tschopp, C. Stillman, R. Siegel, M. Akong, W. S. Craig, et al. 1987. High-level expression and efficient assembly of hepatitis B surface antigen in the methylotrophic yeast, Pichia pastoris. BioTechnology 5: 479-485.
DOI
|
28 |
Douce, G., C. Turcotte, I. Cropley, M. Roberts, M. Pizza, M. Domenghini, R. Rappuoli, and G. Dougan. 1995. Mutants of Escherichia coli heat-labile toxin lacking ADP-ribosyltransferase activity act as nontoxic, mucosal adjuvants. Proc. Natl. Acad. Sci. U.S.A. 92: 1644-1648.
DOI
ScienceOn
|