• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.44 no.2
• /
• pp.166-170
• /
• 1999

The identification of quantitative trait loci (QTL) has the potential to enhance the efficiency of im- proving food processing traits of soybean. In this study, 92 restriction fragment length polymorphism (RFLP) loci and two morphological markers (W$_1$ and T) were used to identify QTL associated with food processing traits of soybean for sprout in 83 F$_2$-derived lines from a cross of ＇Pureun＇ x ＇Jinpum 2＇. The genetic map consisted of 76 loci which covered about 760 cM and converged into 20 linkage groups. Eighteen markers remained unlinked. Phenotypic data were collected for hypocotyl length, abnormal seedling rate, and sprout yield seven days after seed germination at 2$0^{\circ}C$. Based on the single-factor analysis of variance, eight independent markers were associated with hypocotyl length. Four of seven markers associated with abnormal seedling rate were identified as independent. Seven loci were associated with sprout yield. For three different traits, much of genetic variation was explained by the identified QTL in this population. Several RFLP markers in linkage group (LG) Bl were detected as being associated with three traits, providing a genetic explanation for the biological correlation of sprout yield with hypocotyl length (r=OA07＊＊＊) and with abnormal seedling rate (r=-406＊＊＊).

• Proceedings of the KOSOMBE Conference
• /
• v.1997 no.11
• /
• pp.146-149
• /
• 1997

Computerized densitometry was developed or the quantitative measurement of diffuse retinal nerve fiber layer (RNFL) atrophy and intra- and inter-operator reliability and clinical validity of this system were evaluated. Vertical diameter, center of the optic disc, and peripapillary circles which had radii of 1.5 and 2.5 times that of the optic disc were user-interactively determined in digitized RNFL photograph and density profile along each circle was measured and normalized. The areas under the normalized density profiles of the superior and the inferior segments in both circle were used or the study of RNFL. To determine the variability and correspondence in the measurements of density variations, 21 RNFL photographs of glaucoma patients which showed varying degrees of atrophy underwent computerized densitometry by two operators on two separate occasions. Coefficient of variation in the densitometric measurements was $1.2{\sim}5.4%$. Intra- and inter-operator reliabilities were excellent. The correlations between the densitometric values and mean deviations of Humphrey C30-2 visual field showed statistical significance. Computerized densitometry of RNFL photographs was useful in the objective and quantitative assessment of diffuse RNFL atrophy.

• Genomics & Informatics
• /
• v.21 no.2
• /
• pp.24.1-24.7
• /
• 2023

Assays of clinical diagnosis and species identification using molecular markers are performed according to a quantitative method in consideration of sensitivity, cost, speed, convenience, and specificity. However, typical polymerase chain reaction (PCR) assay is difficult to quantify and have various limitations. In addition, to perform quantitative analysis with the quantitative real-time PCR (qRT-PCR) equipment, a standard curve or normalization using reference genes is essential. Within the last a decade, previous studies have reported that the digital PCR (dPCR) assay, a third-generation PCR, can be applied in various fields by overcoming the shortcomings of typical PCR and qRT-PCR assays. We selected Stilla Naica System (Stilla Technologies), Droplet Digital PCR Technology (Bio-Rad), and Lab on an Array Digital Real-Time PCR analyzer system (OPTOLANE) for comparative analysis among the various droplet digital PCR platforms currently in use commercially. Our previous study discovered a molecular marker that can distinguish Hanwoo species (Korean native cattle) using Hanwoo-specific genomic structural variation. Here, we report the pros and cons of the operation of each dPCR platform from various perspectives using this species identification marker. In conclusion, we hope that this study will help researchers to select suitable dPCR platforms according to their purpose and resources.

• Tribology and Lubricants
• /
• v.21 no.5
• /
• pp.236-240
• /
• 2005

In order to prepare for the suitable surfaces of implants or medical devices, quantitative evaluation of adhesion between cells and biomaterials is essential. To better understand adhesion formation between cells and biomaterials, we used the cytodetachment technique which measures the adhesive force of a single cell through changing the, culture time and detachment speed. The results showed that the adhesive force could be affected by the culture time of cells on the surface of materials and the detachment speed. Moreover, there was a large discrepancy among the adhesion strength measured by similar techniques conducted on the different cells and substrates. It can be 'concluded that the variation of the force measurement technique can seriously alter the level of the force required to detach a cell on the surface of materials.

• Journal of the Korean Institute of Telematics and Electronics B
• /
• v.30B no.9
• /
• pp.84-93
• /
• 1993

Recently, several nonlinear shape normalization methods have been proposed in order to compensate for the shape distortions in handwritten characters. In this paper, we review these nonlinear shape normalization methods from the two points of view : feature projection and feature density equalization. The former makes feature projection histogram by projecting a certain feature at each point of input image into horizontal-or vertical-axis and the latter equalizes the feature densities of input image by re-sampling the feature projection histogram. A systematic comparison of these methods has been made based on the following criteria: recognition rate, processing speed, computational complexity and measure of variation. Then, we present the result of quantitative evaluation of each method based on these criteria for a large variety of handwritten Hangul syllables.

• Genomics & Informatics
• /
• v.14 no.4
• /
• pp.211-215
• /
• 2016

The alteration of alternative splicing patterns has an effect on the quantification of functional proteins, leading to phenotype variation. The splicing quantitative trait locus (sQTL) is one of the main genetic elements affecting splicing patterns. Here, we report the results of genome-wide sQTLs across 141 strains of Arabidopsis thaliana with publicly available next generation sequencing datasets. As a result, we found 1,694 candidate sQTLs in Arabidopsis thaliana at a false discovery rate of 0.01. Furthermore, among the candidate sQTLs, we found 25 sQTLs that overlapped with the list of previously examined trait-associated single nucleotide polymorphisms (SNPs). In summary, this sQTL analysis provides new insight into genetic elements affecting alternative splicing patterns in Arabidopsis thaliana and the mechanism of previously reported trait-associated SNPs.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2004.10a
• /
• pp.860-864
• /
• 2004

This article discusses the use of pulse-echo ultrasonic testing for the stress analysis of adhesive bonds between metal sheets. The method is based on the measurement of the reflection wave at the metal/adhesive interface. After describing briefly the physical aspects of the phenomenon, an index is defined to detect defective zone of the joint(both for the lack of adhesive and for insufficient adhesion); the influence of the experimental variables(variables stress...) on the measurement is discussed. By means of a control experiment it is shown that Stress Variation in Adhesive Joints are separate to be distingguished. In this paper, Quantitative Nondestructive Evaluation in Adhesive Joints are together with Ultrasonic Testing and Finite Element Method.

• Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
• /
• 2004.11a
• /
• pp.362-366
• /
• 2004

Quantitative evaluation of substrates for cells is essential to understanding cell-material adhesive interaction and it is also necessary for the development of new biomaterials. Many cells on adhesive molecules will form an organization of actin into bundles and production of the large, highly organized structures termed focal adhesions. To better understand adhesion formations between cells and substrata, we have quantified the force required to displace attached cell. we allowed rabbit knee chondrocyte to attach on a substratum of microscope slide glass. Our results demonstrate that a force is required to detach cells is changed according to detachment time variation.

• Genomics & Informatics
• /
• v.10 no.4
• /
• pp.234-238
• /
• 2012

Genetic epidemiology studies have established that the natural variation of gene expression profiles is heritable and has genetic bases. A number of proximal and remote DNA variations, known as expression quantitative trait loci (eQTLs), that are associated with the expression phenotypes have been identified, first in Epstein-Barr virus-transformed lymphoblastoid cell lines and later expanded to other cell and tissue types. Integration of the eQTL information and the network analysis of transcription modules may lead to a better understanding of gene expression regulation. As these network modules have relevance to biological or disease pathways, these findings may be useful in predicting disease susceptibility.

• Animal cells and systems
• /
• v.16 no.5
• /
• pp.425-430
• /
• 2012

The southwestern Australian frog Crinia georgiana (Anura; Myobatrachidae) inhabits ephemeral pools in which the tadpoles often face desiccation. Under these conditions selection on tadpoles can be severe and can directly affect fitness during the aquatic as well as the terrestrial developmental stages. A quantitative genetic study using a half-sib breeding design was conducted to understand the genetic effects on larval life-history traits. We found no significant additive genetic variance in any of larval traits. Except for hatching period, heritability estimates based on females were high in egg size, larval period, snout-vent length, and weight at metamorphosis, suggesting non-additive genetic effects. These results indicate that any response to selection during hatching and larval periods should be predominately governed by non-additive genetic effects in C. georgiana.