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Genetic Diversity and Genetic Structures in Ginseng Landraces (Cultivars) by SRAP Analysis  

Xu, Young Hua (College of Chinese Medicinal Materials, Jilin Agricultural University)
Jin, Hui (Jilin Institute of Ginseng Research)
Kim, Young-Chang (Department of Herbal Crop Research, NIHHS, RDA)
Bang, Kyong-Hwan (Department of Herbal Crop Research, NIHHS, RDA)
Cha, Seon-Woo (Department of Herbal Crop Research, NIHHS, RDA)
Zhang, Lian Xue (College of Chinese Medicinal Materials, Jilin Agricultural University)
Publication Information
Korean Journal of Medicinal Crop Science / v.18, no.3, 2010 , pp. 180-185 More about this Journal
Abstract
We investigated genetic diversity among and within the populations of cultivated ginseng (Panax ginseng C. A. Meyer ) using SRAP profiles. A total of 24 ginseng plants were sampled from the three populations (two from China, one from Korea). Since all these populations are previously shown closely related to each other assister groups, we used Panax quinquefolium L. and wild ginseng as a reference species, which is not "within the sister group". All individuals from the three populations were screened with a total of 36 primer pairs with 26 primers generated from 328 SRAP bands of DNA gels. The mean gene diversity ($H_E$) was estimated to be 0.057 within populations (range 0.032-0.067), and 0.086 at the species level. The genetic differentiation (Gst=0.31) indicates that genetic variation apportioned 30% among populations and 70% within populations. Generally, the result of this study indicates that ginseng contains high molecular variation in its populations.
Keywords
Ginseng; SRAP; Genetic Variance;
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1 Slatkin M. (1985). Rare alleles as indicators of gene flow. Evolution. 39:53-65.   DOI   ScienceOn
2 Rohlf J. (1998). NTSYS-pc. Numerical Taxonomy and Multivariate Analysis System, ver. 2.00. Exeter Software, Setauket, New York.
3 Schaal BA, Hayworth DA, Olsen KM, Rauscher JT and Smith WA. (1998). Phylogeographic studies in plants: problems and prospects. Molecular Ecology. 7:465-474.   DOI   ScienceOn
4 Seo SD, Yuk JA, Cha SK, Kim HH, Seoung BJ, Kim SI, Choi JE. (2003). Analysis of diversity of Panax ginseng collected in Korea by RAPD technique. Korean Journal of Medicinal Crop Science. 11:377-384.   과학기술학회마을
5 Li G and Quiros CF. (2001). Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theoretical and Applied Genetics. 103: 455-461.   DOI   ScienceOn
6 Ma XJ, Wang XQ, Sun SS, Xiao PG and Hong DY. (1999). A study on RAPD fingerprinting of wild mountain ginseng (Pana.x ginseng). Acta Pharmaceutica Sinica. 34:312-316.
7 Ngan F, Shaw P, But P and Wang T. (1999). Molecular authentication of Panax species. Phytochemistry. 50:787-791.   DOI   ScienceOn
8 Fisher M, Husi R, Prati D, Peintinger M, Kleunen M and Schmid B. (2000). RAPD variation among and within small and large populaitons of the rare clonal plant Ranunculus repens (Ranunculaceae). American Journal of Botany. 87:1128- 1137.   DOI   ScienceOn
9 Francis CY and Yang RC. (2000). Popgene version 1.32. (http: www.ualberta.ca/_fyeh/index.htm)
10 In DS, Kim YC, Bang KH, Chung JW, Kim OT, Hyun DY, Cha SW, Kim TS, Seong NS. (2005). Genetic relationships of Panax species by RAPD and ISSR analyses. Korean Journal of Medicinal Crop Science. 13: 249-253.   과학기술학회마을
11 Kim CK and Cho HK. (2003). Genetic diversity and relationship in Korean ginseng (Panax ginseng) based on RAPD analysis. Korean Journal of Genetics. 25:181-188.
12 Excoffier L. (1992). WinAMOVA; Analysis of Molecular Variance, ver. 1.55. Genetic and Biometry Laboratory, University of Geneva.
13 Fisher M and Matthies D. (1998). RAPD variation in relation to population size and plant fitness in the rare Gentianella germanica (Gentianaceae). American Journal of Botany. 85: 811-819.   DOI   ScienceOn
14 Cheung KS, Kwan HS, But BPH and Shaw P. (1994). Pharmacognostical identification of American and oriental ginseng roots by genomic fingerprinting using arbitrarily primed polymerase chain reaction (AP-PCR). Journal of Ethnopharmacology. 42:67-69.   DOI   ScienceOn
15 Doyle JJ and Doyle JL. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemistry Bulletin. 19: 11-15.
16 Bassam BJ, Caetano AG and Gresshoff PM. (1991). Fast and sensitive silver staining DNA in polyacrylamide gels. Analytical Biochemistry. 196:80-83.   DOI   ScienceOn
17 Caccone A. (1985). Gene flow in cave arthropods: A qualitative and quantitative approach. Evolution. 39:1223-1235.   DOI   ScienceOn
18 Carlson AW. (1986). Ginseng: America's botanical drug connection to the Orient. Economic Botany. 40:233-249.   DOI
19 Waples RS. (1987). A multispecies approach to the analysis of gene flow in marine shore fishes. Evolution. 41:385-400.   DOI   ScienceOn
20 Zhuravlev YN, Reunova GD, Artyukova EV, Kozyrenko MM and Muzarok TI. (1998). Genetic variation of wild ginseng populations (RAPD analysis). Molecular Biology. 32:910-914.
21 Sun ZD, Wang ZN, Tu JX, Zhang JF, Yu FQ, McVetty PBE and Li GY. (2007). An ultradense genetic recombination map for Brassica napus, consisting of 13551 SRAP markers. Theoretical and Applied Genetics. 114:1305-1317.   DOI   ScienceOn
22 Slatkin M. (1987). Gene flow and the geographic structure of natural populations. Science. 236:787-792   DOI
23 Slatkin M. (1981). Estimating levels of gene flow in natural populations. Genetics. 99:323-335.