• 한국안전학회지
• /
• 제15권4호
• /
• pp.134-138
• /
• 2000

Signal words such as DANGER, WARNING, CAUTION, etc. have been used in order to transmit a potential hazard easily and quickly. But they were applied to a number of the sites without consistency. Thus, this study took Quantification Method and Cluster Analysis in order to judge the signal words corresponding to the urgency of situations, and to analyze whether signal words are used properly or not. According to the result of Quantification Method II signal words were most affected by Understanding, Severity and Likelihood in both student group and industrial worker group. And in Quantification Method III CAUTION corresponded to Immediacy and Understanding whereas NOTICE did to Receptivity, WARNING, DEADLY and DANCER did to Likelihood, Dangerousness and Severity. Finally, Cluster Analysis showed that CAUTION and NOTICE were recognized as similar words.

• Journal of Microbiology and Biotechnology
• /
• 제21권1호
• /
• pp.100-108
• /
• 2011

A method for the rapid detection and quantification of Newcastle disease virus (NDV) produced in an animal cell culture-based production system was developed to enhance the speed of the NDV vaccine manufacturing process. A SYBR Green I-based real-time RT-PCR was designed with a conventional, inexpensive RT-PCR kit targeting the F gene of the NDV LaSota strain. The method developed in this study was validated for specificity, accuracy, precision, linearity, limit of detection (LOD), limit of quantification (LOQ), and robustness. The validation results satisfied the predetermined acceptance criteria. The validated method was used to quantify virus samples produced in an animal cell culture-based production system. The method was able to quantify the NDV samples from mid- or late-production phases, but not effective on samples from the early-production phase. For comparison with other quantifiable methods, immunoblotting, plaque assay, and tissue culture infectious dose 50 ($TCID_{50}$) assay were also performed with the NDV samples. The results demonstrated that the real-time RT-PCR method is suitable for the rapid quantification of virus particles produced in an animal cell-culture-based production system irrespective of viral infectivity.

• 한국산업정보학회논문지
• /
• 제14권5호
• /
• pp.281-288
• /
• 2009

본 논문에서는 수량화 방법과 AHP(Analytic Hierarchy Process) 기법을 사용하여 산사태 발생에 대한 통계적 예측모형을 구축하는데 목적이 있다. 수량화(Quantification) 방법은 질적변수에 수량을 부여하는 통계적 방법으로, 기 조사된 자료에 기반하여 분석을 수행하는 방법이다. 본 논문에서는 서구의 다변량분석 기법인 정준상관분석의 결과를 토대로 수량화 과정을 구체적으로 제안한다. 데이터에 기반한 수량화 방법과는 팔리 AHP 기법은 일종의 다기준 의사결정을 위해 사용되는 기법으로, 설문자료에 기반한 분석법이다. 실제자료에 대한 분석으로 산사태 발생여부를 측정한 자료(한국지질자원연구원 제공)와 전문가 설문을 통해 수집된 자료를 이용하였다. 이들 자료에 대해 수량화 분석과 AHP분석을 통해 산사태 발생여부를 예측할 수 있는 두 종류의 평가표와 함께 로지스틱 회귀를 통한 통계적 예측모형을 개발하였으며, 두 모형간의 성능비교와 안정성 평가를 수행하였다.

• 한국미생물학회:학술대회논문집
• /
• 한국미생물학회 2003년도 International Meeting of the Microbiological Society of Korea
• /
• pp.118-121
• /
• 2003

A method for detection and quantification of aceticlastic methanogens using a real-time PCR with a TaqMan probe was developed. Two sets of primers and probes targeting the family Methanosarcinaceae and Methanosaetaceae were designed by using the Ribosormal Database Project (RDP) II, and softwares for phylogenetic probe design and sequence analysis. Target-group specificity of each set of primers and probe was verified by testing DNAs isolated from pure cultures of 28 archaeal strains purchased from DSMZ. Cell numbers in the 28 archaeal cultures and in the samples from anaerobic processes were quantified using a real-time PCR with the sets of primers and probe. In conclusion, the real-time PCR assay was very specific for the corresponding target methanogenic family and was proved to be a powerful method for quantification of aceticlastic methanogens in anaerobic processes.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권1호
• /
• pp.343-347
• /
• 2014

Background: Maspin expression is a potential prognostic factor for various malignancies but its relation with gallbladder cancer is unknown and needs to be investigated needs to be investigated. We therefore here focused on maspin mRNA expression in normal, gall stone disease and gallbladder cancer subjects, with particular attention to prognostic importance in individuals with malignancies. Materials and Methods: This study was carried out at the Department of Surgical Gastroenterology, King George's Medical University, Lucknow, India. Gallbladder samples from normal (n=25), gall stone disease (n=25) and cancer patients (n=38) were analysed for maspin mRNA expression by semi-quantitative reverse transcriptase PCR and quantitative real time PCR. Statistical analysis was carried out using the Students t test or ANOVA. Survival analysis was conducted according to the Kaplan-Meier method and correlations were assessed using the Pearson correlation method. p<0.05 was considered statistically significant. Results: Significant increase (p=0.028) in expression of maspin mRNA was observed in gallbladder cancer as compared to gall stone disease, whereas no expression was found in normal tissues. Significant correlation (Pearson's coefficient(r)=-0.798, p<0.0001) was observed between relative quantification of maspin mRNA and survival of cancer patients after surgery, with significantly shorter (p=0.002) survival in patients having relative quantification >1.5 as compared to those having relative quantification <1.5. Similarly, significant differences in patient survival for maspin mRNA expression was observed for stage II (p=0.025) and III (p=0.011) cancer. Conclusions: Higher expression of maspin mRNA in gallbladder cancer has prognostic significance for stage II and III cancer, which needs to be investigated further.

• Earthquakes and Structures
• /
• 제22권1호
• /
• pp.83-94
• /
• 2022

In recent years, some studies have identified and quantified factors that can increase or decrease the seismic vulnerability of buildings. These modifier factors, related to the building characteristics and condition, are taken into account in the vulnerability assessment, by means of a numerical estimation resulting from the quantification of these modifiers through vulnerability indexes. However, views have differed on the definition and the quantification of modifiers. In this study, modifier parameters and scores of the Risk-UE Level 1 method are adjusted based on the Algerian seismic code recommendations and the reviews proposed in the literature. The adjusted modifiers and scores are applied to reinforced concrete (RC) buildings in Boumerdes city, in order to assess probable seismic damage. Comparison between estimated damage and observed damage caused by the 2003 Boumerdes earthquake is done, with the objective to (i) validate the model involving influence of the modifier parameters on the seismic vulnerability, and (ii) to define the relationship between modifiers and damage. This research may help planners in improving seismic regulations and reducing vulnerability of existing buildings.

• 한국터널지하공간학회 논문집
• /
• 제8권2호
• /
• pp.183-196
• /
• 2006

카메라 방식의 3D 스캐너로부터 구한 암석 절리면의 거칠기를 만곡과 요철의 거칠기 성분으로 구분하였다. 거칠기의 구분 기준은 이전의 연구에서 도출된 것을 적용하였으며, 구분 방법으로는 디지털 필티링 기법을 사용하였다. 원래의 프로파일과 구분한 프로파일을 사용하여 금속 주형을 제작하였으며, 정밀한 가공을 위하여 WEDM(Wire-cut Electric Discharge Machining)을 사용하였다. 고강도 석고를 사용하여 시편을 복제하였고, 절리면 전단시험을 실시하였다. 낮은 연직응력 범위와 높은 연직응력 범위에서 전단시험을 실시하고, 거칠기의 규모와 연직응력에 따른 발현 특성을 분석하였다. 절리면 전단강도 예측을 위한 새로운 상관식을 제안하였으며, 이 상관식은 거칠기의 발현 특성과 거칠기 계수의 특성을 모두 효과적으로 고려할 수 있었다. 그러므로 거칠기의 구분에 의한 정량화가 절리면 전단강도 예측을 위한 효과적인 방법이 될 수 있음을 확인할 수 있었다.

• 분석과학
• /
• 제36권1호
• /
• pp.1-11
• /
• 2023

A new, sensitive, and reliable flow injection methodology was investigated for the determination of lead ion (II) in vegetables' samples using a laboratory-prepared reagent 2-[(6-methoxy-2-benzothiazoly)azo]-4-methoxy phenol (6-MBTAMP). Infrared spectroscopy, UV-visible spectrophotometry, Energy dispersive X-ray spectroscopy (EDX), Elemental Analysis (CHN), nuclear magnetic resonance spectroscopy ¹HNMR, and ¹³CNMR techniques were used to characterize the reagent and lead (II) complex. The method is based on lead ion (II) reacting with the reagent (6-MBTAMP) in a neutral solution to produce a green-red complex with a maximum absorbance at 670 nm. The optimum conditions, such as flow rate, lead ion (II) volume, reagent volume, medium pH, reagent concentration, and reaction coil length were thoroughly examined. The limits of detection (LOD) and quantification (LOQ) were determined to be 0.621 mg·L^-1 and 2.069 mg·L^-1 , respectively, while Sandell's sensitivity was determined to be 0.345 ㎍·cm^-2.

• 한국산림과학회지
• /
• 제97권4호
• /
• pp.437-444
• /
• 2008

본 연구는 자연휴양림 내에서 휴양지구를 설정하기 위한 방법을 모색하기 위하여 산림휴양기능의 평가를 시도하였다. 청옥산 자연휴양림을 연구대상지로 선정하고, 표준지 173개 소반을 추출하여 수량화II류를 이용하여 분석하였다. 이때 외적기준은 산림휴양 효용의 정도를 전문가로부터 부여받았고, 설명변수는 기존의 연구결과와 대상지의 산림휴양자원을 고려하여 임상, 임령, 경사, 수변, 도로, 시설의 6인자를 선정하였다. 그 결과, 절대판별적중률은 제 1 2상관비 축에서 각각 82%, 75%로서 예측 가능한 판별 정도(精度)를 얻었다. 요인으로서는 도로와 경사가 산림휴양기능의 잠재력에 큰 영향을 미치고 있으며, 카테고리에 있어서는 도로가 있는 것이, 경사는 낮을수록 산림휴양기능을 높이는 방향으로 작용하는 것을 알 수 있었다. 또한, 청옥산 자연휴양림의 소반별 산림휴양기능의 잠재력을 평가하기 위하여 표준지에서의 예측식을 사용하여 산림휴양효용의 정도를 나타내는 종합점수를 구한 다음, 판별구분점에 의하여 높다(H), 보통(M), 낮다(L)로 3구분하였으며, GIS를 이용하여 분포를 나타내는 평가도를 작성하였다. 수량화라는 형식을 매개로 하여 산림휴양의 효용의 정도에 관한 현실의 직관적 경험적 종합판단을 평가인자와 카테고리가 개입된 분석적 판단으로 변환하고 있는 점에 본 연구의 의의가 있다.

• Advances in aircraft and spacecraft science
• /
• 제4권6호
• /
• pp.613-637
• /
• 2017

The development systems for the Structural Health Monitoring has attracted considerable interest from several engineering fields during the last decades and more specifically in the aerospace one. In fact, the introduction of those systems could allow the transition of the maintenance strategy from a scheduled basis to a condition-based approach providing cost benefits for the companies. The research presented in this paper consists of a definition and next comparison of four methods applied to numerical measurements for the extraction of damage features. The first method is based on the determination of the Structural Intensity field at the on-resonance condition in order to acquire information about the dissipation of vibrational energy throughout the structure. The Damage Quantification Indicator and the Average Integrated Global Amplitude Criterion methods need the evaluation of the Frequency Response Function for a healthy plate and a damaged one. The main difference between these two parameters is their mathematical definition and therefore the accuracy of the scalar values provided as output. The fourth and last method is based on the Mode-shape Curvature, a FRF-based technique which requires the application of particular finite-difference schemes for the derivation of the curvature of the plate. All the methods have been assessed for several damage conditions (the shape, the extension and the intensity of the damage) on two test plates: an isotropic (steel) plate and a 4-plies composite plate.