• Asian-Australasian Journal of Animal Sciences
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• 제30권2호
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• pp.275-283
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• 2017

Objective: An experiment was conducted to investigate the environment of the deep litter system and provided theoretical basis for production. Methods: The bedding samples were obtained from a pig breeding farm and series measurements associated with gases concentrations and the bacterial diversity as well as the quantity of Escherichia coli, Lactobacilli, Methanogens were performed in this paper. Results: The concentrations of $CO_2$, $CH_4$, and $NH_3$ in the deep litter system increased with the increasing of depth while the $N_2O$ concentrations increased fiercely from the 0 cm to the -10 cm depth but then decreased beneath the -10 cm depth. Meanwhile, the Shannon index, the dominance index as well as the evenness index at the -20 cm layer was significantly different from the other layers (p<0.05). On the other hand, the quantity of Escherichia coli reached the highest value at the surface beddings and there was a significant drop at the -20 cm layer with the increasing depth. The Lactobacilli numbers increased with the depth from 0 cm to -15 cm and then decreased significantly under the -20 cm depth. The expression of Methanogens reached its largest value at the depth of -35 cm. Conclusion: The upper layers (0 cm to -5 cm) of this system were aerobic, the middle layers (-10 cm to -20 cm) were micro-aerobic, while that the bottom layers (below -20 cm depth) were anaerobic. In addition, from a standpoint of increasing the nitrification pathway and inhibiting the denitrification pathway, it should be advised that the deep litter system should be kept aerobic.

• Asian-Australasian Journal of Animal Sciences
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• 제33권4호
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• pp.651-661
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• 2020

Objective: We hypothesized that Cr source can alter adipogenic-related transcriptional regulations and cell signaling. Therefore, the objective of the study was to evaluate the biological effects of chromium acetate (CrAc) on bovine intramuscular (IM) and subcutaneous (SC) adipose cells. Methods: Bovine preadipocytes isolated from two different adipose tissue depots; IM and SC were used to evaluate the effect of CrAc treatment during differentiation on adipogenic gene expression. Adipocytes were incubated with various doses of CrAc: 0 (differentiation media only, control), 0.1, 1, and 10 μM. Cells were harvested and then analyzed by real-time quantitative polymerase chain reaction in order to measure the quantity of adenosine monophosphate-activated protein kinase-α (AMPK-α), CCAAT enhancer binding protein-β (C/EBPβ), G protein-coupled receptor 41 (GPR41), GPR43, peroxisome proliferator-activated receptor-γ (PPARγ), and stearoyl CoA desaturase (SCD) mRNA relative to ribosomal protein subunit 9 (RPS9). The ratio of phosphorylated-AMPK (pAMPK) to AMPK was determined using a western blot technique in order to determine changing concentration. Results: The high dose (10 μM) of CrAc increased C/EBPβ, in both IM (p = 0.02) and SC (p = 0.02). Expression of PPARγ was upregulated by 10 μM of CrAc in IM but not in SC. Expression of SCD was also increased in both IM and SC with 10 μM of CrAc treatment. Addition of CrAc did not alter gene expression of glucose transporter 4, GPR41, or GPR43 in both IM and SC adipocytes. Addition of CrAc, resulted in a decreased pAMPKα to AMPKα ration (p<0.01) in IM. Conclusion: These data may indicate that Cr source may influence lipid filling in IM adipocytes via inhibitory action of AMPK phosphorylation and upregulating expression of adipogenic genes.

• 한국초등수학교육학회지
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• 제23권3호
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• pp.273-288
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• 2019

본 연구의 목적은 식 a:b=c:d의 의미 구조를 분석하고 학습 지도에의 시사점을 확인하는 것이다. 분석 결과, 식 a:b=c:d는 다음과 같은 다중의 의미를 지니고 있다. 한 상황에 내재된 다른 비 구조, 다른 상황에 내재된 공통된 비 구조, 다른 상황에 내재된 제3의 수량의 같음. 식 a:b=c:d의 의미 학습 지도에서 단위를 유연하게 설정하여 한 상황에서 다른 구조 보기와 다른 상황에서 같은 구조 보기, 이중테이프 모델 사용, 양의 속성의 차이에 따른 비율의 의미와 그 중요성이 강조될 필요가 있다. a:b=c:d의 전체 의미 구조에 비추어 보면, 우리나라에서 이루어져 온 비례식 의미 학습 지도는 식 a:b=c:d의 의미의 한정된 부분을 제한된 방식으로 다루고 있다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권16호
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• pp.7205-7210
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• 2015

Background: In this study, influence caused by expression plasmids of connective tissue growth factor (CTGF) and tissue inhibitor of metalloproteinase-1 (TIMP-1) short hairpin RNA (shRNA) on mRNA expression of CTGF,TIMP-1,procol-${\alpha}1$ and PCIII in hepatic tissue with hepatic fibrosis, a precancerous condition, in rats is analyzed. Materials and Methods: To screen and construct shRNA expression plasimid which effectively interferes RNA targets of CTGF and TIMP-1 in rats. 50 cleaning Wistar male rats are allocated randomly at 5 different groups after precancerous fibrosis models and then injection of shRNA expression plasimids. Plasmid psiRNA-GFP-Com (CTGF and TIMP-1 included), psiRNA-GFP-CTGF, psiRNA-GFP-TIMP-1 and psiRNA-DUO-GFPzeo of blank plasmid are injected at group A, B, C and D, respectively, and as model control group that none plasimid is injected at group E. In 2 weeks after last injection, to hepatic tissue at different groups, protein expression of CTGF, TIMP-1, procol-${\alpha}1$ and PC III is tested by immunohistochemical method and,mRNA expression of CTGF,TIMP-1,procol-${\alpha}1$ and PCIII is measured by real-time PCR. One-way ANOVA is used to comparison between-groups. Results: Compared with model group, there is no obvious difference of mRNA expression among CTGF,TIMP-1,procol-${\alpha}1$, PC III and of protein expression among CTGF, TIMP-1, procol-${\alpha}1$, PC III in hepatic tissue at group injected with blank plasmid. Expression quantity of mRNA of CTGF, TIMP-1, procol-${\alpha}1$ and PCIII at group A, B and C decreases, protein expression of CTGF, TIMP-1, procol-${\alpha}1$, PC III in hepatic tissue is lower, where the inhibition of combination RNA interference group (group A) on procol-${\alpha}1$ mRNA transcription and procol-${\alpha}1$ protein expression is superior to that of single interference group (group B and C) (P<0.01 or P<0.05). Conclusions: RNA interference on CTGF and/or TIMP-1 is obviously a inhibiting factor for mRNA and protein expression of CTGF, TIMP-1, procol-${\alpha}1$ and PCIII. Combination RNA interference on genes of CTGF and TIMP-1 is superior to that of single RNA interference, and this could be a contribution for prevention of precancerous condition.

• 재활복지
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• 제20권3호
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• pp.105-124
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• 2016

본 연구는 지체장애인들의 운동참여와 심박변이도(HRV), 표정정서인식력의 인과관계를 검증하는데 목적이 있다. 이를 위해 목적표집법(purposive sampling)을 이용하여, 장애인 볼링 및 론볼대회에 참여한 참가자와 충남지역의 생활체육 운동 프로그램에 참여하는 지체장애인 139명을 대상으로 하였다. 경기장과 클럽활동 시설에 직접 방문하여 연구목적을 구체적으로 설명을 한 후, 연구 참여에 동의한 대상자에 한해 운동량, 심박변이도(HRV), 표정정서인식력을 측정하였다. 측정된 결과는 SPSS 18.0, Amos 18.0 프로그램을 이용하여 평균 및 표준편차, 상관분석, 구조방정식모형을 분석되었고, 그 결과, 지체장애인들의 운동량은 자율신경계의 교감활성도와 부교감 활성도에 긍정적인 영향을 미치는 것으로 나타났다. 지체장애인들의 운동경력은 LF/HF에 긍정적인 영향을 미치는 것으로 나타났고, 부교감 활성도에는 부정적인 영향을 미치는 것으로 나타났다. 지체장애인의 교감활성도는 기쁨 정서인식력에 긍정적인 영향을 미치는 것으로 나타났으며, 운동량은 슬픔 정서인식력에 부정적인 영향을 미치는 것으로 나타났다. 이러한 연구결과가 지체장애인의 자율신경계와 표정정서인식력에 어떻게 관련성이 있는지 그 메카니즘에 대해 논의되었다.

• Journal of Plant Biotechnology
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• 제6권1호
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• pp.15-19
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• 2004

The expression of a chimeric transgene (nos-npt II) has been examined in 9 years old transgenic poplars (Populus koreana x P. nigra) growing in a nursery. The expression of the gene in twenty six independentely transformed plants were examined by 1) enzyme (NPT II) assay, 2) RT-PCR, and 3) resistance to kanamycin. High NPT II activities in young leaves of all the transformed plants were found even without a selection pressure for antibiotics for 9 years. However, the activity varied with the positions of leaves in the stem in that young leaves showed higher activity than did mature tissues. When leaf segments were cultured in the presence of 150 mg/l kanamycin, only those from young leaves produced vigorously growing callus. However, as in the case of NPTII assay, the leaf segments from mature leaves did not form callus well on the media. RT-PCR with nptII specific primers also showed that amplification products were observed only when RNAs from young tissues were used. The total RNA gel showed that while RNA in young leaves are relatively stable and in a large quantity, those in old leaves were mostly degraded. All the above results suggest that the gene is transcriptionally active only in young tissue even though it is attached to a constituitive promoter. Therefore, the expression of foreign gene in poplar plants seemed to be affected by the metabolic state of the cells and thus vary greatly with the developmental stages and the age of tissue.

• 한국어병학회지
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• 제33권1호
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• pp.35-43
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• 2020

Hypoxia is a serious problem in the marine ecosystem causing a decline in aquatic resources. MicroRNAs (miRNAs) regulate the expression of genes through binding to the corresponding sequences of their target mRNAs. Especially, miRNAs in the cytoplasm can be secreted into body fluids, which called circulating miRNAs, and the availability of circulating miRNAs as biomarkers for hypoxia has been demonstrated in mammals. However, there has been no report on the hypoxia-mediated changes in the circulating miRNAs in fish. miR-210 is known as the representative hypoxia-responsive circulating miRNA in mammals. To know whether fish miR-210 also respond to hypoxia, we analyzed the change of circulating miR-210 quantity in the serum of olive flounder (Paralichthys olivaceus) in response to hypoxia. The expression of hypoxia related genes, hypoxia inducible factor 1α (HIF-1α) and the heat shock protein 90α (HSP90α) was also analyzed. Similar to the reports from mammals, miR-210-5p and miR-210-3p were significantly increased in the serum of olive flounder in response to hypoxia, suggesting that circulating miR-210 levels in the serum can be used as a noninvasive prognostic biomarker for fish suffered hypoxia. The target genes of miR-210 were related to various biological processes, which explains the major regulatory role of miR-210 in response to hypoxia. The expression of HIF-1α and HSP90α in the tissues was also up-regulated by hypoxia. Considering the critical role of HIF-1α in miR-210 expression and HSP90 in miRNAs function, the present up-regulation of HIF-1α and HSP90α might be related to the increase of circulatory miR-210, and the interaction mechanism among HIF-1α, HSP90α, and hypoxia-responsive microRNAs in fish should be further studied.

• 한국발생생물학회지:발생과생식
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• 제22권3호
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• pp.289-295
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• 2018

Large quantity of eggs fail to be fertilized and many of fertilized eggs are unable to hatch in the eel, Anguilla japonica. Larvae of eel absorb egg yolk up to 8 days after hatching but the majority of hatched larvae die before they reach the stage of first feeding in this species. Genes of key enzymes for yolk processing (cathepsin B, D, L and lipoprotein lipase - abbreviated as ctsb, ctsd, ctsl and lpl, respectively) could be associated with egg quality. In this study, we investigated differences in the expression of these genes between floating eggs and sinking eggs, and also the relationship between the gene expressions of the enzymes and fertilization rates in the fertilized eggs obtained from artificially matured female eels. Expressions of yolk processing enzyme genes did not show significant difference between floating and sinking egg groups. Expression of ctsb decreased when fertilization rate was high. Expression of ctsd, ctsl and lpl, however, did not show any significant differences. These results suggest that ctsb expression could be an indicator of egg quality, and that some proteins prone to be digested by ctsb could be very important in the process of fertilization and normal cleavage in this species. Further study should identify these critical proteins to improve our understanding on the quality of fish eggs.

• 생체재료학회지
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• 제22권4호
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• pp.337-345
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• 2018

Background: Human mesenchymal stem cells (hMSCs) are, due to their pluripotency, useful sources of cells for stem cell therapy and tissue regeneration. The phenotypes of hMSCs are strongly influenced by their microenvironment, in particular the extracellular matrix (ECM), the composition and structure of which are important in regulating stem cell fate. In reciprocal manner, the properties of ECM are remodeled by the hMSCs, but the mechanism involved in ECM remodeling by hMSCs under topographical stimulus is unclear. In this study, we therefore examined the effect of nanotopography on the expression of ECM proteins by hMSCs by analyzing the quantity and structure of the ECM on a nanogrooved surface. Methods: To develop the nanoengineered, hMSC-derived ECM, we fabricated the nanogrooves on a coverglass using a UV-curable polyurethane acrylate (PUA). Then, hMSCs were cultivated on the nanogrooves, and the cells at the full confluency were decellularized. To analyze the effect of nanotopography on the hMSCs, the hMSCs were re-seeded on the nanoengineered, hMSC-derived ECM. Results: hMSCs cultured within the nano-engineered hMSC-derived ECM sheet showed a different pattern of expression of ECM proteins from those cultured on ECM-free, nanogrooved surface. Moreover, hMSCs on the nano-engineered ECM sheet had a shorter vinculin length and were less well-aligned than those on the other surface. In addition, the expression pattern of ECM-related genes by hMSCs on the nanoengineered ECM sheet was altered. Interestingly, the expression of genes for osteogenesis-related ECM proteins was downregulated, while that of genes for chondrogenesis-related ECM proteins was upregulated, on the nanoengineered ECM sheet. Conclusions: The nanoengineered ECM influenced the phenotypic features of hMSCs, and that hMSCs can remodel their ECM microenvironment in the presence of a nanostructured ECM to guide differentiation into a specific lineage.

• 대한기계학회논문집B
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• 제27권8호
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• pp.1071-1080
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• 2003

A national standard system was developed in order to calibrate and test the oil flowmeters for the petroleum field. A stop valve and a gyroscopic weighing scale were employed for the primary standard of the flow quantity. It is operated by the standing start and finish mode and the static weighing method. The model equation for uncertainty evaluation was based on the calibration principle of standard system. The sources of the uncertainties were quantified and combined according to the GUM(Guide to the Expression of Uncertainty in Measurement). It was found that the standard system had the relative expanded uncertainty of 0.04 % in the range of 18 - 350 ㎥/ｈ. According to the uncertainty budget, the uncertainties of the fluid density and the volume of pipeline, which were temperature dependent, contributed 92% of final uncertainty in the oil flow standard system.