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PCR-T- RFLP Analyses of Bacterial Communities in Activatced Sludges in the Aeration Tanks of Domestic and Industrial Wastewater Treatment Plants

  • RHO SANG CHUL;AN NAN HEE;AHN DAE HEE;LEE KYU HO;LEE DONG HUN;JAHNG DEOK JIN
    • Journal of Microbiology and Biotechnology
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    • v.15 no.2
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    • pp.287-295
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    • 2005
  • In order to compare bacteria] community structure and diversity in activated sludges, terminal restriction fragment length polymorphism (T-RFLP) of PCR-amplified 16s rDNAs was analyzed for 31 domestic and industrial wastewater treatment plants (WTPs). Regardless of the characteristics of the wastewaters, the bacteria] community structures of activated sludges appeared diverse and complex. In particular, activated sludges in domestic WTPs contained higher bacterial diversity than those in industrial WTPs. It was also found that terminal restriction fragment (T-RF) profiles derived from domestic WTPs were very similar with each other, although activated sludges were collected from different plants at different locations. Interestingly, activated sludges of a WTP where restaurant and toilet sewages of a company were managed showed a bacterial community structure similar to that of domestic WTPs. Activated sludges in leather industria] WTPs also showed a high similarity. However, other wastewaters possessed different bacterial communities, so that overall similarity was as low as about $30\%$. Since activated sludges from WTPs for domestic wastewaters and a company sewage appeared to hold similar bacterial communities, it was necessary to confirm if similar wastewaters induce a similar bacterial community. To answer this question, analysis of T-RFs for activated sludges, taken from another 12 domestic WTPs, was conducted by using a 6­FAM$^{TM}$-Iabeled primer and an automated DNA sequencer for higher sensitivity. Among 12 samples, it was again found that T-RF profiles of activated sludges from Yongin, Sungnam, Suwon, and Tancheon domestic WTPs in Kyonggi-do were very similar with each other. On the other hand, T-RF profiles of activated sludges from Shihwa and Ansan WTPs were quite different from each other. It was thought that this deviation was caused by wastewaters, since Ansan and Shihwa WTPs receive both domestic and industrial wastewaters. From these results, it was tentatively concluded that similar bacterial communities might be developed in activated sludges, if WTPs treat similar wastewaters.

The Effects of Application of Meta-problems on Elementary School Students' Mathematical learning (메타문제의 적용이 초등학생의 수학 학습에 미치는 효과)

  • Baek, Myung-Sook;Shin, Hang-Kyun
    • Journal of Elementary Mathematics Education in Korea
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    • v.11 no.1
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    • pp.43-59
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    • 2007
  • The goal of this thesis was to examine the effects of applying meta-problems to elementary school mathematics class In their achievements, beliefs and attitudes. To achieve this goal the following research questions were asked. a. What effects does the class applied with meta-problem have on students' mathematical achievements? b. What effects does the class applied with meta-problem have on students' mathematical beliefs and attitudes? To answer questions, an experimental study was designed and conducted. The subjects were 6th-grade students at S Elementary School located in Dobong-Gu, Seoul where the researcher teaches. Among them, the class that the researcher teach was chosen as the experimental group. During the experimental study, a teaching-learning with meta-problems was applied to the experimental group and a teaching-learning with general problems was applied to the comparative group. To examine changes in the mathematical achievements of the experimental group and the comparative group, a post-test of mathematical achievements was conducted and the results were t-tested. As well, to find answers to the second research question, a pre-test and a post-test of mathematical beliefs and attitudes were conducted on the experimental group and the results were t-tested. The results of this study were as follows First, the experimental group which was taught applying meta-problems got higher mathematical achievement than the comparative group. Second, the class with meta-problems did not bring significant changes in students' mathematical beliefs and attitudes. Synthesizing the study results above, a teaching-learning with meta-problems is a teaching-learning method that can accommodate problem solving naturally in school mathematics and give a positive effect on students' mathematical achievements.

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A Study on the Empirical Basis of Prejudice towards the Credibility of Persons under Investigation (수사관 편견의 실증적 근거에 관한 연구 - 교통사고 피조사자의 인구사회학적 특성에 따른 수사관의 편견을 중심으로 -)

  • Lee, Ju-Lak;Tark, Jong-Eon
    • Korean Security Journal
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    • no.15
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    • pp.285-301
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    • 2008
  • Police officers have an obligation to their profession to continuously strive for fairness for all the people they serve. However, some police officers are believed to have developed prejudicial attitudes towards some groups of the society and exercised police power unfairly against them. Especially, when the actions of the police are prejudicial during criminal investigation, this can affect outcomes of the investigation. Therefore, the police need to tackle this problem effectively. In order to develop a preventive strategy, the police must have a clear understanding of prejudice. However, there has been little research on this topic in Korea. Thus, this study attempted to fill the gap. The purpose of the current study was to examine if prejudice of police officers are statistically valid. In order to answer this research question, the present study utilized the results of 173 polygraph examinations that had been employed to investigate traffic accidents in a provincial police agency in 2006. From the polygraph examination reports, information relating to the subjects could be identified and this information was analyzed, using a logistic regression. The results of the logistic regression revealed that most of the variables relating to the subjects did not affect the credibility of the subjects' statements. This means the police officers' belief that some groups of people make false statements more often than others during criminal investigation is an unfounded prejudice.

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Media Role in the Transition and Consolidation Period of Democracy: A Comparative Study of Korea and Spain (민주주의의 이행 및 공고화 과정에서 미디어의 역할: 한국과 스페인의 비교)

  • Cho, Hang-Je
    • Korean journal of communication and information
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    • v.18
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    • pp.269-303
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    • 2002
  • This article presents a historical account of media role in the transition and consolidation period of democracy. Despite the assumption that media plays a important role in the construction of democracy, it is less clear how the media can affect the process of political change itself. This article seeks to answer some of these question, based on the Mill's macro-social comparative 'method of difference' of Korea and Spain. It is widely agreed that both states achieve democracy through transaction from above(pacts). Media role, however, differs significantly in accordance with authoritarian legacies and civic representativeness of the pacts. Whereas Korean dailies is deepening given market oligopoly and prior practices after democratization, Spain dailies market entirely changed in both structural and spiritual respects. As a result, Korean dailies substantially lacks in civic representativeness as before, contrary to Spain. Spain television settled a sort of the external pluralism. Korean television is pursuing the BBC type of internal pluralism. In Korea, television is more commercial than Spain. Consequently, Spain media serve the consolidation of democracy more than Korea on the whole.

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Estimating Method of Starting Point Bias in Bidding Game (서베이를 이용한 입찰게임에서 출발점 편의의 추정)

  • 박용치
    • Survey Research
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    • v.4 no.2
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    • pp.63-86
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    • 2003
  • The objective of this study was to investigate the existence of starting point bias in the bidding game contingent valuation elicitation technique when determining willingness to pay (WTP) for improving the qualify of running water in Seoul and its vicinity. Of all existing contingent techniques, the bidding game most closely mimics the normal price taking behavior in local markets. Three different starting points (low, medium and high) were used to determine WTP and the existence of starting point bias in the meanwhile respectively. The respondents were randomly assigned to the three different starting point groups in order to ensure homogeneity, so that any variation seen in WTP could be attributed to the starting point effects. And a pretested interviewer-administered questionnaire used to elicit WTP. Non-parametric test and the logit model were used to analyze the data for evidence of starting point bias. In this instance, the high starting point group had a high WTP, and low starting point group had a low WTP. This means there exist starting point bias in estimating WTP by bidding game in this instance. This finding might signal that people may actually be making up their minds on the maximum amount they are willing to pay for running water service as a bidding iterating is going on and is influenced by the starting point used in the bidding game. The problem of slaking point bias can be avoided if the respondent is asked directly for the maximum WTP without payment cards or a bidding game. But such a question is perceived as being very difficult to answer and this leads to problems of non-response and being unrealistic.

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Poverty in Korea, Why It Remains High?: Analysis of the Trend in Poverty since the 1990s (한국의 빈곤, 왜 감소하지 않는가? - 1990년대 이후 빈곤 추이의 분석 -)

  • Ku, In-Hoe
    • Korean Journal of Social Welfare
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    • v.56 no.4
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    • pp.57-78
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    • 2004
  • The economic crisis in $1997{\sim}1998$ caused massive unemployment and unprecedentedly increased the number of the poor in Korea. As many unemployed families fell into poverty, the poverty rate skyrocketed to higher than 10 percent. Not later than 2000, unemployment late got back to normal and real average income among urban households approached to the income level prior to the economic crisis. Although the economic crisis has been passed through, poverty was not decreased to the low level prior to the crisis by 2000. Why does it remain high? This study attempts to provide an answer to this question by analysing the poverty trend over the 1990s. Data come from the National Survey of Household Income and Expenditures 1991, 1996, and 20001. Results show that poverty was rapidly reduced in the first half period of the 1990s. This reduction in poverty is largely explained by steady and rapid economic growth. Modest improvement in income inequality also contributed. In contrast, the poverty rate considerably increased in the latter half of the 1990s. Average income was not fully recovered to its prior level, which reflected the economic crisis and the subsequent economic stagnation. Worsened income inequality led to higher poverty rate too. In addition, demographic changes increased the share of economically vulnerable types of families, such as families headed by single parents and the elderly. The most significant factor in explaining the higher poverty rate was extended income differential among non-elderly adults, while the next was the increased number of the elderly families. Yet, findings a little differ depending on which concepts of poverty to adopt. In the analyses based on the concept of absolute poverty, economic growth the most significantly affected the poverty trends in the 1999s. Changes in income inequality played the most important role in explaining the trend in relative poverty. Adopting the concepts of quasi-absolute poverty, which is preferred in this study, results show that rapid economic growth significantly reduced poverty in the first half of the 1990s and both worsened income inequality and stagnated economic growth increased poverty in the latter 1990s.

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A Causal Model on the Relationship between Resources of Natural Parks and User's Satisfaction (자연공원의 자원과 이용 만족도간의 관계에 관한 인과모형 -국립공원과 도립공원을 중심으로-)

  • 장병문;배민기
    • Journal of the Korean Institute of Landscape Architecture
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    • v.30 no.3
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    • pp.12-24
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    • 2002
  • The purpose of this paper is to decompose the effect of resources of natural parks(NP) on user's satisfaction to answer the research question: What are the causal effects of resources of natural parks on user\ulcorner After reviewing the literature, classification of resources of NP, various approaches and analysis methods employed, we constructed the conceptual framework and have formulated the hypothesis of this research. We had obtained data through a questionnaire, which surveyed 414 visitors at 6 of the 73 NP in Korea in 2001, based on a stratified sampling method. We have analyzed the data using descriptive statistical methods, Pearson's correlation analysis, and a path analysis method. We found that 1) While the indirect effect of topographical feature and valley(TFV), socio-cultural resources(SCR), and climate, sound, and scent(CSS) turned out to be 2.75, 1.20, and 2.00 times higher than that of wild animal and plant(WAP), the direct effect of TFV, SCR, and landscape turned out to be 2.95, 2.88, and 2.64 times higher than that of CSS, 2) The magnitude of causal effects of the three exogeneous variables of TFV, WAP, and SCR and two intervening variables of CSS and landscape on User's satisfaction turned out to be 0.403, 0.048, 0.323, 0.188, and 0.243, respectively, 3) Total direct effect of the exogeneous and intervening variables on user's satisfaction is 0.871, while that of indirect effect is 0.334, and 4) Causal effect of tangible resources is 1.80 times higher than that of intangible while total effect of tangible resources are 1.36 times higher than that of intangible. The research results suggest that 1) Criteria for designation and maintenances of NP and results of previous studies on resources turned out to be unreliable and distorted, 2) In the criteria of planning and maintenance of NP, intangible resources must be included, 3) Remedial directions to increase user's satisfaction should be focused on maintenance of TFV and landscape in NP, and 4) The approach and path analysis adopted by this research is valid and highly useful for other resource based recreation area. It is recommended that more empirical study on seasonal variation of resources in NP based user's preference be performed in the future.

The Research of Knowledge and Attitude about Water Fluoridation Program for Residents of Haeundae-Gu, Busan (부산광역시 해운대구민의 수돗물불소농도조정사업에 대한 의식 조사연구)

  • Jeon, Eun-Suk;Lee, Hye-Jin;Bae, Soo-Myung
    • Journal of dental hygiene science
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    • v.6 no.1
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    • pp.47-53
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    • 2006
  • This study is performed both for notification of knowledge level about water fluoridation program for residents of Haeundae, Busan and for finding out the purpose of solving methods of problems which could appeared in water fluoridation program, it has resulted by the survey of 460 Haeundae citizens from August 5, 2005 thru 20th of same month. 1. The drinking water for Haeundae Residents is occupied of 42.8% of the purified water and 31.3% of public tap water, among these, the cooking water is consist of 48.3% of the tap water and 35.4% of the purified water. 2. 35.7% of participants has already known about the water fluoridation program, but 42.0% hasn't, 22.4% were no answer. 3. For the question why water fluoridation program has done 42.0% answered for caries prevention, 32.3% for disinfection of tap water and 15.0% for tap water purification. 4. For water fluoridation program, 78.0% approved and 22.0% disapproved, among reasons 51.0% of approvals were caries prevention and 38.6% of disapproval were that fluorine can be harmful for the human body. 5. There is a fact that fluorine is contained in food, beverage, water, and air, only 21.3% has notified that fact, on the other hand 78.7% hasn't, among teens, 93.5% doesn't know the fluorine containing facts. 6. In the safety notification rate of water fluoridation program, 39.3% notifies it safe, 55.7% showed unknown response, and the speech 28.3% may be the best way to notify it.

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Dental students' clinical confidence in denture treatment after clinical experiences (의치진료 경험 후 치과대학 학생들의 임상진료능력에 대한 자기인식도)

  • Lee, Bora;Shim, June-Sung;Lee, Jae Hoon;Kim, Jong-Eun;Park, Ji-Man;Kim, Jee-Hwan
    • The Journal of Korean Academy of Prosthodontics
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    • v.58 no.3
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    • pp.185-192
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    • 2020
  • Purpose: The purpose of this study is to investigate dental students' self-perception of clinical care ability after a denture treatment experience. Materials and methods: Of the 58 fourth-year students at the dental school in 2019, 50 students completed the questionnaire concerning their denture treatment session, including treatment planning and pre-prosthetic treatment (4 questions), clinical and laboratory procedures (20 questions), and students' opinions on clinical denture education (4 questions). Each question was answered on a five-point scale, of which points four and five, "agree" and "strongly agree" respectively, were classified as positive responses while points one and two, "disagree" and "strongly disagree" respectively, were classified as negative. Results: All the items on clinical and laboratory procedures received a positive response rate of over 60%, and the overall self-perception of the students appeared to be positive. However, the questions on the ability to perform denture treatment independently after graduation received only 48% of positive answer. Those on patient management, explanations of denture precautions, customized tray production, boxing, and work model production were answered with the highest positive response rate whereas those on treatment planning, final impression acquisition, and surveying, were the lowest in each session. Conclusion: The dental students with experience in denture treatment generally have a positive opinion of their clinical care ability, but further education may be necessary to improve their ability to treat their patients independently.

Development of PBL Package - focusing on dental hygienist roles - (치과위생사 역할중심의 문제중심학습 패키지 개발)

  • Jung, Young-Ran;Hwang, Yoon-Sook
    • Journal of Korean society of Dental Hygiene
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    • v.4 no.1
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    • pp.119-132
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    • 2004
  • The purpose of this study was to introduce PBL to dental hygienist education in an effort to raise a question about the inauthentic and inappropriate curriculum. PBL is one of learning methods to enhance the problem-solving ability of learners, and it's attempted to develop a PBL package focusing on dental hygienist roles to lay the foundation for producing competent and expert dental hygienists with a good problem- solving ability. The literature concerned was reviewed from November 2002 through January 2003 to determine whether or not PBL was applicable to dental-hygienist course, and that turned out to be effective for dental hygienist education. And then a PBL package was developed to train students to be knowledgeable and have a knowhow and excellent problem-solving skills. The characteristics of the PBL package could be described as follows: First, that focused on dental hygienist roles to serve the purpose of this study to remedy the current unrealistic and improper curriculum and improve the problem-solving skills of learners. Second, time factor was taken into account. In this four-week course for two credits, there are four classes a week, and it's required to take six or eight weeks to apply the PBL package, which is expected to demotivate students. Therefore, it's planned to conduct more weekly classes to make a proper progress. Third, a wide variety of teaching aids were put to use, and learner would be encouraged to be more interactive and utilize teaching aids properly, and eventually, they could have an opportunity to better express themselves. Fourth, online real-time learner discussion would be attended by this researcher. Learners would have a discussion in real time in the Internet cafe chat room, and different discussion time would be allocated to each team. This researcher would take part in each team's discussion once or more. Fifth, learners would prepare one or more journal(s) about four-hour Internet cafe learning. They have to make it twice a week at least, and it would be a good opportunity for learners to look back on themselves and their teams, and their learning effect would be greater. Specific rules were presented to help them make a successful self-examination. Sixth, there are some spaces in the lower part of objective test sheets to have students describe why they make a particular answer choice. They would be asked to depict the reason of their prior evaluation and lecture assessment especially because their responses would be important for more successful discussion and feedback. Seventh, problem-solving approach was designed to attain learning objectives, stimulate the creative thinking of learners and help them share a more systematic discussion. That would serve as a secondhand guide not to make them digress when they discuss by using information they acquire from a scenario presented in class.

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