• Journal of Ginseng Research
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• v.21 no.1
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• pp.39-42
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• 1997

Dynamic parameters of biochemistry including the contents of soluble carbohydrate (SC) , crude fat (CF) , fatty arid (FA) and soluble protein (SP) in American send (Panax qufnquelolium L.) seed are reported in the present paper. When embryo ratio (ER) increased from 7.31 to 20.48%, the SC content decreased from 4.08 to 1.16%. After that, SC content changed little. The CF content decreased successively from 34.50 to 14.40% from the beginning to the end during the embryo afterripening. The changes of FA content did not correlate with that of ER and the changing range of SP content was not large during the seed stratification. All of these may provide some information for understanding the dormancy mechanisms of American seng seed.

• Korean Journal of Medicinal Crop Science
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• v.18 no.3
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• pp.180-185
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• 2010

We investigated genetic diversity among and within the populations of cultivated ginseng (Panax ginseng C. A. Meyer ) using SRAP profiles. A total of 24 ginseng plants were sampled from the three populations (two from China, one from Korea). Since all these populations are previously shown closely related to each other assister groups, we used Panax quinquefolium L. and wild ginseng as a reference species, which is not "within the sister group". All individuals from the three populations were screened with a total of 36 primer pairs with 26 primers generated from 328 SRAP bands of DNA gels. The mean gene diversity ($H_E$) was estimated to be 0.057 within populations (range 0.032-0.067), and 0.086 at the species level. The genetic differentiation (Gst=0.31) indicates that genetic variation apportioned 30% among populations and 70% within populations. Generally, the result of this study indicates that ginseng contains high molecular variation in its populations.

• Journal of Ginseng Research
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• v.48 no.4
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• pp.354-365
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• 2024

Panax species include Panax ginseng Meyer, Panax quinquefolium L., Panax notoginseng, Panax japonicum, Panax trifolium, and Panax pseudoginseng, which contain bioactive components (BCs) such as ginsenosides and polysaccharides. Recently, growing evidence has revealed the pharmacological effects of Panax species and their BCs on allergic airway diseases (AADs), including allergic asthma (AA) and allergic rhinitis (AR). AADs are characterized by damaged epithelium, sustained acquired immune responses with enforced Th2 responses, allergenspecific IgE production, and enhanced production of histamine and leukotrienes by activated mast cells and basophils. In this review, we summarize how Panax species and their BCs modulate acquired immune responses involving interactions between dendritic cells and T cells, reduce the pro-inflammatory responses of epithelial cells, and reduce allergenic responses from basophils and mast cells in vitro. In addition, we highlight the current understanding of the alleviative effects of Panax species and their BCs against AA and AR in vivo. Moreover, we discuss the unmet needs of research and considerations for the treatment of patients to provide basic scientific knowledge for the treatment of AADs using Panax species and their BCs.

• Korean Journal of Medicinal Crop Science
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• v.14 no.3
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• pp.183-187
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• 2006

This study carried out to analize quality in the evaluation based on the grades such as raw ginseng and red ginseng between Korean (Panax ginseng C. A. Meyer) and American ginseng (Panax quinquefolium L). American ginseng was small in root length, root diameter and root weight and emergence many secondary root from rhizome and main root. Therefore body form was bad compared with Korean ginseng. As for yield of dry, Korean ginseng was 30.4 % and American ginseng was 33.8% but as for yield, Korean ginseng was 80.4% and American ginseng was 72.2%. There were as many Korean ginseng in raw ginseng first grade by about twice compared with American ginseng. However there were many American ginseng in second grade. On the other hand, Korean ginseng producted high Chunsam compared with American ginseng. The cause of decreasing quality was inside cavity and inside white in Korean and American ginseng. Especially, the inside white occurrence about 3 times compared with inside cavity in American ginseng. In the case of distribution of weight size, Korean ginseng was much above 20Ji but American ginseng was much under 20Ji.

• Journal of Ginseng Research
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• v.14 no.2
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• pp.297-309
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• 1990

Ginseng is renowned for both its medicinal and herbal uses and successful cultivation of Panax ginseng in Asia and Panax Vtiinvtiefolilim in North America has until recently taken place in the native geographical ranges of the plants. As a consequence of the potential high capital return and anticipated increases in consumer consumption, commercial cultivation of American ginseng now occurs well outside the native range of the plant in North America. In fact, the region of greatest expansion of cultivation is in the semi-arid interior region of British Columbia, Canada. Linked with this expansion is the potential domination of the ginseng industry by agricultural corporations. In the interior of British Columbia, the native decidous forest environment of eastern North America is simulated with elevated polypropylene shade and a sllrface covering of straw mulch. The architecture of these environments is designed to permit maximillm machinery useage and to minimize labour requirements. Further, with only a four-year growth cycle, plant densities in the gardens are high. In this hot, semiarid environment, producers believe they have a competitive advantage over other regions in North America because of the low precipitation rates. This helps to minimize atmospheric humidity such that the conditions for fungal disease development are reduced. If soil moisture levels become limited, supplemental water can be provided by irrigation. The nature of the radiation and energy balance regimes of the shade and much environment promotes high soil moistilre levels. Also, the modified environment reduces soil heating. This can result in an aerial environment for the plant that is stressful and a rooting zone environment that is sub-optimal. The challenge of further refining the man modified environment for enhanced plant growth and health still remains.

• Proceedings of the Korean Society of Applied Pharmacology
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• 2003.11a
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• pp.108-108
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• 2003

The current study was performed to observe the effects of Panax ginseng (PG) and P. quinquefolia (PQ) on hemodynamics such as blood flow rate (BF), blood flow velocity (BV), heart rate (HR), systolic blood pressure (SBP) and diastolic blood pressure (DBP, and body temperature (BT) in healthy young men. After testing equality of variance, Student's t-test using PROC TTEST was examined to. prove statistical differences between control and ginseng conditions at each time point. It was found that the BF data were fluctuated by personal deviation. In order to minimize the deviation, the results obtained for 6 hrs were reconstituted after dividing them into two periods of the first half from 1 to 3 hrs and of the latter half from 3.5 to 6 hrs. And then the reconstitution data and dose-response curves were obtained. Blood flow such as BF and BV shows significant increases both two periods in the dose of PG 2.25 and PG 9.0, whereas significant decrease in the dose of PG 4.5. However, in the PQ groups, the middle dose PQ 4.5 shows the highest significant increase among the three doses. Except for PG 2.25 in HR, other doses show significant decreases both in the first half and latter half. SBP of PQ 9.0 shows only a significant decrease in the first half; on the other hand, in the latter half, PG 4.5, PG 9.0 and PQ 9.0 significantly increase SBP. In addition, DBP of PG 2.25 and PG 4.5 show significant increase in the both periods. In the BT, PQ groups show gradual decrease from PQ 2.25 to PQ 9.0; however, PG groups show differently. PG 4.5 shows significant decrease, but PG 9.0 shows a increase without statistical meanings. In summary, PG is more effective in respect to keeping homeostasis of hemodynamics.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.18
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• pp.7919-7923
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• 2014

20(S)-Protopanaxadiol (PPD), a ginsenoside isolated from Pananx quinquefolium L., has been shown to inhibit growth and proliferation in several cancer cell lines. The aim of this study was to evaluate its anticancer activity in human breast cancer cells. MCF-7 cells were incubated with different concentrations of 20(S)-PPD and cytotoxicity was evaluated by MTT assay. Occurrence of apoptosis was detected by DAPI and Annexin V-FITC/PI double staining. Mitochondrial membrane potential was measured with Rhodamine 123. The Bcl-2 and Bax expression were determined by Western blot analysis. Caspase activity was measured by colorimetric assay. 20(S)-PPD dose-dependently inhibited cell proliferation in MCF-7 cells, with an $IC_{50}$ value of $33.3{\mu}M$ at 24h. MCF-7 cells treated with 20(S)-PPD presented typical apoptosis, as observed by morphological analysis in cell stained with DAPI. The percentages of annexin V-FITC positive cells were 8.92%, 17.8%, 24.5% and 30.5% in MCF-7 cells treated with 0, 15, 30 and $60{\mu}M$ of 20(S)-PPD, respectively. Moreover, 20(S)-PPD could induce mitochondrial membrane potential loss, up-regulate Bax expression and down-regulate Bcl-2 expression. These events paralleled activation of caspase-9, -3 and PARP cleavage. Apoptosis induced by 20(S)-PPD was blocked by z-VAD-fmk, a pan-caspase inhibitor, suggesting induction of caspase-mediated apoptotic cell death. In conclusion, the 20(S)-PPD investigated is able to inhibit cell proliferation and to induce cancer cell death by a caspase-mediated apoptosis pathway.

• Proceedings of the Ginseng society Conference
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• 1984.09a
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• pp.49-55
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• 1984

Dormant one-year-old ginseng roots were subjected to a range of stratification temperatures and time to define effective limits to these parameters and to quantify their effect on terminating dormancy. Effective storage temperatures tested ranged from $0^{\circ}C\;to\;9^{\circ}C.$ A low percentage of roots produced tops with as few as 30 days in stratification; however, 75 to 90 days were required for 100 percent emergence. Days to emergence, after planting, decreased with increased days in storage thru the maximum storage time of 120 days. The number of days of dormancy was relatively constant, near 126.5 days, over the range of effective temperatures and acceptable storage times. The minimum period of dormancy was associated with 75 days in storage at $3^{\circ}C.$ Root growth rate, after emergence, was greatest following 105 days of stratification. The frequency distribution of emergence with days in stratification suggests the potential of selecting for strains of ginseng with low chilling needs for satisfying dormancy requirements.

• Korean Journal of Plant Resources
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• v.17 no.2
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• pp.116-121
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• 2004

Panax ginseng discarded and lower than 4th grade is caused by red skin disease showing red color skin in ginseng. This kind of red skin ginseng is found a lot in Panax ginseng rather than Panax quinquefolium, and it is considered that red skin disease might be caused by gene. Therefore, this study was carried out to detect genes resistant to red skin disease using RT-PCR. RNA was extracted from three years old ginseng root of both red skin and normal portion in the same root. After RNA extraction, PCR amplification was performed from cDNA using many random primers. As a result, specific band for red skin was found. It is considered that the gene forming band has possibility to be related with red skin disease, and this gene should be decided if it's related with red skin disease. If that gene is related with red skin disease, it will be used for transformation to foster for resistance to red skin disease as well as for selection marker. Bowever, if it's not related with red skin disease, more primers should be used to find gene related with red skin disease.

• Preventive Nutrition and Food Science
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• v.15 no.4
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• pp.275-281
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• 2010

The chemical characteristics of seed oils of Asian ginseng (Panax ginseng C.A. Meyer) at different ages grown in Korea (3, 4 and 5-year old) and China (5-year old), and American ginseng (Panax quinquefoliu L., 5-year old) grown in China were compared. Total fatty acid composition showed a significantly higher oleic acid content in American (87.50%) than in Korean (68.02~69.14%) and Chinese ginseng seed oils (61.19%). At the sn-2 position, the highest oleic acid (81.09%) and lowest linoleic acid (15.77%) were found in American ginseng seed oil. The main triacylglycerol species in ginseng seed oils were triolein (OOO) and 1,2-dioleoyl-3-linoleoyl-glycerol (LOO)/1,3-dioleoyl-2-linoleoyl-glycerol (OLO). In addition, the seed oils possessed an ideal oxidative stability showing 16.55~23.12 hr of induction time by Rancimat test. The results revealed that ginseng seed oil could be developed as a new healthy edible oil, and that the oil's chemical characteristics were strongly associated with the ginseng species and habitats.