• Journal of Microbiology and Biotechnology
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• v.4 no.3
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• pp.183-190
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• 1994

Cell-free extracts prepared from cultured insect cells, Spodoptera. frugiperda, were analyzed for activation of early gene transcription of an insect baculovirus, Autographa californica nuclear polyhedrosis virus (AcNPV). The template DNA used for in vitro transcription assays contained promoter sites for the baculovirus genes that have been classified as immediate early ($\alpha$) or early genes. These genes are located in the HindIII-K/Q region of the AcNPV genome. Nuclei isolated from the AcNPV-infected Spodoptera frugiperda cells were also used for in vitro transcription analysis by RNase-mapping the labeled RNA synthesized from in vitro run-on reaction in the isolated nuclei. The genes studied by this technique were p26 and pl0 genes which were classified as delayed early and late gene, respectively. We found that transcription of the genes from the HindIII-K region was accurately initiated and unique in the whole cell extract obtained from uninfected cells, although abundance of the in vitro transcripts was reverse to that of in vivo RNA. With isolated nuclei transcription of the p26 gene was inhibited by $\alpha$-amanitin suggesting that the p26 gene was transcribed by host RNA polymerase II. However, transcription of the pl0 gene in isolated nuclei was not inhibited by $\alpha$-amanitin, but rather stimulated by the inhibitor. We also found that the synthesis of $\alpha$-amanitin-resistant RNA polymerase was begun before 6 hr p.i., the time point at which the onset of viral DNA replication as well as the appearance of a-amanitin-resistant viral transcripts were detected. These studies give us strong evidence to support the previous data that early genes of AcNPV were transcribed by host RNA polymerease III, while transcription of late genes was mediated at least by a novel $\alpha$-amanitin-resistant RNA polymerase.

• International Journal of Automotive Technology
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• v.8 no.3
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• pp.375-382
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• 2007

The multi-driver off-road vehicle drive-line consists of many components, with close connections among them. In order to design and analyze the drive-line efficiently, a modular methodology should be taken. The aim of a modular approach to the modeling of complex systems is to support behavior analysis and simulation in an iterative and thus complex engineering process, by using encapsulated submodels of components and of their interfaces. Multi-driver off-road vehicles are comparatively complicated. The driving-line is an important core part to the vehicle, it has a significant contribution to the performance. Multi-driver off-road vehicles have complex driving-lines, so performance is heavily dependent on the driving-line. A typical off-road vehicle's driving-line system consists of a torque converter, transmission, transfer case and driving-axles, which transfers the power generated by the engine and distributes it effectively to the driving wheels according to the road condition. According to its main function, this paper proposes a modularized approach for design and evaluation of the vehicle's driving-line. It can be used to effectively estimate the performance of the driving-line during the concept design stage. Through an appropriate analysis and assessment method, an optimal design can be reached. This method has been applied to practical vehicle design, it can improve the design efficiency and is convenient to assess and validate the performance of a vehicle, especially of multi-driver off-road vehicles.

• Journal of Environmental Impact Assessment
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• v.3 no.1
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• pp.53-68
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• 1994

Initially the paper will explore the concept, evolution and utility of Sustainable Development in the broad context of its links to the EIA process by predicting in advance decisions being made attempts to balance the needs of environmental, economic and social objectives. The paper then considers how an evolving and improved EIA process and in particular with the emphasis increasingly riving from projects to polieies, plans and programmes, can provide q framework to operationalise Sustainable Development with the focus on cumulative impacts, making EIA "problem focussed" and linking policy, environmental planning and assessment. The paper then reviews some of the practical EIA tools and methods that can support the practical application of more susk inable forms of development. The paper concludes by considering the follow ups that are now occurring after the Reo Sumit and suggests that has a Key and powerful role to play in this process, whether at an institutional prettiest policy level.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.16
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• pp.6863-6869
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• 2014

Objectives: Several preclinical and observational studies have shown that anti-diabetic medications (ADMs) may modify the risk of lung cancer. We performed a systematic review and meta-analysis evaluating the effect of metformin, sulfonylureas (SUs), thiazolidinediones (TZDs), and insulin on the risk of lung cancer in patients with diabetes mellitus (DM). Materials and Methods: We conducted a systematic search of Pubmed and Web of Science, up to August 20, 2013. We also searched the Conference Proceedings Citation Index (CPCI) and China National Knowledge Infrastructure (CNKI) for abstracts from major meetings. Fixed or random effect pooled measures were selected based on heterogeneity among studies, which was evaluated using Q test and the I2 of Higgins and Thompson. Meta-regression was used to explore the sources of between-study heterogeneity. Publication bias was analyzed by Begg's funnel plot and Egger's regression test. Associations were assessed by odds ratios (ORs) with 95% confidence intervals (CIs). Results: A total of 15 studies (11 cohort, 4 case-control) were included in this meta-analysis. In observational studies no significant association between metformin (n=11 studies; adjusted OR=0.99, 95%CI: 0.87-1.12), SUs (n=5 studies; adjusted OR=0.98, 95%CI: 0.79-1.22), or TZDs (n=7 studies; adjusted OR=0.92, 95%CI: 0.75-1.13), insulin (n=6 studies; adjusted OR=1.13, 95%CI: 0.79-1.62) use and risk of developing lung cancer was noted. There was considerable inherent heterogeneity between studies not explained by study design, setting, or location. Conclusions: Meta-analysis of existing studies does not support a protective or harmful association between ADMs use and risk of lung cancer in patients with DM. There was considerable heterogeneity across studies, and future, well-designed, prospective studies would be required for better understanding of any association.

• Journal of the Korean Data and Information Science Society
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• v.22 no.3
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• pp.495-503
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• 2011

The most widely used data mining technique is to find association rules. Association rule mining is the method to quantify the relationship between each set of items in very huge database based on the association thresholds. There are some basic association thresholds to explore meaningful association rules ; support, confidence, lift, etc. Among them, confidence is the most frequently used, but it has the drawback that it can not determine the direction of the association. The net confidence and the attributably pure confidence were developed to compensate for this drawback, but they have other drawbacks.In this paper we consider some predictive similarity measures for binary data in cluster analysis and multi-dimensional analysis as association threshold to compensate for these drawbacks. The comparative studies with net confidence, attributably pure confidence, and some predictive similarity measures are shown by numerical example.

• Molecular & Cellular Toxicology
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• v.5 no.4
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• pp.310-316
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• 2009

Some environmental chemicals have been shown to cause liver-toxicity as the result of bioaccumulation. Particularly, fungicides have been shown to cause varying degrees of hepatictoxicity and to disrupt steroid hormone homeostasis in in vivo models. The principal objective of this study was to evaluate the liver-toxic responses of environmental chemicals-in this case selected fungicides and parasiticides-in order to determine whether or not this agent differentially affected its toxicogenomic activities in hepatic tumor cell lines. To determine the gene expression profiles of 3 fungicides (triadimefon, myclobutanil, vinclozolin) and 1 parasiticide (dibutyl phthalate), we utilized a modified HazChem human array V2. Additionally, in order to observe the differential alterations in its time-dependent activities, we conducted two time (3 hr, 48 hr) exposures to the respective IC20 values of four chemicals. As a result, we analyzed the expression profiles of a total of 1638 genes, and we identified 70 positive significant genes and 144 negative significant genes using four fungicidic and parasiticidic chemicals, using SAM (Significant Analysis of Microarray) methods (q-value<0.5%). These genes were analyzed and identified as being related to apoptosis, stress responses, germ cell development, cofactor metabolism, and lipid metabolism in GO functions and pathways. Additionally, we found 120 genes among those time-dependently differentially expressed genes, using 1-way ANOVA (P-value<0.05). These genes were related to protein metabolism, stress responses, and positive regulation of apoptosis. These data support the conclusion that the four tested chemicals have common toxicogenomic effects and evidence respectively differential expression profiles according to exposure time.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.7
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• pp.3605-3610
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• 2016

Background: Histone acetylation in chromatin structures plays a key role in regulation of gene transcription and is strictly controlled by histone acetyltransferase (HAT) and deacetylase (HDAC) activities. HDAC deregulation has been reported in several cancers. Materials and Methods: The expression of 10 HDACs (including HDAC class I and II) was studied by quantitative reverse transcription-PCR (qRT-PCR) in a cohort of mesenchymal stem cells (MM-MSCs) from 10 multiple myeloma patients with a median age 60y. The results were compared with those obtained for normal donors. Then, a coculture system was performed between MM-MSCs and u266 cell line, in the presence or absence of sodium butyrate (NaBT), to understand the effects of HDAC inhibitors (HDACi) in MM-MSCs on multiple myeloma cases. Also, the interleukin-6 (IL-6) and vascular endothelial growth factor (VEGFA) gene expression level and apoptotic effects were investigated in MM-MSCs patients and control group following NaBT treatment. Results: The results indicated that upregulated (HDACs) and downregulated (IL6 and VEGFA) genes were differentially expressed in the MM-MSCs derived from patients with multiple myeloma and ND-MSCs from normal donors. Comparison of the MM-MSCs and ND-MSCs also showed distinct HDACs expression patterns. For the first time to our knowledge, a significant increase of apoptosis was observed in coculture with MM-MSCs treated with NaBT. Conclusions: The obtained findings elucidate a complex set of actions in MSCs in response to HDAC inhibitors, which may be responsible for anticancer effects. Also, the data support the idea that MSCs are new therapeutic targets as a potential effective strategy for MM.

• Journal of Korea Multimedia Society
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• v.9 no.1
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• pp.73-88
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• 2006

With the development of Next Generation convergence Network, the current Internet needs a new network architecture and new session establishment methods, which can support various applications requiring differentiated traffic processing and high quality of service instead of the conventional best-effort service. It is expected that, in next generation network based on All-IP. SIP will play an important role in the session establishment between the end to end user terminals. In this paper, we propose a new network architecture that can provide quality of service based on SIP using the QoS enabled SIP servers and policy servers, and we also propose a new session establishment method that apply the user's demands participating the session establishment when setting up the session using the SIP. Through simulations under various environments, we show the parameter ranges in terms of server capacity and the number of users in access networks to achieve reasonable delay bounds.

• BMB Reports
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• v.49 no.9
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• pp.514-519
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• 2016

Korean cattle (Hanwoo) are categorized into three breeds based on color: brown, brindle, and black. Among these breeds, brown Hanwoo has been subjected to intensive selection to improve meat traits. To identify genetic traces driven by recent selection in brown Hanwoo, we scanned the genomes of brown and brindle Hanwoo using a bovine SNP chip. We identified 17 candidate selection signatures in brown Hanwoo and sequenced four candidate regions from 10 individuals each of brown and brindle Hanwoo. In particular, non-synonymous SNPs in the ADSL gene (K88M, L189H, and R302Q) might have had mutational effects on protein structure as a result of altering the purine pathway during nucleotide breakdown. The ADSL gene was previously reported to affect meat quality and yield in livestock. Meat quality and yield are main breeding goals for brown Hanwoo, and our results support a potential causal influence of non-synonymous SNPs in the ADSL gene.

• Journal of Energy Engineering
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• v.25 no.3
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• pp.83-94
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• 2016

Energy use has been recognized worldwide as a main cause of global warming and it is at the center of climate change. In this study, problems and measures of zero-energy building construction are investigated and analyzed. Based on the results, evaluation criteria of the zero-energy building are suggested. Performance related factors(Q) representing the environmental grade were divided into three categories as outdoor, indoor environment and maintenance. Energy related factors(LR) representing the energy load were divided into an energy, materials & resources, water cycle management, land use and transportation. Detailed fifty three items are listed for the evaluation under the consideration of energy, water cycle management sections gave weight. Upon receiving the first in the environment friendly certification system, Seoul Central Post Office and Seoul Metropolitan Water Supply Center evaluated. The reason why this score difference is due to lack of use of new generation energy building construction is required expensive costs so need expansion of governmental support. This effort is successful zero energy building construction and copes with global warming and climate change.